Click here to switch to the map view.

The map label for this gene is narH [H]

Identifier: 28378214

GI number: 28378214

Start: 1371814

End: 1373373

Strand: Direct

Name: narH [H]

Synonym: lp_1498

Alternate gene names: 28378214

Gene position: 1371814-1373373 (Clockwise)

Preceding gene: 28378213

Following gene: 28378215

Centisome position: 41.47

GC content: 47.24

Gene sequence:

>1560_bases
ATGAAGATTAAAGCACAAATTGGGATGGTCTTAAACCTTGATAAGTGTATTGGTTGCCATACCTGTTCGGTCACTTGTAA
AAATACTTGGACCAATCGTCCTGGGGCAGAATACATGTGGTTTAACAATGTCGAGACCAAACCCGGTGTCGGCTACCCGA
AACGCTGGGAAGATGAAGATCACTATAAAGGTGGTTGGGAGTTGAACAGTAAAGGTAAACTTCAACTCCGAGCAGGTAAT
AAGGTCAATAAGATCGCACTCGGAAAGATTTTTTATCAACCTGATATGCCAGAATTGGACGACTATTATGAACCATGGAC
GTATGATTACCAAACCTTATTCGGACCTGAAAAGGCCCATCAACCAGTTGCACGCGCGACGTCGCAGATTACTGGATTAA
AGATGGATCTTAAGACGGGCCCCAACTGGGATGATGATTTAGCAGGATCACCTGAATATTTCAAAGCAGACCCGAACATG
GAAAAGATAGAGACTGATATCAAAGCGAACTTTGAACAGGCCTTCATGATGTACCTCCCACGTCTATGTGAACATTGCTT
GAACGCACCATGCGTGGCGTCATGTCCTTCTGGGGCGATGTATAAGCGTGATGAAGACGGTATCGTCTTGGTTGATCAAG
AACGGTGCCGCGGCTGGCGTTTTTGTATGACGGGTTGCCCTTATAAGAAGGTCTACTTCAACTGGAAAACCCATAAGGCT
GAAAAATGCACGTTCTGTTATCCACGGATCGAAGAAGGTGAACCGACTGTCTGTGCCGAGACTTGTGTCGGTCGGATCCG
GTATATCGGCGCCATGTTATATGACGCTGATCGGGTCGAAGAAGCGGCCGAAACGCCTGAGGAAGACCAATTATACCAAG
CACAGTTAGATTTGTTCTTGGACCCCAATGATCCAGACATCATTGAACAAGCGTTGGCGGATGGCATCTCTGAAGAAATG
TTGGAAGCTGCTCAGAATTCGCCGATTTACCGGATGGCCGTCGAAGAAAAGATCGCTTTTCCGCTTCATCCTGAATATCG
GACGATGCCCATGGTTTGGTACATCCCACCATTGTCACCAGTCATGAATTATTTTGAGGGCCGCGATTCGATCAAAGATC
CTGAGATGATTTTCCCAGGAATCGATGAGATGCGCATTCCAGTTGATTATTTGGCTAGTTTACTTACGGGTGGCAACGTT
CCGGCAATCAAGGGTGCCCTCTACAAGTTAGCCATGATGCGGCTGTACATGCGGGCTAAGACCGGTGGCAAGGACTTCGA
CGCGGCTAAATTACAACGCGTTGGCTTGACTGAGAACACGGCGACATCACTTTATCGCTTGCTGGCGATTGCCAAGTACG
AAGACCGTTTTGTAATTCCACAAAATCACAAAGAACAAGTGGAAGATGCCCAGAGTGAACAGGGCGGCTTAGGTTATGAC
GAATGTGCTGGCTGCGCGTTAGCACCACAGCATGGCAGTATGTTCAAGAAAGCCGAAGCTGGTGAATCGACCAATCAAAT
TTACGCAGAAAGTTTCTACGGGGGGATTTGGCGTGATTAA

