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The map label for this gene is ackA

Identifier: 28210927

GI number: 28210927

Start: 1327414

End: 1328616

Strand: Direct

Name: ackA

Synonym: CTC01240

Alternate gene names: 28210927

Gene position: 1327414-1328616 (Clockwise)

Preceding gene: 28210926

Following gene: 28210928

Centisome position: 47.42

GC content: 31.17

Gene sequence:

>1203_bases
TTGAAAATTTTAGTAATAAATTGTGGCAGTTCATCTCTTAAATATCAATTTATAAACATGACCAACGAAGACGTTTTAGC
TAAAGGACTTGTAGAAAGAATAGGGATGGAAGGTTCTATTTTAACTCATAAAGTTAATGATGAAAAATATGTAATACAAG
AACCTATGAAGGATCATAAAGATGCTATAAAATTGGTTTTAGACGCTTTAACTGATAAGGAACATGGTGTTATAGAGGAT
ATGTCCGAAATATCCTCAGTTGGACACAGAGTGGTTCATGGAGGAGAAAAATATGCAAATTCTGTATTAATAGACGAAGA
TGTAATGAAATCTTTAGAAGAATGCATAAAACTTGCTCCATTACACAATCCACCAAATATTATAGGTATAAATGCATGTA
AAGCTTTAATGCCAAATACTCCAATGGTTGCAGTATTTGATACAGCTTTCCATCAAACTATACCAGAATATGCTTATATA
TATCCATTACCATATGAACTATATAAAAAATATGGAATAAGAAAATATGGATTCCACGGAACATCACATAGATATGTATC
TAAAAGAGTAGCAGAGTTGATGGGTAAAGACATAAAAGACTTAAAAATAATAACTTGTCATCTTGGAAATGGTGCAAGTA
TTTGTGCTGTTAATGAAGGAAAATCCGTTGAAACTAGCATGGGCTTTACACCACTTGAAGGAATAGCTATGGGAACAAGA
TCAGGAAGTATAGATCCAGCGATATTACCATTTTTAATGAAAGAATTAAACTTAAATTTAGATGATTTAAATAATCTTTT
AAATAAAAAATCTGGTGTATATGGAATATCTGGAGTTAGTAGTGACTTTAGAGATATAGAAGAAGCGGCTGAAGAAGGAA
ATGATAGAGCTAAACTTGCATTAGATGTTTTCCATTACAAGGTTAGAAGATTTATAGGTTCTTATGCTGCAGCTATGGGT
GGAGTAGATTGTGTTGTATTTACAGCAGGACTTGGAGAAAACTCTGCAGAAAGTAGAGAAGAAATATGCAAAAACTTAGA
ATTCTTAGGAATAAAAATAAATGAAAAGAAGAATAATGAAGGAAGAGGATTTGCCAAAGTTATAAGTGAAGACAACTCAA
AAGTAAAGGTTTTTGTAATACCAACAGACGAAGAACTTATGATAGCAAGAGATACTAATGAAATTGTTAGTAATCTTAAA
TAG

Upstream 100 bases:

>100_bases
AGTAAACGTAGTAGCTGTTACTGCAGTGCAAGCTCAAAACCAATAAAAGGTAGTTAACCATTAAAATATATTAAGGTTCA
AACTGAAAGGAGATTTTAAA

Downstream 100 bases:

>100_bases
GAAAATTTAAATAAATCTTGACTTTTAATTCCTGTCTTTATATAATAAATTGTGGCTAAGCATTGAGGTGAGTTTTATGA
AAATAGATGTATCAAAGCTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKILVINCGSSSLKYQFINMTNEDVLAKGLVERIGMEGSILTHKVNDEKYVIQEPMKDHKDAIKLVLDALTDKEHGVIED
MSEISSVGHRVVHGGEKYANSVLIDEDVMKSLEECIKLAPLHNPPNIIGINACKALMPNTPMVAVFDTAFHQTIPEYAYI
YPLPYELYKKYGIRKYGFHGTSHRYVSKRVAELMGKDIKDLKIITCHLGNGASICAVNEGKSVETSMGFTPLEGIAMGTR
SGSIDPAILPFLMKELNLNLDDLNNLLNKKSGVYGISGVSSDFRDIEEAAEEGNDRAKLALDVFHYKVRRFIGSYAAAMG
GVDCVVFTAGLGENSAESREEICKNLEFLGIKINEKKNNEGRGFAKVISEDNSKVKVFVIPTDEELMIARDTNEIVSNLK

Sequences:

