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The map label for this gene is truA

Identifier: 28211488

GI number: 28211488

Start: 1967301

End: 1968038

Strand: Direct

Name: truA

Synonym: CTC01851

Alternate gene names: 28211488

Gene position: 1967301-1968038 (Clockwise)

Preceding gene: 28211486

Following gene: 28211491

Centisome position: 70.28

GC content: 28.46

Gene sequence:

>738_bases
ATGAGAAATTTAAAGATGATATTAGAATACGACGGAACTAGATATAAAGGATGGCAAAAACAAAAGAAAGATGTTTTAAC
TATACAAGATAAAATAGAAACTGTATTATCTAAAATGACTGGAGAAGATATTCAAGTAATAGGTTGTGGAAGAACCGACG
CAGGCGTTCACGCCGAAAATTATGTAGCAAACTTTCATACTAATTGTGATTTTACTGTGGATTATATGTTAGATTATCTA
TATGAATTTTTACCTGAAGATATAGTGGTAAAAACTATGAAGGATACCAGTGAAAGATTCCATGCAAGATATAATGTAAA
GTCAAAAACTTATGTTTATAGAATAAATAATAATAAATTTAGAAGTGTATTTAATAAAAAGTATGCTTATCATGACAATG
AGAAACTTAATATAAGTGCAATGAAAGATGCTGCAGAATTTTTAATTGGAACTCATGACTTTAAAAGCTTTACTCGATTA
AAATCTAATTCTAAATCAACTATTAGAGCTATTAAATACATAAACATAACAGAAAATCAAGGTATAATAAGTATAGAAGT
TAATGGAAATGGCTTCTTGTTAAATATGGTAAGAATCGTTGCAGGGGCTCTTTTAGAGGTGGGTAAGGAAAATATTAAAC
CCATAGATATTGAAAAAATGTTAAATGAGAAGAAAAGAGCAGATGCTAGTCTAATCCTTCCTGCAAAGGGATTATGCTTA
AAGGATATTCAATATTAA

Upstream 100 bases:

>100_bases
TTTTCAATTCTTTAATTATATCATAAATAAAATATCTATAAATAATAGACTTTATAGTATATAATTAAAGAATCATTAAA
AACATTAAAGGTGGGTCTAA

Downstream 100 bases:

>100_bases
TAGTAAAAATAAACTGTAAACACCTTTGTTTTGTTTACAGTTTATTTTATTAATCCAAAAATATTCTATTTATTCATATA
GTCCAAAACCAGTTATAACT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRNLKMILEYDGTRYKGWQKQKKDVLTIQDKIETVLSKMTGEDIQVIGCGRTDAGVHAENYVANFHTNCDFTVDYMLDYL
YEFLPEDIVVKTMKDTSERFHARYNVKSKTYVYRINNNKFRSVFNKKYAYHDNEKLNISAMKDAAEFLIGTHDFKSFTRL
KSNSKSTIRAIKYINITENQGIISIEVNGNGFLLNMVRIVAGALLEVGKENIKPIDIEKMLNEKKRADASLILPAKGLCL
KDIQY

Sequences:

>Translated_245_residues
MRNLKMILEYDGTRYKGWQKQKKDVLTIQDKIETVLSKMTGEDIQVIGCGRTDAGVHAENYVANFHTNCDFTVDYMLDYL
YEFLPEDIVVKTMKDTSERFHARYNVKSKTYVYRINNNKFRSVFNKKYAYHDNEKLNISAMKDAAEFLIGTHDFKSFTRL
KSNSKSTIRAIKYINITENQGIISIEVNGNGFLLNMVRIVAGALLEVGKENIKPIDIEKMLNEKKRADASLILPAKGLCL
KDIQY
>Mature_245_residues
MRNLKMILEYDGTRYKGWQKQKKDVLTIQDKIETVLSKMTGEDIQVIGCGRTDAGVHAENYVANFHTNCDFTVDYMLDYL
YEFLPEDIVVKTMKDTSERFHARYNVKSKTYVYRINNNKFRSVFNKKYAYHDNEKLNISAMKDAAEFLIGTHDFKSFTRL
KSNSKSTIRAIKYINITENQGIISIEVNGNGFLLNMVRIVAGALLEVGKENIKPIDIEKMLNEKKRADASLILPAKGLCL
KDIQY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=268, Percent_Identity=25, Blast_Score=91, Evalue=6e-19,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=34.0080971659919, Blast_Score=153, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI71985046, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321112, Length=234, Percent_Identity=26.4957264957265, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116007752, Length=294, Percent_Identity=25.5102040816327, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA1_CLOTE (Q893H3)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_782432.1
- ProteinModelPortal:   Q893H3
- SMR:   Q893H3
- GeneID:   1060994
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01851
- NMPDR:   fig|212717.1.peg.1654
- HOGENOM:   HBG621020
- OMA:   NYCYEYL
- PhylomeDB:   Q893H3
- ProtClustDB:   PRK12434
- BioCyc:   CTET212717:CTC_01851-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28264; Mature: 28264

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLKMILEYDGTRYKGWQKQKKDVLTIQDKIETVLSKMTGEDIQVIGCGRTDAGVHAEN
CCCEEEEEEECCCCCCCCHHHCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
YVANFHTNCDFTVDYMLDYLYEFLPEDIVVKTMKDTSERFHARYNVKSKTYVYRINNNKF
EEEEECCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCHH
RSVFNKKYAYHDNEKLNISAMKDAAEFLIGTHDFKSFTRLKSNSKSTIRAIKYINITENQ
HHHHHHHCEECCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEEEECCC
GIISIEVNGNGFLLNMVRIVAGALLEVGKENIKPIDIEKMLNEKKRADASLILPAKGLCL
CEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCC
KDIQY
CCCCC
>Mature Secondary Structure
MRNLKMILEYDGTRYKGWQKQKKDVLTIQDKIETVLSKMTGEDIQVIGCGRTDAGVHAEN
CCCEEEEEEECCCCCCCCHHHCCCEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHH
YVANFHTNCDFTVDYMLDYLYEFLPEDIVVKTMKDTSERFHARYNVKSKTYVYRINNNKF
EEEEECCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCHH
RSVFNKKYAYHDNEKLNISAMKDAAEFLIGTHDFKSFTRLKSNSKSTIRAIKYINITENQ
HHHHHHHCEECCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEEEECCC
GIISIEVNGNGFLLNMVRIVAGALLEVGKENIKPIDIEKMLNEKKRADASLILPAKGLCL
CEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCC
KDIQY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129