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The map label for this gene is patA [H]

Identifier: 28377711

GI number: 28377711

Start: 809221

End: 810852

Strand: Direct

Name: patA [H]

Synonym: lp_0874

Alternate gene names: 28377711

Gene position: 809221-810852 (Clockwise)

Preceding gene: 28377709

Following gene: 28377713

Centisome position: 24.46

GC content: 48.35

Gene sequence:

>1632_bases
ATGTCAATTAATTCAGAAACACTTGTTAACCATATGAATCATGAAATTGCGGCGATTCAGCCGTCTGATATTTTGGCGTT
CAACGCTGAAATCGCAAATATTCCGGGTATCGTGCGTTTGACCCTCGGTGAACCGGATTTTAATACACCTGAGCACGTGA
AACAAGCTGCAATCGAAAGCATTGAAGCGGATGAAAGCCACTATGCACCCTCGAACGGAACGTTGGCCTTGCGGACGGCT
GCCGCTGAATTCTTGGCCGCTAAGTATGATGTCCATTATGATCCTGCTAGCGAAGTGATTATCACAGCTGGTGCGACCGG
GGGCATTTATACAGCGTTGACTTCAATCCTTAATCCTGGGGATGAGGTCCTGATTCCAACCCCAATTTTTCCATTGTACA
TCGCAATCGTTAAATTATCTGGGGCAACACCAGTCTTCATGGATACCTCGGACAATGGCTTCGTGTTATCGCCTGACCAG
TTACAAACGACGTTAGCAGCCCACCCCAAGACGAAAGCGGTGGTCCTTAACTTTCCTTCAAATCCAACCGGTGTGACTTA
CCGGCATGATGATCTGAAAGCCCTGGCCGCAGTGTTAGCTGATCAACCAATTTTTGTTTTGTCTGATGAAATTTATAGCG
AATTGACGTATGGTGAGCCTCATGAATCGATTGCAAATTACTTACCAACACAAACTATTTTGCTTAACGGCGTCTCAAAG
TCGCACGCGATGACGGGCTGGCGAATCGGCATCATGTGTGCGCCGAAAGCTATCACAGCACAACTGGGTAAGATTCACCA
ATTCACAGTGACTTCAACGACGACCAATGCTCAAGCAGCTGCGGCCGGGTTAAAACAACTAACGAGTCGTACGGCTGGTG
TGGATACAATCGATATTCCGGCAGCCAAGGCGGCTGGTCTTGTGGTCACGAACGTCCCAGCCTATTCGCCTAATTCAGTT
GCTGAGATGTCGGTGGCACAAACCATGCGCTTGATTCGCAACTTGGAAATGTTTGATCAACGAATCAGCCAACAGAATTT
TCAGTGGGCGGGACTGCAAGCACGAGAAATTCGATCATTAACCGTTGGTATTATTGGTGCGGGTCGTATTGGTGGCACGG
CCGCTCGACTTTTCCACGGGTTAGGTGCCAAGGTGATTGCCTATGACGTGGTACGCCATCCTGAACTCGAAGACGTTTTA
ACCTACGTTGACACTAAGGAAGACCTATTGCGGCAGGCGGATGTCGTTGATTTACACGTGGATTTGAATGAGACCTCTGC
GGGGTTGATCGATGCGGCAGCCTTGAAGTTAATGAAGACGGATGCCTACCTTATCAACGCCTCACGCGGACCAGTGATTG
TCACCGATGATCTTGTGGCAGCGCTAAAGGCCGGTGAAATCGCCGGTTGTGCACTCGATACGGTTGAGGGTGAAAATGCG
TTATTTAACCAAAATCATCAGGGTGAGGTGCTTCAAGATACCAACGTGGCACAATTAATGCAGATGCCCAACGTCATTAT
CACGCCACACGTGGGTTTCTATACGAATTTAGCGGTCAAAAACATGGTCGATATTAGCCTGGATGACGTCTTAGCAATTC
TAAATGGTGAAACGACGGCCCACGCCTTTTAA

Upstream 100 bases:

