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The map label for this gene is ksgA

Identifier: 28377358

GI number: 28377358

Start: 411603

End: 412493

Strand: Direct

Name: ksgA

Synonym: lp_0458

Alternate gene names: 28377358

Gene position: 411603-412493 (Clockwise)

Preceding gene: 28377357

Following gene: 28377359

Centisome position: 12.44

GC content: 46.69

Gene sequence:

>891_bases
ATGATGAGTAGAGATTTAGATATTGCGAATCCCGCCCGCACACGGGCAATTATGCATACGTACGGGTTACAAGTTAAGAA
GAGTTTAGGACAGAATTTTTTAACTGATCAGAACGTGTTACACAACATTGTTGCCACTGCTGACATTGGAACCAATGATA
ACGTGATTGAAATTGGCCCTGGGATCGGGGCACTGACTGAGTATTTGGCACGGGCTGCGCACCACGTCCTGGCCTTTGAA
ATCGATGATCGGTTACTGCCAATTTTGGATGAGACACTAGCTGATTATGACAATGTAACGGTCGTTAACCAAGATATTTT
GAAGGCCGACTTAGCCGCGATGATCAGTGAACATTTAGATAACGAGCGGCCGTTGAAGCTCGTCGCCAATTTACCGTATT
ATATTACAACGCCTATTCTGATGAACATTTTGGCGGGGGACGTTGCTTTTGAAAATATTGTTGTGATGATGCAAAAGGAA
GTTGCCGACCGGTTAGCAGCTGAACCGGGTACGAAGGCGTATGGTGCGTTAACGATTGCTGTGCAGTACCGGATGGCTGC
TGAGATGGCGATGGTCGTTCCCCGGACGGTTTTCGTGCCGTCACCGAATGTCGATTCTGCAATCGTGAAATTGACGGCAT
TGCCACTGCGGACACACGTGCCATTTGACGAAGCAGCTTTTTTCAAGGTCGTTAAAGCGGGCTTTGCGCACCGTCGGAAA
AATCTGTGGAATAATTTGCAAAGCCTATTTGGTAAACAACCTGAGACGAAGACGGCCATTCAACAGGCTCTTGATATCGC
GACAATTGATCCCAAGATTCGTGCCGAACGGTTGACGGTCGATGAATTTATTACATTGACGGATGCATTACATCAGGCTG
ACTTACTTTAA

Upstream 100 bases:

>100_bases
GATACGTCAACGGTAAACAGCTGCCCAAACGGTTACAACTATTTCAAATTCAGCCAGCTGATTTTTGGGCGGCTGTTGAC
CAACTAACCACAGAGGAGAA

Downstream 100 bases:

>100_bases
GTTATCTATGCTTATAAAATGGTTGCACATTAGAAAACCTTGTGATATAATATTGACTTTTTTGTGAAAAGCTAGTATAG
TGTCTTTCATGAGGAGGATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MMSRDLDIANPARTRAIMHTYGLQVKKSLGQNFLTDQNVLHNIVATADIGTNDNVIEIGPGIGALTEYLARAAHHVLAFE
IDDRLLPILDETLADYDNVTVVNQDILKADLAAMISEHLDNERPLKLVANLPYYITTPILMNILAGDVAFENIVVMMQKE
VADRLAAEPGTKAYGALTIAVQYRMAAEMAMVVPRTVFVPSPNVDSAIVKLTALPLRTHVPFDEAAFFKVVKAGFAHRRK
NLWNNLQSLFGKQPETKTAIQQALDIATIDPKIRAERLTVDEFITLTDALHQADLL

Sequences:

>Translated_296_residues
MMSRDLDIANPARTRAIMHTYGLQVKKSLGQNFLTDQNVLHNIVATADIGTNDNVIEIGPGIGALTEYLARAAHHVLAFE
IDDRLLPILDETLADYDNVTVVNQDILKADLAAMISEHLDNERPLKLVANLPYYITTPILMNILAGDVAFENIVVMMQKE
VADRLAAEPGTKAYGALTIAVQYRMAAEMAMVVPRTVFVPSPNVDSAIVKLTALPLRTHVPFDEAAFFKVVKAGFAHRRK
NLWNNLQSLFGKQPETKTAIQQALDIATIDPKIRAERLTVDEFITLTDALHQADLL
>Mature_296_residues
MMSRDLDIANPARTRAIMHTYGLQVKKSLGQNFLTDQNVLHNIVATADIGTNDNVIEIGPGIGALTEYLARAAHHVLAFE
IDDRLLPILDETLADYDNVTVVNQDILKADLAAMISEHLDNERPLKLVANLPYYITTPILMNILAGDVAFENIVVMMQKE
VADRLAAEPGTKAYGALTIAVQYRMAAEMAMVVPRTVFVPSPNVDSAIVKLTALPLRTHVPFDEAAFFKVVKAGFAHRRK
NLWNNLQSLFGKQPETKTAIQQALDIATIDPKIRAERLTVDEFITLTDALHQADLL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=299, Percent_Identity=32.4414715719064, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI7657198, Length=247, Percent_Identity=29.5546558704453, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786236, Length=269, Percent_Identity=31.5985130111524, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25146882, Length=288, Percent_Identity=30.2083333333333, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=302, Percent_Identity=27.1523178807947, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=198, Percent_Identity=30.3030303030303, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21357273, Length=291, Percent_Identity=31.2714776632302, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21358017, Length=196, Percent_Identity=33.1632653061224, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_LACPL (Q88Z93)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784250.1
- ProteinModelPortal:   Q88Z93
- SMR:   Q88Z93
- GeneID:   1064147
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0458
- NMPDR:   fig|220668.1.peg.384
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   LPLA220668:LP_0458-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32705; Mature: 32705

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 32-32 BINDING 34-34 BINDING 59-59 BINDING 80-80 BINDING 105-105 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSRDLDIANPARTRAIMHTYGLQVKKSLGQNFLTDQNVLHNIVATADIGTNDNVIEIGP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHEECCCCCCEEEECC
GIGALTEYLARAAHHVLAFEIDDRLLPILDETLADYDNVTVVNQDILKADLAAMISEHLD
CHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCC
NERPLKLVANLPYYITTPILMNILAGDVAFENIVVMMQKEVADRLAAEPGTKAYGALTIA
CCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEE
VQYRMAAEMAMVVPRTVFVPSPNVDSAIVKLTALPLRTHVPFDEAAFFKVVKAGFAHRRK
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHH
NLWNNLQSLFGKQPETKTAIQQALDIATIDPKIRAERLTVDEFITLTDALHQADLL
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMSRDLDIANPARTRAIMHTYGLQVKKSLGQNFLTDQNVLHNIVATADIGTNDNVIEIGP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHEECCCCCCEEEECC
GIGALTEYLARAAHHVLAFEIDDRLLPILDETLADYDNVTVVNQDILKADLAAMISEHLD
CHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCC
NERPLKLVANLPYYITTPILMNILAGDVAFENIVVMMQKEVADRLAAEPGTKAYGALTIA
CCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEE
VQYRMAAEMAMVVPRTVFVPSPNVDSAIVKLTALPLRTHVPFDEAAFFKVVKAGFAHRRK
HHHHHHHHHHHHCCCEEECCCCCCCHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHH
NLWNNLQSLFGKQPETKTAIQQALDIATIDPKIRAERLTVDEFITLTDALHQADLL
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566