Click here to switch to the map view.

The map label for this gene is yflS [H]

Identifier: 28377019

GI number: 28377019

Start: 55625

End: 57040

Strand: Direct

Name: yflS [H]

Synonym: lp_0056

Alternate gene names: 28377019

Gene position: 55625-57040 (Clockwise)

Preceding gene: 28377015

Following gene: 28377020

Centisome position: 1.68

GC content: 42.87

Gene sequence:

>1416_bases
ATGTTGAGTGAAATAAAATGGAAGGGCTTTCTGTTACCGCTAATTTTTGGAATTGGGTTATGGCTAGTTACGCCACTACG
ACCAGTAGCAATTAGTATTATGGCGTGGCACTTATTTGCAATTTTTGTGGCAACGATTATTGCTTGTATTACCAAGCCAT
TACCGATGATGGCCGTCACGCTGATTGGGATTGTTGTCGCGACGTTAACGGGTGTTTTTACGATGGACGAAGTCGCTACT
GGTTTTGGTAATTCGACGGCGTGGATGGTTGCCATGTGCATGTTCATGGCTGCTGGTTTTATTAAATCAGGACTTGGCAA
GCGGATCGCCTATCTGTTTGTCAGTTTATTTGGCAAGCGCACGCTGAGCTTAGCATACGCCTTATCCTTTGTTGATGCCG
TATTGGCGATTGGTATTCCTAGCAATAATGCGCGGGTCAACGGGATTATGTATCCCATTATTGATAATCTTTCTCGTGAA
ATGGGATCTGATCCGAAACAGGGCACACAGCGAAAATTAGGCTCATTTCTAGTTTTTAATGAATATGAAGTTAATAATAT
TACATCAGGCTTGTTTTTGACCGGTTTAGCTGGCAACATGGTGGCTATTGGTTTAGCTAAGATGCAGGGAATCACGGTAA
CTTGGATGCAATGGTTTATGGCTGCTTGTGTCCCAGGTATTTTATCGCTACTAATTATTCCATGGGTACTATATAAAGTC
TATCCCCCTGAGGTGAAACAAACGCCAGACGCGCACCGCTGGGCAACGACGCGTTTGGCCGCACTTGGGAAAATGACAGC
TAGTGAAAAAATTATGTCAATTACCTTTATTTTAGCGATTGTTTTATGGTTGATCGGGACTAAAATTGGCATCGATGCGA
CCGAAGTAAGTTTTATTGCAGTTGTTATTTTGCTGATTACGGGCGTGTTAACGACTAACGATTTGCTCAATGAACATTTT
GCTTGGAATATTTTAGTCTGGTTATCGATCATCATGATGATGTCTAAGAAGCTAATGACACTAGGCTTTTTCCCATGGTT
TTCCAAAACGTTAGGTCATTTACTGAATGGTATGAACTGGATTAGCGTGCTCATTATCCTGTTTTTAGCTTATTTTTACT
TACATTACATGTTTCCGAGTGTTTCAACACAGATTTCGGCGTTATATGCTGGCTTTTTATCCATCGCCATTGGTGCCGGT
GTTCCCCATGTTTTAGCGGCGTTACTATTGGCATTTGCTGGTTCAATCAACTTGTCAACGACGCCGTACTCGGCCGGGCC
CGCGGCATTATTGTCAAGTACAGGCTATGTGACTAGTGCGGAGTGGTGGCGATTGAGTGCAATTATTGGGGTTATTTTTA
ACGTCATCTGGTTAGGTGGGGGCTTGCTGTGGACCAAGTTATTAGGCTTTTGGTAA

Upstream 100 bases:

>100_bases
ATAAATTAAGTTATGGTGATATTGAAAAACGGCAAACAACCGTTAATGGCAAGATATGTGAAAATCTTAACAATTATATG
AAGATTAGGAGGAAGTGCAG

Downstream 100 bases:

>100_bases
GACTAACTGAGCAATATAGATGCGGTAAGTCGTGATTGATCCTGACCACGATTTGCCGCATCTTTCGTGTTAAAATTAGG
TATTAAATAGATTGAATGGG

Product: cation transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLSEIKWKGFLLPLIFGIGLWLVTPLRPVAISIMAWHLFAIFVATIIACITKPLPMMAVTLIGIVVATLTGVFTMDEVAT
GFGNSTAWMVAMCMFMAAGFIKSGLGKRIAYLFVSLFGKRTLSLAYALSFVDAVLAIGIPSNNARVNGIMYPIIDNLSRE
MGSDPKQGTQRKLGSFLVFNEYEVNNITSGLFLTGLAGNMVAIGLAKMQGITVTWMQWFMAACVPGILSLLIIPWVLYKV
YPPEVKQTPDAHRWATTRLAALGKMTASEKIMSITFILAIVLWLIGTKIGIDATEVSFIAVVILLITGVLTTNDLLNEHF
AWNILVWLSIIMMMSKKLMTLGFFPWFSKTLGHLLNGMNWISVLIILFLAYFYLHYMFPSVSTQISALYAGFLSIAIGAG
VPHVLAALLLAFAGSINLSTTPYSAGPAALLSSTGYVTSAEWWRLSAIIGVIFNVIWLGGGLLWTKLLGFW

Sequences:

>Translated_471_residues
MLSEIKWKGFLLPLIFGIGLWLVTPLRPVAISIMAWHLFAIFVATIIACITKPLPMMAVTLIGIVVATLTGVFTMDEVAT
GFGNSTAWMVAMCMFMAAGFIKSGLGKRIAYLFVSLFGKRTLSLAYALSFVDAVLAIGIPSNNARVNGIMYPIIDNLSRE
MGSDPKQGTQRKLGSFLVFNEYEVNNITSGLFLTGLAGNMVAIGLAKMQGITVTWMQWFMAACVPGILSLLIIPWVLYKV
YPPEVKQTPDAHRWATTRLAALGKMTASEKIMSITFILAIVLWLIGTKIGIDATEVSFIAVVILLITGVLTTNDLLNEHF
AWNILVWLSIIMMMSKKLMTLGFFPWFSKTLGHLLNGMNWISVLIILFLAYFYLHYMFPSVSTQISALYAGFLSIAIGAG
VPHVLAALLLAFAGSINLSTTPYSAGPAALLSSTGYVTSAEWWRLSAIIGVIFNVIWLGGGLLWTKLLGFW
>Mature_471_residues
MLSEIKWKGFLLPLIFGIGLWLVTPLRPVAISIMAWHLFAIFVATIIACITKPLPMMAVTLIGIVVATLTGVFTMDEVAT
GFGNSTAWMVAMCMFMAAGFIKSGLGKRIAYLFVSLFGKRTLSLAYALSFVDAVLAIGIPSNNARVNGIMYPIIDNLSRE
MGSDPKQGTQRKLGSFLVFNEYEVNNITSGLFLTGLAGNMVAIGLAKMQGITVTWMQWFMAACVPGILSLLIIPWVLYKV
YPPEVKQTPDAHRWATTRLAALGKMTASEKIMSITFILAIVLWLIGTKIGIDATEVSFIAVVILLITGVLTTNDLLNEHF
AWNILVWLSIIMMMSKKLMTLGFFPWFSKTLGHLLNGMNWISVLIILFLAYFYLHYMFPSVSTQISALYAGFLSIAIGAG
VPHVLAALLLAFAGSINLSTTPYSAGPAALLSSTGYVTSAEWWRLSAIIGVIFNVIWLGGGLLWTKLLGFW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=467, Percent_Identity=33.1905781584582, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1786986, Length=453, Percent_Identity=33.3333333333333, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789444, Length=486, Percent_Identity=33.5390946502058, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 51497; Mature: 51497

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MLSEIKWKGFLLPLIFGIGLWLVTPLRPVAISIMAWHLFAIFVATIIACITKPLPMMAVT
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LIGIVVATLTGVFTMDEVATGFGNSTAWMVAMCMFMAAGFIKSGLGKRIAYLFVSLFGKR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLSLAYALSFVDAVLAIGIPSNNARVNGIMYPIIDNLSREMGSDPKQGTQRKLGSFLVFN
HHHHHHHHHHHHHHHHHCCCCCCCEECCEEHHHHHHHHHHHCCCCCHHHHHHHCCEEEEE
EYEVNNITSGLFLTGLAGNMVAIGLAKMQGITVTWMQWFMAACVPGILSLLIIPWVLYKV
CEECCHHCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPPEVKQTPDAHRWATTRLAALGKMTASEKIMSITFILAIVLWLIGTKIGIDATEVSFIA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VVILLITGVLTTNDLLNEHFAWNILVWLSIIMMMSKKLMTLGFFPWFSKTLGHLLNGMNW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
ISVLIILFLAYFYLHYMFPSVSTQISALYAGFLSIAIGAGVPHVLAALLLAFAGSINLST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
TPYSAGPAALLSSTGYVTSAEWWRLSAIIGVIFNVIWLGGGLLWTKLLGFW
CCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]