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The map label for this gene is gplF2 [H]

Identifier: 28377111

GI number: 28377111

Start: 146413

End: 147120

Strand: Direct

Name: gplF2 [H]

Synonym: lp_0171

Alternate gene names: 28377111

Gene position: 146413-147120 (Clockwise)

Preceding gene: 28377110

Following gene: 28377112

Centisome position: 4.43

GC content: 46.05

Gene sequence:

>708_bases
ATGCACGGTTTTTTAGGTGAATTTTTAGGTACCATGGTGTTAATTGTATTTGGGGTTGGTAGTGGTGCCGCCATGAATTT
AAAGGGTAATTATGCACGCCATCAGAATTGGACGTTTATTTGCTTGGCGTGGGGCCTAGCCGTTACGTTTGGGGTCTATG
TTGCCGGACAATTTGGTTCGGATGGTCATCTAAATCCAGCGGTGACAGTTGGTTTTGCGTTATTCGGTTATTTGCCAATG
GCTAACGTTTGGCCGTACTTATTAGGCCAGTTCTTAGGGGCCTTTATCGGCGCGGTAATCGTTATTATTCAGTACTACCC
ACACTTTCAAGCTGCCAAAACGGCGGCAGATGGCAATCAAGTTGGCATTTTTGCAACTGGTCCAGCCATCAGTAACCCGG
TATTTAATTTTCTAAGCGAAACGATTGCGACCTTCTTCTTTATCTTCGTGTTGTTGAATCTCGGTAACTTTACCCAGGGA
TTAAAGCCATTGATGGTGGGGCTGCTCATTGTTGTCGTTGGTCAGACCTTGGGTGGAACCACAGGCTTTGCGATTAATCC
GGCGCGTGATTGGGCACCACGATTAGCCTACACGATTCTCCCGGTTCCTAATAAAGGGTTGGCTAATTGGGGTTATGCCT
GGGTTCCAATGTTTGGTCCATTGCTTGGTGGTATTTTGGCAGCTGGTCTGGAAACAATTATTAGTTAA

Upstream 100 bases:

>100_bases
GTGCCTACACGGCCGCATCGCTATTACAGGCGGCTGTGCCACTTGCGGATGTCGAAGCTCAATTAGCGCCATTAAAAATC
AAGTAGGATGGTGACGAAAC

Downstream 100 bases:

>100_bases
ATACTCACAACTGTGCCGATTTTGTTGGGCGCAGTTTTTTGTTATAAAGTATAATTAAATAAATAATGCAAGTTTATGCA
ATCGCTTGCAATAACGATGA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MHGFLGEFLGTMVLIVFGVGSGAAMNLKGNYARHQNWTFICLAWGLAVTFGVYVAGQFGSDGHLNPAVTVGFALFGYLPM
ANVWPYLLGQFLGAFIGAVIVIIQYYPHFQAAKTAADGNQVGIFATGPAISNPVFNFLSETIATFFFIFVLLNLGNFTQG
LKPLMVGLLIVVVGQTLGGTTGFAINPARDWAPRLAYTILPVPNKGLANWGYAWVPMFGPLLGGILAAGLETIIS

Sequences:

>Translated_235_residues
MHGFLGEFLGTMVLIVFGVGSGAAMNLKGNYARHQNWTFICLAWGLAVTFGVYVAGQFGSDGHLNPAVTVGFALFGYLPM
ANVWPYLLGQFLGAFIGAVIVIIQYYPHFQAAKTAADGNQVGIFATGPAISNPVFNFLSETIATFFFIFVLLNLGNFTQG
LKPLMVGLLIVVVGQTLGGTTGFAINPARDWAPRLAYTILPVPNKGLANWGYAWVPMFGPLLGGILAAGLETIIS
>Mature_235_residues
MHGFLGEFLGTMVLIVFGVGSGAAMNLKGNYARHQNWTFICLAWGLAVTFGVYVAGQFGSDGHLNPAVTVGFALFGYLPM
ANVWPYLLGQFLGAFIGAVIVIIQYYPHFQAAKTAADGNQVGIFATGPAISNPVFNFLSETIATFFFIFVLLNLGNFTQG
LKPLMVGLLIVVVGQTLGGTTGFAINPARDWAPRLAYTILPVPNKGLANWGYAWVPMFGPLLGGILAAGLETIIS

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=241, Percent_Identity=38.1742738589212, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI157266307, Length=243, Percent_Identity=37.8600823045267, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI4502187, Length=243, Percent_Identity=35.3909465020576, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI22538420, Length=231, Percent_Identity=33.7662337662338, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310133356, Length=203, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI310114181, Length=203, Percent_Identity=31.0344827586207, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=35.3174603174603, Blast_Score=111, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71994009, Length=234, Percent_Identity=32.0512820512821, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531429, Length=242, Percent_Identity=31.8181818181818, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=242, Percent_Identity=31.8181818181818, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71992966, Length=236, Percent_Identity=30.9322033898305, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=241, Percent_Identity=30.2904564315353, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32564052, Length=241, Percent_Identity=30.2904564315353, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71992961, Length=236, Percent_Identity=29.6610169491525, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17544068, Length=250, Percent_Identity=29.2, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=209, Percent_Identity=34.4497607655502, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322985, Length=278, Percent_Identity=27.6978417266187, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=29.4871794871795, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=29.4871794871795, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24999; Mature: 24999

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGFLGEFLGTMVLIVFGVGSGAAMNLKGNYARHQNWTFICLAWGLAVTFGVYVAGQFGS
CCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHEECCCC
DGHLNPAVTVGFALFGYLPMANVWPYLLGQFLGAFIGAVIVIIQYYPHFQAAKTAADGNQ
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCE
VGIFATGPAISNPVFNFLSETIATFFFIFVLLNLGNFTQGLKPLMVGLLIVVVGQTLGGT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
TGFAINPARDWAPRLAYTILPVPNKGLANWGYAWVPMFGPLLGGILAAGLETIIS
CCCEECCHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHGFLGEFLGTMVLIVFGVGSGAAMNLKGNYARHQNWTFICLAWGLAVTFGVYVAGQFGS
CCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHEECCCC
DGHLNPAVTVGFALFGYLPMANVWPYLLGQFLGAFIGAVIVIIQYYPHFQAAKTAADGNQ
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCE
VGIFATGPAISNPVFNFLSETIATFFFIFVLLNLGNFTQGLKPLMVGLLIVVVGQTLGGT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
TGFAINPARDWAPRLAYTILPVPNKGLANWGYAWVPMFGPLLGGILAAGLETIIS
CCCEECCHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]