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The map label for this gene is prs1

Identifier: 28377367

GI number: 28377367

Start: 420927

End: 421907

Strand: Direct

Name: prs1

Synonym: lp_0471

Alternate gene names: 28377367

Gene position: 420927-421907 (Clockwise)

Preceding gene: 28377366

Following gene: 28377368

Centisome position: 12.72

GC content: 43.53

Gene sequence:

>981_bases
ATGTCAGAACAGTATTTTGATCCAAAGTTAAAAATCTTTGCTTTGAATTCAAATAAACCATTGGCAGAAAAAATCGCGGA
CGCCGTTGGGGTTAAGTTGGGTAAGACTTCGGTCGATCGCTTTAGTGATGGCGAAATTCGCATTAACATTGAGGAAAGTA
TTCGTGGTGATCAAGTTTATATCATTCAATCAACTTCCGCTCCCGTTAATGACAATTTGATGGAACTATTGATCATGATT
GACGCCCTACGTCGCGCGAGTGCTAAGACCATTAACGTGGTTATTCCTTATTACGGATATGCGCGTCAAGATCGGAAAGC
ACGTTCTCGCGAACCAATTACCGCTAAGTTAGTTGCAAACATGCTGGAAACAGCCGGTGCAACACGGATTTTGGCACTCG
ATCTTCATGCTGCCCAAATTCAAGGCTTCTTCGATATTCCGTTGGATCACTTGATGGGTGCGCCACTGTTAGCTGACTAC
TTCCTGAACCACCACTTGGATGAAAATGCTGTGGTTGTTTCACCTGACCATGGTGGTGTGACTCGGGCACGTAAATTAGC
TGAATTCTTGAAGGCACCAATCGCGATTATTGATAAGCGTCGACCACGGGCCAACGTTGCCGAAGTTATGAATATTATCG
GTGATGTCAAGGGTAAGCGGGCCATCATGATCGATGATATGATCGATACGGCTGGGACGATTACTTTAGGTGCACAGGCA
CTGGTTGATGCGGGCGCAACTGAAGTTTACGCCAGTTGTACGCATCCAGTTCTGTCTGGTCCTGCCATCGAACGGATTGA
AAAGTCACCAATTAAGAAATTAGTTGTGACGGATTCAATTGAATTACCAGCTGCTAAGCGAATCGATAAGATCGAACAAG
TTTCAGTTGGTCAATTAATGGGGCAAGCAATCAAGTTTATCCATGAAAATAAACCAGTTAGTCCATTATTTAAGAATCGT
TTCCATAACGAAGAAAATTAA

Upstream 100 bases:

>100_bases
AGCCCAGTCTCACGCAATTTTGCCTTGTTGTTGGGACTTGATTTTCACTAGCAAAATGCCTATTATTTAGTAAGTAATAA
ATTTTCGAATGGAGGAAATT

Downstream 100 bases:

>100_bases
TTGACGAAGCGTCAAATTGGCAATTGCTGATTTGACGCTTTTTAAGTTGCAGTAATTTCTAAAAGTGATAGAGTAAAGGT
GTTGTTTGGTGGGCATTGTG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 1; Phosphoribosyl pyrophosphate synthase 1; P-Rib-PP synthase 1; PRPP synthase 1

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSEQYFDPKLKIFALNSNKPLAEKIADAVGVKLGKTSVDRFSDGEIRINIEESIRGDQVYIIQSTSAPVNDNLMELLIMI
DALRRASAKTINVVIPYYGYARQDRKARSREPITAKLVANMLETAGATRILALDLHAAQIQGFFDIPLDHLMGAPLLADY
FLNHHLDENAVVVSPDHGGVTRARKLAEFLKAPIAIIDKRRPRANVAEVMNIIGDVKGKRAIMIDDMIDTAGTITLGAQA
LVDAGATEVYASCTHPVLSGPAIERIEKSPIKKLVVTDSIELPAAKRIDKIEQVSVGQLMGQAIKFIHENKPVSPLFKNR
FHNEEN

Sequences:

>Translated_326_residues
MSEQYFDPKLKIFALNSNKPLAEKIADAVGVKLGKTSVDRFSDGEIRINIEESIRGDQVYIIQSTSAPVNDNLMELLIMI
DALRRASAKTINVVIPYYGYARQDRKARSREPITAKLVANMLETAGATRILALDLHAAQIQGFFDIPLDHLMGAPLLADY
FLNHHLDENAVVVSPDHGGVTRARKLAEFLKAPIAIIDKRRPRANVAEVMNIIGDVKGKRAIMIDDMIDTAGTITLGAQA
LVDAGATEVYASCTHPVLSGPAIERIEKSPIKKLVVTDSIELPAAKRIDKIEQVSVGQLMGQAIKFIHENKPVSPLFKNR
FHNEEN
>Mature_325_residues
SEQYFDPKLKIFALNSNKPLAEKIADAVGVKLGKTSVDRFSDGEIRINIEESIRGDQVYIIQSTSAPVNDNLMELLIMID
ALRRASAKTINVVIPYYGYARQDRKARSREPITAKLVANMLETAGATRILALDLHAAQIQGFFDIPLDHLMGAPLLADYF
LNHHLDENAVVVSPDHGGVTRARKLAEFLKAPIAIIDKRRPRANVAEVMNIIGDVKGKRAIMIDDMIDTAGTITLGAQAL
VDAGATEVYASCTHPVLSGPAIERIEKSPIKKLVVTDSIELPAAKRIDKIEQVSVGQLMGQAIKFIHENKPVSPLFKNRF
HNEEN

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=46.6666666666667, Blast_Score=287, Evalue=1e-77,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=45.2229299363057, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI28557709, Length=315, Percent_Identity=45.7142857142857, Blast_Score=282, Evalue=2e-76,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=44.794952681388, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=36.7052023121387, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=35.1744186046512, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI310128524, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310115209, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310118259, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI310119946, Length=140, Percent_Identity=34.2857142857143, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1787458, Length=315, Percent_Identity=50.1587301587302, Blast_Score=318, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=44.9044585987261, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17554702, Length=314, Percent_Identity=44.9044585987261, Blast_Score=280, Evalue=7e-76,
Organism=Caenorhabditis elegans, GI71989924, Length=314, Percent_Identity=44.9044585987261, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=44.8387096774194, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17570245, Length=333, Percent_Identity=33.6336336336336, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=44.2307692307692, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319403, Length=317, Percent_Identity=42.9022082018927, Blast_Score=248, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=41.3461538461538, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6322667, Length=198, Percent_Identity=41.9191919191919, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324511, Length=115, Percent_Identity=38.2608695652174, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=47.3015873015873, Blast_Score=285, Evalue=2e-77,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=44.3786982248521, Blast_Score=274, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24651458, Length=349, Percent_Identity=34.3839541547278, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24651456, Length=349, Percent_Identity=34.3839541547278, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI281362873, Length=349, Percent_Identity=34.3839541547278, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24651454, Length=349, Percent_Identity=34.3839541547278, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24651462, Length=370, Percent_Identity=31.6216216216216, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24651464, Length=370, Percent_Identity=31.6216216216216, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI45552010, Length=370, Percent_Identity=31.6216216216216, Blast_Score=160, Evalue=9e-40,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS1_LACPL (Q88Z84)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784259.1
- ProteinModelPortal:   Q88Z84
- SMR:   Q88Z84
- GeneID:   1061409
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0471
- NMPDR:   fig|220668.1.peg.393
- HOGENOM:   HBG519284
- OMA:   CATHAVF
- ProtClustDB:   PRK01259
- BioCyc:   LPLA220668:LP_0471-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_B
- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 35880; Mature: 35749

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQYFDPKLKIFALNSNKPLAEKIADAVGVKLGKTSVDRFSDGEIRINIEESIRGDQVY
CCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCCCCCEEE
IIQSTSAPVNDNLMELLIMIDALRRASAKTINVVIPYYGYARQDRKARSREPITAKLVAN
EEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHH
MLETAGATRILALDLHAAQIQGFFDIPLDHLMGAPLLADYFLNHHLDENAVVVSPDHGGV
HHHHCCCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCH
TRARKLAEFLKAPIAIIDKRRPRANVAEVMNIIGDVKGKRAIMIDDMIDTAGTITLGAQA
HHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCCCCEEECHHH
LVDAGATEVYASCTHPVLSGPAIERIEKSPIKKLVVTDSIELPAAKRIDKIEQVSVGQLM
HHHCCCHHHHHHCCCCCCCCHHHHHHHHCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHH
GQAIKFIHENKPVSPLFKNRFHNEEN
HHHHHHHHCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure 
SEQYFDPKLKIFALNSNKPLAEKIADAVGVKLGKTSVDRFSDGEIRINIEESIRGDQVY
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHCCCCCEEE
IIQSTSAPVNDNLMELLIMIDALRRASAKTINVVIPYYGYARQDRKARSREPITAKLVAN
EEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCHHHHHHHH
MLETAGATRILALDLHAAQIQGFFDIPLDHLMGAPLLADYFLNHHLDENAVVVSPDHGGV
HHHHCCCCEEEEEEEEHHHHCCEECCCHHHHCCCHHHHHHHHHCCCCCCEEEECCCCCCH
TRARKLAEFLKAPIAIIDKRRPRANVAEVMNIIGDVKGKRAIMIDDMIDTAGTITLGAQA
HHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCCCCEEECHHH
LVDAGATEVYASCTHPVLSGPAIERIEKSPIKKLVVTDSIELPAAKRIDKIEQVSVGQLM
HHHCCCHHHHHHCCCCCCCCHHHHHHHHCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHH
GQAIKFIHENKPVSPLFKNRFHNEEN
HHHHHHHHCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566