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The map label for this gene is gluD [H]

Identifier: 28210980

GI number: 28210980

Start: 1391585

End: 1392850

Strand: Direct

Name: gluD [H]

Synonym: CTC01295

Alternate gene names: 28210980

Gene position: 1391585-1392850 (Clockwise)

Preceding gene: 28210979

Following gene: 28210981

Centisome position: 49.71

GC content: 31.28

Gene sequence:

>1266_bases
ATGAATGTAGAAGAATTAAATCCGTTTAAAAATGTGCAAAAAATAATGAAGAAAATCTGCGAGGATCTTAATTTAAATGA
AAGTGTTTATGAATTAATTAAGGAACCTTCAAGGATAGTTGAAGTTAATATCCCAGTTAAAATGGATAATGGTTCTATAA
AAGTTTTTAAGGGATATAGAAGTCAACATAATAATTCCATAGGTGCTACAAAGGGTGGAGTTAGATTTCATCAAGGTGTA
AATTTAGATGAAGTAAAAGCTTTATCTATATGGATGACCTTAAAATGTGCTATAGCAAATCTTCCTTTTGGAGGGGGAAA
GGGTGGAATTATAGTAGATCCTAAAACTTTATCCAAAGGTGAATTAGAAAGATTAAGTAGGGGATATGTAGAAAAACTTT
ATGAGGTTTTAGGTGAAGATTTAGATATTCCAGCTCCAGATGTAAATACAAATGGTGAAATAATTGCTTGGATGGCAGAC
GAATATAATAAGCTTTCAAGACAAAATGCTTGGGGAACTTTCACAGGTAAACCAGTAGAATTAAATGGTTCTAAGGGTAG
AACTGAAGCAACAGGTTTAGGGGTAGCTATAGTTGCTAGAGAAGCTTTAAAAAAACTTAATAGAAATATAGAAAATTCTT
CTGTAGCGGTTCAAGGCTTTGGAAATGTAGGAAGTCACGCTGCTCTTTGCATAGAAAACCTAGGTGGGAAAATAGTTAGT
GTATCAGAATGGGATAGAGAAAAAGGATTTTATGCCATATATGATGAAAAAGGATTAAACATTAAAGAATTAATAAGTTA
CTTTAATGAAAATGGAACTTTACTTGACTTCCCTAGAACTTCTAAAATAAGCGAAGAAGAATTTTGGAGTTTATCAGTGG
ATGTGTTAGTTCCGGCAGCATTAGAAAATTCCATAAATACAAATAATGCTAATTTAATAAATGCTAAACTAGTTTGTGAA
GGAGCTAATGGACCTGTTACACCTGCAGCGGATGAAATATTAGAGAAAAAAGGAATTGAAGTTACACCAGATATATTAAC
AAATGCAGGTGGAGTTATAGTTTCTTATTTTGAATGGGTACAAAATTTAGATAATTACTATTGGAATGAAAAAGATGTTA
AAACTCGTGAAGAAGAATTCTTAGTAGAAGGATTTAACAATGTGTGGTTTATGAAAGATAAATTTAATTGTACTATGAGA
AATGCAGCTTATATGTATGCTATAAATAAATTAGCGAAATCAATGAAAGCTAGAGGATGGTATTAA

Upstream 100 bases:

>100_bases
TATATAAATAAAAAATGAAAACTTTACAGAAAATACAAGAACTTTGTAAAAAAATGTAGTATAATATACATATATGCTAC
TAAATTAGGGGGTTAAAATA

Downstream 100 bases:

>100_bases
AACTTTAAAAAAATAAGGTAAATAAAGTAGAAGTTAAAAACTTCTACTTTATTTAATGATAATATGTATCAATTGATATA
ATAAAAGAGAAAAATATCAA

Product: NAD-specific glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNVEELNPFKNVQKIMKKICEDLNLNESVYELIKEPSRIVEVNIPVKMDNGSIKVFKGYRSQHNNSIGATKGGVRFHQGV
NLDEVKALSIWMTLKCAIANLPFGGGKGGIIVDPKTLSKGELERLSRGYVEKLYEVLGEDLDIPAPDVNTNGEIIAWMAD
EYNKLSRQNAWGTFTGKPVELNGSKGRTEATGLGVAIVAREALKKLNRNIENSSVAVQGFGNVGSHAALCIENLGGKIVS
VSEWDREKGFYAIYDEKGLNIKELISYFNENGTLLDFPRTSKISEEEFWSLSVDVLVPAALENSINTNNANLINAKLVCE
GANGPVTPAADEILEKKGIEVTPDILTNAGGVIVSYFEWVQNLDNYYWNEKDVKTREEEFLVEGFNNVWFMKDKFNCTMR
NAAYMYAINKLAKSMKARGWY

Sequences:

>Translated_421_residues
MNVEELNPFKNVQKIMKKICEDLNLNESVYELIKEPSRIVEVNIPVKMDNGSIKVFKGYRSQHNNSIGATKGGVRFHQGV
NLDEVKALSIWMTLKCAIANLPFGGGKGGIIVDPKTLSKGELERLSRGYVEKLYEVLGEDLDIPAPDVNTNGEIIAWMAD
EYNKLSRQNAWGTFTGKPVELNGSKGRTEATGLGVAIVAREALKKLNRNIENSSVAVQGFGNVGSHAALCIENLGGKIVS
VSEWDREKGFYAIYDEKGLNIKELISYFNENGTLLDFPRTSKISEEEFWSLSVDVLVPAALENSINTNNANLINAKLVCE
GANGPVTPAADEILEKKGIEVTPDILTNAGGVIVSYFEWVQNLDNYYWNEKDVKTREEEFLVEGFNNVWFMKDKFNCTMR
NAAYMYAINKLAKSMKARGWY
>Mature_421_residues
MNVEELNPFKNVQKIMKKICEDLNLNESVYELIKEPSRIVEVNIPVKMDNGSIKVFKGYRSQHNNSIGATKGGVRFHQGV
NLDEVKALSIWMTLKCAIANLPFGGGKGGIIVDPKTLSKGELERLSRGYVEKLYEVLGEDLDIPAPDVNTNGEIIAWMAD
EYNKLSRQNAWGTFTGKPVELNGSKGRTEATGLGVAIVAREALKKLNRNIENSSVAVQGFGNVGSHAALCIENLGGKIVS
VSEWDREKGFYAIYDEKGLNIKELISYFNENGTLLDFPRTSKISEEEFWSLSVDVLVPAALENSINTNNANLINAKLVCE
GANGPVTPAADEILEKKGIEVTPDILTNAGGVIVSYFEWVQNLDNYYWNEKDVKTREEEFLVEGFNNVWFMKDKFNCTMR
NAAYMYAINKLAKSMKARGWY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=380, Percent_Identity=35.7894736842105, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4885281, Length=380, Percent_Identity=35.7894736842105, Blast_Score=252, Evalue=6e-67,
Organism=Escherichia coli, GI1788059, Length=366, Percent_Identity=33.0601092896175, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17544676, Length=396, Percent_Identity=36.3636363636364, Blast_Score=248, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6319256, Length=396, Percent_Identity=31.3131313131313, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324951, Length=389, Percent_Identity=31.1053984575836, Blast_Score=146, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24649283, Length=374, Percent_Identity=37.9679144385027, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI45549226, Length=360, Percent_Identity=37.7777777777778, Blast_Score=247, Evalue=8e-66,
Organism=Drosophila melanogaster, GI45553475, Length=360, Percent_Identity=37.7777777777778, Blast_Score=247, Evalue=9e-66,
Organism=Drosophila melanogaster, GI24649460, Length=300, Percent_Identity=37, Blast_Score=195, Evalue=5e-50,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46903; Mature: 46903

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVEELNPFKNVQKIMKKICEDLNLNESVYELIKEPSRIVEVNIPVKMDNGSIKVFKGYR
CCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEEEECCCCEEEEECCH
SQHNNSIGATKGGVRFHQGVNLDEVKALSIWMTLKCAIANLPFGGGKGGIIVDPKTLSKG
HHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHEEEEHHHCCCCCCCCCCEEECCCCCCHH
ELERLSRGYVEKLYEVLGEDLDIPAPDVNTNGEIIAWMADEYNKLSRQNAWGTFTGKPVE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCCCEEE
LNGSKGRTEATGLGVAIVAREALKKLNRNIENSSVAVQGFGNVGSHAALCIENLGGKIVS
ECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEE
VSEWDREKGFYAIYDEKGLNIKELISYFNENGTLLDFPRTSKISEEEFWSLSVDVLVPAA
ECCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHEEEEHEEEEHH
LENSINTNNANLINAKLVCEGANGPVTPAADEILEKKGIEVTPDILTNAGGVIVSYFEWV
HHCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHCCCEECHHHHHCCCHHHHHHHHHH
QNLDNYYWNEKDVKTREEEFLVEGFNNVWFMKDKFNCTMRNAAYMYAINKLAKSMKARGW
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCC
Y
C
>Mature Secondary Structure
MNVEELNPFKNVQKIMKKICEDLNLNESVYELIKEPSRIVEVNIPVKMDNGSIKVFKGYR
CCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEEEEECCCCEEEEECCH
SQHNNSIGATKGGVRFHQGVNLDEVKALSIWMTLKCAIANLPFGGGKGGIIVDPKTLSKG
HHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHEEEEHHHCCCCCCCCCCEEECCCCCCHH
ELERLSRGYVEKLYEVLGEDLDIPAPDVNTNGEIIAWMADEYNKLSRQNAWGTFTGKPVE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCCCEEE
LNGSKGRTEATGLGVAIVAREALKKLNRNIENSSVAVQGFGNVGSHAALCIENLGGKIVS
ECCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEE
VSEWDREKGFYAIYDEKGLNIKELISYFNENGTLLDFPRTSKISEEEFWSLSVDVLVPAA
ECCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHEEEEHEEEEHH
LENSINTNNANLINAKLVCEGANGPVTPAADEILEKKGIEVTPDILTNAGGVIVSYFEWV
HHCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHCCCEECHHHHHCCCHHHHHHHHHH
QNLDNYYWNEKDVKTREEEFLVEGFNNVWFMKDKFNCTMRNAAYMYAINKLAKSMKARGW
HHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1774279 [H]