>100_bases
CGTGGTGGCGCACCCTTTGGAATTTCAAGGGGTGCGCTTTTTTGACCCTAGTCCAGTCCGCTTCCACCAACAAGATCAGC
CAAACTTGGGAGGAATCTTT

Downstream 100 bases:

>100_bases
TGATGGCGTCAGCCGCTACTACCAGTATGGCTGTAGGAGACGGTGGCTGGTCAAAGTGCCCCACCGGTTAACTTGTTCGG
CGACTCGCTGTTCGATAACT

Product: bifunctional protein: amino acid aminotransferase; 2-hydroxyacid dehydrogenase

Products: H+; Nicotinamide adenine dinucleotide - reduced; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSINSETLVNHMNHEIAAIQPSDILAFNAEIANIPGIVRLTLGEPDFNTPEHVKQAAIESIEADESHYAPSNGTLALRTA
AAEFLAAKYDVHYDPASEVIITAGATGGIYTALTSILNPGDEVLIPTPIFPLYIAIVKLSGATPVFMDTSDNGFVLSPDQ
LQTTLAAHPKTKAVVLNFPSNPTGVTYRHDDLKALAAVLADQPIFVLSDEIYSELTYGEPHESIANYLPTQTILLNGVSK
SHAMTGWRIGIMCAPKAITAQLGKIHQFTVTSTTTNAQAAAAGLKQLTSRTAGVDTIDIPAAKAAGLVVTNVPAYSPNSV
AEMSVAQTMRLIRNLEMFDQRISQQNFQWAGLQAREIRSLTVGIIGAGRIGGTAARLFHGLGAKVIAYDVVRHPELEDVL
TYVDTKEDLLRQADVVDLHVDLNETSAGLIDAAALKLMKTDAYLINASRGPVIVTDDLVAALKAGEIAGCALDTVEGENA
LFNQNHQGEVLQDTNVAQLMQMPNVIITPHVGFYTNLAVKNMVDISLDDVLAILNGETTAHAF

Sequences:

>Translated_543_residues
MSINSETLVNHMNHEIAAIQPSDILAFNAEIANIPGIVRLTLGEPDFNTPEHVKQAAIESIEADESHYAPSNGTLALRTA
AAEFLAAKYDVHYDPASEVIITAGATGGIYTALTSILNPGDEVLIPTPIFPLYIAIVKLSGATPVFMDTSDNGFVLSPDQ
LQTTLAAHPKTKAVVLNFPSNPTGVTYRHDDLKALAAVLADQPIFVLSDEIYSELTYGEPHESIANYLPTQTILLNGVSK
SHAMTGWRIGIMCAPKAITAQLGKIHQFTVTSTTTNAQAAAAGLKQLTSRTAGVDTIDIPAAKAAGLVVTNVPAYSPNSV
AEMSVAQTMRLIRNLEMFDQRISQQNFQWAGLQAREIRSLTVGIIGAGRIGGTAARLFHGLGAKVIAYDVVRHPELEDVL
TYVDTKEDLLRQADVVDLHVDLNETSAGLIDAAALKLMKTDAYLINASRGPVIVTDDLVAALKAGEIAGCALDTVEGENA
LFNQNHQGEVLQDTNVAQLMQMPNVIITPHVGFYTNLAVKNMVDISLDDVLAILNGETTAHAF
>Mature_542_residues
SINSETLVNHMNHEIAAIQPSDILAFNAEIANIPGIVRLTLGEPDFNTPEHVKQAAIESIEADESHYAPSNGTLALRTAA
AEFLAAKYDVHYDPASEVIITAGATGGIYTALTSILNPGDEVLIPTPIFPLYIAIVKLSGATPVFMDTSDNGFVLSPDQL
QTTLAAHPKTKAVVLNFPSNPTGVTYRHDDLKALAAVLADQPIFVLSDEIYSELTYGEPHESIANYLPTQTILLNGVSKS
HAMTGWRIGIMCAPKAITAQLGKIHQFTVTSTTTNAQAAAAGLKQLTSRTAGVDTIDIPAAKAAGLVVTNVPAYSPNSVA
EMSVAQTMRLIRNLEMFDQRISQQNFQWAGLQAREIRSLTVGIIGAGRIGGTAARLFHGLGAKVIAYDVVRHPELEDVLT
YVDTKEDLLRQADVVDLHVDLNETSAGLIDAAALKLMKTDAYLINASRGPVIVTDDLVAALKAGEIAGCALDTVEGENAL
FNQNHQGEVLQDTNVAQLMQMPNVIITPHVGFYTNLAVKNMVDISLDDVLAILNGETTAHAF

