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The map label for this gene is yqiI [H]

Identifier: 28210714

GI number: 28210714

Start: 1078067

End: 1079860

Strand: Direct

Name: yqiI [H]

Synonym: CTC01009

Alternate gene names: 28210714

Gene position: 1078067-1079860 (Clockwise)

Preceding gene: 28210713

Following gene: 28210715

Centisome position: 38.51

GC content: 31.55

Gene sequence:

>1794_bases
TTGATCATGAAAAATAAAAAGAAATGGTGGTTTTTATTTGTAGCAGTAGCAGTTTTTACGTTTTTAGGAAGCTTTAATTT
TGTTAAAGCAGAAGAAGTTTATGATGACAAAGGTGTAGAGTTAAATGTACATTCGAGAAAGTCTTGGACTGTAAAGTTTA
ACAATACACTAGATCCTGCCACTATAACAGGAGATAATATAGTGGTTTTAAACGAAAATGGTGGAAAAGTTCCTGTGAAA
TTAGAGATAGGATCTAAGGGTAAAACGGTAGTAGTCACGCCTTCAAAATCATATGATTTTGAAAAAAAATATAAAATGAT
CTTAAAGGATGGTGTAAAATCTAAATCAGGAAAGAAATTAATAAAATCTGCAAAATTAGATTTTACAATTAGAAATAAGG
ATGATGAATATAAAAGCTATACCGTTTGTATTGATGCAGGGCATGGAGGCGAAGATAGTGGTTATGTATCTCCTTATGGA
CTTAAAGAAAAATCTATAAACTTATCTGTGGCGTTAGAAGTTGGAAAGATGTTAGAGGAAAAAGGGATTAATGTAATTTA
CACTAGAAAAGATGATAATATCCCTTGGAGTAGTGATGAAGATCTTAACTATCGATTTAATATGGCAAACAATAATAAGG
CTGATTATTTTGTGAGTATACATTGTAATTATAGTGATAACCCTATTGACAGCGGTATAGAAACATATTATAGAGATTGG
GATTCTATTTCACAAAATTTAAGTAAAGCAGTACAAGATGAAGTAATAAACAATACCCAAGGAAGAAATAGAGGAATAAA
ACCAGCACTTCCAGAACATGAAATATTACTTGGTACGCAGGCTCATGCAATAATGGTGAATCTAGGATTTATGAGTAATG
CTGAGGAAACTAATATACTAGCAGATAGTAATTTTCAATACAATGCAGCTTTAGGAATATCTAGAGGAATTCTAAATTCT
CTAAATCTATCTGATAATACTAAAGAAAAGCCTATAATAAGCTCTATTAAAAATATAAGTGAGAATATAAGTACAGGTAG
TTCTTATACATTACCTACTTTTGTACAAGCTACTATGAGTAATGGTAGTGTGAAAAAAGTAGGAGTTATATGGGATAATA
AGAATGTAAACACAAGTGTTGCAGGAACGTATACTTATAAAGGAACAGTGGCAGGCTATAATCAACAAATAATACTTACT
TTGAATGTAAAAGGACATAGTACAAATAACAATGTAGTAACCATAGATCCAGGTCACGGTATGGGAAGAGACACAGGTGC
AGTTGGAGGAGTAGTAGAGGATGAAGTTGCATTAAAAGTTGGATTAAAGGTTGGTGCTATACTAGAAAAACATGGAGTAG
ATGTTGTATACACAAGAAAAACAGATCAGCGTTCAAAAAATGGTATGACCGTAAATGAGAGTTTGCAAAAGAGAGTTGAT
ATATCCAATGCAGCTGGTGCAAGATATTTTGTAAGTATACATTGTAATTCTTTTGAGAGTTCTGGTGCTAGTGGAACAGA
AACACTATATAATGTTGGGAACGAAGAAAGTAGAAGACTTGCTTTAGCTATACAAAATAATATAGTAAAAGAAGTTGGAA
CATATAATAGAGGATTAAAAGATGGAAATTGGCTGTATTTAGTTAATAACTCCAAAGCAACAGCTACGGTTTTAGCAGAG
TTAGGGTTTGTCACAAATCCTTCTGATGTGGAAAAATTAAAAAGTGATGAATACTTAGATAAATATGCTAAAGCTATATC
TAATGGAATACTTAAAACTATGGGTAAAATTTAA

Upstream 100 bases:

>100_bases
CATCACATTACATAAATTTTCTTAATAAAATTATACATATTTATATAATATATATTATAATATATATGTTAAGTCTACAG
ATAGGAAAAAGTAATGGAGG

Downstream 100 bases:

>100_bases
ATTTTACCCTTTTCTTAAATTATAAGGAGGAGCAATTATGGGAGTTTTGGTTAAAAATCTAGTAGAAGATTTAAAATTAG
AAGTATTAAATAAAGGCAAA

Product: putative N-acetylmuramoyl-L-alanine amidase

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MIMKNKKKWWFLFVAVAVFTFLGSFNFVKAEEVYDDKGVELNVHSRKSWTVKFNNTLDPATITGDNIVVLNENGGKVPVK
LEIGSKGKTVVVTPSKSYDFEKKYKMILKDGVKSKSGKKLIKSAKLDFTIRNKDDEYKSYTVCIDAGHGGEDSGYVSPYG
LKEKSINLSVALEVGKMLEEKGINVIYTRKDDNIPWSSDEDLNYRFNMANNNKADYFVSIHCNYSDNPIDSGIETYYRDW
DSISQNLSKAVQDEVINNTQGRNRGIKPALPEHEILLGTQAHAIMVNLGFMSNAEETNILADSNFQYNAALGISRGILNS
LNLSDNTKEKPIISSIKNISENISTGSSYTLPTFVQATMSNGSVKKVGVIWDNKNVNTSVAGTYTYKGTVAGYNQQIILT
LNVKGHSTNNNVVTIDPGHGMGRDTGAVGGVVEDEVALKVGLKVGAILEKHGVDVVYTRKTDQRSKNGMTVNESLQKRVD
ISNAAGARYFVSIHCNSFESSGASGTETLYNVGNEESRRLALAIQNNIVKEVGTYNRGLKDGNWLYLVNNSKATATVLAE
LGFVTNPSDVEKLKSDEYLDKYAKAISNGILKTMGKI

Sequences:

>Translated_597_residues
MIMKNKKKWWFLFVAVAVFTFLGSFNFVKAEEVYDDKGVELNVHSRKSWTVKFNNTLDPATITGDNIVVLNENGGKVPVK
LEIGSKGKTVVVTPSKSYDFEKKYKMILKDGVKSKSGKKLIKSAKLDFTIRNKDDEYKSYTVCIDAGHGGEDSGYVSPYG
LKEKSINLSVALEVGKMLEEKGINVIYTRKDDNIPWSSDEDLNYRFNMANNNKADYFVSIHCNYSDNPIDSGIETYYRDW
DSISQNLSKAVQDEVINNTQGRNRGIKPALPEHEILLGTQAHAIMVNLGFMSNAEETNILADSNFQYNAALGISRGILNS
LNLSDNTKEKPIISSIKNISENISTGSSYTLPTFVQATMSNGSVKKVGVIWDNKNVNTSVAGTYTYKGTVAGYNQQIILT
LNVKGHSTNNNVVTIDPGHGMGRDTGAVGGVVEDEVALKVGLKVGAILEKHGVDVVYTRKTDQRSKNGMTVNESLQKRVD
ISNAAGARYFVSIHCNSFESSGASGTETLYNVGNEESRRLALAIQNNIVKEVGTYNRGLKDGNWLYLVNNSKATATVLAE
LGFVTNPSDVEKLKSDEYLDKYAKAISNGILKTMGKI
>Mature_597_residues
MIMKNKKKWWFLFVAVAVFTFLGSFNFVKAEEVYDDKGVELNVHSRKSWTVKFNNTLDPATITGDNIVVLNENGGKVPVK
LEIGSKGKTVVVTPSKSYDFEKKYKMILKDGVKSKSGKKLIKSAKLDFTIRNKDDEYKSYTVCIDAGHGGEDSGYVSPYG
LKEKSINLSVALEVGKMLEEKGINVIYTRKDDNIPWSSDEDLNYRFNMANNNKADYFVSIHCNYSDNPIDSGIETYYRDW
DSISQNLSKAVQDEVINNTQGRNRGIKPALPEHEILLGTQAHAIMVNLGFMSNAEETNILADSNFQYNAALGISRGILNS
LNLSDNTKEKPIISSIKNISENISTGSSYTLPTFVQATMSNGSVKKVGVIWDNKNVNTSVAGTYTYKGTVAGYNQQIILT
LNVKGHSTNNNVVTIDPGHGMGRDTGAVGGVVEDEVALKVGLKVGAILEKHGVDVVYTRKTDQRSKNGMTVNESLQKRVD
ISNAAGARYFVSIHCNSFESSGASGTETLYNVGNEESRRLALAIQNNIVKEVGTYNRGLKDGNWLYLVNNSKATATVLAE
LGFVTNPSDVEKLKSDEYLDKYAKAISNGILKTMGKI

