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The map label for this gene is prfC

Identifier: 28378021

GI number: 28378021

Start: 1138710

End: 1140242

Strand: Direct

Name: prfC

Synonym: lp_1255

Alternate gene names: 28378021

Gene position: 1138710-1140242 (Clockwise)

Preceding gene: 28378016

Following gene: 28378022

Centisome position: 34.42

GC content: 47.16

Gene sequence:

>1533_bases
ATGAATAAGACAGAATTAACCGCCGCGGTCAATACCCGGCGTACCTTTGCGATTATTTCTCACCCGGATGCCGGGAAAAC
CACCATTACGGAACAAATGTTATTGTTTGGTGGCGTTGTTCGTCAGGCCGGAACGGTTAAAGCCCGTAAAAGTGGGAACT
TTGCCAAGTCTGACTGGATGGAAATTGAAAAGAAGCGGGGAATCTCAGTAACGAGTTCCGTGATGCAATTCGATTACGAT
GGCAAACGGATCAACATCTTGGACACGCCCGGACATGAGGATTTCTCGGAAGACACTTATCGGACTTTGATGGCGGTCGA
TTCCGCTGTCATGGTCATTGACTCGGCTAAGGGGATCGAGCCACAAACGAAGAAGTTATTCCAAATTTGTAAGATGCGGG
GCATTCCGATCTTTACGTTCATGAACAAGTTAGACCGTGATGGTCGCGAACCATTGGACTTGTTGAACGAAGTCGAAGAA
GTCCTCGGAATCGAAACTTATCCAATGAACTGGCCAATGGGTATGGGTAAGGGCTTAAAGGGCTTATACGATCGTTACAA
TCACCGTGTGGAACTTTATCATAATGAAGCTCATGGCGAAACGTTCTTACCACTAGACGAGAACGGTGAGCTTGATCCTA
GTAATGAATTGACCAAGGACAGTATTTATCGTGACACGTTGGATGAAGTCGAATTGATCAACGAAGCTGGTAATGATTTT
GACGAAGCTAAAGTAATGGCGGGAGACATGACGCCAGTATTTTTTGGGTCTGCCCTCACCAATTTCGGGGTCGAAACGTT
CTTGAAGACGTATCTGCAGTATGCGCCTAAACCCGCTGCTCATAAGACTCGTGATGACCAAGTCGTTCAGCCAACCGACG
ATGAATTTGCCGGTTTTGTGTTCAAGATCCAAGCCAACATGAACCCGAACCATCGTGATCGGATCGCCTTTGTCCGCATT
TGTTCTGGCGAATTTGACCGGGGGATGGACGTGGTCTTGCAGCGGACTGGTAAGAAGATGCGCTTAAACAACTCTACGCA
ATTCATGGCCGATACCCGTGAAACGGTGGAAACTGCCGTTGCTGGCGATATCGTCGGCCTCTACGACACGGGTAACTTCC
AAATTGGAGATACGATTTACGCTGGCAAAAATGCGGTTCAATTCGAAAAGTTACCGCAATTTACGCCGGAGCTCTTCATG
CGCGTTACAGCCAAGAACGTCATGAAACAGAAGTCCTTCCATAAAGGTATTGAACAACTGGTTCAAGAAGGTGCCGTTCA
GTTGTACACTTCATATTCAACGGGTGACTATATTCTCGGTGCAGTCGGTCAACTTCAATTTGAAGTCTTCAAATTCCGGA
TGCAAAATGAATATAATTCGGAAGTTGTCATGGAACCAATGGGTAGCAAGACTGCCCGCTGGATCGATCCCGAACAATTG
GATGAAAAGATGTCTAGTTCGCGGAACCTACTAGTCAAGGACCGCTACGGTGCACCGCTCTTCTTATTCGAAAACGCCTT
TGCCGAACGCTAA

Upstream 100 bases:

>100_bases
TGGTCAAATATCAGTAAAATATGTAAGGCTACGTTTTTTTACGGTGGGACGGTACCACCGTGCACGTTGAATATACAGGT
TATGATGAAAGGAATTTTTA

Downstream 100 bases:

>100_bases
TTTGCGGATAAGTATCCGGATGTCAAGCTGACCTCGAAACTCTAGGATTTATATTGTGGTTTACACTTAGCTGACCAATC
TAGTTAGATTACTGTTCGTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MNKTELTAAVNTRRTFAIISHPDAGKTTITEQMLLFGGVVRQAGTVKARKSGNFAKSDWMEIEKKRGISVTSSVMQFDYD
GKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIEPQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLLNEVEE
VLGIETYPMNWPMGMGKGLKGLYDRYNHRVELYHNEAHGETFLPLDENGELDPSNELTKDSIYRDTLDEVELINEAGNDF
DEAKVMAGDMTPVFFGSALTNFGVETFLKTYLQYAPKPAAHKTRDDQVVQPTDDEFAGFVFKIQANMNPNHRDRIAFVRI
CSGEFDRGMDVVLQRTGKKMRLNNSTQFMADTRETVETAVAGDIVGLYDTGNFQIGDTIYAGKNAVQFEKLPQFTPELFM
RVTAKNVMKQKSFHKGIEQLVQEGAVQLYTSYSTGDYILGAVGQLQFEVFKFRMQNEYNSEVVMEPMGSKTARWIDPEQL
DEKMSSSRNLLVKDRYGAPLFLFENAFAER

Sequences:

>Translated_510_residues
MNKTELTAAVNTRRTFAIISHPDAGKTTITEQMLLFGGVVRQAGTVKARKSGNFAKSDWMEIEKKRGISVTSSVMQFDYD
GKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIEPQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLLNEVEE
VLGIETYPMNWPMGMGKGLKGLYDRYNHRVELYHNEAHGETFLPLDENGELDPSNELTKDSIYRDTLDEVELINEAGNDF
DEAKVMAGDMTPVFFGSALTNFGVETFLKTYLQYAPKPAAHKTRDDQVVQPTDDEFAGFVFKIQANMNPNHRDRIAFVRI
CSGEFDRGMDVVLQRTGKKMRLNNSTQFMADTRETVETAVAGDIVGLYDTGNFQIGDTIYAGKNAVQFEKLPQFTPELFM
RVTAKNVMKQKSFHKGIEQLVQEGAVQLYTSYSTGDYILGAVGQLQFEVFKFRMQNEYNSEVVMEPMGSKTARWIDPEQL
DEKMSSSRNLLVKDRYGAPLFLFENAFAER
>Mature_510_residues
MNKTELTAAVNTRRTFAIISHPDAGKTTITEQMLLFGGVVRQAGTVKARKSGNFAKSDWMEIEKKRGISVTSSVMQFDYD
GKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIEPQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLLNEVEE
VLGIETYPMNWPMGMGKGLKGLYDRYNHRVELYHNEAHGETFLPLDENGELDPSNELTKDSIYRDTLDEVELINEAGNDF
DEAKVMAGDMTPVFFGSALTNFGVETFLKTYLQYAPKPAAHKTRDDQVVQPTDDEFAGFVFKIQANMNPNHRDRIAFVRI
CSGEFDRGMDVVLQRTGKKMRLNNSTQFMADTRETVETAVAGDIVGLYDTGNFQIGDTIYAGKNAVQFEKLPQFTPELFM
RVTAKNVMKQKSFHKGIEQLVQEGAVQLYTSYSTGDYILGAVGQLQFEVFKFRMQNEYNSEVVMEPMGSKTARWIDPEQL
DEKMSSSRNLLVKDRYGAPLFLFENAFAER