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI56713254, Length=271, Percent_Identity=29.520295202952, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI56713256, Length=271, Percent_Identity=29.520295202952, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI95147551, Length=286, Percent_Identity=29.020979020979, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI169881279, Length=286, Percent_Identity=29.020979020979, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI145580578, Length=291, Percent_Identity=30.5841924398625, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI4557499, Length=291, Percent_Identity=30.5841924398625, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI61743967, Length=295, Percent_Identity=31.1864406779661, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI4557497, Length=295, Percent_Identity=31.1864406779661, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI145580575, Length=291, Percent_Identity=30.5841924398625, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI6912396, Length=266, Percent_Identity=29.6992481203008, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI23308577, Length=251, Percent_Identity=27.4900398406374, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4507369, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI169881281, Length=198, Percent_Identity=29.2929292929293, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI1787645, Length=260, Percent_Identity=33.4615384615385, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1786816, Length=250, Percent_Identity=31.2, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788722, Length=249, Percent_Identity=29.3172690763052, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789279, Length=252, Percent_Identity=31.3492063492063, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788627, Length=225, Percent_Identity=30.2222222222222, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082289, Length=263, Percent_Identity=27.7566539923954, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081824, Length=248, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=249, Percent_Identity=30.5220883534137, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994472, Length=260, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994476, Length=260, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25147481, Length=258, Percent_Identity=27.5193798449612, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17567369, Length=261, Percent_Identity=28.735632183908, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17567663, Length=225, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320925, Length=246, Percent_Identity=34.9593495934959, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=224, Percent_Identity=35.7142857142857, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322401, Length=232, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=246, Percent_Identity=28.0487804878049, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324964, Length=244, Percent_Identity=28.6885245901639, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323118, Length=169, Percent_Identity=30.1775147928994, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=154, Percent_Identity=28.5714285714286, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI62472511, Length=288, Percent_Identity=30.9027777777778, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646446, Length=290, Percent_Identity=30.6896551724138, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24646448, Length=290, Percent_Identity=30.6896551724138, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24646452, Length=290, Percent_Identity=30.6896551724138, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24646450, Length=290, Percent_Identity=30.6896551724138, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28573069, Length=262, Percent_Identity=27.8625954198473, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24646114, Length=262, Percent_Identity=27.8625954198473, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573067, Length=262, Percent_Identity=27.8625954198473, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573065, Length=262, Percent_Identity=27.8625954198473, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574286, Length=268, Percent_Identity=27.2388059701493, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI28574284, Length=265, Percent_Identity=28.3018867924528, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585514, Length=265, Percent_Identity=28.3018867924528, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574282, Length=265, Percent_Identity=28.3018867924528, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45551003, Length=265, Percent_Identity=28.3018867924528, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45552429, Length=266, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921140, Length=268, Percent_Identity=27.2388059701493, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI18859735, Length=289, Percent_Identity=25.2595155709343, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585516, Length=267, Percent_Identity=24.7191011235955, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 1.1.1.28 [C]