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=259, Percent_Identity=26.2548262548263, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 65702; Mature: 65702

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMKNKKKWWFLFVAVAVFTFLGSFNFVKAEEVYDDKGVELNVHSRKSWTVKFNNTLDPA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCEEEEEEECCCCCCC
TITGDNIVVLNENGGKVPVKLEIGSKGKTVVVTPSKSYDFEKKYKMILKDGVKSKSGKKL
EECCCEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHH
IKSAKLDFTIRNKDDEYKSYTVCIDAGHGGEDSGYVSPYGLKEKSINLSVALEVGKMLEE
HHHCCEEEEECCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCEEEEEEEHHHHHHHH
KGINVIYTRKDDNIPWSSDEDLNYRFNMANNNKADYFVSIHCNYSDNPIDSGIETYYRDW
CCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHCH
DSISQNLSKAVQDEVINNTQGRNRGIKPALPEHEILLGTQAHAIMVNLGFMSNAEETNIL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCEEE
ADSNFQYNAALGISRGILNSLNLSDNTKEKPIISSIKNISENISTGSSYTLPTFVQATMS
ECCCCEEEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEECC
NGSVKKVGVIWDNKNVNTSVAGTYTYKGTVAGYNQQIILTLNVKGHSTNNNVVTIDPGHG
CCCEEEEEEEECCCCCCCEEEEEEEEECEEECCCCEEEEEEEEECCCCCCCEEEECCCCC
MGRDTGAVGGVVEDEVALKVGLKVGAILEKHGVDVVYTRKTDQRSKNGMTVNESLQKRVD
CCCCCCCCCCEECCCEEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCC
ISNAAGARYFVSIHCNSFESSGASGTETLYNVGNEESRRLALAIQNNIVKEVGTYNRGLK
CCCCCCCEEEEEEEECCCCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCC
DGNWLYLVNNSKATATVLAELGFVTNPSDVEKLKSDEYLDKYAKAISNGILKTMGKI
CCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIMKNKKKWWFLFVAVAVFTFLGSFNFVKAEEVYDDKGVELNVHSRKSWTVKFNNTLDPA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCEEEEEEECCCCCCC
TITGDNIVVLNENGGKVPVKLEIGSKGKTVVVTPSKSYDFEKKYKMILKDGVKSKSGKKL
EECCCEEEEECCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHH
IKSAKLDFTIRNKDDEYKSYTVCIDAGHGGEDSGYVSPYGLKEKSINLSVALEVGKMLEE
HHHCCEEEEECCCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCEEEEEEEHHHHHHHH
KGINVIYTRKDDNIPWSSDEDLNYRFNMANNNKADYFVSIHCNYSDNPIDSGIETYYRDW
CCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHCH
DSISQNLSKAVQDEVINNTQGRNRGIKPALPEHEILLGTQAHAIMVNLGFMSNAEETNIL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCEEE
ADSNFQYNAALGISRGILNSLNLSDNTKEKPIISSIKNISENISTGSSYTLPTFVQATMS
ECCCCEEEEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEECC
NGSVKKVGVIWDNKNVNTSVAGTYTYKGTVAGYNQQIILTLNVKGHSTNNNVVTIDPGHG
CCCEEEEEEEECCCCCCCEEEEEEEEECEEECCCCEEEEEEEEECCCCCCCEEEECCCCC
MGRDTGAVGGVVEDEVALKVGLKVGAILEKHGVDVVYTRKTDQRSKNGMTVNESLQKRVD
CCCCCCCCCCEECCCEEEEECCHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHCC
ISNAAGARYFVSIHCNSFESSGASGTETLYNVGNEESRRLALAIQNNIVKEVGTYNRGLK
CCCCCCCEEEEEEEECCCCCCCCCCCHHHHHCCCCCCCEEEEEEHHHHHHHHHHHCCCCC
DGNWLYLVNNSKATATVLAELGFVTNPSDVEKLKSDEYLDKYAKAISNGILKTMGKI
CCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]