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The map label for this gene is purC

Identifier: 28210862

GI number: 28210862

Start: 1249155

End: 1249838

Strand: Direct

Name: purC

Synonym: CTC01170

Alternate gene names: 28210862

Gene position: 1249155-1249838 (Clockwise)

Preceding gene: 28210860

Following gene: 28210863

Centisome position: 44.62

GC content: 30.99

Gene sequence:

>684_bases
ATGAACGTTGTTTATAAAGGTAAAACAAAGGATGTATTTCTTTTAGAAGATGGTAATTACTTACTTAAATTTAAAGATGA
TGTAACAGGAGAAAATGGAGTTTTCGATCCAGGTGCAAATAGTGTTGGATTAACTATGGAAGGAGCGGGTAGGGCAGCTC
TTAGGTTAACTAAATTATTTTTTGAAAGATTAAAAGAAGAAAAAATACCAACCCATTATATAGATTCAAATGTAGAAGAA
GGCACAATGAAGGTAAAACCTGCTAAAATATTTGGAAATGGACTTGAAGTAATTTGTAGATATAGAGCAGTTGGAAGTTT
TATGCGTCGTTATGGTATGTACGCAAAAGAAGGACAAGTTTTGGATGCTTTTGTGGAAGTTACTCTTAAGGATGACGAAC
GTCAAGATCCACCAATTACTAAGGATGCACTTGATATGCTTGGAATACTTTCTTTGAGAGAATATGATATATTAAAAGAC
CTTACTAAAAAAATAAGCGGTATAGTGAAGGATGAGTTATCTAAAAAAGGTATAGAACTATATGATATTAAACTTGAATT
TGGAAGAATAGACGAAGATAATCATATTGCATTAATAGATGAAATTTCTGGTGGAAATATGAGAGCATATAAGGATGGAG
AATATATAGAACCTTTGGATTTAGAAAAATTAATCATTGAATAA

Upstream 100 bases:

>100_bases
GAATATTAAGATTGATTTTTATAAAAAAGTTAGTATATAATAAATGTATGGCCAATATAGGAAATAAGAATATAAAATTT
TAATTTATAGGAGTGGTTTC

Downstream 100 bases:

>100_bases
TAGTGTTTTAAAAGCTAACTTTAATATGAATTAATTACAATTAAGTAGATATTATTAAAAAACTTGTTATTTTCTAAGCC
GTACACTTAAATTTAATTAG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNVVYKGKTKDVFLLEDGNYLLKFKDDVTGENGVFDPGANSVGLTMEGAGRAALRLTKLFFERLKEEKIPTHYIDSNVEE
GTMKVKPAKIFGNGLEVICRYRAVGSFMRRYGMYAKEGQVLDAFVEVTLKDDERQDPPITKDALDMLGILSLREYDILKD
LTKKISGIVKDELSKKGIELYDIKLEFGRIDEDNHIALIDEISGGNMRAYKDGEYIEPLDLEKLIIE

Sequences:

>Translated_227_residues
MNVVYKGKTKDVFLLEDGNYLLKFKDDVTGENGVFDPGANSVGLTMEGAGRAALRLTKLFFERLKEEKIPTHYIDSNVEE
GTMKVKPAKIFGNGLEVICRYRAVGSFMRRYGMYAKEGQVLDAFVEVTLKDDERQDPPITKDALDMLGILSLREYDILKD
LTKKISGIVKDELSKKGIELYDIKLEFGRIDEDNHIALIDEISGGNMRAYKDGEYIEPLDLEKLIIE
>Mature_227_residues
MNVVYKGKTKDVFLLEDGNYLLKFKDDVTGENGVFDPGANSVGLTMEGAGRAALRLTKLFFERLKEEKIPTHYIDSNVEE
GTMKVKPAKIFGNGLEVICRYRAVGSFMRRYGMYAKEGQVLDAFVEVTLKDDERQDPPITKDALDMLGILSLREYDILKD
LTKKISGIVKDELSKKGIELYDIKLEFGRIDEDNHIALIDEISGGNMRAYKDGEYIEPLDLEKLIIE

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=220, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5453539, Length=220, Percent_Identity=29.0909090909091, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788820, Length=225, Percent_Identity=26.6666666666667, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_CLOTE (Q895U7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781806.1
- ProteinModelPortal:   Q895U7
- SMR:   Q895U7
- GeneID:   1059290
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC01170
- NMPDR:   fig|212717.1.peg.1028
- HOGENOM:   HBG306070
- OMA:   YLLKFKD
- PhylomeDB:   Q895U7
- ProtClustDB:   CLSK912113
- BioCyc:   CTET212717:CTC_01170-MONOMER
- BRENDA:   6.3.2.6
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 25699; Mature: 25699

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVYKGKTKDVFLLEDGNYLLKFKDDVTGENGVFDPGANSVGLTMEGAGRAALRLTKLF
CCEEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
FERLKEEKIPTHYIDSNVEEGTMKVKPAKIFGNGLEVICRYRAVGSFMRRYGMYAKEGQV
HHHHHHHCCCCEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCE
LDAFVEVTLKDDERQDPPITKDALDMLGILSLREYDILKDLTKKISGIVKDELSKKGIEL
EEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
YDIKLEFGRIDEDNHIALIDEISGGNMRAYKDGEYIEPLDLEKLIIE
EEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MNVVYKGKTKDVFLLEDGNYLLKFKDDVTGENGVFDPGANSVGLTMEGAGRAALRLTKLF
CCEEECCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
FERLKEEKIPTHYIDSNVEEGTMKVKPAKIFGNGLEVICRYRAVGSFMRRYGMYAKEGQV
HHHHHHHCCCCEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCE
LDAFVEVTLKDDERQDPPITKDALDMLGILSLREYDILKDLTKKISGIVKDELSKKGIEL
EEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
YDIKLEFGRIDEDNHIALIDEISGGNMRAYKDGEYIEPLDLEKLIIE
EEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129