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The map label for this gene is apbE1 [H]

Identifier: 28377866

GI number: 28377866

Start: 974330

End: 975442

Strand: Direct

Name: apbE1 [H]

Synonym: lp_1072

Alternate gene names: 28377866

Gene position: 974330-975442 (Clockwise)

Preceding gene: 28377865

Following gene: 28377867

Centisome position: 29.45

GC content: 43.58

Gene sequence:

>1113_bases
ATGAAGCTAAAAAAATGGGTTCGAATTGGATTAATTGTTGCAATGATCGTGCCATTGACGGCGTGTGGGACGAGTACGAG
TCGCAAAAATGCTGCTAGTAGCCGACCCAAACCAACTAAGGTCGTCAAGACGCCGATCGAGGATACCCAGTTCATGATGG
GCACAGTTGTCACGTTGCGGATTTATAATAAGGGTAAGGATAACGTGTTAGCTGGGGCGTTTAAGCTGTTGAAAACGGAA
GCTAAGGAAATTACGGTCAATCAAAAAGGATCTGAAATTGATAAGATCAACGCCGCTGCCGGTAAAAAGCCAGTGGCTGT
CAGCAAGGATATCTACCCAATGATCAAAGCAGCTTACTATTATAGTAAGAATTCAGACGAATCCTTTGATTTAGCCATTG
GCCCGATCACATCACTCTGGCATATTGGATTTTCTGATGCCAAGGTGCCCACTCAACAAGAAATTGACAAGAATGTTAAG
CTGGTAGACTACAAAAAAGTTAAATTAAATGACAAGAAACGGACCGTTTATCTAGAAGAAAAGGGAATGCAACTAGACCT
TGGTGGGATTGCCAAGGGTTACATGACCGATCAAGTGAAGACGTACTTATTAAATCATGGGGTTTCAACGGCCATTATTG
ACCTCGGTGGTAATATTTATGTTCTGGGTGACAGCCCTAAAGGAACGAAGTCAGGTAACTGGACAGTCGGTATCCAAGAT
CCAAAAGCGTCACGGGGTACGGCCATTGGCTCACTACCTGCTAAGAATAAGTCAATCGTTACTTCTGGGATCTATGAACG
CTATTTGAAGAAGAATGGTAAAGTTTACAGTCATTTGATGGACCCACAAACAGGGGCCCCTTATCAAAACAATTTGATGG
GCGTTTCCATCATCTCGAAGAAGTCGGTGGATGGCGATGCACTATCGACGGCCACCTTTGACAAAGGCCTCAAAGGCGGG
ATGAAGTACATTAATGGTAAAGCGGATCAAGGTATTGGGGCCATTTTTATTACCAAGGATAAAAAAGTATACGTCTCCAA
TAACTTGAAGAAGCAGTTCACGCTGTTTGGCGATGCTGGTTACAAATACGGGCCAGCCACTGATTTAAAGTAA

Upstream 100 bases:

>100_bases
CTAGCGTAGAAAAGCTTGGATGACGCGCAAACAGTCTCACCGGATGAATCAAGACTTTGGTATAATGACAATTGAAATTA
AAAATAGGGGAAGTATTGGA

Downstream 100 bases:

>100_bases
GATTGGACGTGAAGTTCAGTTACTACAAGTTCCGTCAGATATGTTATGATAATAACCATGAATTTTAAGGAAATGAGGGA
CAATGGTGAGTCCGATTATT

Product: thiamin biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKLKKWVRIGLIVAMIVPLTACGTSTSRKNAASSRPKPTKVVKTPIEDTQFMMGTVVTLRIYNKGKDNVLAGAFKLLKTE
AKEITVNQKGSEIDKINAAAGKKPVAVSKDIYPMIKAAYYYSKNSDESFDLAIGPITSLWHIGFSDAKVPTQQEIDKNVK
LVDYKKVKLNDKKRTVYLEEKGMQLDLGGIAKGYMTDQVKTYLLNHGVSTAIIDLGGNIYVLGDSPKGTKSGNWTVGIQD
PKASRGTAIGSLPAKNKSIVTSGIYERYLKKNGKVYSHLMDPQTGAPYQNNLMGVSIISKKSVDGDALSTATFDKGLKGG
MKYINGKADQGIGAIFITKDKKVYVSNNLKKQFTLFGDAGYKYGPATDLK

Sequences:

