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The map label for this gene is ptsI [H]

Identifier: 28378036

GI number: 28378036

Start: 1157416

End: 1159146

Strand: Direct

Name: ptsI [H]

Synonym: lp_1274

Alternate gene names: 28378036

Gene position: 1157416-1159146 (Clockwise)

Preceding gene: 28378035

Following gene: 28378037

Centisome position: 34.99

GC content: 43.85

Gene sequence:

>1731_bases
ATGGCTGAAAAGGTACTAAAAGGAATTGCGGCCAGTGATGGTGTTGCAATTGCTAAGGCCTATATGCTAGTTGATCCAGA
TTTATCATTCGAAAAGACAACAGTTTCCGATACGGATGCTGAAGTTGCACGGTTACATGACGCTTTCGATGCTTCCAAGG
CTGAACTAAAGGTCATTAAAGACAAAGCGGTTGAAAACTTGGGTGAAGAAGAAGCAGAAGTTTTTGAAGCTCATATCACC
ATTTTGTCTGACCCAGAAATGCTCGGACAAATTGAAGGTAAGATCAAGGATGATAAAGTCAATGCTGAAGAAGCTTTGAA
GGAAGTTACTGATACTTTCATTTCAATGTTTGAAGCGATGACGGATAATGCATATATGCAAGAACGAGCCGGTGATATTC
GCGACGTAACGAAGCGGGTCTTAAGCCACTTGTTAGGGGTAACCTTACCAAGTCCAGCTTTAATTGACTCCGAAGTAATC
GTGGTTGCCCACGATTTAACACCTAGTGATACAGCTCAACTTGATCGTAAGTATGTTAAGGGCTTCATTACTGACATTGG
TGGTCGGACTAGTCATTCAGCCATCATGTCACGGACGCTTGAAATTCCAGCGGTCGTTGGATCTGAATCTGCCACGACTG
ATATCAAAGAAGGCGATACGGTCGTCTTGGATGGTATCAACGGTGACGCTTTAGTAGCCCCAACGGATGCTGAAGTTGCT
GATTACCAACAAAAGGCTAAAGACTTTGTTGCGCAAAAGGCTGAATGGGCTAAGTTGAAGAACGAGGCCACCGTTTCTAA
GGACGGCAAGCACTTTGAATTAGCTTCTAACATCGGGACACCTGACGATATGGATGGGGTTTTGGATGCTGGCTCTGAAG
CCATTGGATTATTCCGTTCAGAATTCTTGTACATGAACAGTTCAGAATTACCTGACGAAGATACCCAATTCGAAGCTTAC
AAGAAGGTTGTCGAAGGGATGAACGGCAAACCCGTTATTGTTCGGACCATGGATATCGGTGGTGACAAGGAATTACCTTA
CTTGCCATTACCTGATGAAATGAACCCATTCTTGGGTTACCGGGCTATTCGGATTTCCCTCGATCGTGATGACATCTTCC
GGACCCAGTTACGTGCGTTATTACGTGCGTCACATTACGGTCAATTACGGATTATGTTCCCAATGATTGCAACGCTTGAC
GAATTCCGTAAAGCCAAAGCTATCTTTGAGGAAGAAAAAGCCAAGTTGGTTGCAGCTGGTACGCCTGTTGCTGACGACAT
CAAGCTTGGTATCATGATTGAAATCCCAGCGGCAGCTATTTTAGCTGACCAGTTTGCTAAGGAAGTCGACTTCTTTAGTA
TTGGGACTAACGACTTGATTCAATACTCATTTGCTGCTGATCGTGGTAACGAACAAGTGTCATACTTGTATCAACCATAC
AACCCATCGTTGTTGCGTTTGATCAAGCATGTTATTGACGCTGCTCACGCGAACGGCCGCTTCACGGGTATGTGTGGTGA
AGTTGCTGGGGATCAGATCGCGGTGCCATTATTGATGGGCTTAGGTTTGGATGAATTCTCAATGAGTTCAACTTCCGTGC
TTAAGACACGTTCATTAATGAAGAAACTTGATACTAAGGAAATGGCTAAATTAGCTGATAAGGCACTTAACGAGTGTGTT
ACTAACGAAGAAGTCAAAGAATTAGTTGAAAAGAACGTCTTCGGTAAATAA

Upstream 100 bases:

>100_bases
GTAAAGACGCTGACGTTACCATTTCTGCTGAAGGTGCCGACGAAGCCGACGCTATCGCAGCTTTAACTGAAACGATGAAG
AAAGAAGGATTATCTGAATA

Downstream 100 bases:

>100_bases
ACAATCAAACTAAGAGCAAATCTGTGGATTTGCTCTTTTTTTGTGGGTAAATGCGGGTCATTAACCAGTTGGTACTAGCC
CCGGGTTTAGCACGACGGGG

Product: phosphoenolpyruvate--protein phosphatase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MAEKVLKGIAASDGVAIAKAYMLVDPDLSFEKTTVSDTDAEVARLHDAFDASKAELKVIKDKAVENLGEEEAEVFEAHIT
ILSDPEMLGQIEGKIKDDKVNAEEALKEVTDTFISMFEAMTDNAYMQERAGDIRDVTKRVLSHLLGVTLPSPALIDSEVI
VVAHDLTPSDTAQLDRKYVKGFITDIGGRTSHSAIMSRTLEIPAVVGSESATTDIKEGDTVVLDGINGDALVAPTDAEVA
DYQQKAKDFVAQKAEWAKLKNEATVSKDGKHFELASNIGTPDDMDGVLDAGSEAIGLFRSEFLYMNSSELPDEDTQFEAY
KKVVEGMNGKPVIVRTMDIGGDKELPYLPLPDEMNPFLGYRAIRISLDRDDIFRTQLRALLRASHYGQLRIMFPMIATLD
EFRKAKAIFEEEKAKLVAAGTPVADDIKLGIMIEIPAAAILADQFAKEVDFFSIGTNDLIQYSFAADRGNEQVSYLYQPY
NPSLLRLIKHVIDAAHANGRFTGMCGEVAGDQIAVPLLMGLGLDEFSMSSTSVLKTRSLMKKLDTKEMAKLADKALNECV
TNEEVKELVEKNVFGK

Sequences:

>Translated_576_residues
MAEKVLKGIAASDGVAIAKAYMLVDPDLSFEKTTVSDTDAEVARLHDAFDASKAELKVIKDKAVENLGEEEAEVFEAHIT
ILSDPEMLGQIEGKIKDDKVNAEEALKEVTDTFISMFEAMTDNAYMQERAGDIRDVTKRVLSHLLGVTLPSPALIDSEVI
VVAHDLTPSDTAQLDRKYVKGFITDIGGRTSHSAIMSRTLEIPAVVGSESATTDIKEGDTVVLDGINGDALVAPTDAEVA
DYQQKAKDFVAQKAEWAKLKNEATVSKDGKHFELASNIGTPDDMDGVLDAGSEAIGLFRSEFLYMNSSELPDEDTQFEAY
KKVVEGMNGKPVIVRTMDIGGDKELPYLPLPDEMNPFLGYRAIRISLDRDDIFRTQLRALLRASHYGQLRIMFPMIATLD
EFRKAKAIFEEEKAKLVAAGTPVADDIKLGIMIEIPAAAILADQFAKEVDFFSIGTNDLIQYSFAADRGNEQVSYLYQPY
NPSLLRLIKHVIDAAHANGRFTGMCGEVAGDQIAVPLLMGLGLDEFSMSSTSVLKTRSLMKKLDTKEMAKLADKALNECV
TNEEVKELVEKNVFGK
>Mature_575_residues
AEKVLKGIAASDGVAIAKAYMLVDPDLSFEKTTVSDTDAEVARLHDAFDASKAELKVIKDKAVENLGEEEAEVFEAHITI
LSDPEMLGQIEGKIKDDKVNAEEALKEVTDTFISMFEAMTDNAYMQERAGDIRDVTKRVLSHLLGVTLPSPALIDSEVIV
VAHDLTPSDTAQLDRKYVKGFITDIGGRTSHSAIMSRTLEIPAVVGSESATTDIKEGDTVVLDGINGDALVAPTDAEVAD
YQQKAKDFVAQKAEWAKLKNEATVSKDGKHFELASNIGTPDDMDGVLDAGSEAIGLFRSEFLYMNSSELPDEDTQFEAYK
KVVEGMNGKPVIVRTMDIGGDKELPYLPLPDEMNPFLGYRAIRISLDRDDIFRTQLRALLRASHYGQLRIMFPMIATLDE
FRKAKAIFEEEKAKLVAAGTPVADDIKLGIMIEIPAAAILADQFAKEVDFFSIGTNDLIQYSFAADRGNEQVSYLYQPYN
PSLLRLIKHVIDAAHANGRFTGMCGEVAGDQIAVPLLMGLGLDEFSMSSTSVLKTRSLMKKLDTKEMAKLADKALNECVT
NEEVKELVEKNVFGK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=562, Percent_Identity=48.3985765124555, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1788726, Length=533, Percent_Identity=37.7110694183865, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI48994992, Length=517, Percent_Identity=36.7504835589942, Blast_Score=342, Evalue=5e-95,
Organism=Escherichia coli, GI1789193, Length=584, Percent_Identity=32.3630136986301, Blast_Score=308, Evalue=8e-85,
Organism=Escherichia coli, GI1787994, Length=425, Percent_Identity=28.2352941176471, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI226510935, Length=181, Percent_Identity=29.8342541436464, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63112; Mature: 62980

