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The map label for this gene is pncA [H]

Identifier: 28379135

GI number: 28379135

Start: 2330792

End: 2331346

Strand: Direct

Name: pncA [H]

Synonym: lp_2612

Alternate gene names: 28379135

Gene position: 2330792-2331346 (Clockwise)

Preceding gene: 28379134

Following gene: 28379139

Centisome position: 70.45

GC content: 50.45

Gene sequence:

>555_bases
ATGGCAAGTCACCAAGCCTTACTGATTATTGATTATACGAATGATTTTGTGGCTGACGGCGGGGCACTCACTTGTGGGCA
ACCCGGCCAAGCGCTCGCACCAACGATCGTCAAACTCGCCAACCAAATGGCTACGGCAAGCGACTGGGTCTTACTGCCAA
CCGATGTCCATACCCCGCATGATCCTTACCATCCCGAAAGCAAGTTGTTTCCACCTCATAATGTTCGGGGTACCTGGGGT
CGTGAACTCTATGGGCCACTCAAAGATTGGTACGCTGATCATCAAGCCGCCGATAATGTTTGGGCATTCGACAAGACGCG
TTACAGTTCATTTGCGGGTACTGATTTGGATTTACGTCTGCGAGAACGCCATGTTGACACCCTGCACTTAGTCGGTGTTT
GTACCGACATTTGTGTCCTTCACACGGCTGTTGACGCCTATAATTTGGGTTACCAACTCGTCATTCATCAGGATGCCGTT
GCTAGTTTTGACGCCGCTGGTCACGAATGGGCGTTGCGTCACTTCAAAAACAGCTTAGGTGCAACCATCGTTTAA

Upstream 100 bases:

>100_bases
TAATTATTTAGTCAATCCAACGCTAGTTAAACAATTGACAGTCCCATCAGAGTTGTTTACAGTAAAACCAATCTCAAACC
TAATATTGGAGGCCTTTATT

Downstream 100 bases:

>100_bases
AAAATGAGCAATAAAAATCACTCATTCAATACGAATGAGTGATTTTTTAGGATTACTAAATTAATTCAAGGTTGGCTCAA
CCCATTTTTGCAACATCAAG

Product: pyrazinamidase/nicotinamidase

Products: nicotinate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MASHQALLIIDYTNDFVADGGALTCGQPGQALAPTIVKLANQMATASDWVLLPTDVHTPHDPYHPESKLFPPHNVRGTWG
RELYGPLKDWYADHQAADNVWAFDKTRYSSFAGTDLDLRLRERHVDTLHLVGVCTDICVLHTAVDAYNLGYQLVIHQDAV
ASFDAAGHEWALRHFKNSLGATIV

Sequences:

>Translated_184_residues
MASHQALLIIDYTNDFVADGGALTCGQPGQALAPTIVKLANQMATASDWVLLPTDVHTPHDPYHPESKLFPPHNVRGTWG
RELYGPLKDWYADHQAADNVWAFDKTRYSSFAGTDLDLRLRERHVDTLHLVGVCTDICVLHTAVDAYNLGYQLVIHQDAV
ASFDAAGHEWALRHFKNSLGATIV
>Mature_183_residues
ASHQALLIIDYTNDFVADGGALTCGQPGQALAPTIVKLANQMATASDWVLLPTDVHTPHDPYHPESKLFPPHNVRGTWGR
ELYGPLKDWYADHQAADNVWAFDKTRYSSFAGTDLDLRLRERHVDTLHLVGVCTDICVLHTAVDAYNLGYQLVIHQDAVA
SFDAAGHEWALRHFKNSLGATIV

Specific function: Unknown

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI87081992, Length=71, Percent_Identity=42.2535211267606, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.19

Molecular weight: Translated: 20357; Mature: 20226

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASHQALLIIDYTNDFVADGGALTCGQPGQALAPTIVKLANQMATASDWVLLPTDVHTPH
CCCCCEEEEEECCCCEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
DPYHPESKLFPPHNVRGTWGRELYGPLKDWYADHQAADNVWAFDKTRYSSFAGTDLDLRL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCEEHH
RERHVDTLHLVGVCTDICVLHTAVDAYNLGYQLVIHQDAVASFDAAGHEWALRHFKNSLG
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCC
ATIV
CCCC
>Mature Secondary Structure 
ASHQALLIIDYTNDFVADGGALTCGQPGQALAPTIVKLANQMATASDWVLLPTDVHTPH
CCCCEEEEEECCCCEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
DPYHPESKLFPPHNVRGTWGRELYGPLKDWYADHQAADNVWAFDKTRYSSFAGTDLDLRL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCEEHH
RERHVDTLHLVGVCTDICVLHTAVDAYNLGYQLVIHQDAVASFDAAGHEWALRHFKNSLG
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCC
ATIV
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]