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The map label for this gene is cca [H]

Identifier: 28210893

GI number: 28210893

Start: 1289376

End: 1290731

Strand: Direct

Name: cca [H]

Synonym: CTC01205

Alternate gene names: 28210893

Gene position: 1289376-1290731 (Clockwise)

Preceding gene: 28210892

Following gene: 28210894

Centisome position: 46.06

GC content: 25.74

Gene sequence:

>1356_bases
ATGGTGGAGAAAAATATAAAAATAAACATGCCAAAGGAAGTAAAATATATTATAGATATTTTAGAAGAACATGGATATGA
AGCTTATGCCGTAGGAGGATGTATTAGGGATAGCCTTTTAAATAGAATTCCACATGACTGGGATATTACAACAGATGCCA
AAGGAGAGCAGGTTATAAAAATATTTAAATCATTGGATTTCAATGTAGTGAAAACTGGAATTAGATATGGAACAGTAACC
CTTATTATAAATTCTATTGGATACGAAATAACAACCTATAGAATTGATGGTAATTACTTAGATGGTAGACATCCAGAAAA
GGTAGAATTTACTAATAATTTAAAAGAAGATTTAAGCCGTAGAGATTTTACAGTAAATGCTATGGCATATAATCACAAGA
AAGGATTAGCTGATTATTATAACGGAAGAGAGGATTTAAAAAATAAAATTATTAAATCTGTTGGGGATCCTTTAAAAAGA
TTTTCAGAAGATGCCCTAAGAATGATTAGAGGAGTTAGATTTTCTGCCCAATTGGATTTTAACATAGAAGAAAATACAAA
AGAGGCTATAAAAAAATTAAGTAGTAATATTAAAAATGTATCTGTAGAAAGAATCAGAGAAGAATTTAATAAAATTTTAT
TGTCTTCCAATACATATAAAATTAAAGAATTAGAGGAATATGGTCTTTTAAAAAATTTTTTACCAGAATATGCTTTTACT
AAGAGAATAGTACAAAATAATCAATACCATATATACAATTTAGAGGAACATTTAATTTGTAGTATAGAAAATGTAGATAA
AAAATTACATTTAAGACTTACTATGCTTTTACATGATATAGGAAAATCTCAATGTAAGACTACAGATAATAAAGGTGTAG
ATCATTTTTGTGGTCATGAAAAAATATCTAGTGAAATGGCAGGAAGCATTTTAAGAAGGTTAAAATACGATAATAAAACC
ATAGATAAAGTTACTTCTTTAATAAAATATCATCATTATCCAATAGAGAATAAAAGAAATATTAAAAAATTATTAAATAA
AATAGGATTAGAACTTTTTAGGGATTTATTAAAAGTTAAAGAAGGGGACATAAAAGCCCAAAACCCTATGTTTTTTAAAA
AGAGATATGAAAGTATATTAGAAGCAAAAAAAGTTTTAGAAGAAATATTAAAAGACAAAGAATGTTTTACTTTAAAGGAT
TTAGCTATTAATGGGAAGGATTTAATAGACTTAGGATATAAGGATGGAAGGAACATAGGTAAAACTTTAAACACATTATT
AGAATTGGTTTTAGAGAAGCCAGAACTAAATAAAAAAGAAGAATTAATAAAATATATTAAAAAAGAGGAATCCTAA

Upstream 100 bases:

>100_bases
TTAATATATGAGAAATATTTCCAATGAAATATAAAAAATAATAAAAATAGAATGGTATATTATTATGTAGAATTAACATT
GAAACCGAAGGAGTTTTAAT

Downstream 100 bases:

>100_bases
GCATCATAATGTAGGATTCTTTTTTTCTTTTTTGGATCCACTTTGAATAAGCTTTAGCTTGTCACATCGTTTAAATTGTT
TTATTTCATATTCCCGTTTT

Product: poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MVEKNIKINMPKEVKYIIDILEEHGYEAYAVGGCIRDSLLNRIPHDWDITTDAKGEQVIKIFKSLDFNVVKTGIRYGTVT
LIINSIGYEITTYRIDGNYLDGRHPEKVEFTNNLKEDLSRRDFTVNAMAYNHKKGLADYYNGREDLKNKIIKSVGDPLKR
FSEDALRMIRGVRFSAQLDFNIEENTKEAIKKLSSNIKNVSVERIREEFNKILLSSNTYKIKELEEYGLLKNFLPEYAFT
KRIVQNNQYHIYNLEEHLICSIENVDKKLHLRLTMLLHDIGKSQCKTTDNKGVDHFCGHEKISSEMAGSILRRLKYDNKT
IDKVTSLIKYHHYPIENKRNIKKLLNKIGLELFRDLLKVKEGDIKAQNPMFFKKRYESILEAKKVLEEILKDKECFTLKD
LAINGKDLIDLGYKDGRNIGKTLNTLLELVLEKPELNKKEELIKYIKKEES

Sequences:

>Translated_451_residues
MVEKNIKINMPKEVKYIIDILEEHGYEAYAVGGCIRDSLLNRIPHDWDITTDAKGEQVIKIFKSLDFNVVKTGIRYGTVT
LIINSIGYEITTYRIDGNYLDGRHPEKVEFTNNLKEDLSRRDFTVNAMAYNHKKGLADYYNGREDLKNKIIKSVGDPLKR
FSEDALRMIRGVRFSAQLDFNIEENTKEAIKKLSSNIKNVSVERIREEFNKILLSSNTYKIKELEEYGLLKNFLPEYAFT
KRIVQNNQYHIYNLEEHLICSIENVDKKLHLRLTMLLHDIGKSQCKTTDNKGVDHFCGHEKISSEMAGSILRRLKYDNKT
IDKVTSLIKYHHYPIENKRNIKKLLNKIGLELFRDLLKVKEGDIKAQNPMFFKKRYESILEAKKVLEEILKDKECFTLKD
LAINGKDLIDLGYKDGRNIGKTLNTLLELVLEKPELNKKEELIKYIKKEES
>Mature_451_residues
MVEKNIKINMPKEVKYIIDILEEHGYEAYAVGGCIRDSLLNRIPHDWDITTDAKGEQVIKIFKSLDFNVVKTGIRYGTVT
LIINSIGYEITTYRIDGNYLDGRHPEKVEFTNNLKEDLSRRDFTVNAMAYNHKKGLADYYNGREDLKNKIIKSVGDPLKR
FSEDALRMIRGVRFSAQLDFNIEENTKEAIKKLSSNIKNVSVERIREEFNKILLSSNTYKIKELEEYGLLKNFLPEYAFT
KRIVQNNQYHIYNLEEHLICSIENVDKKLHLRLTMLLHDIGKSQCKTTDNKGVDHFCGHEKISSEMAGSILRRLKYDNKT
IDKVTSLIKYHHYPIENKRNIKKLLNKIGLELFRDLLKVKEGDIKAQNPMFFKKRYESILEAKKVLEEILKDKECFTLKD
LAINGKDLIDLGYKDGRNIGKTLNTLLELVLEKPELNKKEELIKYIKKEES

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=209, Percent_Identity=35.8851674641148, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI87081691, Length=262, Percent_Identity=32.0610687022901, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789436, Length=346, Percent_Identity=24.8554913294798, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71995920, Length=201, Percent_Identity=32.8358208955224, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321016, Length=289, Percent_Identity=26.2975778546713, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281360112, Length=206, Percent_Identity=33.0097087378641, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357337, Length=206, Percent_Identity=33.0097087378641, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52607; Mature: 52607

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKNIKINMPKEVKYIIDILEEHGYEAYAVGGCIRDSLLNRIPHDWDITTDAKGEQVIK
CCCCCEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
IFKSLDFNVVKTGIRYGTVTLIINSIGYEITTYRIDGNYLDGRHPEKVEFTNNLKEDLSR
HHHHCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
RDFTVNAMAYNHKKGLADYYNGREDLKNKIIKSVGDPLKRFSEDALRMIRGVRFSAQLDF
CCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEC
NIEENTKEAIKKLSSNIKNVSVERIREEFNKILLSSNTYKIKELEEYGLLKNFLPEYAFT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
KRIVQNNQYHIYNLEEHLICSIENVDKKLHLRLTMLLHDIGKSQCKTTDNKGVDHFCGHE
HHHHHCCCEEEEECHHHHEEEHHHCCHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHCCHH
KISSEMAGSILRRLKYDNKTIDKVTSLIKYHHYPIENKRNIKKLLNKIGLELFRDLLKVK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
EGDIKAQNPMFFKKRYESILEAKKVLEEILKDKECFTLKDLAINGKDLIDLGYKDGRNIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCHHEECCCCCCCHHH
KTLNTLLELVLEKPELNKKEELIKYIKKEES
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVEKNIKINMPKEVKYIIDILEEHGYEAYAVGGCIRDSLLNRIPHDWDITTDAKGEQVIK
CCCCCEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
IFKSLDFNVVKTGIRYGTVTLIINSIGYEITTYRIDGNYLDGRHPEKVEFTNNLKEDLSR
HHHHCCHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
RDFTVNAMAYNHKKGLADYYNGREDLKNKIIKSVGDPLKRFSEDALRMIRGVRFSAQLDF
CCEEEEEEEECCCCCCHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEC
NIEENTKEAIKKLSSNIKNVSVERIREEFNKILLSSNTYKIKELEEYGLLKNFLPEYAFT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHH
KRIVQNNQYHIYNLEEHLICSIENVDKKLHLRLTMLLHDIGKSQCKTTDNKGVDHFCGHE
HHHHHCCCEEEEECHHHHEEEHHHCCHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHCCHH
KISSEMAGSILRRLKYDNKTIDKVTSLIKYHHYPIENKRNIKKLLNKIGLELFRDLLKVK
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
EGDIKAQNPMFFKKRYESILEAKKVLEEILKDKECFTLKDLAINGKDLIDLGYKDGRNIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCHHEECCCCCCCHHH
KTLNTLLELVLEKPELNKKEELIKYIKKEES
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA