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The map label for this gene is cobB

Identifier: 28377351

GI number: 28377351

Start: 403836

End: 404540

Strand: Direct

Name: cobB

Synonym: lp_0449

Alternate gene names: 28377351

Gene position: 403836-404540 (Clockwise)

Preceding gene: 28377350

Following gene: 28377352

Centisome position: 12.21

GC content: 47.09

Gene sequence:

>705_bases
ATGAGTGACATTACAGCAGCACAAACAACTTTACAACAAGCTCAGCATATTGTTTTTATGACGGGGGCCGGCGTTTCGAC
GCCCTCGGGAATTCCTGATTACCGGTCTAAAAATGGACTGTATACCGAACACCATAACGCGGAGTATTATTTAAGCCACG
CGTTTTTGGCGGAGCATCCGCTGGAATTTTATCAATATTTACAATCAAATTTATATTATCCAGACGCCCAACCGAATGTG
ATTCATCAAAAGATGGCGGCACTGACTCAGCAAGGGCGGGCCAGTGTGATTACACAAAATATTGATAATTTATATGGGGT
GGCTAAAACGGCTCAGCTCGTTGAATTTCACGGTAATCTCTATCAGGTCTACTGCACCAAGTGTGGTCAGCATGTCGATT
GGCATGAGTATTTGAAGTCTCCTTACCATCAGACAGACCATGGCTACTTACGGCCTAACGTGGTGTTGTACGACGAGGGG
ATTGCGAGTGCCAATATCGAACGGGCAGTTCAATATTTACAGCAGGCTGACCTAGTCGTTATTTGTGGGACCTCGTTTCG
GGTGTACCCGTTCGCCGGCCTAATTGACTATCGTAATCCGAAGGCTCAAGTGCTTGCAATCAATGCGGAACCGCTCCAGT
TGCCATTTGCATTTACGATGGTGCAACAAGATGCGGTCGACTTCTTCGAGGGGGTGCAGGTCTGA

Upstream 100 bases:

>100_bases
TGCCTTTTTCAAAGAGGTGTACGATAAGACGGTCAAAGAATTATTCCAAGAAATGAATCTACCCTATACGTTACGGTAAG
CTAAGCAGGGAGATGTCAGA

Downstream 100 bases:

>100_bases
TGATAACAATTGGCTTAACAACTTGGACGGAACACCCTAGTTTACTGGGTGGTACTGAAAAGCTGACCTTAACTGAATAT
TCGGGAGTCCTACCAGTCGT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSDITAAQTTLQQAQHIVFMTGAGVSTPSGIPDYRSKNGLYTEHHNAEYYLSHAFLAEHPLEFYQYLQSNLYYPDAQPNV
IHQKMAALTQQGRASVITQNIDNLYGVAKTAQLVEFHGNLYQVYCTKCGQHVDWHEYLKSPYHQTDHGYLRPNVVLYDEG
IASANIERAVQYLQQADLVVICGTSFRVYPFAGLIDYRNPKAQVLAINAEPLQLPFAFTMVQQDAVDFFEGVQV

Sequences:

>Translated_234_residues
MSDITAAQTTLQQAQHIVFMTGAGVSTPSGIPDYRSKNGLYTEHHNAEYYLSHAFLAEHPLEFYQYLQSNLYYPDAQPNV
IHQKMAALTQQGRASVITQNIDNLYGVAKTAQLVEFHGNLYQVYCTKCGQHVDWHEYLKSPYHQTDHGYLRPNVVLYDEG
IASANIERAVQYLQQADLVVICGTSFRVYPFAGLIDYRNPKAQVLAINAEPLQLPFAFTMVQQDAVDFFEGVQV
>Mature_233_residues
SDITAAQTTLQQAQHIVFMTGAGVSTPSGIPDYRSKNGLYTEHHNAEYYLSHAFLAEHPLEFYQYLQSNLYYPDAQPNVI
HQKMAALTQQGRASVITQNIDNLYGVAKTAQLVEFHGNLYQVYCTKCGQHVDWHEYLKSPYHQTDHGYLRPNVVLYDEGI
ASANIERAVQYLQQADLVVICGTSFRVYPFAGLIDYRNPKAQVLAINAEPLQLPFAFTMVQQDAVDFFEGVQV

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=198, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI6912664, Length=213, Percent_Identity=32.8638497652582, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI13787215, Length=213, Percent_Identity=32.8638497652582, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI6912660, Length=240, Percent_Identity=31.25, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI63054862, Length=232, Percent_Identity=31.4655172413793, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI13775602, Length=219, Percent_Identity=29.2237442922374, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI13775600, Length=219, Percent_Identity=29.2237442922374, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI6912662, Length=225, Percent_Identity=25.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI300797705, Length=202, Percent_Identity=27.7227722772277, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI7706712, Length=231, Percent_Identity=27.7056277056277, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI308199517, Length=212, Percent_Identity=31.6037735849057, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17541892, Length=216, Percent_Identity=28.2407407407407, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17567771, Length=213, Percent_Identity=27.6995305164319, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71990482, Length=128, Percent_Identity=35.9375, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648389, Length=213, Percent_Identity=30.5164319248826, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571445, Length=141, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17137536, Length=246, Percent_Identity=25.2032520325203, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_LACPL (Q88ZA0)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784243.1
- ProteinModelPortal:   Q88ZA0
- SMR:   Q88ZA0
- GeneID:   1064153
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0449
- NMPDR:   fig|220668.1.peg.377
- HOGENOM:   HBG641281
- OMA:   LSHSFFM
- ProtClustDB:   PRK00481
- BioCyc:   LPLA220668:LP_0449-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26443; Mature: 26312

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 117-117

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDITAAQTTLQQAQHIVFMTGAGVSTPSGIPDYRSKNGLYTEHHNAEYYLSHAFLAEHP
CCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCEEEEHHHHHHHCH
LEFYQYLQSNLYYPDAQPNVIHQKMAALTQQGRASVITQNIDNLYGVAKTAQLVEFHGNL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
YQVYCTKCGQHVDWHEYLKSPYHQTDHGYLRPNVVLYDEGIASANIERAVQYLQQADLVV
EEEEEHHCCCCCCHHHHHCCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHCCEEE
ICGTSFRVYPFAGLIDYRNPKAQVLAINAEPLQLPFAFTMVQQDAVDFFEGVQV
EECCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDITAAQTTLQQAQHIVFMTGAGVSTPSGIPDYRSKNGLYTEHHNAEYYLSHAFLAEHP
CCCHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCEEEEHHHHHHHCH
LEFYQYLQSNLYYPDAQPNVIHQKMAALTQQGRASVITQNIDNLYGVAKTAQLVEFHGNL
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
YQVYCTKCGQHVDWHEYLKSPYHQTDHGYLRPNVVLYDEGIASANIERAVQYLQQADLVV
EEEEEHHCCCCCCHHHHHCCCCCCCCCCEECCCEEEEECCCCCCCHHHHHHHHHHCCEEE
ICGTSFRVYPFAGLIDYRNPKAQVLAINAEPLQLPFAFTMVQQDAVDFFEGVQV
EECCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566