Molecular weight: Translated: 58002; Mature: 57870

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3 ; PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINSETLVNHMNHEIAAIQPSDILAFNAEIANIPGIVRLTLGEPDFNTPEHVKQAAIES
CCCCHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IEADESHYAPSNGTLALRTAAAEFLAAKYDVHYDPASEVIITAGATGGIYTALTSILNPG
HCCCCCCCCCCCCEEEEHHHHHHHHHHHCCEEECCCCCEEEEECCCCHHHHHHHHHHCCC
DEVLIPTPIFPLYIAIVKLSGATPVFMDTSDNGFVLSPDQLQTTLAAHPKTKAVVLNFPS
CCEEECCCHHHHHHHHHEECCCCEEEEECCCCCEEECHHHHHHHHHCCCCCCEEEEECCC
NPTGVTYRHDDLKALAAVLADQPIFVLSDEIYSELTYGEPHESIANYLPTQTILLNGVSK
CCCCEEEECHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCC
SHAMTGWRIGIMCAPKAITAQLGKIHQFTVTSTTTNAQAAAAGLKQLTSRTAGVDTIDIP
CCCCCCEEEEEEECCHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECC
AAKAAGLVVTNVPAYSPNSVAEMSVAQTMRLIRNLEMFDQRISQQNFQWAGLQAREIRSL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHCEE
TVGIIGAGRIGGTAARLFHGLGAKVIAYDVVRHPELEDVLTYVDTKEDLLRQADVVDLHV
EEEEEECCCCCHHHHHHHHHCCCCEEEEHHHCCCCHHHHHHHCCCHHHHHHCCCEEEEEE
DLNETSAGLIDAAALKLMKTDAYLINASRGPVIVTDDLVAALKAGEIAGCALDTVEGENA
ECCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHHHHHCCCCCCEEEECCCCCCC
LFNQNHQGEVLQDTNVAQLMQMPNVIITPHVGFYTNLAVKNMVDISLDDVLAILNGETTA
EECCCCCCCEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHEECCCHHHHHHHHCCCCCC
HAF
CCC
>Mature Secondary Structure 
SINSETLVNHMNHEIAAIQPSDILAFNAEIANIPGIVRLTLGEPDFNTPEHVKQAAIES
CCCHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
IEADESHYAPSNGTLALRTAAAEFLAAKYDVHYDPASEVIITAGATGGIYTALTSILNPG
HCCCCCCCCCCCCEEEEHHHHHHHHHHHCCEEECCCCCEEEEECCCCHHHHHHHHHHCCC
DEVLIPTPIFPLYIAIVKLSGATPVFMDTSDNGFVLSPDQLQTTLAAHPKTKAVVLNFPS
CCEEECCCHHHHHHHHHEECCCCEEEEECCCCCEEECHHHHHHHHHCCCCCCEEEEECCC
NPTGVTYRHDDLKALAAVLADQPIFVLSDEIYSELTYGEPHESIANYLPTQTILLNGVSK
CCCCEEEECHHHHHHHHHHCCCCEEEECHHHHHHCCCCCCHHHHHHHCCCCEEEEECCCC
SHAMTGWRIGIMCAPKAITAQLGKIHQFTVTSTTTNAQAAAAGLKQLTSRTAGVDTIDIP
CCCCCCEEEEEEECCHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECC
AAKAAGLVVTNVPAYSPNSVAEMSVAQTMRLIRNLEMFDQRISQQNFQWAGLQAREIRSL
CCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHCEE
TVGIIGAGRIGGTAARLFHGLGAKVIAYDVVRHPELEDVLTYVDTKEDLLRQADVVDLHV
EEEEEECCCCCHHHHHHHHHCCCCEEEEHHHCCCCHHHHHHHCCCHHHHHHCCCEEEEEE
DLNETSAGLIDAAALKLMKTDAYLINASRGPVIVTDDLVAALKAGEIAGCALDTVEGENA
ECCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEHHHHHHHHCCCCCCEEEECCCCCCC
LFNQNHQGEVLQDTNVAQLMQMPNVIITPHVGFYTNLAVKNMVDISLDDVLAILNGETTA
EECCCCCCCEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHEECCCHHHHHHHHCCCCCC
HAF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Lactate; Nicotinamide adenine dinucleotide [C]

Specific reaction: D-Lactate + Nicotinamide adenine dinucleotide <==> H+ + Nicotinamide adenine dinucleotide - reduced + Pyruvate [C]

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 2104615 [H]