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The map label for this gene is insK [H]

Identifier: 28211615

GI number: 28211615

Start: 2115510

End: 2116430

Strand: Direct

Name: insK [H]

Synonym: CTC01990

Alternate gene names: 28211615

Gene position: 2115510-2116430 (Clockwise)

Preceding gene: 28211614

Following gene: 28211617

Centisome position: 75.57

GC content: 27.9

Gene sequence:

>921_bases
GTGAAATTGCTAACGCAACAAAAATATATCCTTATAAAATCAGTTATAGATAAATATAAACTTAAAAATTTAGTTAGTTA
CTTATGTAAACTATCTAATGTTTCACGTTCAGGATATTATAATTACTTTTCTATAAAATCTCATACTAATCGTATATCAC
GCGAAGAGCGTGATTTAGAAAGCTATAATAATATATTAATTGCATACAACTTCAAAAAGCGTAAAAAAGGTGCTAGACAA
ATTAAAATGACTTTAGAAAATCAGTTTCATATTAATTATAACCTAAAGCGTATTAGAAGAATAATGCATAAATATGATAT
TATATGTCCGCATAGAAAAGCTAACCCTTATAGAAGAATGATGAAGGCTAACAAAGAACATTCTGTCTTGCCAAATTTAT
TAAATAGAGAATTTAAACAAGATGTACCTGGAAAGGTATTGCTCACTGACATAACATATCTATTTTATAAAAATGGTCAA
AGGGCTTATTTGTCAACCGTTTTAGATGGTTCAACCAATGAATTATTAACCTATAATCTATCTAAAAGTCTAAAGATAGA
TATTGTGACTGAAACTATTGAAAAATTGGTTTCATCAAATAACTATTTAATATCGTCAGATTCATTTATTCATTCAGATC
AAGGGGCACATTACACTAGTCCCATATTTCAGAAATTGCTTAAAAAATATAATATAAAACAATCTATGTCTAGACGAGGT
AACTGCTGGGATAATGCTCCACAGGAGTCATTCTTTGGACATCTTAAGGATGAAACAAATATTAAAAATTGTAATACATT
TGCTGAACTCTTGGAAGAAATTGATGAATATATGGATTATTATAATAATTATAGAGGTCAATGGAATTTAAAAAAGATGA
CTCCTGTAAAATACAGAAATCATCTTCTTAGTACCGCCTAG

Upstream 100 bases:

>100_bases
AAAAGTATGAAAAACTTCAGACTAAAATAAAACTCTTAGAAGCAGAAAATGAACTGCTAAAAAAGTTAGAAAGGTTGGAA
AGGAGCGTGAAAAAGAAAAA

Downstream 100 bases:

>100_bases
TCTTTTTTTAAACTGTCCTTGACAAAGGGTACATTTTATAGAATATTACTATGTTTGGCTCTTTTTTATAATATTTTTAT
TTGGCTGGTATTATTAATCT

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLESYNNILIAYNFKKRKKGARQ
IKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQ
RAYLSTVLDGSTNELLTYNLSKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRG
NCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRNHLLSTA

Sequences:

>Translated_306_residues
MKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLESYNNILIAYNFKKRKKGARQ
IKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQ
RAYLSTVLDGSTNELLTYNLSKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRG
NCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRNHLLSTA
>Mature_306_residues
MKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLESYNNILIAYNFKKRKKGARQ
IKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRMMKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQ
RAYLSTVLDGSTNELLTYNLSKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRG
NCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRNHLLSTA

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=276, Percent_Identity=31.5217391304348, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788405, Length=248, Percent_Identity=26.2096774193548, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787263, Length=248, Percent_Identity=26.2096774193548, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786753, Length=248, Percent_Identity=26.2096774193548, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786570, Length=248, Percent_Identity=26.2096774193548, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786490, Length=248, Percent_Identity=26.2096774193548, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 36359; Mature: 36359

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHH
SYNNILIAYNFKKRKKGARQIKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRM
HHCCEEEEEECHHHHCHHHHHHHEECCEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHH
MKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQRAYLSTVLDGSTNELLTYNL
HHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEC
SKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRG
CCCEEEEHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHCHHHHHHHCC
NCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRN
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHH
HLLSTA
HHHCCC
>Mature Secondary Structure
MKLLTQQKYILIKSVIDKYKLKNLVSYLCKLSNVSRSGYYNYFSIKSHTNRISREERDLE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHH
SYNNILIAYNFKKRKKGARQIKMTLENQFHINYNLKRIRRIMHKYDIICPHRKANPYRRM
HHCCEEEEEECHHHHCHHHHHHHEECCEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHH
MKANKEHSVLPNLLNREFKQDVPGKVLLTDITYLFYKNGQRAYLSTVLDGSTNELLTYNL
HHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEC
SKSLKIDIVTETIEKLVSSNNYLISSDSFIHSDQGAHYTSPIFQKLLKKYNIKQSMSRRG
CCCEEEEHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCHHHHHHHHHHCHHHHHHHCC
NCWDNAPQESFFGHLKDETNIKNCNTFAELLEEIDEYMDYYNNYRGQWNLKKMTPVKYRN
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHH
HLLSTA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]