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The map label for this gene is moaE [H]

Identifier: 28378194

GI number: 28378194

Start: 1354148

End: 1354549

Strand: Direct

Name: moaE [H]

Synonym: lp_1478

Alternate gene names: 28378194

Gene position: 1354148-1354549 (Clockwise)

Preceding gene: 28378190

Following gene: 28378195

Centisome position: 40.93

GC content: 49.75

Gene sequence:

>402_bases
ATGGCGATGATCAAATTGTCTGCGACGCCATTGGATGTGAATGCGTTGTATCAAGTATTGAAGGCACCGGAATATGGCGG
CATTGTCACGTTTGTAGGCACTGTTCGCCAGTGGACGGGCCCAATCGAAACGCAATCAATCGATTATTCGGCGTATGCGG
AGATGGCCATCAGTCAACTTAACAAATTAGCAGCGCCCATTGAAGCTAAAGGCGCCCGCGTGGTGATCGTGCACCGAATT
GGGCATCTGGATCTGATGGATGAAGCGGTCTTTGTTGGTGTCGCGGCAGCGCACCGTGCTGAGGCCTTCGAATGGTGCCA
ATACTTAATTGATACACTGAAAAAGGAAGTCCCGATTTGGAAGAAAGAATTTGATACCGACAAGGTTCGTTGGGGGGATT
AA

Upstream 100 bases:

>100_bases
CACGAATTGACCATTATTAGGGATTTGACTAATTAAATTTAAAAGCAAGCTCTGACATAATAGAAGGGTAGGACGAATCG
GTCAAAAGGAGGGGTGCGCC

Downstream 100 bases:

>100_bases
AGATGGAACTAACGATTAAGTTGTTTGCAATGCTAGCGGAACAAATCGGGCCAACGGTCACGGTGACCGTATCAACGCCG
GCGACCGCGGCCATGGTCAA

Product: molybdopterin biosynthesis protein, E chain

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E 1; Molybdopterin-converting factor large subunit 1; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MAMIKLSATPLDVNALYQVLKAPEYGGIVTFVGTVRQWTGPIETQSIDYSAYAEMAISQLNKLAAPIEAKGARVVIVHRI
GHLDLMDEAVFVGVAAAHRAEAFEWCQYLIDTLKKEVPIWKKEFDTDKVRWGD

Sequences:

>Translated_133_residues
MAMIKLSATPLDVNALYQVLKAPEYGGIVTFVGTVRQWTGPIETQSIDYSAYAEMAISQLNKLAAPIEAKGARVVIVHRI
GHLDLMDEAVFVGVAAAHRAEAFEWCQYLIDTLKKEVPIWKKEFDTDKVRWGD
>Mature_132_residues
AMIKLSATPLDVNALYQVLKAPEYGGIVTFVGTVRQWTGPIETQSIDYSAYAEMAISQLNKLAAPIEAKGARVVIVHRIG
HLDLMDEAVFVGVAAAHRAEAFEWCQYLIDTLKKEVPIWKKEFDTDKVRWGD

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=131, Percent_Identity=34.3511450381679, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787003, Length=133, Percent_Identity=36.0902255639098, Blast_Score=74, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509279, Length=123, Percent_Identity=30.8943089430894, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356259, Length=131, Percent_Identity=35.1145038167939, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 14851; Mature: 14720

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMIKLSATPLDVNALYQVLKAPEYGGIVTFVGTVRQWTGPIETQSIDYSAYAEMAISQL
CCEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
NKLAAPIEAKGARVVIVHRIGHLDLMDEAVFVGVAAAHRAEAFEWCQYLIDTLKKEVPIW
HHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KKEFDTDKVRWGD
HHCCCCCCCCCCC
>Mature Secondary Structure 
AMIKLSATPLDVNALYQVLKAPEYGGIVTFVGTVRQWTGPIETQSIDYSAYAEMAISQL
CEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
NKLAAPIEAKGARVVIVHRIGHLDLMDEAVFVGVAAAHRAEAFEWCQYLIDTLKKEVPIW
HHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KKEFDTDKVRWGD
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]