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The map label for this gene is mleR [H]

Identifier: 28379191

GI number: 28379191

Start: 2386988

End: 2387884

Strand: Direct

Name: mleR [H]

Synonym: lp_2689

Alternate gene names: 28379191

Gene position: 2386988-2387884 (Clockwise)

Preceding gene: 28379189

Following gene: 28379193

Centisome position: 72.15

GC content: 46.71

Gene sequence:

>897_bases
ATGAATATTAAAGATTTGCAATATTATGTTTCGTTAACTCAGCTGAAAAATTTTTCATTAGTTGCAGCCCAATTTCACGT
TAGTCAACCGACCATTTCGGCTGCTATTCGGCGATTGGAGACCGAACTGAAAACACAATTACTAATTCGGGCTAATCCCC
ACTTACCCGTGGCACTCACCACGACCGGGATTCAAGTCCAACGACACGCCAATCAGATCCTATTAGAGCACCAACTGATG
TGCCAAGAAGTTGCCCACACCACAACCGATCAACTTGTCATCGGGATGCCGCCAATCATTGAAATCAACTACTTTCCCCG
GATTGCAAGCCAATTACCACAAGCTTTATTCAGCCAGATTCAACCTATCAGTCAAGGTTCACTCGCGGCGCTCAAAGGAT
TAAAAAACGGACAATTAGACTTAGCCGTACTTGCTTACCTCAATGAATTCACGGATACGACCATTCAACTCACGACGTTT
GACACCCAGTCATTTAGTATTGTTGTACCAACTGACGATCCGCTAGCAACCAACTCACAACTTCAGTTCAGATCGTTGCG
TCATCAGCATTTTGTGGCTCTCAAAGATAACTTTGTCCACCGCCAGGCGTTTCGTCAACTCAGTCATCAAAATCATGTTC
GGCCAACGATTGTCTTTGAATCCAATGAAATCGGCAGTATCTTAAACATGGTTCGTCAGCATGTGGGCATTGCCTTATTG
AGTAACGCCGTCCAATTACCGGCGGGATTACAGCGGCTCCCCCTAACTGACGCGCAAGCGCCCACGTTCAATGTCGGTGT
TGCGTACCGCCACTCAATGACGTTTAGTCCAACCCAAGCCGACCTGATCCAACGACTGACCGCTGCTTTTCAATCCGTCG
ATTGGCGCGCATACTAA

Upstream 100 bases:

>100_bases
GCCCTACAATAGCCACAACGATTACCATAGTCAAATAACCATGTTATAATAACAAATCATAATACTAACAATTAAGCATT
AATTAAATAAGGACTTTAAG

Downstream 100 bases:

>100_bases
CCAGCACCTAAAAATCCACCGTGTAAGTTTATCAATATGATAAGCTCACACGGTGGATTTTATAACGACTGTACGTCAAG
TTAGGTCGCCAAATGCAGGT

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNIKDLQYYVSLTQLKNFSLVAAQFHVSQPTISAAIRRLETELKTQLLIRANPHLPVALTTTGIQVQRHANQILLEHQLM
CQEVAHTTTDQLVIGMPPIIEINYFPRIASQLPQALFSQIQPISQGSLAALKGLKNGQLDLAVLAYLNEFTDTTIQLTTF
DTQSFSIVVPTDDPLATNSQLQFRSLRHQHFVALKDNFVHRQAFRQLSHQNHVRPTIVFESNEIGSILNMVRQHVGIALL
SNAVQLPAGLQRLPLTDAQAPTFNVGVAYRHSMTFSPTQADLIQRLTAAFQSVDWRAY

Sequences:

>Translated_298_residues
MNIKDLQYYVSLTQLKNFSLVAAQFHVSQPTISAAIRRLETELKTQLLIRANPHLPVALTTTGIQVQRHANQILLEHQLM
CQEVAHTTTDQLVIGMPPIIEINYFPRIASQLPQALFSQIQPISQGSLAALKGLKNGQLDLAVLAYLNEFTDTTIQLTTF
DTQSFSIVVPTDDPLATNSQLQFRSLRHQHFVALKDNFVHRQAFRQLSHQNHVRPTIVFESNEIGSILNMVRQHVGIALL
SNAVQLPAGLQRLPLTDAQAPTFNVGVAYRHSMTFSPTQADLIQRLTAAFQSVDWRAY
>Mature_298_residues
MNIKDLQYYVSLTQLKNFSLVAAQFHVSQPTISAAIRRLETELKTQLLIRANPHLPVALTTTGIQVQRHANQILLEHQLM
CQEVAHTTTDQLVIGMPPIIEINYFPRIASQLPQALFSQIQPISQGSLAALKGLKNGQLDLAVLAYLNEFTDTTIQLTTF
DTQSFSIVVPTDDPLATNSQLQFRSLRHQHFVALKDNFVHRQAFRQLSHQNHVRPTIVFESNEIGSILNMVRQHVGIALL
SNAVQLPAGLQRLPLTDAQAPTFNVGVAYRHSMTFSPTQADLIQRLTAAFQSVDWRAY

Specific function: Required for malolactic fermentation. It is most probably a transcriptional activator [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790399, Length=280, Percent_Identity=27.8571428571429, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788887, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=241, Percent_Identity=25.3112033195021, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI2367136, Length=256, Percent_Identity=22.65625, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=24.2622950819672, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33421; Mature: 33421

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKDLQYYVSLTQLKNFSLVAAQFHVSQPTISAAIRRLETELKTQLLIRANPHLPVALT
CCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCCEEEE
TTGIQVQRHANQILLEHQLMCQEVAHTTTDQLVIGMPPIIEINYFPRIASQLPQALFSQI
ECCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHC
QPISQGSLAALKGLKNGQLDLAVLAYLNEFTDTTIQLTTFDTQSFSIVVPTDDPLATNSQ
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCC
LQFRSLRHQHFVALKDNFVHRQAFRQLSHQNHVRPTIVFESNEIGSILNMVRQHVGIALL
HHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
SNAVQLPAGLQRLPLTDAQAPTFNVGVAYRHSMTFSPTQADLIQRLTAAFQSVDWRAY
HHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIKDLQYYVSLTQLKNFSLVAAQFHVSQPTISAAIRRLETELKTQLLIRANPHLPVALT
CCCCHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCCEEEE
TTGIQVQRHANQILLEHQLMCQEVAHTTTDQLVIGMPPIIEINYFPRIASQLPQALFSQI
ECCEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCHHHHHHHHHHHHHHC
QPISQGSLAALKGLKNGQLDLAVLAYLNEFTDTTIQLTTFDTQSFSIVVPTDDPLATNSQ
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCC
LQFRSLRHQHFVALKDNFVHRQAFRQLSHQNHVRPTIVFESNEIGSILNMVRQHVGIALL
HHHHHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
SNAVQLPAGLQRLPLTDAQAPTFNVGVAYRHSMTFSPTQADLIQRLTAAFQSVDWRAY
HHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2498286; 11337471 [H]