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The map label for this gene is sigK [H]

Identifier: 28210768

GI number: 28210768

Start: 1137516

End: 1138064

Strand: Direct

Name: sigK [H]

Synonym: CTC01066

Alternate gene names: 28210768

Gene position: 1137516-1138064 (Clockwise)

Preceding gene: 28210767

Following gene: 28210773

Centisome position: 40.64

GC content: 28.42

Gene sequence:

>549_bases
TTGATACTTAAAGAAAATTTATATAAAATAAGAACTAAAATTAGTCTCTCTGAATATTATAATAATAAGCACTATTTGGA
GGGACTAAAAGTGTATATGTTTGATTCAATAATAAACATATTAGGTAGCAATATACTATTAACAGCCTATGTTACAGGTA
ATAATTCTTTTCCAAAACCATTAAAGGCAGAAGAAGAAAAATATTATTTAAAACAATTGGAAAAAGGGGATATATCAGCA
AAAGGAGTACTTATAGAAAGAAATTTAAGACTTGTAGCCCACATAGTAAAAAAATATTCATTTCCCGGAAAGGATATGGA
TGATTTAATATCTATAGGAACTATAGGTCTTATAAAAGCCATAGATTCATTTAAAATAGACAGAGGCACAAGACTCGCTA
CTTATGCAGCAAAGTGCATTGAAAATGAAATATTAATGCTTATTAGAAATAACAAAAAAACAAAAGGAGAGGTATATCTT
CAAGATCCAATAGGAATAGATAAAGAAGGCAATGAAATACCCTGGACAAGTTATCAGTCTAGGGCTTAA

Upstream 100 bases:

>100_bases
AGATTCTGAAAGTGGTGGAAATACTGCAGCACCTATCTTTAAAGATGTAGTTAAGATATTTCTAGATGAAGAACATTAAT
ACAAGTAAAAGTTATTTTGT

Downstream 100 bases:

>100_bases
GTTTAGGAAATATATCTATTCCAAAGGTATCTCCACGTTTATTCTTAGTATACTCAACCTTATTTACTAACTTTTTCATC
AACTCATTTTTCAATTTAAT

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MILKENLYKIRTKISLSEYYNNKHYLEGLKVYMFDSIINILGSNILLTAYVTGNNSFPKPLKAEEEKYYLKQLEKGDISA
KGVLIERNLRLVAHIVKKYSFPGKDMDDLISIGTIGLIKAIDSFKIDRGTRLATYAAKCIENEILMLIRNNKKTKGEVYL
QDPIGIDKEGNEIPWTSYQSRA

Sequences:

>Translated_182_residues
MILKENLYKIRTKISLSEYYNNKHYLEGLKVYMFDSIINILGSNILLTAYVTGNNSFPKPLKAEEEKYYLKQLEKGDISA
KGVLIERNLRLVAHIVKKYSFPGKDMDDLISIGTIGLIKAIDSFKIDRGTRLATYAAKCIENEILMLIRNNKKTKGEVYL
QDPIGIDKEGNEIPWTSYQSRA
>Mature_182_residues
MILKENLYKIRTKISLSEYYNNKHYLEGLKVYMFDSIINILGSNILLTAYVTGNNSFPKPLKAEEEKYYLKQLEKGDISA
KGVLIERNLRLVAHIVKKYSFPGKDMDDLISIGTIGLIKAIDSFKIDRGTRLATYAAKCIENEILMLIRNNKKTKGEVYL
QDPIGIDKEGNEIPWTSYQSRA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=124, Percent_Identity=37.0967741935484, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1789871, Length=99, Percent_Identity=38.3838383838384, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 20831; Mature: 20831

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKENLYKIRTKISLSEYYNNKHYLEGLKVYMFDSIINILGSNILLTAYVTGNNSFPKP
CCCHHHHHHHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
LKAEEEKYYLKQLEKGDISAKGVLIERNLRLVAHIVKKYSFPGKDMDDLISIGTIGLIKA
CCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IDSFKIDRGTRLATYAAKCIENEILMLIRNNKKTKGEVYLQDPIGIDKEGNEIPWTSYQS
HHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RA
CC
>Mature Secondary Structure
MILKENLYKIRTKISLSEYYNNKHYLEGLKVYMFDSIINILGSNILLTAYVTGNNSFPKP
CCCHHHHHHHHHHEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
LKAEEEKYYLKQLEKGDISAKGVLIERNLRLVAHIVKKYSFPGKDMDDLISIGTIGLIKA
CCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IDSFKIDRGTRLATYAAKCIENEILMLIRNNKKTKGEVYLQDPIGIDKEGNEIPWTSYQS
HHHHCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]