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=28.659793814433, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI19923640, Length=509, Percent_Identity=26.7190569744597, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=29.9492385786802, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI25306287, Length=290, Percent_Identity=32.0689655172414, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI157426893, Length=161, Percent_Identity=29.8136645962733, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=29.1970802919708, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.1879194630872, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=29.3103448275862, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=29.3103448275862, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=29.3103448275862, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790835, Length=472, Percent_Identity=50.635593220339, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789738, Length=487, Percent_Identity=27.7207392197125, Blast_Score=178, Evalue=6e-46,
Organism=Escherichia coli, GI48994988, Length=172, Percent_Identity=31.3953488372093, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=179, Percent_Identity=29.0502793296089, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789737, Length=132, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790412, Length=132, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=475, Percent_Identity=28.2105263157895, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=172, Percent_Identity=35.4651162790698, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17506493, Length=539, Percent_Identity=23.3766233766234, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=30.3703703703704, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.8270676691729, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.8270676691729, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=26.3485477178423, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=507, Percent_Identity=26.4299802761341, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324707, Length=547, Percent_Identity=21.9378427787934, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320593, Length=547, Percent_Identity=21.9378427787934, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=174, Percent_Identity=28.735632183908, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=27.7083333333333, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=26.5346534653465, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI78706572, Length=156, Percent_Identity=30.1282051282051, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=539, Percent_Identity=22.0779220779221, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585711, Length=539, Percent_Identity=22.0779220779221, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585713, Length=539, Percent_Identity=22.0779220779221, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574573, Length=171, Percent_Identity=32.7485380116959, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_LACPL (Q88XF3)

Other databases:

- EMBL:   AL935255
- RefSeq:   NP_784913.1
- ProteinModelPortal:   Q88XF3
- SMR:   Q88XF3
- GeneID:   1062966
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1255
- NMPDR:   fig|220668.1.peg.1047
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   LPLA220668:LP_1255-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 57769; Mature: 57769

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTELTAAVNTRRTFAIISHPDAGKTTITEQMLLFGGVVRQAGTVKARKSGNFAKSDWM
CCCHHEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHH
EIEKKRGISVTSSVMQFDYDGKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIE
HHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
PQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLLNEVEEVLGIETYPMNWPMGMGKGLK
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
GLYDRYNHRVELYHNEAHGETFLPLDENGELDPSNELTKDSIYRDTLDEVELINEAGNDF
HHHHHHCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DEAKVMAGDMTPVFFGSALTNFGVETFLKTYLQYAPKPAAHKTRDDQVVQPTDDEFAGFV
HHHHEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
FKIQANMNPNHRDRIAFVRICSGEFDRGMDVVLQRTGKKMRLNNSTQFMADTRETVETAV
EEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
AGDIVGLYDTGNFQIGDTIYAGKNAVQFEKLPQFTPELFMRVTAKNVMKQKSFHKGIEQL
HCCEEEEEECCCEEECCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQEGAVQLYTSYSTGDYILGAVGQLQFEVFKFRMQNEYNSEVVMEPMGSKTARWIDPEQL
HHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHH
DEKMSSSRNLLVKDRYGAPLFLFENAFAER
HHHHHCCCCEEEEECCCCCEEEECHHHCCC
>Mature Secondary Structure
MNKTELTAAVNTRRTFAIISHPDAGKTTITEQMLLFGGVVRQAGTVKARKSGNFAKSDWM
CCCHHEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHH
EIEKKRGISVTSSVMQFDYDGKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIE
HHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
PQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLLNEVEEVLGIETYPMNWPMGMGKGLK
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
GLYDRYNHRVELYHNEAHGETFLPLDENGELDPSNELTKDSIYRDTLDEVELINEAGNDF
HHHHHHCCEEEEEEECCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
DEAKVMAGDMTPVFFGSALTNFGVETFLKTYLQYAPKPAAHKTRDDQVVQPTDDEFAGFV
HHHHEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
FKIQANMNPNHRDRIAFVRICSGEFDRGMDVVLQRTGKKMRLNNSTQFMADTRETVETAV
EEEEECCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHH
AGDIVGLYDTGNFQIGDTIYAGKNAVQFEKLPQFTPELFMRVTAKNVMKQKSFHKGIEQL
HCCEEEEEECCCEEECCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VQEGAVQLYTSYSTGDYILGAVGQLQFEVFKFRMQNEYNSEVVMEPMGSKTARWIDPEQL
HHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHH
DEKMSSSRNLLVKDRYGAPLFLFENAFAER
HHHHHCCCCEEEEECCCCCEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566