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The map label for this gene is ydgI [H]

Identifier: 28377025

GI number: 28377025

Start: 61378

End: 62031

Strand: Direct

Name: ydgI [H]

Synonym: lp_0062

Alternate gene names: 28377025

Gene position: 61378-62031 (Clockwise)

Preceding gene: 28377024

Following gene: 28377026

Centisome position: 1.86

GC content: 46.02

Gene sequence:

>654_bases
ATGTCAGAAGCAGTAAAAAATTTGGTGAACAATGATTTAGCAGACGTAATGTTTAACCGCCATTCAGTTCGGCAGTTTGA
CCCGAACGTTAAAATTGGACGTGATGAGTTACAAAAGATGATTGCGGAAGCAGCCACCGCGCCATCGGCATGTAATTTAC
AGTCATGGCACTTTGTCGTCGTGGATACCCCCGAGGCAAAGGCTAAGTTCAAACAAGCCGTGATGAAATTCAACTACCCA
CAGGTCGACAGTGCATCGGCCATCGTCTTTATTGCCGGTGATACCCAGTCGCATTATGTTTATCGCGATGTCTGGAACAA
AGTTTATGAGGATGGGAATATTACGAAGGAACGCTTGGATCAGATTCTGGGAACCTTCTTACCATTATATGAAAATGCCA
CACCAGATTTCTTGAAATTCGATGCGACGATTGATTGTTCCGTTGTCGGGATGCAGTTGCTGCTAGTGGCACGGGCTCAT
GGGTATGATGCCAATGCGTTCTCCGGAATTGACTTTGAAAAGATGATTCCGACGCTGGGTCTTGATCCTAAACGATACGT
GCCAGTAATGGGAATCGCAATCGGGAAAGCAGCGCAAGAACCGCTTCATACGACTCGGTACGATGCCAAAACACAGACTG
ATTTCTTAGCCTAA

Upstream 100 bases:

>100_bases
AATGCCAAAGCTCTTAACGGGTCGGTATGACCGGAGTTTCTTCAATCCCAAGGCAGCGACTTACATTATTTAGGCGGATT
ACGAAAGCGAGGAATAGCTG

Downstream 100 bases:

>100_bases
TAGGTTTATGAAAAGAGCGGGATTCCCGCTCTTTTTTGTCCGAATACGGGCCTAAGAAAGCTGAGGACCTTTCGAGGGTG
GTGGGGGGTTATCAGTAGGC

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSEAVKNLVNNDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVVVDTPEAKAKFKQAVMKFNYP
QVDSASAIVFIAGDTQSHYVYRDVWNKVYEDGNITKERLDQILGTFLPLYENATPDFLKFDATIDCSVVGMQLLLVARAH
GYDANAFSGIDFEKMIPTLGLDPKRYVPVMGIAIGKAAQEPLHTTRYDAKTQTDFLA

Sequences:

>Translated_217_residues
MSEAVKNLVNNDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVVVDTPEAKAKFKQAVMKFNYP
QVDSASAIVFIAGDTQSHYVYRDVWNKVYEDGNITKERLDQILGTFLPLYENATPDFLKFDATIDCSVVGMQLLLVARAH
GYDANAFSGIDFEKMIPTLGLDPKRYVPVMGIAIGKAAQEPLHTTRYDAKTQTDFLA
>Mature_216_residues
SEAVKNLVNNDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVVVDTPEAKAKFKQAVMKFNYPQ
VDSASAIVFIAGDTQSHYVYRDVWNKVYEDGNITKERLDQILGTFLPLYENATPDFLKFDATIDCSVVGMQLLLVARAHG
YDANAFSGIDFEKMIPTLGLDPKRYVPVMGIAIGKAAQEPLHTTRYDAKTQTDFLA

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24270; Mature: 24139

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAVKNLVNNDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVV
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
VDTPEAKAKFKQAVMKFNYPQVDSASAIVFIAGDTQSHYVYRDVWNKVYEDGNITKERLD
EECCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHH
QILGTFLPLYENATPDFLKFDATIDCSVVGMQLLLVARAHGYDANAFSGIDFEKMIPTLG
HHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
LDPKRYVPVMGIAIGKAAQEPLHTTRYDAKTQTDFLA
CCCHHCCCHHHHHHHHHHHCCCHHHCCCCCCCHHCCC
>Mature Secondary Structure 
SEAVKNLVNNDLADVMFNRHSVRQFDPNVKIGRDELQKMIAEAATAPSACNLQSWHFVV
CHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
VDTPEAKAKFKQAVMKFNYPQVDSASAIVFIAGDTQSHYVYRDVWNKVYEDGNITKERLD
EECCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHH
QILGTFLPLYENATPDFLKFDATIDCSVVGMQLLLVARAHGYDANAFSGIDFEKMIPTLG
HHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
LDPKRYVPVMGIAIGKAAQEPLHTTRYDAKTQTDFLA
CCCHHCCCHHHHHHHHHHHCCCHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]