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The map label for this gene is ica3 [H]

Identifier: 28379211

GI number: 28379211

Start: 2416287

End: 2417615

Strand: Direct

Name: ica3 [H]

Synonym: lp_2716

Alternate gene names: 28379211

Gene position: 2416287-2417615 (Clockwise)

Preceding gene: 28379210

Following gene: 28379228

Centisome position: 73.04

GC content: 45.75

Gene sequence:

>1329_bases
ATGACAAATATTTTTATTGACCACATATTCGCCGCAACCTTATTTAAATCGGTAATCAACATCGCACTCCTGATTTTATG
CCTCTACCCCATCGTTGGCTCTTTTTTCTGGTTCGCCGGCGCGCTATCCTACCGCTTTATCAAGACCAATAAGCGTGATG
ATGACTGGCAGTACATTCCGGCTGAACAGCAACCAATGATTACCATCATGATCCCGGCGCACAACGAAGAAGTCATGATT
GAAGAAACCATCACATACTTATTTACCCAGCTCAATTATACGAACTACGAGGTCCTAGTCATGAACGATGGTTCGACTGA
CAAGACGGCCACCATTATTCAACGGTTGCAGTCCGTCTATCCACGGCTACGAACCGTTGAAATTGAGAAAAACAAGGGCA
AGGCCCACGCCTTCAATATTGGCATGTACTTTGCCCAGGGTGAGTATATTCTCAGCAACGACGCTGATACGATTCCAGAA
AAGGACGCCCTGATGAAGTATATGAATTTCTTCATCCATGACCGCGATATGAATACATCCGCCGTCACCGCCAATATGGA
TGTTCAAAATCGAACGTCGCTTTTAGGTAAATCTCAAACCGTTGAATTCTCTAGTATCGTCGGTGTTATCAAGCGGAGTC
AAACTTCCATCAACGACTCTATGTACGCGTATAGCGGGGCAAATACCCTCTACCGCAAACAATTTCTAATTGACGTTGGT
GGCTTTCGACAAAATCGCGCGACGGAAGATATCAGTATTGCCTGGGATCACCAAATGGTGGGCGCCGTCCCGCGGTTTGC
CCCTAACATCGTCTTCCATATGAACGTCCCCACCACAATCCGAGACCTTTATCATCAACGCAAACGTTGGGCACAGGGTG
GCACGGAAGTGTGGTTAACGAACATTAAGAAATTCATTTTCCACCCAATCGTTCATCGCTATCAACTATCAATGTTCGTC
GATTCGACGTTGTCAATCATCTGGTCCTTTTTCTTCGTCATCACTTCCTTGATGTTTTTGATTCTGATGGGTTACTTCCT
GATAACCGGCAATTTTGAACGAGTCTACCACGGCTTGATGTTGTCATTTGTCTTTGTTAGTTTTGAACTATTGGCCGGGT
TCATGCAACTGTTAGCGGCACTCCTGTTGGACCATCATGGTGCGAAACTAAAGTACTGGTTCTTCGCCCCACTCTACATG
TTATTTTACTGGATGATCAATCCTATCACCGTCGTTGCAACGTTTATTCCTGCACTCAAAACCATTCTGGGCTTTGGCTC
CGGAACGTGGGTCAGCCCGAAGCGACAGTCATTGCAGGGTAAAAAGTAA

Upstream 100 bases:

>100_bases
CTACCGGGTCAAGCCAGAACAAAATCTCGATACTAATCAGATTCAGCAACTTTATCAACAAAATAATTTAGACGATATTA
ACGATTAACCAGGGGGCGCC

Downstream 100 bases:

>100_bases
AACGGTGTCGCTGTTAACGCTTTCAATAATCGATAACGAGTCTACTGGTGCGAGAACTTGCAACCAGCCCAACCAAAAAC
GTGCACTTACCTGAGTGATA

Product: glycosyltransferase (putative)

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTNIFIDHIFAATLFKSVINIALLILCLYPIVGSFFWFAGALSYRFIKTNKRDDDWQYIPAEQQPMITIMIPAHNEEVMI
EETITYLFTQLNYTNYEVLVMNDGSTDKTATIIQRLQSVYPRLRTVEIEKNKGKAHAFNIGMYFAQGEYILSNDADTIPE
KDALMKYMNFFIHDRDMNTSAVTANMDVQNRTSLLGKSQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKQFLIDVG
GFRQNRATEDISIAWDHQMVGAVPRFAPNIVFHMNVPTTIRDLYHQRKRWAQGGTEVWLTNIKKFIFHPIVHRYQLSMFV
DSTLSIIWSFFFVITSLMFLILMGYFLITGNFERVYHGLMLSFVFVSFELLAGFMQLLAALLLDHHGAKLKYWFFAPLYM
LFYWMINPITVVATFIPALKTILGFGSGTWVSPKRQSLQGKK

