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The map label for this gene is rimO

Identifier: 28210972

GI number: 28210972

Start: 1381295

End: 1382629

Strand: Direct

Name: rimO

Synonym: CTC01287

Alternate gene names: 28210972

Gene position: 1381295-1382629 (Clockwise)

Preceding gene: 28210971

Following gene: 28210973

Centisome position: 49.35

GC content: 28.69

Gene sequence:

>1335_bases
ATGACAAAAATAAAATATGGAGTAGTTAGTTTAGGTTGCGATAAAAATAGAATTGACTCTGAGGTAATGATAAATGAGAT
TAAAAAAGAAGGTATAATAACAAATGATCCTAAAGAAGCAGATGTTATTATAGTAAATACTTGTGGATTTATAGAAGATT
CTAAAAAAGAGTCTATAGATACAATATTAGAAATGTCAAACTATAAAAATAATAACTGTAAAGTATTGGTAGTTACTGGT
TGTTTAAGCCAAAGATATGGAGAAGAATTACAAGAATTACTTCCAGAAGTAGATGTAATGTTAGGAGTAAATGACTATGA
TAAGCTTAGTGATGCTATTAAAAAATCCATAGAAAAAGGTGAAAAGTCCCTTTATTGTAACTATAGTAATACAGTTATAA
ATGAAGGTGGTAGGGTATTAACTACACAAAAACATTATGCCTATTTAAGAATAGCTGAAGGTTGCGACAATTTCTGTACT
TATTGTGCAATACCAAAAATAAGGGGAAAATACAGAAGTAGAAAAATTGAGGACATAATAGAAGAAGCTAAATTTCTTTC
GCAAAATGGTGTTAAAGAAATAATAATAGTTGCACAAGATACTACAAGATATGGATTGGACATATATGGTGAAAAAACGC
TACCTTCACTTTTAAAACAATTAGAAGAAGTAGATGGAATAGAATGGATAAGATTATTATATTGTTATCCAGAAGATATA
ACAGAGGAGCTAATAGAAGAGTTTGCCAGAAACAAAAAGTTATGTAAGTATGTAGATGTACCTATACAGCACATAAGTGA
TTCTGTATTAAAAAGAATGGGCAGAAAAGGAAATAAACAATTGGTTACAAAGGTTTTAAGAGATATTAAAAAAAGAGTTC
CAGAAATGTCTATAAGAACTTCATTAATAGTAGGTTTTCCAGGAGAAATGGAGGAAGACTTTAAAGAACTTAAAGACTTT
GTAGAAGAATTCAAATTTCAAAATTTAGGAGTTTTTAAATACTCTCAAGAAGAGGGCACACCTGCGGCAACAATGGAAGA
TCAAGTTTTAGAAGAGATAAAGGAAACAAGAAGAGAAGAATTAATGAAGATGCAACGAGACATAGTTAAAAGTATTAATG
CTGATAAAGTTAATAAAGTATACAAAGTAGTTGTAGATAATTTTAATGGAGAACATTATATAGGAAGAAATTATGAAATG
CTACCGGAAATAGATGGTGCAATTTATTTTAAATGTGATAAAATATTAAATATAGGTGAGATGGTATGTATCAAAATATT
AGAGACCTTAGAATATGATCTAATAGGAGTTGTATGTGATGAATCTTGCAAATAA

Upstream 100 bases:

>100_bases
TGGAGGAGAGGGGTATTATATCCCAAAAAGATGGAAGTAAGCCAAGACAGGTATTAATAAGCAAAGATGATATTGTCTAA
GAAGTTTAAGGAGGAGTAAA

Downstream 100 bases:

>100_bases
ATTAACTATAATCAGAATATTTTTAGTACCTGTTTTTTTGATTTTTATAGCCATTAATGACATTCCATATGGAAAAACTA
TAGCTACAGCCATATTTATA

Product: Fe-S oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTKIKYGVVSLGCDKNRIDSEVMINEIKKEGIITNDPKEADVIIVNTCGFIEDSKKESIDTILEMSNYKNNNCKVLVVTG
CLSQRYGEELQELLPEVDVMLGVNDYDKLSDAIKKSIEKGEKSLYCNYSNTVINEGGRVLTTQKHYAYLRIAEGCDNFCT
YCAIPKIRGKYRSRKIEDIIEEAKFLSQNGVKEIIIVAQDTTRYGLDIYGEKTLPSLLKQLEEVDGIEWIRLLYCYPEDI
TEELIEEFARNKKLCKYVDVPIQHISDSVLKRMGRKGNKQLVTKVLRDIKKRVPEMSIRTSLIVGFPGEMEEDFKELKDF
VEEFKFQNLGVFKYSQEEGTPAATMEDQVLEEIKETRREELMKMQRDIVKSINADKVNKVYKVVVDNFNGEHYIGRNYEM
LPEIDGAIYFKCDKILNIGEMVCIKILETLEYDLIGVVCDESCK

Sequences:

>Translated_444_residues
MTKIKYGVVSLGCDKNRIDSEVMINEIKKEGIITNDPKEADVIIVNTCGFIEDSKKESIDTILEMSNYKNNNCKVLVVTG
CLSQRYGEELQELLPEVDVMLGVNDYDKLSDAIKKSIEKGEKSLYCNYSNTVINEGGRVLTTQKHYAYLRIAEGCDNFCT
YCAIPKIRGKYRSRKIEDIIEEAKFLSQNGVKEIIIVAQDTTRYGLDIYGEKTLPSLLKQLEEVDGIEWIRLLYCYPEDI
TEELIEEFARNKKLCKYVDVPIQHISDSVLKRMGRKGNKQLVTKVLRDIKKRVPEMSIRTSLIVGFPGEMEEDFKELKDF
VEEFKFQNLGVFKYSQEEGTPAATMEDQVLEEIKETRREELMKMQRDIVKSINADKVNKVYKVVVDNFNGEHYIGRNYEM
LPEIDGAIYFKCDKILNIGEMVCIKILETLEYDLIGVVCDESCK
>Mature_443_residues
TKIKYGVVSLGCDKNRIDSEVMINEIKKEGIITNDPKEADVIIVNTCGFIEDSKKESIDTILEMSNYKNNNCKVLVVTGC
LSQRYGEELQELLPEVDVMLGVNDYDKLSDAIKKSIEKGEKSLYCNYSNTVINEGGRVLTTQKHYAYLRIAEGCDNFCTY
CAIPKIRGKYRSRKIEDIIEEAKFLSQNGVKEIIIVAQDTTRYGLDIYGEKTLPSLLKQLEEVDGIEWIRLLYCYPEDIT
EELIEEFARNKKLCKYVDVPIQHISDSVLKRMGRKGNKQLVTKVLRDIKKRVPEMSIRTSLIVGFPGEMEEDFKELKDFV
EEFKFQNLGVFKYSQEEGTPAATMEDQVLEEIKETRREELMKMQRDIVKSINADKVNKVYKVVVDNFNGEHYIGRNYEML
PEIDGAIYFKCDKILNIGEMVCIKILETLEYDLIGVVCDESCK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=426, Percent_Identity=27.6995305164319, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI28872784, Length=426, Percent_Identity=27.6995305164319, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=30.8068459657702, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=38.3747178329571, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1786882, Length=364, Percent_Identity=29.9450549450549, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=404, Percent_Identity=27.2277227722772, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71996771, Length=327, Percent_Identity=28.7461773700306, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI21356207, Length=460, Percent_Identity=25.6521739130435, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922432, Length=455, Percent_Identity=29.6703296703297, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_CLOTE (Q895I7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781916.1
- ProteinModelPortal:   Q895I7
- GeneID:   1059537
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01287
- NMPDR:   fig|212717.1.peg.1138
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q895I7
- ProtClustDB:   CLSK882944
- BioCyc:   CTET212717:CTC_01287-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51083; Mature: 50951

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKYGVVSLGCDKNRIDSEVMINEIKKEGIITNDPKEADVIIVNTCGFIEDSKKESID
CCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
TILEMSNYKNNNCKVLVVTGCLSQRYGEELQELLPEVDVMLGVNDYDKLSDAIKKSIEKG
HHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCHHHEEECCCCHHHHHHHHHHHHHCC
EKSLYCNYSNTVINEGGRVLTTQKHYAYLRIAEGCDNFCTYCAIPKIRGKYRSRKIEDII
CCEEEEECCCCEECCCCCEEEECCCEEEEEEHHCHHHHHHHHCCHHHHHHHHHHHHHHHH
EEAKFLSQNGVKEIIIVAQDTTRYGLDIYGEKTLPSLLKQLEEVDGIEWIRLLYCYPEDI
HHHHHHHHCCCEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHCCCHHEEHHEEECCHHH
TEELIEEFARNKKLCKYVDVPIQHISDSVLKRMGRKGNKQLVTKVLRDIKKRVPEMSIRT
HHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHE
SLIVGFPGEMEEDFKELKDFVEEFKFQNLGVFKYSQEEGTPAATMEDQVLEEIKETRREE
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
LMKMQRDIVKSINADKVNKVYKVVVDNFNGEHYIGRNYEMLPEIDGAIYFKCDKILNIGE
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEHHHHCHHH
MVCIKILETLEYDLIGVVCDESCK
HHHHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
TKIKYGVVSLGCDKNRIDSEVMINEIKKEGIITNDPKEADVIIVNTCGFIEDSKKESID
CCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
TILEMSNYKNNNCKVLVVTGCLSQRYGEELQELLPEVDVMLGVNDYDKLSDAIKKSIEKG
HHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCHHHEEECCCCHHHHHHHHHHHHHCC
EKSLYCNYSNTVINEGGRVLTTQKHYAYLRIAEGCDNFCTYCAIPKIRGKYRSRKIEDII
CCEEEEECCCCEECCCCCEEEECCCEEEEEEHHCHHHHHHHHCCHHHHHHHHHHHHHHHH
EEAKFLSQNGVKEIIIVAQDTTRYGLDIYGEKTLPSLLKQLEEVDGIEWIRLLYCYPEDI
HHHHHHHHCCCEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHCCCHHEEHHEEECCHHH
TEELIEEFARNKKLCKYVDVPIQHISDSVLKRMGRKGNKQLVTKVLRDIKKRVPEMSIRT
HHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHE
SLIVGFPGEMEEDFKELKDFVEEFKFQNLGVFKYSQEEGTPAATMEDQVLEEIKETRREE
EEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
LMKMQRDIVKSINADKVNKVYKVVVDNFNGEHYIGRNYEMLPEIDGAIYFKCDKILNIGE
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEHHHHCHHH
MVCIKILETLEYDLIGVVCDESCK
HHHHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129