Click here to switch to the map view.

The map label for this gene is pth

Identifier: 28377423

GI number: 28377423

Start: 486761

End: 487318

Strand: Direct

Name: pth

Synonym: lp_0538

Alternate gene names: 28377423

Gene position: 486761-487318 (Clockwise)

Preceding gene: 28377419

Following gene: 28377424

Centisome position: 14.71

GC content: 43.19

Gene sequence:

>558_bases
ATGAAAATGATCGTTGGGTTAGGCAACATTGGGCGGCAATATGCCCAGACACGTCATAATGTTGGTTTTATGATTGTGGA
CGAATTAGCTAGCAAACTAAACGTTACGTTTCAAACGAGTAAATTTGAAGCACAGGTGGCAACGGCTTTTCAAGACGGTG
AGAAGATTTTACTGGTGAAACCCGCCACTTATATGAATGATTCTGGACGAGCGGTGGGACCGTTGATGAGCTATTACAAT
GTTGATCCTGCTGATTTGCTCGTTATACATGATGATTTAGATTTACCACTAGGAAAAGTACGCCTCAAACAAAAGGGATC
GGCGGGTGGTCATAATGGCATCAAGAGCATTATTAGTCATGTTGGTGATCAGCACTTCAAGCGCGTTAAAGTTGGTATTG
ACCATCCTCAGAAGATGAGTGTTGTTGATTACGTGTTGGGGAAATTTACGCCAGCCGAAGTGGCGAAGTTTGACGATGCT
AAGATTACGGCGCTAGCGGCGGTCGAAGCTTGGTTAGCAAATGATGATTTTACGGCAGTTATGAATCAATATAACTAG

Upstream 100 bases:

>100_bases
CAATACGACTTAGGTGGTATCGTTACTCAGAGTTGCCGAATCGGGTCGATTACCGGTTCGGCCTGTTTTGGTATGCAAAG
TAATAGGAGAAGATAAAATT

Downstream 100 bases:

>100_bases
CCCGGGAGGAAACGACAGAATGGATATTGAAACGATTGTTGAGCAAACGCCCGACTTTCAAACAATTTTAGCAGGTATCA
GCCCGCGTAGTCGGCAACTA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKMIVGLGNIGRQYAQTRHNVGFMIVDELASKLNVTFQTSKFEAQVATAFQDGEKILLVKPATYMNDSGRAVGPLMSYYN
VDPADLLVIHDDLDLPLGKVRLKQKGSAGGHNGIKSIISHVGDQHFKRVKVGIDHPQKMSVVDYVLGKFTPAEVAKFDDA
KITALAAVEAWLANDDFTAVMNQYN

Sequences:

>Translated_185_residues
MKMIVGLGNIGRQYAQTRHNVGFMIVDELASKLNVTFQTSKFEAQVATAFQDGEKILLVKPATYMNDSGRAVGPLMSYYN
VDPADLLVIHDDLDLPLGKVRLKQKGSAGGHNGIKSIISHVGDQHFKRVKVGIDHPQKMSVVDYVLGKFTPAEVAKFDDA
KITALAAVEAWLANDDFTAVMNQYN
>Mature_185_residues
MKMIVGLGNIGRQYAQTRHNVGFMIVDELASKLNVTFQTSKFEAQVATAFQDGEKILLVKPATYMNDSGRAVGPLMSYYN
VDPADLLVIHDDLDLPLGKVRLKQKGSAGGHNGIKSIISHVGDQHFKRVKVGIDHPQKMSVVDYVLGKFTPAEVAKFDDA
KITALAAVEAWLANDDFTAVMNQYN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=155, Percent_Identity=36.7741935483871, Blast_Score=104, Evalue=5e-23,
Organism=Escherichia coli, GI1787455, Length=186, Percent_Identity=41.3978494623656, Blast_Score=121, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321983, Length=163, Percent_Identity=31.2883435582822, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_LACPL (Q88Z39)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784315.1
- ProteinModelPortal:   Q88Z39
- SMR:   Q88Z39
- GeneID:   1061883
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0538
- NMPDR:   fig|220668.1.peg.449
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   LPLA220668:LP_0538-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20294; Mature: 20294

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIVGLGNIGRQYAQTRHNVGFMIVDELASKLNVTFQTSKFEAQVATAFQDGEKILLVK
CEEEEECCHHHHHHHHHHHCCCEEEHHHHHHHHEEEEECCHHHHHHHHHHCCCCEEEEEC
PATYMNDSGRAVGPLMSYYNVDPADLLVIHDDLDLPLGKVRLKQKGSAGGHNGIKSIISH
CCCEECCCCCCHHHHHHHCCCCCHHEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHH
VGDQHFKRVKVGIDHPQKMSVVDYVLGKFTPAEVAKFDDAKITALAAVEAWLANDDFTAV
HCHHHHHEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
MNQYN
HCCCC
>Mature Secondary Structure
MKMIVGLGNIGRQYAQTRHNVGFMIVDELASKLNVTFQTSKFEAQVATAFQDGEKILLVK
CEEEEECCHHHHHHHHHHHCCCEEEHHHHHHHHEEEEECCHHHHHHHHHHCCCCEEEEEC
PATYMNDSGRAVGPLMSYYNVDPADLLVIHDDLDLPLGKVRLKQKGSAGGHNGIKSIISH
CCCEECCCCCCHHHHHHHCCCCCHHEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHH
VGDQHFKRVKVGIDHPQKMSVVDYVLGKFTPAEVAKFDDAKITALAAVEAWLANDDFTAV
HCHHHHHEEEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCHHHH
MNQYN
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566