>Translated_400_residues
MKILVINCGSSSLKYQFINMTNEDVLAKGLVERIGMEGSILTHKVNDEKYVIQEPMKDHKDAIKLVLDALTDKEHGVIED
MSEISSVGHRVVHGGEKYANSVLIDEDVMKSLEECIKLAPLHNPPNIIGINACKALMPNTPMVAVFDTAFHQTIPEYAYI
YPLPYELYKKYGIRKYGFHGTSHRYVSKRVAELMGKDIKDLKIITCHLGNGASICAVNEGKSVETSMGFTPLEGIAMGTR
SGSIDPAILPFLMKELNLNLDDLNNLLNKKSGVYGISGVSSDFRDIEEAAEEGNDRAKLALDVFHYKVRRFIGSYAAAMG
GVDCVVFTAGLGENSAESREEICKNLEFLGIKINEKKNNEGRGFAKVISEDNSKVKVFVIPTDEELMIARDTNEIVSNLK
>Mature_400_residues
MKILVINCGSSSLKYQFINMTNEDVLAKGLVERIGMEGSILTHKVNDEKYVIQEPMKDHKDAIKLVLDALTDKEHGVIED
MSEISSVGHRVVHGGEKYANSVLIDEDVMKSLEECIKLAPLHNPPNIIGINACKALMPNTPMVAVFDTAFHQTIPEYAYI
YPLPYELYKKYGIRKYGFHGTSHRYVSKRVAELMGKDIKDLKIITCHLGNGASICAVNEGKSVETSMGFTPLEGIAMGTR
SGSIDPAILPFLMKELNLNLDDLNNLLNKKSGVYGISGVSSDFRDIEEAAEEGNDRAKLALDVFHYKVRRFIGSYAAAMG
GVDCVVFTAGLGENSAESREEICKNLEFLGIKINEKKNNEGRGFAKVISEDNSKVKVFVIPTDEELMIARDTNEIVSNLK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=403, Percent_Identity=43.424317617866, Blast_Score=331, Evalue=4e-92,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=41.7302798982188, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_CLOTE (Q895N2)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781871.1
- ProteinModelPortal:   Q895N2
- SMR:   Q895N2
- GeneID:   1059142
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01240
- NMPDR:   fig|212717.1.peg.1093
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- PhylomeDB:   Q895N2
- ProtClustDB:   PRK00180
- BioCyc:   CTET212717:CTC_01240-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 44230; Mature: 44230

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVINCGSSSLKYQFINMTNEDVLAKGLVERIGMEGSILTHKVNDEKYVIQEPMKDHK
CEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHCHHHHHH
DAIKLVLDALTDKEHGVIEDMSEISSVGHRVVHGGEKYANSVLIDEDVMKSLEECIKLAP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACKALMPNTPMVAVFDTAFHQTIPEYAYIYPLPYELYKKYGIRKYGFHG
CCCCCCEEEECHHHHHCCCCCEEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCCC
TSHRYVSKRVAELMGKDIKDLKIITCHLGNGASICAVNEGKSVETSMGFTPLEGIAMGTR
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEECCC
SGSIDPAILPFLMKELNLNLDDLNNLLNKKSGVYGISGVSSDFRDIEEAAEEGNDRAKLA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCHHHHH
LDVFHYKVRRFIGSYAAAMGGVDCVVFTAGLGENSAESREEICKNLEFLGIKINEKKNNE
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCC
GRGFAKVISEDNSKVKVFVIPTDEELMIARDTNEIVSNLK
CCHHHHHHCCCCCEEEEEEEECCCCEEEEECHHHHHHCCC
>Mature Secondary Structure
MKILVINCGSSSLKYQFINMTNEDVLAKGLVERIGMEGSILTHKVNDEKYVIQEPMKDHK
CEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEHHCHHHHHH
DAIKLVLDALTDKEHGVIEDMSEISSVGHRVVHGGEKYANSVLIDEDVMKSLEECIKLAP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACKALMPNTPMVAVFDTAFHQTIPEYAYIYPLPYELYKKYGIRKYGFHG
CCCCCCEEEECHHHHHCCCCCEEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCCC
TSHRYVSKRVAELMGKDIKDLKIITCHLGNGASICAVNEGKSVETSMGFTPLEGIAMGTR
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEECCC
SGSIDPAILPFLMKELNLNLDDLNNLLNKKSGVYGISGVSSDFRDIEEAAEEGNDRAKLA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCHHHHH
LDVFHYKVRRFIGSYAAAMGGVDCVVFTAGLGENSAESREEICKNLEFLGIKINEKKNNE
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCC
GRGFAKVISEDNSKVKVFVIPTDEELMIARDTNEIVSNLK
CCHHHHHHCCCCCEEEEEEEECCCCEEEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129