Upstream 100 bases:

>100_bases
GGTTATGGTCAGTTGAGTTATGGTTTCAATTATTATGGCCCAATCGGTAATCAACGGGACCTGTACGTGAACGTACGGAA
GTTAAAGAAGGTGAACTGGA

Downstream 100 bases:

>100_bases
TTTTAAACAATTAACAACGATGCAACCCGCGCTAGTTACTTTGTCGCGGTTGATCGACTATCCTGATGAGATGACGTTCG
CTACGGCTACCCGTCAGAAT

Product: nitrate reductase, beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKIKAQIGMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDEDHYKGGWELNSKGKLQLRAGN
KVNKIALGKIFYQPDMPELDDYYEPWTYDYQTLFGPEKAHQPVARATSQITGLKMDLKTGPNWDDDLAGSPEYFKADPNM
EKIETDIKANFEQAFMMYLPRLCEHCLNAPCVASCPSGAMYKRDEDGIVLVDQERCRGWRFCMTGCPYKKVYFNWKTHKA
EKCTFCYPRIEEGEPTVCAETCVGRIRYIGAMLYDADRVEEAAETPEEDQLYQAQLDLFLDPNDPDIIEQALADGISEEM
LEAAQNSPIYRMAVEEKIAFPLHPEYRTMPMVWYIPPLSPVMNYFEGRDSIKDPEMIFPGIDEMRIPVDYLASLLTGGNV
PAIKGALYKLAMMRLYMRAKTGGKDFDAAKLQRVGLTENTATSLYRLLAIAKYEDRFVIPQNHKEQVEDAQSEQGGLGYD
ECAGCALAPQHGSMFKKAEAGESTNQIYAESFYGGIWRD

Sequences:

>Translated_519_residues
MKIKAQIGMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDEDHYKGGWELNSKGKLQLRAGN
KVNKIALGKIFYQPDMPELDDYYEPWTYDYQTLFGPEKAHQPVARATSQITGLKMDLKTGPNWDDDLAGSPEYFKADPNM
EKIETDIKANFEQAFMMYLPRLCEHCLNAPCVASCPSGAMYKRDEDGIVLVDQERCRGWRFCMTGCPYKKVYFNWKTHKA
EKCTFCYPRIEEGEPTVCAETCVGRIRYIGAMLYDADRVEEAAETPEEDQLYQAQLDLFLDPNDPDIIEQALADGISEEM
LEAAQNSPIYRMAVEEKIAFPLHPEYRTMPMVWYIPPLSPVMNYFEGRDSIKDPEMIFPGIDEMRIPVDYLASLLTGGNV
PAIKGALYKLAMMRLYMRAKTGGKDFDAAKLQRVGLTENTATSLYRLLAIAKYEDRFVIPQNHKEQVEDAQSEQGGLGYD
ECAGCALAPQHGSMFKKAEAGESTNQIYAESFYGGIWRD
>Mature_519_residues
MKIKAQIGMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDEDHYKGGWELNSKGKLQLRAGN
KVNKIALGKIFYQPDMPELDDYYEPWTYDYQTLFGPEKAHQPVARATSQITGLKMDLKTGPNWDDDLAGSPEYFKADPNM
EKIETDIKANFEQAFMMYLPRLCEHCLNAPCVASCPSGAMYKRDEDGIVLVDQERCRGWRFCMTGCPYKKVYFNWKTHKA
EKCTFCYPRIEEGEPTVCAETCVGRIRYIGAMLYDADRVEEAAETPEEDQLYQAQLDLFLDPNDPDIIEQALADGISEEM
LEAAQNSPIYRMAVEEKIAFPLHPEYRTMPMVWYIPPLSPVMNYFEGRDSIKDPEMIFPGIDEMRIPVDYLASLLTGGNV
PAIKGALYKLAMMRLYMRAKTGGKDFDAAKLQRVGLTENTATSLYRLLAIAKYEDRFVIPQNHKEQVEDAQSEQGGLGYD
ECAGCALAPQHGSMFKKAEAGESTNQIYAESFYGGIWRD

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=496, Percent_Identity=58.0645161290323, Blast_Score=609, Evalue=1e-175,
Organism=Escherichia coli, GI1787740, Length=500, Percent_Identity=55.2, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=40.5940594059406, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=40.5940594059406, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI226510944, Length=120, Percent_Identity=35, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=28.3687943262411, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1789370, Length=104, Percent_Identity=32.6923076923077, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59013; Mature: 59013

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQIGMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDED
CEEEEEECEEEEHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
HYKGGWELNSKGKLQLRAGNKVNKIALGKIFYQPDMPELDDYYEPWTYDYQTLFGPEKAH
CCCCCEEECCCCEEEEECCCCCCEEEEEEEEECCCCCCCHHCCCCCCCCHHHHCCCHHHC
QPVARATSQITGLKMDLKTGPNWDDDLAGSPEYFKADPNMEKIETDIKANFEQAFMMYLP
CHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNAPCVASCPSGAMYKRDEDGIVLVDQERCRGWRFCMTGCPYKKVYFNWKTHKA
HHHHHHCCCCCCCCCCCCCCEEECCCCEEEEEHHHCCCEEEECCCCCCEEEEEEEECCCC
EKCTFCYPRIEEGEPTVCAETCVGRIRYIGAMLYDADRVEEAAETPEEDQLYQAQLDLFL
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHEEEEEEEEE
DPNDPDIIEQALADGISEEMLEAAQNSPIYRMAVEEKIAFPLHPEYRTMPMVWYIPPLSP
CCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCEEEEECCHHH
VMNYFEGRDSIKDPEMIFPGIDEMRIPVDYLASLLTGGNVPAIKGALYKLAMMRLYMRAK
HHHHHCCCCCCCCCCEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
TGGKDFDAAKLQRVGLTENTATSLYRLLAIAKYEDRFVIPQNHKEQVEDAQSEQGGLGYD
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCHH
ECAGCALAPQHGSMFKKAEAGESTNQIYAESFYGGIWRD
HHCCCEECCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIKAQIGMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDED
CEEEEEECEEEEHHHHCCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
HYKGGWELNSKGKLQLRAGNKVNKIALGKIFYQPDMPELDDYYEPWTYDYQTLFGPEKAH
CCCCCEEECCCCEEEEECCCCCCEEEEEEEEECCCCCCCHHCCCCCCCCHHHHCCCHHHC
QPVARATSQITGLKMDLKTGPNWDDDLAGSPEYFKADPNMEKIETDIKANFEQAFMMYLP
CHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNAPCVASCPSGAMYKRDEDGIVLVDQERCRGWRFCMTGCPYKKVYFNWKTHKA
HHHHHHCCCCCCCCCCCCCCEEECCCCEEEEEHHHCCCEEEECCCCCCEEEEEEEECCCC
EKCTFCYPRIEEGEPTVCAETCVGRIRYIGAMLYDADRVEEAAETPEEDQLYQAQLDLFL
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHEEEEEEEEE
DPNDPDIIEQALADGISEEMLEAAQNSPIYRMAVEEKIAFPLHPEYRTMPMVWYIPPLSP
CCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCCCCEEEEECCHHH
VMNYFEGRDSIKDPEMIFPGIDEMRIPVDYLASLLTGGNVPAIKGALYKLAMMRLYMRAK
HHHHHCCCCCCCCCCEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
TGGKDFDAAKLQRVGLTENTATSLYRLLAIAKYEDRFVIPQNHKEQVEDAQSEQGGLGYD
CCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCCCHH
ECAGCALAPQHGSMFKKAEAGESTNQIYAESFYGGIWRD
HHCCCEECCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]