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The map label for this gene is nusA [H]

Identifier: 28210959

GI number: 28210959

Start: 1365613

End: 1366698

Strand: Direct

Name: nusA [H]

Synonym: CTC01273

Alternate gene names: 28210959

Gene position: 1365613-1366698 (Clockwise)

Preceding gene: 28210958

Following gene: 28210960

Centisome position: 48.78

GC content: 30.11

Gene sequence:

>1086_bases
ATGAATGAGGAATTCATAGTAGCATTAAAAGAAATTGTTAAAGAAAAGGGTATTAGTGCGGAACTACTTTTTAATACTAT
AGAAGATGCTTTAGTTGCTGCATATAAAAAGAATTACTCAAAACAAGGTGCAACAAGTCAACATGTAAATGTGTCTATGA
ATAGGGAAACTGGAGAAATCCATGTTTACTCAAGAAAAAATGTAGTAGAAGAAGTAGAAGATGAATTCAATGAAATATTC
GTAGAAGATGCTAAAACAATAGATCCTAGATATGAGTTAGAAGATATAGTTGATATAGAAGTTACACCAGAAAAATTTGG
AAGAGTAGCAGCTCAAGCGGCTAAACAAGTAGTTGTGCAAAGAATAAAGGAAGAAGAGAGAAGAATTGTATATAATGAAT
TCATAGAAAAAGAATGTGATATAATAACAGGTAATGTTATTAGAAAAGATAAAACCAATGTATTTATTAACTTAGGTAAA
ATAGAGGCAGTTCTAGGACCTAATGAACAGATGCCAGGAGAAGAATATAATTTTAATGATAAATTAAAACTATATATAGT
TGAAGTAAAAAATACTACTAAAGGTGCACAAGTAGTAGTATCTAGAACCCATCCAGGTCTAGTTAAGAGATTATTTGAAT
TAGAAGTGCCAGAAATATATAATGGTATTGTGGAAATAAAAAGTATTTCTAGAGAAGCAGGATCAAGAACAAAAATTGCA
GTACATTCAAATGATGAAAATGTTGACTCTATGGGAGCTTGTGTAGGTCCAAAAGGAACAAGAGTTCAAAATATAGTAAA
TGAACTTAAAAATGAAAAAATTGATATAATTAAATGGAGTAAGTTACCTGATGAATTTATATCAAATGCACTTAGTCCGG
CAAGAGTACTAAAAGTGGATGTAGATGAAGAAAGTAAATCAGCAAAAGTTTTAGTAGAAGATAATCAGCTTTCCCTAGCT
ATAGGTAAGGAAGGACAAAATGTTAGACTGGCTGCAAAATTAACAGGATGGAAAATAGATATAAAAAGCTTATCACAAAT
TTCAGAAGTAGAAGAGGATATAGAAGTTGTAGAAAGTGAAAATTAA

Upstream 100 bases:

>100_bases
AAACCTTACTTTAGAAATCAATGCTGAAGATTTTAAAATTCCACGTAAAAAGATTAAAAGAATAAATTTAATTTATGAAG
GGTAAAGGGAGGAAAATTAA

Downstream 100 bases:

>100_bases
ATAAAAGGAGGGTGATCATGTTGAAGACAAGGAAAATTCCTCAAAGGATGTGTACAGGTTGCATGGAAATGAAACCTAAA
AAAGAACTTATTAGAGTTGT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNEEFIVALKEIVKEKGISAELLFNTIEDALVAAYKKNYSKQGATSQHVNVSMNRETGEIHVYSRKNVVEEVEDEFNEIF
VEDAKTIDPRYELEDIVDIEVTPEKFGRVAAQAAKQVVVQRIKEEERRIVYNEFIEKECDIITGNVIRKDKTNVFINLGK
IEAVLGPNEQMPGEEYNFNDKLKLYIVEVKNTTKGAQVVVSRTHPGLVKRLFELEVPEIYNGIVEIKSISREAGSRTKIA
VHSNDENVDSMGACVGPKGTRVQNIVNELKNEKIDIIKWSKLPDEFISNALSPARVLKVDVDEESKSAKVLVEDNQLSLA
IGKEGQNVRLAAKLTGWKIDIKSLSQISEVEEDIEVVESEN

Sequences:

>Translated_361_residues
MNEEFIVALKEIVKEKGISAELLFNTIEDALVAAYKKNYSKQGATSQHVNVSMNRETGEIHVYSRKNVVEEVEDEFNEIF
VEDAKTIDPRYELEDIVDIEVTPEKFGRVAAQAAKQVVVQRIKEEERRIVYNEFIEKECDIITGNVIRKDKTNVFINLGK
IEAVLGPNEQMPGEEYNFNDKLKLYIVEVKNTTKGAQVVVSRTHPGLVKRLFELEVPEIYNGIVEIKSISREAGSRTKIA
VHSNDENVDSMGACVGPKGTRVQNIVNELKNEKIDIIKWSKLPDEFISNALSPARVLKVDVDEESKSAKVLVEDNQLSLA
IGKEGQNVRLAAKLTGWKIDIKSLSQISEVEEDIEVVESEN
>Mature_361_residues
MNEEFIVALKEIVKEKGISAELLFNTIEDALVAAYKKNYSKQGATSQHVNVSMNRETGEIHVYSRKNVVEEVEDEFNEIF
VEDAKTIDPRYELEDIVDIEVTPEKFGRVAAQAAKQVVVQRIKEEERRIVYNEFIEKECDIITGNVIRKDKTNVFINLGK
IEAVLGPNEQMPGEEYNFNDKLKLYIVEVKNTTKGAQVVVSRTHPGLVKRLFELEVPEIYNGIVEIKSISREAGSRTKIA
VHSNDENVDSMGACVGPKGTRVQNIVNELKNEKIDIIKWSKLPDEFISNALSPARVLKVDVDEESKSAKVLVEDNQLSLA
IGKEGQNVRLAAKLTGWKIDIKSLSQISEVEEDIEVVESEN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=348, Percent_Identity=37.6436781609195, Blast_Score=251, Evalue=5e-68,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 40675; Mature: 40675

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEFIVALKEIVKEKGISAELLFNTIEDALVAAYKKNYSKQGATSQHVNVSMNRETGEI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEE
HVYSRKNVVEEVEDEFNEIFVEDAKTIDPRYELEDIVDIEVTPEKFGRVAAQAAKQVVVQ
EEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
RIKEEERRIVYNEFIEKECDIITGNVIRKDKTNVFINLGKIEAVLGPNEQMPGEEYNFND
HHHHHHHHHHHHHHHHHCCCEEECCEEEECCCEEEEEECEEEEEECCCCCCCCCCCCCCC
KLKLYIVEVKNTTKGAQVVVSRTHPGLVKRLFELEVPEIYNGIVEIKSISREAGSRTKIA
EEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEE
VHSNDENVDSMGACVGPKGTRVQNIVNELKNEKIDIIKWSKLPDEFISNALSPARVLKVD
EECCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCEEEEEE
VDEESKSAKVLVEDNQLSLAIGKEGQNVRLAAKLTGWKIDIKSLSQISEVEEDIEVVESE
CCCCCCCCEEEEECCEEEEEECCCCCCEEEEEEECCEEEEHHHHHHHHHHHHHHHHHCCC
N
C
>Mature Secondary Structure
MNEEFIVALKEIVKEKGISAELLFNTIEDALVAAYKKNYSKQGATSQHVNVSMNRETGEI
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEE
HVYSRKNVVEEVEDEFNEIFVEDAKTIDPRYELEDIVDIEVTPEKFGRVAAQAAKQVVVQ
EEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
RIKEEERRIVYNEFIEKECDIITGNVIRKDKTNVFINLGKIEAVLGPNEQMPGEEYNFND
HHHHHHHHHHHHHHHHHCCCEEECCEEEECCCEEEEEECEEEEEECCCCCCCCCCCCCCC
KLKLYIVEVKNTTKGAQVVVSRTHPGLVKRLFELEVPEIYNGIVEIKSISREAGSRTKIA
EEEEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEE
VHSNDENVDSMGACVGPKGTRVQNIVNELKNEKIDIIKWSKLPDEFISNALSPARVLKVD
EECCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCEEEEEE
VDEESKSAKVLVEDNQLSLAIGKEGQNVRLAAKLTGWKIDIKSLSQISEVEEDIEVVESE
CCCCCCCCEEEEECCEEEEEECCCCCCEEEEEEECCEEEEHHHHHHHHHHHHHHHHHCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]