Sequences:

>Translated_442_residues
MTNIFIDHIFAATLFKSVINIALLILCLYPIVGSFFWFAGALSYRFIKTNKRDDDWQYIPAEQQPMITIMIPAHNEEVMI
EETITYLFTQLNYTNYEVLVMNDGSTDKTATIIQRLQSVYPRLRTVEIEKNKGKAHAFNIGMYFAQGEYILSNDADTIPE
KDALMKYMNFFIHDRDMNTSAVTANMDVQNRTSLLGKSQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKQFLIDVG
GFRQNRATEDISIAWDHQMVGAVPRFAPNIVFHMNVPTTIRDLYHQRKRWAQGGTEVWLTNIKKFIFHPIVHRYQLSMFV
DSTLSIIWSFFFVITSLMFLILMGYFLITGNFERVYHGLMLSFVFVSFELLAGFMQLLAALLLDHHGAKLKYWFFAPLYM
LFYWMINPITVVATFIPALKTILGFGSGTWVSPKRQSLQGKK
>Mature_441_residues
TNIFIDHIFAATLFKSVINIALLILCLYPIVGSFFWFAGALSYRFIKTNKRDDDWQYIPAEQQPMITIMIPAHNEEVMIE
ETITYLFTQLNYTNYEVLVMNDGSTDKTATIIQRLQSVYPRLRTVEIEKNKGKAHAFNIGMYFAQGEYILSNDADTIPEK
DALMKYMNFFIHDRDMNTSAVTANMDVQNRTSLLGKSQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKQFLIDVGG
FRQNRATEDISIAWDHQMVGAVPRFAPNIVFHMNVPTTIRDLYHQRKRWAQGGTEVWLTNIKKFIFHPIVHRYQLSMFVD
STLSIIWSFFFVITSLMFLILMGYFLITGNFERVYHGLMLSFVFVSFELLAGFMQLLAALLLDHHGAKLKYWFFAPLYML
FYWMINPITVVATFIPALKTILGFGSGTWVSPKRQSLQGKK

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=416, Percent_Identity=29.3269230769231, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 51027; Mature: 50896

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIFIDHIFAATLFKSVINIALLILCLYPIVGSFFWFAGALSYRFIKTNKRDDDWQYIP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
AEQQPMITIMIPAHNEEVMIEETITYLFTQLNYTNYEVLVMNDGSTDKTATIIQRLQSVY
CCCCCEEEEEEECCCCCEEHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHC
PRLRTVEIEKNKGKAHAFNIGMYFAQGEYILSNDADTIPEKDALMKYMNFFIHDRDMNTS
CCCEEEEEECCCCCEEEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
AVTANMDVQNRTSLLGKSQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKQFLIDVG
EEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHEEECC
GFRQNRATEDISIAWDHQMVGAVPRFAPNIVFHMNVPTTIRDLYHQRKRWAQGGTEVWLT
CCCCCCCCCCCEEEECCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHH
NIKKFIFHPIVHRYQLSMFVDSTLSIIWSFFFVITSLMFLILMGYFLITGNFERVYHGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LSFVFVSFELLAGFMQLLAALLLDHHGAKLKYWFFAPLYMLFYWMINPITVVATFIPALK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TILGFGSGTWVSPKRQSLQGKK
HHHCCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure 
TNIFIDHIFAATLFKSVINIALLILCLYPIVGSFFWFAGALSYRFIKTNKRDDDWQYIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCC
AEQQPMITIMIPAHNEEVMIEETITYLFTQLNYTNYEVLVMNDGSTDKTATIIQRLQSVY
CCCCCEEEEEEECCCCCEEHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHC
PRLRTVEIEKNKGKAHAFNIGMYFAQGEYILSNDADTIPEKDALMKYMNFFIHDRDMNTS
CCCEEEEEECCCCCEEEEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
AVTANMDVQNRTSLLGKSQTVEFSSIVGVIKRSQTSINDSMYAYSGANTLYRKQFLIDVG
EEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHEEECC
GFRQNRATEDISIAWDHQMVGAVPRFAPNIVFHMNVPTTIRDLYHQRKRWAQGGTEVWLT
CCCCCCCCCCCEEEECCHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHH
NIKKFIFHPIVHRYQLSMFVDSTLSIIWSFFFVITSLMFLILMGYFLITGNFERVYHGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LSFVFVSFELLAGFMQLLAALLLDHHGAKLKYWFFAPLYMLFYWMINPITVVATFIPALK
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TILGFGSGTWVSPKRQSLQGKK
HHHCCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]