>Translated_370_residues
MKLKKWVRIGLIVAMIVPLTACGTSTSRKNAASSRPKPTKVVKTPIEDTQFMMGTVVTLRIYNKGKDNVLAGAFKLLKTE
AKEITVNQKGSEIDKINAAAGKKPVAVSKDIYPMIKAAYYYSKNSDESFDLAIGPITSLWHIGFSDAKVPTQQEIDKNVK
LVDYKKVKLNDKKRTVYLEEKGMQLDLGGIAKGYMTDQVKTYLLNHGVSTAIIDLGGNIYVLGDSPKGTKSGNWTVGIQD
PKASRGTAIGSLPAKNKSIVTSGIYERYLKKNGKVYSHLMDPQTGAPYQNNLMGVSIISKKSVDGDALSTATFDKGLKGG
MKYINGKADQGIGAIFITKDKKVYVSNNLKKQFTLFGDAGYKYGPATDLK
>Mature_370_residues
MKLKKWVRIGLIVAMIVPLTACGTSTSRKNAASSRPKPTKVVKTPIEDTQFMMGTVVTLRIYNKGKDNVLAGAFKLLKTE
AKEITVNQKGSEIDKINAAAGKKPVAVSKDIYPMIKAAYYYSKNSDESFDLAIGPITSLWHIGFSDAKVPTQQEIDKNVK
LVDYKKVKLNDKKRTVYLEEKGMQLDLGGIAKGYMTDQVKTYLLNHGVSTAIIDLGGNIYVLGDSPKGTKSGNWTVGIQD
PKASRGTAIGSLPAKNKSIVTSGIYERYLKKNGKVYSHLMDPQTGAPYQNNLMGVSIISKKSVDGDALSTATFDKGLKGG
MKYINGKADQGIGAIFITKDKKVYVSNNLKKQFTLFGDAGYKYGPATDLK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=284, Percent_Identity=23.5915492957746, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 40273; Mature: 40273

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKKWVRIGLIVAMIVPLTACGTSTSRKNAASSRPKPTKVVKTPIEDTQFMMGTVVTLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHEEEEEEEE
IYNKGKDNVLAGAFKLLKTEAKEITVNQKGSEIDKINAAAGKKPVAVSKDIYPMIKAAYY
EECCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCEECCCHHHHHHHHEE
YSKNSDESFDLAIGPITSLWHIGFSDAKVPTQQEIDKNVKLVDYKKVKLNDKKRTVYLEE
ECCCCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHCCCCEEEEEEEEEECCCEEEEEEEC
KGMQLDLGGIAKGYMTDQVKTYLLNHGVSTAIIDLGGNIYVLGDSPKGTKSGNWTVGIQD
CCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEEEEECC
PKASRGTAIGSLPAKNKSIVTSGIYERYLKKNGKVYSHLMDPQTGAPYQNNLMGVSIISK
CCCCCCCEECCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEC
KSVDGDALSTATFDKGLKGGMKYINGKADQGIGAIFITKDKKVYVSNNLKKQFTLFGDAG
CCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEECCCCEEEEEEECCC
YKYGPATDLK
CCCCCCCCCC
>Mature Secondary Structure
MKLKKWVRIGLIVAMIVPLTACGTSTSRKNAASSRPKPTKVVKTPIEDTQFMMGTVVTLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHEEEEEEEE
IYNKGKDNVLAGAFKLLKTEAKEITVNQKGSEIDKINAAAGKKPVAVSKDIYPMIKAAYY
EECCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCCCCCEECCCHHHHHHHHEE
YSKNSDESFDLAIGPITSLWHIGFSDAKVPTQQEIDKNVKLVDYKKVKLNDKKRTVYLEE
ECCCCCCCEEEEECCHHHHHCCCCCCCCCCCHHHHCCCCEEEEEEEEEECCCEEEEEEEC
KGMQLDLGGIAKGYMTDQVKTYLLNHGVSTAIIDLGGNIYVLGDSPKGTKSGNWTVGIQD
CCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEEEEECC
PKASRGTAIGSLPAKNKSIVTSGIYERYLKKNGKVYSHLMDPQTGAPYQNNLMGVSIISK
CCCCCCCEECCCCCCCCCEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEC
KSVDGDALSTATFDKGLKGGMKYINGKADQGIGAIFITKDKKVYVSNNLKKQFTLFGDAG
CCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEECCCEEEEECCCCEEEEEEECCC
YKYGPATDLK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]