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The map label for this gene is infB

Identifier: 28210961

GI number: 28210961

Start: 1367297

End: 1369354

Strand: Direct

Name: infB

Synonym: CTC01275

Alternate gene names: 28210961

Gene position: 1367297-1369354 (Clockwise)

Preceding gene: 28210960

Following gene: 28210962

Centisome position: 48.85

GC content: 32.65

Gene sequence:

>2058_bases
GTGAATGCTTTGTCAAAAGTAAGAGTATATGAATTATCAAAAGAATTAGGTATATCAAGTAAAGACTTAATTAGTTTATT
AAAAGATGAATTTAGCATTGAAGTGAAAAATCATATGAGTGTTGTAGAGGAAGAAGATGCTCTATTGATAAAAGAATTTT
ATGGTGAGAGCAAAGAAGAAAATACTGAGGAAAACATAGAAGAAAAATATGGAGACCTTCAAGAAGCTCAAATAAAAGTA
GTAAAGAAAAATAGAAAAAATAAAGTTAAAAAAAATGACGATGAAAATGATGCACAAGAAGAAATAGTAATAGAAATGGA
AGATACCATAACAGTTAAAGAGTTATCAGATAGATTGAAAAAAACTTATGCTGAAGTTATAAAAGAACTAATGATGATGG
GAGTAATGGCTGCAGTTAATCAAGAGATAGATTTTGAAGCAGCAGAAAAGCTTGGTGAAAAATTTGAAGCTATAGTTGTA
CAAAGAGAAGTAGACGTACTAGAAGAGTCTGTGGAACAATACATTGAAGAAGAGGAAGAAGAAGGGACTGTAAAAAGACC
TCCAGTAGTTACAGTTATGGGTCACGTTGACCATGGTAAAACTTCATTATTAGATGCTATTAGAAAAGAAGAAGTAGCTG
CTAGTGAAGCAGGAGGTATAACTCAACATATAGGAGCCTACACTATAACTATAAATGGTGAAAAAATAACATTCTTAGAT
ACGCCAGGGCATGAAGCTTTTACATCAATGAGAGCTAGAGGTGCTCAGATAACAGATATAGTTATATTAGTTGTTGCAGC
AGATGATGGTATAATGCCACAAACTGAAGAAGCTATTAATCACTGTAAAGCTGCGAATGTTCCAATGGTAGTTGCTATAA
ATAAAATGGATAGAGCTGGTGCTAATCCAGATAAAGTAAAGCAACAATTAGCAGAAAAAGGCCTTGTAGCAGAAGACTGG
GGTGGAGATACTATTACTGTGCCAGTATCAGCTCATACAAAGGAAGGCATAGATACATTATTAGAAATGGTACTTTTAAC
AGCAGAAATGCAAGAGTTAAAGTCAAATCCTAATAGAAAAGCTAAGGGAACAGTTGTAGATGCTAAATTAGATAAGGGAA
GAGGACCTGTGGCATCATTAATAGTTCAAAATGGAACTCTAAATTCAGGTGATTCCATAATAGTTGGAACCACTTATGGA
AGAATTAGAGCTATGTTTGATGATAAGGGAAGAAAAATAAATTCAGCAGGACCATCAATTCCGGCAGAAATTTTAGGGCT
TTCAGAAGTACCTTCAGCGGGAGATAGATTCTATGTAGTAAAGGATGAAAAAACTGCAAGAGAAATGGCTGAGAAGAGAA
AAGAAAAAACTAGAAGTGAATACTTAGCAACTAGTAAAGTTTCATTAGAAGATTTATATAGTCAAATAAAAGAAGGAAAA
ATAAAAGAATTAAATATCATAGTTAAGGCAGATGTACAGGGAACTATTGAAGCTATAAGACAATCCCTTGAAAAATTATC
TACTGATGAAGTAAAAGTAAGAGTTATTCATGGTGGAGTTGGAGCTATTACGGAGACAGATGTAATTCTTGCTAATGCAT
CTAGTGCTGTAATTATAGGCTTTAATGTAAGACCAGATTCAAATGCTATAGTATCTGCTGAAAAAGAAAATGTAGAAATA
AAAACTTATAGAGTTATATACAGTGCCATTGAAGACATTAAAAAAGCTATGATAGGTATGCTAGAACCAGAATATAAAGA
AGTTATTCAAGGAAGAGCAGAAGTAAGAATGACATATAAAATATCTAATGTAGGAACAGTTGCAGGATGTTATGTTCAAA
GTGGTAAAATAACTAGAAATTCAGGTGTAAGAATTATAAGAGACGGTATAGTTATTTTCGAATCTGAATTATCATCTTTA
AAGAGATTCAAAGATGATGCTAAAGAAGTAGCCGCTGGATATGAATGTGGAATAATGGTTGAGAAATTTAATGATATAAA
AGAGGGCGACACAATAGAAGCTTATACAATGGAGACTATTAAAAAGCATACACTATGA

Upstream 100 bases:

>100_bases
GCCTTAGGAAAAGAAGGCTTAAATATAATAGGTATAAAAGATAAGTCTATGGCAAATAGATTGCTACAATTGTGGAATGG
TTCATAAATAAGTTTCGGGG

Downstream 100 bases:

>100_bases
TTGGAGTGATAAAATATGGCTAAGTATAGGGCTGGTAGAATAAATGAAGAAGTAAAAAAAGAAATTTCAAGCATAATCCG
AATGGATTTAAAAGATCCAA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MNALSKVRVYELSKELGISSKDLISLLKDEFSIEVKNHMSVVEEEDALLIKEFYGESKEENTEENIEEKYGDLQEAQIKV
VKKNRKNKVKKNDDENDAQEEIVIEMEDTITVKELSDRLKKTYAEVIKELMMMGVMAAVNQEIDFEAAEKLGEKFEAIVV
QREVDVLEESVEQYIEEEEEEGTVKRPPVVTVMGHVDHGKTSLLDAIRKEEVAASEAGGITQHIGAYTITINGEKITFLD
TPGHEAFTSMRARGAQITDIVILVVAADDGIMPQTEEAINHCKAANVPMVVAINKMDRAGANPDKVKQQLAEKGLVAEDW
GGDTITVPVSAHTKEGIDTLLEMVLLTAEMQELKSNPNRKAKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYG
RIRAMFDDKGRKINSAGPSIPAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKEKTRSEYLATSKVSLEDLYSQIKEGK
IKELNIIVKADVQGTIEAIRQSLEKLSTDEVKVRVIHGGVGAITETDVILANASSAVIIGFNVRPDSNAIVSAEKENVEI
KTYRVIYSAIEDIKKAMIGMLEPEYKEVIQGRAEVRMTYKISNVGTVAGCYVQSGKITRNSGVRIIRDGIVIFESELSSL
KRFKDDAKEVAAGYECGIMVEKFNDIKEGDTIEAYTMETIKKHTL

Sequences:

>Translated_685_residues
MNALSKVRVYELSKELGISSKDLISLLKDEFSIEVKNHMSVVEEEDALLIKEFYGESKEENTEENIEEKYGDLQEAQIKV
VKKNRKNKVKKNDDENDAQEEIVIEMEDTITVKELSDRLKKTYAEVIKELMMMGVMAAVNQEIDFEAAEKLGEKFEAIVV
QREVDVLEESVEQYIEEEEEEGTVKRPPVVTVMGHVDHGKTSLLDAIRKEEVAASEAGGITQHIGAYTITINGEKITFLD
TPGHEAFTSMRARGAQITDIVILVVAADDGIMPQTEEAINHCKAANVPMVVAINKMDRAGANPDKVKQQLAEKGLVAEDW
GGDTITVPVSAHTKEGIDTLLEMVLLTAEMQELKSNPNRKAKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYG
RIRAMFDDKGRKINSAGPSIPAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKEKTRSEYLATSKVSLEDLYSQIKEGK
IKELNIIVKADVQGTIEAIRQSLEKLSTDEVKVRVIHGGVGAITETDVILANASSAVIIGFNVRPDSNAIVSAEKENVEI
KTYRVIYSAIEDIKKAMIGMLEPEYKEVIQGRAEVRMTYKISNVGTVAGCYVQSGKITRNSGVRIIRDGIVIFESELSSL
KRFKDDAKEVAAGYECGIMVEKFNDIKEGDTIEAYTMETIKKHTL
>Mature_685_residues
MNALSKVRVYELSKELGISSKDLISLLKDEFSIEVKNHMSVVEEEDALLIKEFYGESKEENTEENIEEKYGDLQEAQIKV
VKKNRKNKVKKNDDENDAQEEIVIEMEDTITVKELSDRLKKTYAEVIKELMMMGVMAAVNQEIDFEAAEKLGEKFEAIVV
QREVDVLEESVEQYIEEEEEEGTVKRPPVVTVMGHVDHGKTSLLDAIRKEEVAASEAGGITQHIGAYTITINGEKITFLD
TPGHEAFTSMRARGAQITDIVILVVAADDGIMPQTEEAINHCKAANVPMVVAINKMDRAGANPDKVKQQLAEKGLVAEDW
GGDTITVPVSAHTKEGIDTLLEMVLLTAEMQELKSNPNRKAKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYG
RIRAMFDDKGRKINSAGPSIPAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKEKTRSEYLATSKVSLEDLYSQIKEGK
IKELNIIVKADVQGTIEAIRQSLEKLSTDEVKVRVIHGGVGAITETDVILANASSAVIIGFNVRPDSNAIVSAEKENVEI
KTYRVIYSAIEDIKKAMIGMLEPEYKEVIQGRAEVRMTYKISNVGTVAGCYVQSGKITRNSGVRIIRDGIVIFESELSSL
KRFKDDAKEVAAGYECGIMVEKFNDIKEGDTIEAYTMETIKKHTL

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=543, Percent_Identity=41.804788213628, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI53729337, Length=543, Percent_Identity=41.804788213628, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI84043963, Length=463, Percent_Identity=28.9416846652268, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI157426893, Length=247, Percent_Identity=24.6963562753036, Blast_Score=71, Evalue=4e-12,
Organism=Escherichia coli, GI1789559, Length=578, Percent_Identity=52.7681660899654, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI2367247, Length=251, Percent_Identity=32.6693227091633, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI48994988, Length=139, Percent_Identity=35.9712230215827, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788922, Length=238, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790412, Length=127, Percent_Identity=37.7952755905512, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789737, Length=127, Percent_Identity=37.7952755905512, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=540, Percent_Identity=34.0740740740741, Blast_Score=304, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI212656558, Length=566, Percent_Identity=26.5017667844523, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=27.1889400921659, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324550, Length=527, Percent_Identity=38.8994307400379, Blast_Score=352, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6319282, Length=514, Percent_Identity=27.431906614786, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28572034, Length=618, Percent_Identity=37.378640776699, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24656849, Length=447, Percent_Identity=30.2013422818792, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI160714833, Length=112, Percent_Identity=56.25, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI78706572, Length=247, Percent_Identity=28.7449392712551, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_CLOTE (Q895J8)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781905.1
- ProteinModelPortal:   Q895J8
- SMR:   Q895J8
- GeneID:   1059120
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01275
- NMPDR:   fig|212717.1.peg.1127
- HOGENOM:   HBG635083
- OMA:   GMRVTKG
- PhylomeDB:   Q895J8
- ProtClustDB:   PRK05306
- BioCyc:   CTET212717:CTC_01275-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 75658; Mature: 75658