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKVLKGIAASDGVAIAKAYMLVDPDLSFEKTTVSDTDAEVARLHDAFDASKAELKVIK
CHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
DKAVENLGEEEAEVFEAHITILSDPEMLGQIEGKIKDDKVNAEEALKEVTDTFISMFEAM
HHHHHHCCHHHHHHHHHHEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TDNAYMQERAGDIRDVTKRVLSHLLGVTLPSPALIDSEVIVVAHDLTPSDTAQLDRKYVK
HCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHHHH
GFITDIGGRTSHSAIMSRTLEIPAVVGSESATTDIKEGDTVVLDGINGDALVAPTDAEVA
HHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCCCEEECCCCCHHH
DYQQKAKDFVAQKAEWAKLKNEATVSKDGKHFELASNIGTPDDMDGVLDAGSEAIGLFRS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCHHHHHHHHH
EFLYMNSSELPDEDTQFEAYKKVVEGMNGKPVIVRTMDIGGDKELPYLPLPDEMNPFLGY
HHEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHCE
RAIRISLDRDDIFRTQLRALLRASHYGQLRIMFPMIATLDEFRKAKAIFEEEKAKLVAAG
EEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCEEEECC
TPVADDIKLGIMIEIPAAAILADQFAKEVDFFSIGTNDLIQYSFAADRGNEQVSYLYQPY
CCCCCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHEEEEECCCCCCCCEEEEECCC
NPSLLRLIKHVIDAAHANGRFTGMCGEVAGDQIAVPLLMGLGLDEFSMSSTSVLKTRSLM
CHHHHHHHHHHHHHHCCCCCEEEECHHHCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
KKLDTKEMAKLADKALNECVTNEEVKELVEKNVFGK
HHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEKVLKGIAASDGVAIAKAYMLVDPDLSFEKTTVSDTDAEVARLHDAFDASKAELKVIK
HHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
DKAVENLGEEEAEVFEAHITILSDPEMLGQIEGKIKDDKVNAEEALKEVTDTFISMFEAM
HHHHHHCCHHHHHHHHHHEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TDNAYMQERAGDIRDVTKRVLSHLLGVTLPSPALIDSEVIVVAHDLTPSDTAQLDRKYVK
HCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHHHH
GFITDIGGRTSHSAIMSRTLEIPAVVGSESATTDIKEGDTVVLDGINGDALVAPTDAEVA
HHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEEECCCCCEEECCCCCHHH
DYQQKAKDFVAQKAEWAKLKNEATVSKDGKHFELASNIGTPDDMDGVLDAGSEAIGLFRS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCHHHHHHHHH
EFLYMNSSELPDEDTQFEAYKKVVEGMNGKPVIVRTMDIGGDKELPYLPLPDEMNPFLGY
HHEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHCE
RAIRISLDRDDIFRTQLRALLRASHYGQLRIMFPMIATLDEFRKAKAIFEEEKAKLVAAG
EEEEEECCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCEEEECC
TPVADDIKLGIMIEIPAAAILADQFAKEVDFFSIGTNDLIQYSFAADRGNEQVSYLYQPY
CCCCCCEEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHEEEEECCCCCCCCEEEEECCC
NPSLLRLIKHVIDAAHANGRFTGMCGEVAGDQIAVPLLMGLGLDEFSMSSTSVLKTRSLM
CHHHHHHHHHHHHHHCCCCCEEEECHHHCCCHHHHHHHHHCCCCHHCCCHHHHHHHHHHH
KKLDTKEMAKLADKALNECVTNEEVKELVEKNVFGK
HHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9172326 [H]