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The map label for this gene is rihB [H]

Identifier: 28379119

GI number: 28379119

Start: 2309470

End: 2310426

Strand: Direct

Name: rihB [H]

Synonym: lp_2591

Alternate gene names: 28379119

Gene position: 2309470-2310426 (Clockwise)

Preceding gene: 28379116

Following gene: 28379120

Centisome position: 69.81

GC content: 48.17

Gene sequence:

>957_bases
ATGGCAAAACGTAAAATGATTTTAGACTTAGACACCGGGATTGACGACGCAATGGCCATTGCCTACGCAGTCGGTGCACC
CGACGTTGATTTAATTGGGATTATCAGTTCTTATGGGAACTGCCTAGTGGATCAGGCGGCGATTAACAGTTTACAAATTC
TAGAACTACTAGGTGCCACTGACGTACCCGTCTTTCTAGGTGAACCCCACTCCAGTACGACGGAACACTTTGACGTTATG
CCGATTTCACAGCAGATTCATGGCATGAACGGTATCGGCGACGTGAACCTTCCTGAACCGAAACGCGCCGTGGAAAAGCA
GTCCGGTGTCGACTTCTTAATTGATGCCGTTCACCAATATGGCGCCGATCTGACCCTAGTGCCAACCGGTCCCTTAACCA
ACTTGGCCGAAGCACTCGAAAAAGCACCAGACATTGCCAGCACCATTGGTAATGTGACCTTAATGGGTGGTGCACTGACT
GTTCCAGGTAATGTCAGTCACTACGCTGAAGCCAATATTAATCAAGATGCCGAAGCGGCCAACGCCGTCTTCACCAGTAC
GATGCCTCTTACGATGGTCGGACTCGACGTAACGTTACGGACATTATTAACCAAGACTGAAACACAACAATGGCGGGACT
TAAAGACAACTGCGGGCGAAAAATTTGCTGACATTGTTGACTATTACATTGCGGCTTACGATATTACGAGTCCTGACTTA
CACGGTTGTGCCTTACACGATCCACTGGCAGTCGGCGTTTCACTCGATCCAAGTTTTGTCACCACGTTAGACCTGAATAT
GTACGTCCAGGCTAGTGGTGAAGATTATGGCCGGACAATTGGTGACCCTGCGCGACTCAATGACCCAACTAACGTCACGG
TTGCGTTAACGGTCGACAAGGATCGTTATTTGAAGACATTCATGAATTACCTGACGACATTATTTAAAGCCCATTAG

Upstream 100 bases:

>100_bases
AATTTAATTCGCCGCTAGCCGTTGTCAGCATCAGCTGAAACGTTTACAATTAAAACATAGCTTTATAGCGCTTCAGACCT
AATCAGGGAGGATGTTTTAC

Downstream 100 bases:

>100_bases
TTATATTTCATCATGGCAACCGGATCACAGAAGTTCGCTTCCGTGATCCGGTTGTTATTATTTATTTACAAGTCTGCTAA
TTTTTCATTTTTCCCCGGCA

Product: purine nucleosidase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAKRKMILDLDTGIDDAMAIAYAVGAPDVDLIGIISSYGNCLVDQAAINSLQILELLGATDVPVFLGEPHSSTTEHFDVM
PISQQIHGMNGIGDVNLPEPKRAVEKQSGVDFLIDAVHQYGADLTLVPTGPLTNLAEALEKAPDIASTIGNVTLMGGALT
VPGNVSHYAEANINQDAEAANAVFTSTMPLTMVGLDVTLRTLLTKTETQQWRDLKTTAGEKFADIVDYYIAAYDITSPDL
HGCALHDPLAVGVSLDPSFVTTLDLNMYVQASGEDYGRTIGDPARLNDPTNVTVALTVDKDRYLKTFMNYLTTLFKAH

Sequences:

>Translated_318_residues
MAKRKMILDLDTGIDDAMAIAYAVGAPDVDLIGIISSYGNCLVDQAAINSLQILELLGATDVPVFLGEPHSSTTEHFDVM
PISQQIHGMNGIGDVNLPEPKRAVEKQSGVDFLIDAVHQYGADLTLVPTGPLTNLAEALEKAPDIASTIGNVTLMGGALT
VPGNVSHYAEANINQDAEAANAVFTSTMPLTMVGLDVTLRTLLTKTETQQWRDLKTTAGEKFADIVDYYIAAYDITSPDL
HGCALHDPLAVGVSLDPSFVTTLDLNMYVQASGEDYGRTIGDPARLNDPTNVTVALTVDKDRYLKTFMNYLTTLFKAH
>Mature_317_residues
AKRKMILDLDTGIDDAMAIAYAVGAPDVDLIGIISSYGNCLVDQAAINSLQILELLGATDVPVFLGEPHSSTTEHFDVMP
ISQQIHGMNGIGDVNLPEPKRAVEKQSGVDFLIDAVHQYGADLTLVPTGPLTNLAEALEKAPDIASTIGNVTLMGGALTV
PGNVSHYAEANINQDAEAANAVFTSTMPLTMVGLDVTLRTLLTKTETQQWRDLKTTAGEKFADIVDYYIAAYDITSPDLH
GCALHDPLAVGVSLDPSFVTTLDLNMYVQASGEDYGRTIGDPARLNDPTNVTVALTVDKDRYLKTFMNYLTTLFKAH

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=323, Percent_Identity=36.5325077399381, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=32.3624595469256, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1786213, Length=318, Percent_Identity=30.8176100628931, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI32565474, Length=290, Percent_Identity=28.9655172413793, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565698, Length=207, Percent_Identity=30.9178743961353, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=262, Percent_Identity=26.7175572519084, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583915, Length=192, Percent_Identity=31.25, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641839, Length=187, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24641837, Length=227, Percent_Identity=27.7533039647577, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 34114; Mature: 33983

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRKMILDLDTGIDDAMAIAYAVGAPDVDLIGIISSYGNCLVDQAAINSLQILELLGAT
CCCCCEEEEECCCCCHHHHHHEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
DVPVFLGEPHSSTTEHFDVMPISQQIHGMNGIGDVNLPEPKRAVEKQSGVDFLIDAVHQY
CCCEEECCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
GADLTLVPTGPLTNLAEALEKAPDIASTIGNVTLMGGALTVPGNVSHYAEANINQDAEAA
CCCEEEEECCCHHHHHHHHHHCCHHHHHHCCEEEECCEEECCCCCCHHCCCCCCCCHHHH
NAVFTSTMPLTMVGLDVTLRTLLTKTETQQWRDLKTTAGEKFADIVDYYIAAYDITSPDL
HHEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC
HGCALHDPLAVGVSLDPSFVTTLDLNMYVQASGEDYGRTIGDPARLNDPTNVTVALTVDK
CCEECCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECC
DRYLKTFMNYLTTLFKAH
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKRKMILDLDTGIDDAMAIAYAVGAPDVDLIGIISSYGNCLVDQAAINSLQILELLGAT
CCCCEEEEECCCCCHHHHHHEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
DVPVFLGEPHSSTTEHFDVMPISQQIHGMNGIGDVNLPEPKRAVEKQSGVDFLIDAVHQY
CCCEEECCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHH
GADLTLVPTGPLTNLAEALEKAPDIASTIGNVTLMGGALTVPGNVSHYAEANINQDAEAA
CCCEEEEECCCHHHHHHHHHHCCHHHHHHCCEEEECCEEECCCCCCHHCCCCCCCCHHHH
NAVFTSTMPLTMVGLDVTLRTLLTKTETQQWRDLKTTAGEKFADIVDYYIAAYDITSPDL
HHEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC
HGCALHDPLAVGVSLDPSFVTTLDLNMYVQASGEDYGRTIGDPARLNDPTNVTVALTVDK
CCEECCCCEEEEEECCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECC
DRYLKTFMNYLTTLFKAH
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA