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The map label for this gene is stp [H]

Identifier: 28378378

GI number: 28378378

Start: 1537290

End: 1538993

Strand: Direct

Name: stp [H]

Synonym: lp_1689

Alternate gene names: 28378378

Gene position: 1537290-1538993 (Clockwise)

Preceding gene: 28378376

Following gene: 28378379

Centisome position: 46.47

GC content: 49.71

Gene sequence:

>1704_bases
ATGATGAAAAAATCGACGAAAGTAATTGCAATGATGATGATTGGCATTTTTCTATGTATGCTTGATACGACGGTTATGAA
TATTGCGTTGCCAGCAATTCAGACGGGACTAAATACTGATTTGACGCACCTGTCGTGGGCACTGAATATTTACACCGTCC
TGTTTGCTAGTCTGGCGATTCCACTCGGTCGGTTAGCGGATGTCTGGGGACGTGGGCGGATGTACTTGATTGGACTCGGG
CTCTTTGGTATTGGTTCACTAACTTCTGGATTGAGTCTCAGTGTCGGGCTGCTGATTACTGGCCGAGCGATTCAAAGTAT
TGGTGCGGCCATCGTCTTCCCGGCTAGTATGACGATTGGTATTCAGAGTGTTGCACTCAGCAGGCGTACGGGGGTCATTG
CAAGCTTGGGCGTGATGCAAGGGCTAGCCGCAGCGTTAGGACCAACTATTGGTGGTGCGGTCACTCAATTTTGGGGGTGG
CGGGGAATTTTCCTGATTAACGTGCCTTTAGTTGCCGTCGCGTTGTGTGGTTGCATCGCCTGGCTATCATGGCGGCACCC
TAGTCATGGTCAAGTAAAACTAGACCTCGGTGGGAGTGCCTTAGTGATGACGACCTTATTGGCATTGACGTTGGTCCTGG
TTAAAGGTAATGACTGGGGATGGACGAGTCGCGTAGTCCTGAGCCTGGGCGTCACTAGTGCGCTAACATTGATTGCCTTT
GTCTGGATGGAGGCCCACGTCTCAGCGCCGATGGTCCCATTAGCCCTATTTAAAGACCGACAGTTTAACGGTGCGGCGTT
AGCGACCGTGTTATCAGGGGTCTTTATGGTGGGACTATTAGTCTTGATGCCGAGTTTCTTCACGAAAGTTCAAAGTAAGA
CGGAATTGATGGCGGCGATTATGATTACGCCCGCTTCAATGATGATTTTTATTTTTTCACCAATCAGTGGTTTCTTGTTA
GCAAAGCTTGGTGCGCGGCGCGTGATCTTTAGTGGTAGTCTCGCAATAGCTACGGGTTATGGGGTACTTAGCCTGATGAG
TCCTAGTCAGTACTGGCAGTTTGCATTGGCGGCGTTACTGGTTGGTGCGGGCTACGGCACGATTATTGGGCCAATTACGG
TATTAGCGGCCGGTGATTTTACCGGTGAGTTACTGACAGCGTCACAAAGTGTGATTGGCGTGTTTCGGCAAATTGGCACA
TCGTTGGCCGTAGCCATTTTTGTGTCAGCTTTAGCCGCTAATTTGACAACGGCTAAGACACAGATACGAACGTATGCAAC
GACGACGGTCACTACTTTATCAATTCCTAAGCAAGCCAAACGTGATACGTTACGCGTGGTTGACCACCAATTAGCAACTG
AGCAAACCGCGCAAACGGTCCACGAGCCAATCACGGCGGCTAAGGCCCGCCGGTTGGTTGCTGCCAATTATATGCAAGTG
TTAAAAGCACGGTCATTGCAGTCCGCTCCAGTGCGTATCAAAGCTCAAATTCATCAGCAGGTGGCCCAAAAGGTTCACAG
TCAAGTTAAACACACTAATCATCGAATAAGGACTGCTGCATCAGCGATTAAGGCCAAAACCAAAATTGAGTTAACTGCTG
CGTTTATGCGCCCCTACCAAGTCGCTTGGCCATTCACACTATTGCTAGTGATGACGACCTGGTGTTTTGCGGGACGGGAG
CGGAAAAGTGGCAAGCCGAGTTAA

Upstream 100 bases:

>100_bases
TGTGGGTTCATTGGTTACAACATCTCCTTTTTTGTAACGTAAGTATTTATTTATGACCATTTTACCAGATATACTCCCAT
TAACTTAACGGGAGGCGATT

Downstream 100 bases:

>100_bases
GTCACGTTGATGGGGATGGACTTCAGGCACGTTTGAATAGCTGAGCCGGGAGGGCCGCGAAGGTGATTGCCACCACTGAC
TTTGGAACTTGCCATCGAAC

Product: transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MMKKSTKVIAMMMIGIFLCMLDTTVMNIALPAIQTGLNTDLTHLSWALNIYTVLFASLAIPLGRLADVWGRGRMYLIGLG
LFGIGSLTSGLSLSVGLLITGRAIQSIGAAIVFPASMTIGIQSVALSRRTGVIASLGVMQGLAAALGPTIGGAVTQFWGW
RGIFLINVPLVAVALCGCIAWLSWRHPSHGQVKLDLGGSALVMTTLLALTLVLVKGNDWGWTSRVVLSLGVTSALTLIAF
VWMEAHVSAPMVPLALFKDRQFNGAALATVLSGVFMVGLLVLMPSFFTKVQSKTELMAAIMITPASMMIFIFSPISGFLL
AKLGARRVIFSGSLAIATGYGVLSLMSPSQYWQFALAALLVGAGYGTIIGPITVLAAGDFTGELLTASQSVIGVFRQIGT
SLAVAIFVSALAANLTTAKTQIRTYATTTVTTLSIPKQAKRDTLRVVDHQLATEQTAQTVHEPITAAKARRLVAANYMQV
LKARSLQSAPVRIKAQIHQQVAQKVHSQVKHTNHRIRTAASAIKAKTKIELTAAFMRPYQVAWPFTLLLVMTTWCFAGRE
RKSGKPS

Sequences:

>Translated_567_residues
MMKKSTKVIAMMMIGIFLCMLDTTVMNIALPAIQTGLNTDLTHLSWALNIYTVLFASLAIPLGRLADVWGRGRMYLIGLG
LFGIGSLTSGLSLSVGLLITGRAIQSIGAAIVFPASMTIGIQSVALSRRTGVIASLGVMQGLAAALGPTIGGAVTQFWGW
RGIFLINVPLVAVALCGCIAWLSWRHPSHGQVKLDLGGSALVMTTLLALTLVLVKGNDWGWTSRVVLSLGVTSALTLIAF
VWMEAHVSAPMVPLALFKDRQFNGAALATVLSGVFMVGLLVLMPSFFTKVQSKTELMAAIMITPASMMIFIFSPISGFLL
AKLGARRVIFSGSLAIATGYGVLSLMSPSQYWQFALAALLVGAGYGTIIGPITVLAAGDFTGELLTASQSVIGVFRQIGT
SLAVAIFVSALAANLTTAKTQIRTYATTTVTTLSIPKQAKRDTLRVVDHQLATEQTAQTVHEPITAAKARRLVAANYMQV
LKARSLQSAPVRIKAQIHQQVAQKVHSQVKHTNHRIRTAASAIKAKTKIELTAAFMRPYQVAWPFTLLLVMTTWCFAGRE
RKSGKPS
>Mature_567_residues
MMKKSTKVIAMMMIGIFLCMLDTTVMNIALPAIQTGLNTDLTHLSWALNIYTVLFASLAIPLGRLADVWGRGRMYLIGLG
LFGIGSLTSGLSLSVGLLITGRAIQSIGAAIVFPASMTIGIQSVALSRRTGVIASLGVMQGLAAALGPTIGGAVTQFWGW
RGIFLINVPLVAVALCGCIAWLSWRHPSHGQVKLDLGGSALVMTTLLALTLVLVKGNDWGWTSRVVLSLGVTSALTLIAF
VWMEAHVSAPMVPLALFKDRQFNGAALATVLSGVFMVGLLVLMPSFFTKVQSKTELMAAIMITPASMMIFIFSPISGFLL
AKLGARRVIFSGSLAIATGYGVLSLMSPSQYWQFALAALLVGAGYGTIIGPITVLAAGDFTGELLTASQSVIGVFRQIGT
SLAVAIFVSALAANLTTAKTQIRTYATTTVTTLSIPKQAKRDTLRVVDHQLATEQTAQTVHEPITAAKARRLVAANYMQV
LKARSLQSAPVRIKAQIHQQVAQKVHSQVKHTNHRIRTAASAIKAKTKIELTAAFMRPYQVAWPFTLLLVMTTWCFAGRE
RKSGKPS

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=398, Percent_Identity=27.6381909547739, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789042, Length=394, Percent_Identity=26.9035532994924, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790195, Length=403, Percent_Identity=26.5508684863524, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081983, Length=378, Percent_Identity=25.3968253968254, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=24.330900243309, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=240, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=386, Percent_Identity=26.4248704663212, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=300, Percent_Identity=21.6666666666667, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=33.8345864661654, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 60497; Mature: 60497

Theoretical pI: Translated: 11.32; Mature: 11.32

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKSTKVIAMMMIGIFLCMLDTTVMNIALPAIQTGLNTDLTHLSWALNIYTVLFASLAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
PLGRLADVWGRGRMYLIGLGLFGIGSLTSGLSLSVGLLITGRAIQSIGAAIVFPASMTIG
HHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCEEEHHEEECHHHHHCCCEEEECCHHHHH
IQSVALSRRTGVIASLGVMQGLAAALGPTIGGAVTQFWGWRGIFLINVPLVAVALCGCIA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
WLSWRHPSHGQVKLDLGGSALVMTTLLALTLVLVKGNDWGWTSRVVLSLGVTSALTLIAF
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
VWMEAHVSAPMVPLALFKDRQFNGAALATVLSGVFMVGLLVLMPSFFTKVQSKTELMAAI
HHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MITPASMMIFIFSPISGFLLAKLGARRVIFSGSLAIATGYGVLSLMSPSQYWQFALAALL
HHCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCHHHHCCCHHHHHHHHHHHH
VGAGYGTIIGPITVLAAGDFTGELLTASQSVIGVFRQIGTSLAVAIFVSALAANLTTAKT
HHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIRTYATTTVTTLSIPKQAKRDTLRVVDHQLATEQTAQTVHEPITAAKARRLVAANYMQV
HHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKARSLQSAPVRIKAQIHQQVAQKVHSQVKHTNHRIRTAASAIKAKTKIELTAAFMRPYQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VAWPFTLLLVMTTWCFAGRERKSGKPS
HHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MMKKSTKVIAMMMIGIFLCMLDTTVMNIALPAIQTGLNTDLTHLSWALNIYTVLFASLAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
PLGRLADVWGRGRMYLIGLGLFGIGSLTSGLSLSVGLLITGRAIQSIGAAIVFPASMTIG
HHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCEEEHHEEECHHHHHCCCEEEECCHHHHH
IQSVALSRRTGVIASLGVMQGLAAALGPTIGGAVTQFWGWRGIFLINVPLVAVALCGCIA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
WLSWRHPSHGQVKLDLGGSALVMTTLLALTLVLVKGNDWGWTSRVVLSLGVTSALTLIAF
HHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
VWMEAHVSAPMVPLALFKDRQFNGAALATVLSGVFMVGLLVLMPSFFTKVQSKTELMAAI
HHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MITPASMMIFIFSPISGFLLAKLGARRVIFSGSLAIATGYGVLSLMSPSQYWQFALAALL
HHCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCHHHHCCCHHHHHHHHHHHH
VGAGYGTIIGPITVLAAGDFTGELLTASQSVIGVFRQIGTSLAVAIFVSALAANLTTAKT
HHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIRTYATTTVTTLSIPKQAKRDTLRVVDHQLATEQTAQTVHEPITAAKARRLVAANYMQV
HHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKARSLQSAPVRIKAQIHQQVAQKVHSQVKHTNHRIRTAASAIKAKTKIELTAAFMRPYQ
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VAWPFTLLLVMTTWCFAGRERKSGKPS
HHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]