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALSKVRVYELSKELGISSKDLISLLKDEFSIEVKNHMSVVEEEDALLIKEFYGESKEE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEHHHHHHHCCCCHHHHHHHCCCCCCC
NTEENIEEKYGDLQEAQIKVVKKNRKNKVKKNDDENDAQEEIVIEMEDTITVKELSDRLK
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEHHHHHHHHH
KTYAEVIKELMMMGVMAAVNQEIDFEAAEKLGEKFEAIVVQREVDVLEESVEQYIEEEEE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGTVKRPPVVTVMGHVDHGKTSLLDAIRKEEVAASEAGGITQHIGAYTITINGEKITFLD
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCEEEEEE
TPGHEAFTSMRARGAQITDIVILVVAADDGIMPQTEEAINHCKAANVPMVVAINKMDRAG
CCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCC
ANPDKVKQQLAEKGLVAEDWGGDTITVPVSAHTKEGIDTLLEMVLLTAEMQELKSNPNRK
CCHHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
AKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYGRIRAMFDDKGRKINSAGPSI
CCCCEEEEEECCCCCCHHHHEEECCCCCCCCEEEEECCHHHEEEEECCCCCEECCCCCCC
PAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKEKTRSEYLATSKVSLEDLYSQIKEGK
CHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
IKELNIIVKADVQGTIEAIRQSLEKLSTDEVKVRVIHGGVGAITETDVILANASSAVIIG
CEEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEECCEEEEECCCEEEEE
FNVRPDSNAIVSAEKENVEIKTYRVIYSAIEDIKKAMIGMLEPEYKEVIQGRAEVRMTYK
EEECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
ISNVGTVAGCYVQSGKITRNSGVRIIRDGIVIFESELSSLKRFKDDAKEVAAGYECGIMV
ECCCCCEEEEEEECCEEECCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEE
EKFNDIKEGDTIEAYTMETIKKHTL
ECCCCCCCCCCEEHHHHHHHHHHCC
>Mature Secondary Structure
MNALSKVRVYELSKELGISSKDLISLLKDEFSIEVKNHMSVVEEEDALLIKEFYGESKEE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEHHHHHHHCCCCHHHHHHHCCCCCCC
NTEENIEEKYGDLQEAQIKVVKKNRKNKVKKNDDENDAQEEIVIEMEDTITVKELSDRLK
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEHHHHHHHHH
KTYAEVIKELMMMGVMAAVNQEIDFEAAEKLGEKFEAIVVQREVDVLEESVEQYIEEEEE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGTVKRPPVVTVMGHVDHGKTSLLDAIRKEEVAASEAGGITQHIGAYTITINGEKITFLD
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCEEEEEE
TPGHEAFTSMRARGAQITDIVILVVAADDGIMPQTEEAINHCKAANVPMVVAINKMDRAG
CCCHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECHHCCC
ANPDKVKQQLAEKGLVAEDWGGDTITVPVSAHTKEGIDTLLEMVLLTAEMQELKSNPNRK
CCHHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
AKGTVVDAKLDKGRGPVASLIVQNGTLNSGDSIIVGTTYGRIRAMFDDKGRKINSAGPSI
CCCCEEEEEECCCCCCHHHHEEECCCCCCCCEEEEECCHHHEEEEECCCCCEECCCCCCC
PAEILGLSEVPSAGDRFYVVKDEKTAREMAEKRKEKTRSEYLATSKVSLEDLYSQIKEGK
CHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
IKELNIIVKADVQGTIEAIRQSLEKLSTDEVKVRVIHGGVGAITETDVILANASSAVIIG
CEEEEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEECCEEEEECCCEEEEE
FNVRPDSNAIVSAEKENVEIKTYRVIYSAIEDIKKAMIGMLEPEYKEVIQGRAEVRMTYK
EEECCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
ISNVGTVAGCYVQSGKITRNSGVRIIRDGIVIFESELSSLKRFKDDAKEVAAGYECGIMV
ECCCCCEEEEEEECCEEECCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEE
EKFNDIKEGDTIEAYTMETIKKHTL
ECCCCCCCCCCEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129