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The map label for this gene is trmD

Identifier: 28210937

GI number: 28210937

Start: 1341095

End: 1341814

Strand: Direct

Name: trmD

Synonym: CTC01251

Alternate gene names: 28210937

Gene position: 1341095-1341814 (Clockwise)

Preceding gene: 28210936

Following gene: 28210938

Centisome position: 47.91

GC content: 30.83

Gene sequence:

>720_bases
ATGGCTTTTAGAATAGACATATTAACCCTTTTTCCTGATATGTTTAATATATTTAACCACAGTATTATGGGGAAAGCTTT
AGAAAAGGATATAATTAATATAAATACCATAAATATAAGAGATTTTACAGAAGATAAACATAAAAAAGTAGATGACTACC
CCTATGGTGGCGGAGCAGGAATGGTAATGTCACTTCAACCTATAGTCGATTCCTTAAAGAGGGTAAAAGAAAGCAATAGG
GGAAAGGTTATATTTTTAGGACCAAGAGGAAAAACTTTTAGTCATGAAATGGCTAAGGAATTATCTAAAGAAGAAGAACT
GATATTTTTATGTGGACACTATGAAGGAATAGATGAAAGATGCTATGATTACATAGATTTAGAGATTTCTCTGGGAGATT
TTATCCTTACTGGAGGAGAAATGGCCTGTATACCTATAGTAGATAGTATATGCAGATTGATACCAGGTGTTTTGGGAAAA
AATGAAAGTTTTATGGAAGAATCTTTTTATGAAGGGCTTTTAGAATATCCTCATTATACTAGACCAGAGAATTTTAAGGG
AAAAGAGGTTCCTAAAATATTGTTATCTGGTCACCATGAAAATATCAGAAAATGGAGAAAGTCTAAATCCCTAAAAATAA
CAAAGAAAAGAAGACCAGATTTATTTAAAAAGTATAATCTTACAAAAGAAGATGAGAAACTTCTATCTAGTGAAGAATAA

Upstream 100 bases:

>100_bases
GGGTAGAAGGAAAAAAAGAATTATTAATTCCTGTTCTAAAGACTATTGTGGTTAAAATAGATATAGAAAATGAAGAAATA
ATAATAAAACCTGTAAAAAC

Downstream 100 bases:

>100_bases
AAGTTTATGTTGAAAAATACATATACATATGATAAAATTATTTTCGTGCTAGTACGGGCGGTCCTCTGTTTATTGTAAGT
AAATAAAGTATGAACGTCAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAFRIDILTLFPDMFNIFNHSIMGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNR
GKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGK
NESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRPDLFKKYNLTKEDEKLLSSEE

Sequences:

>Translated_239_residues
MAFRIDILTLFPDMFNIFNHSIMGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNR
GKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGK
NESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRPDLFKKYNLTKEDEKLLSSEE
>Mature_238_residues
AFRIDILTLFPDMFNIFNHSIMGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAGMVMSLQPIVDSLKRVKESNRG
KVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDERCYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKN
ESFMEESFYEGLLEYPHYTRPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRPDLFKKYNLTKEDEKLLSSEE

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=236, Percent_Identity=46.1864406779661, Blast_Score=224, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CLOTE (Q895M2)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781881.1
- ProteinModelPortal:   Q895M2
- SMR:   Q895M2
- GeneID:   1059137
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01251
- NMPDR:   fig|212717.1.peg.1103
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   Q895M2
- ProtClustDB:   PRK00026
- BioCyc:   CTET212717:CTC_01251-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27648; Mature: 27517

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRIDILTLFPDMFNIFNHSIMGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAG
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHCCCCCCCCCCCCC
MVMSLQPIVDSLKRVKESNRGKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDER
CEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHH
CYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKNESFMEESFYEGLLEYPHYT
HHEEEEEEEECCCEEEECCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
RPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRPDLFKKYNLTKEDEKLLSSEE
CCCCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEEHHCCCHHHHHCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AFRIDILTLFPDMFNIFNHSIMGKALEKDIININTINIRDFTEDKHKKVDDYPYGGGAG
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHCCCCCCCCCCCCC
MVMSLQPIVDSLKRVKESNRGKVIFLGPRGKTFSHEMAKELSKEEELIFLCGHYEGIDER
CEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHH
CYDYIDLEISLGDFILTGGEMACIPIVDSICRLIPGVLGKNESFMEESFYEGLLEYPHYT
HHEEEEEEEECCCEEEECCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
RPENFKGKEVPKILLSGHHENIRKWRKSKSLKITKKRRPDLFKKYNLTKEDEKLLSSEE
CCCCCCCCHHHHHHHCCCHHHHHHHHHCCCEEEEHHCCCHHHHHCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129