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The map label for this gene is glpF [H]

Identifier: 28211621

GI number: 28211621

Start: 2124330

End: 2125034

Strand: Direct

Name: glpF [H]

Synonym: CTC01996

Alternate gene names: 28211621

Gene position: 2124330-2125034 (Clockwise)

Preceding gene: 28211617

Following gene: 28211622

Centisome position: 75.89

GC content: 37.59

Gene sequence:

>705_bases
ATGTCAGCGTTCATGGGTGAATTGCTTGGAACTATGATTTTAATTCTATTAGGTAATGGTGTTGTAGCTAACAATGTGCT
TAAACACTCAAAAGGTGAAGGATCTGGATGGATTGTAATAACAACCGGATGGGCTATTGCAGTTGCTATTCCAGTATATA
TATTTGGACCAATAAGTGGAGCACATTTTAATCCAGCTGTAACTATTGCATTTGCATCCATTGGTCAATTCTCTTGGGCA
GACGTTCCTGGATATATAATAGCTCAATTAGTAGGAGCTATTATTGGAGCAACTTTAGTATGGCTTACTTATTTACCCCA
TTGGGAAGCAACAGAAGATCAAGCTACTAAACTTGCAGCCTTTTCTACAGCACCAGCTATAAAAAACACTACAGCAAATT
TAATTACTGAAATTATAGGAACATTCTTCTTAGTATTTGGTATACTAGGAGTTGCAAATACCAGTATGGCAGATGGACTA
GCTCCTCTAGTTGTTGGTTTAATAATTCTTGCTGCAGGAGTATCCTTAGGTGGACCTACTGGATATGCTATAAACCCTGC
AAGAGATCTTGGTCCACGTATAGCACATGCTATATTGCCTATAAAAGGTAAAGGCGATTCAGATTGGGGATATGCCTGGA
TACCAATTGTAGGTCCAATAATCGGTGGTCTTATAGGAGCAATTTTCTTTAATATAATGTTTTAA

Upstream 100 bases:

>100_bases
TTGGAGGTGAATTTACTATGGACAAGGTGTAATCGATTAAACTAAGATTTAACCAATTTTACTATGTAAATTAAATATTT
ATTATGGGGAAGAGATTTAA

Downstream 100 bases:

>100_bases
TAATATATAAAAAAAAGAGCAATTGGATACTTTTCCATTGCTCTTTCTTATATTTAATCATTAATAGGATAAAATTACTT
TTCTTAGGAAAATATATAAT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSAFMGELLGTMILILLGNGVVANNVLKHSKGEGSGWIVITTGWAIAVAIPVYIFGPISGAHFNPAVTIAFASIGQFSWA
DVPGYIIAQLVGAIIGATLVWLTYLPHWEATEDQATKLAAFSTAPAIKNTTANLITEIIGTFFLVFGILGVANTSMADGL
APLVVGLIILAAGVSLGGPTGYAINPARDLGPRIAHAILPIKGKGDSDWGYAWIPIVGPIIGGLIGAIFFNIMF

Sequences:

>Translated_234_residues
MSAFMGELLGTMILILLGNGVVANNVLKHSKGEGSGWIVITTGWAIAVAIPVYIFGPISGAHFNPAVTIAFASIGQFSWA
DVPGYIIAQLVGAIIGATLVWLTYLPHWEATEDQATKLAAFSTAPAIKNTTANLITEIIGTFFLVFGILGVANTSMADGL
APLVVGLIILAAGVSLGGPTGYAINPARDLGPRIAHAILPIKGKGDSDWGYAWIPIVGPIIGGLIGAIFFNIMF
>Mature_233_residues
SAFMGELLGTMILILLGNGVVANNVLKHSKGEGSGWIVITTGWAIAVAIPVYIFGPISGAHFNPAVTIAFASIGQFSWAD
VPGYIIAQLVGAIIGATLVWLTYLPHWEATEDQATKLAAFSTAPAIKNTTANLITEIIGTFFLVFGILGVANTSMADGLA
PLVVGLIILAAGVSLGGPTGYAINPARDLGPRIAHAILPIKGKGDSDWGYAWIPIVGPIIGGLIGAIFFNIMF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=241, Percent_Identity=35.2697095435685, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=34.5381526104418, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI22538420, Length=248, Percent_Identity=31.4516129032258, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI4502187, Length=245, Percent_Identity=31.0204081632653, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=33.495145631068, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502181, Length=206, Percent_Identity=33.495145631068, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310133356, Length=216, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI310114181, Length=216, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI37694062, Length=201, Percent_Identity=31.3432835820896, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI45446752, Length=212, Percent_Identity=29.2452830188679, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI4502179, Length=242, Percent_Identity=29.3388429752066, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1790362, Length=248, Percent_Identity=34.2741935483871, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=241, Percent_Identity=33.195020746888, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531431, Length=236, Percent_Identity=31.7796610169492, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531429, Length=236, Percent_Identity=31.7796610169492, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17544068, Length=244, Percent_Identity=33.6065573770492, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71992966, Length=244, Percent_Identity=30.7377049180328, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17533613, Length=237, Percent_Identity=29.535864978903, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI32564052, Length=237, Percent_Identity=29.535864978903, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=188, Percent_Identity=33.5106382978723, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71993722, Length=237, Percent_Identity=30.8016877637131, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=231, Percent_Identity=30.7359307359307, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=34.4660194174757, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322985, Length=284, Percent_Identity=26.056338028169, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652747, Length=234, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45551084, Length=234, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24762342, Length=244, Percent_Identity=29.5081967213115, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550503, Length=234, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24256; Mature: 24125

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFMGELLGTMILILLGNGVVANNVLKHSKGEGSGWIVITTGWAIAVAIPVYIFGPISG
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCC
AHFNPAVTIAFASIGQFSWADVPGYIIAQLVGAIIGATLVWLTYLPHWEATEDQATKLAA
CCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FSTAPAIKNTTANLITEIIGTFFLVFGILGVANTSMADGLAPLVVGLIILAAGVSLGGPT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCCC
GYAINPARDLGPRIAHAILPIKGKGDSDWGYAWIPIVGPIIGGLIGAIFFNIMF
CCCCCCHHHHHHHHHHHHEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAFMGELLGTMILILLGNGVVANNVLKHSKGEGSGWIVITTGWAIAVAIPVYIFGPISG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCC
AHFNPAVTIAFASIGQFSWADVPGYIIAQLVGAIIGATLVWLTYLPHWEATEDQATKLAA
CCCCCCEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FSTAPAIKNTTANLITEIIGTFFLVFGILGVANTSMADGLAPLVVGLIILAAGVSLGGPT
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCCCCC
GYAINPARDLGPRIAHAILPIKGKGDSDWGYAWIPIVGPIIGGLIGAIFFNIMF
CCCCCCHHHHHHHHHHHHEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]