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The map label for this gene is glnH1 [H]

Identifier: 28377714

GI number: 28377714

Start: 812631

End: 813467

Strand: Direct

Name: glnH1 [H]

Synonym: lp_0881

Alternate gene names: 28377714

Gene position: 812631-813467 (Clockwise)

Preceding gene: 28377713

Following gene: 28377715

Centisome position: 24.56

GC content: 47.19

Gene sequence:

>837_bases
ATGAAGAAGCTGAAACGACTAATTGGCGCACTCGGCATGCTTGCCATGCTAGTGGTGGTCTTAACCGCTTGCGGTTCACG
GCAATCATTAGCAAAACAGGATGTTTTGACCAATGATAAAGCTAGTAAGACGATTACGTGGGGTGTTAAAGCTGATACGA
AATTATTTGGGTTGATGGACGTTAAAGATAATCAAATCAAGGGGTTTGACGCCGACATTGCGACGGCATTAACAAAGCGG
GTGCTCGGTAAGAATGCTACCGCCAAGTTTGTCCAAGTAACGAGTCAAACGCGGATTCCATTGTTGAAGAATGGGAACAT
TGATGCCATTCTCGCCACGATGACTATCACGCCGGAACGTGAAAAACAGGTTGATTTTACGAATTCATACTTTGATGCGG
GGCAATCACTGTTGGTTAAAAAGGGCAGTGCCATAAAATCAGTTAAGGATTTGAATAAGTCGGGCACCAAGGTCCTTGGT
GTGACCGGTGCAAACTCCGTTGAGAACATTAAGAAAGCAGCGCCTAAGGCTAAGGTGTTGGAATTATCCGACTATGCTCA
GGCAATGACGGCGTTGAAATCTGGTCAGGGGGTCGCGTTGACTACTGATAACGGGATTCTTTACGGGATGGCGTCGCAAA
ACCCAGGGTACGAAGTGGTTGGGGGCACCTTCACGAAAGAACCATACGGGATCGCTGTTAATAAGGGTCAGGAACCGTTA
AAAAAGGCTCTGAATAAAGCGCTCAAAGAAATTGAAGCGGACGGGACGTACAACCGCATCTTGAAGAAGTGGTTTGGTAA
CGTGGCCGGCTTCGACTATAAGGAGGCGTCACGCTAA

Upstream 100 bases:

>100_bases
ATGCTAAAGAAAAGTTCTTTGATGGTCAGCCGTCTAATGAACGGGCCCGTCAATTCTTGAGTAAAATTATTACACACTAA
TTTGTAGGGGAGGACCACGA

Downstream 100 bases:

>100_bases
TGGGATACATTCTAACGCACTATTGGTCAGAGCTGATTCAAGGACTCGGATATACGTTGTTATCCAGTGTGATTGCTTTG
GTATTCAGTACGATCATCGG

Product: glutamine ABC transporter, substrate binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKKLKRLIGALGMLAMLVVVLTACGSRQSLAKQDVLTNDKASKTITWGVKADTKLFGLMDVKDNQIKGFDADIATALTKR
VLGKNATAKFVQVTSQTRIPLLKNGNIDAILATMTITPEREKQVDFTNSYFDAGQSLLVKKGSAIKSVKDLNKSGTKVLG
VTGANSVENIKKAAPKAKVLELSDYAQAMTALKSGQGVALTTDNGILYGMASQNPGYEVVGGTFTKEPYGIAVNKGQEPL
KKALNKALKEIEADGTYNRILKKWFGNVAGFDYKEASR

Sequences:

>Translated_278_residues
MKKLKRLIGALGMLAMLVVVLTACGSRQSLAKQDVLTNDKASKTITWGVKADTKLFGLMDVKDNQIKGFDADIATALTKR
VLGKNATAKFVQVTSQTRIPLLKNGNIDAILATMTITPEREKQVDFTNSYFDAGQSLLVKKGSAIKSVKDLNKSGTKVLG
VTGANSVENIKKAAPKAKVLELSDYAQAMTALKSGQGVALTTDNGILYGMASQNPGYEVVGGTFTKEPYGIAVNKGQEPL
KKALNKALKEIEADGTYNRILKKWFGNVAGFDYKEASR
>Mature_278_residues
MKKLKRLIGALGMLAMLVVVLTACGSRQSLAKQDVLTNDKASKTITWGVKADTKLFGLMDVKDNQIKGFDADIATALTKR
VLGKNATAKFVQVTSQTRIPLLKNGNIDAILATMTITPEREKQVDFTNSYFDAGQSLLVKKGSAIKSVKDLNKSGTKVLG
VTGANSVENIKKAAPKAKVLELSDYAQAMTALKSGQGVALTTDNGILYGMASQNPGYEVVGGTFTKEPYGIAVNKGQEPL
KKALNKALKEIEADGTYNRILKKWFGNVAGFDYKEASR

Specific function: Part of the ABC transporter complex glnHMPQ involved in glutamine transport [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=230, Percent_Identity=34.7826086956522, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=32.6086956521739, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787031, Length=203, Percent_Identity=33.9901477832512, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786876, Length=216, Percent_Identity=30.0925925925926, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788228, Length=262, Percent_Identity=29.7709923664122, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788648, Length=210, Percent_Identity=30, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788649, Length=269, Percent_Identity=27.8810408921933, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29869; Mature: 29869

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKRLIGALGMLAMLVVVLTACGSRQSLAKQDVLTNDKASKTITWGVKADTKLFGLMD
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEE
VKDNQIKGFDADIATALTKRVLGKNATAKFVQVTSQTRIPLLKNGNIDAILATMTITPER
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEECCCCCCCEEECCCCCEEEEEEEECCCH
EKQVDFTNSYFDAGQSLLVKKGSAIKSVKDLNKSGTKVLGVTGANSVENIKKAAPKAKVL
HCCCCCCHHHHHCCCCEEHCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCEEE
ELSDYAQAMTALKSGQGVALTTDNGILYGMASQNPGYEVVGGTFTKEPYGIAVNKGQEPL
EHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCEEECCEECCCCCCEEECCCHHHH
KKALNKALKEIEADGTYNRILKKWFGNVAGFDYKEASR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure
MKKLKRLIGALGMLAMLVVVLTACGSRQSLAKQDVLTNDKASKTITWGVKADTKLFGLMD
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEE
VKDNQIKGFDADIATALTKRVLGKNATAKFVQVTSQTRIPLLKNGNIDAILATMTITPER
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEECCCCCCCEEECCCCCEEEEEEEECCCH
EKQVDFTNSYFDAGQSLLVKKGSAIKSVKDLNKSGTKVLGVTGANSVENIKKAAPKAKVL
HCCCCCCHHHHHCCCCEEHCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCEEE
ELSDYAQAMTALKSGQGVALTTDNGILYGMASQNPGYEVVGGTFTKEPYGIAVNKGQEPL
EHHHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCCCEEECCEECCCCCCEEECCCHHHH
KKALNKALKEIEADGTYNRILKKWFGNVAGFDYKEASR
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 9384377 [H]