Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 28210690

GI number: 28210690

Start: 1052080

End: 1054176

Strand: Direct

Name: pcrA [H]

Synonym: CTC00984

Alternate gene names: 28210690

Gene position: 1052080-1054176 (Clockwise)

Preceding gene: 28210688

Following gene: 28210691

Centisome position: 37.58

GC content: 22.75

Gene sequence:

>2097_bases
TTGGCAATAATTTTAATGTTAATAATTATTATTTTACCCCCATATATGTTATATTACAAGTTTATTATATTGGAAAGGAG
ACAGAAGAGCTTGAAAGAAATAAGATTAGACAAATATCAAATGGAAGCTGTAAAAAGCAATCATAGAAACTTAGTAGTGA
TTGCACCTCCAGGAAGCGGTAAAACTTTTGTTATTATAAATAGAATATTTTATCTAATGAAAGAGAGGGGTGTATGTGGA
AAGAATATCTTTGCCATGACCTTTACTAAAAGTGCAGCTAAAGATATGGAGAGAAGATTTTTGATATTAAAAAAAGCTAA
TAATTATTATTCTATGCATACACCATTTTTTGGAACATTACACAGCTTATTTTATAATATATTAAGAACTTATTGTGGAG
TTATAAATATAATTTCGGAAAAAGAAAGTATATTTATAATAGAGAAAGTTTTAAAAAAGTACACTAATAATATAAGAGAA
GAATTAGTTAAAGATGTATTAAATAATATATCAAAATATAAAAGTAAACAGCGAATAGATAATCATATAGATGAAAAGTT
ATTTTATGAATGCTATGATGAATACGAAAGATATAAAAGTTTAAAAAATAAATTAGATTTTGATGATATACAAATAAAAT
GCAGAGAACTTTTAGTAAACAATAGTGAAATTTTAAATGAATGTAGAAATAAATTTAAGTATTTATTAATAGATGAGTTT
CAAGATTGTGATCATATGCAGTTAAGTATATTAAAGATGTTAAATAAAGATAATAGTATTTTTGCAGTGGGAGATGAAGA
TCAATGCATCTATAGTTTTAGAGGGGCAAAACCAGAAGGTATGGTAGATTTTCCTAGGTATTTTCATCAAGGGGAAAAAA
TATTTTTAAAAATTAATTATAGAAGTGGAGAAAATATAGTAGAATTATCAAAGAACTTAATTAAAATTAATAAACTAAGA
AGCTCTAAAGAAATATTCACTTATAATAAATTTGAAGGAAAAATAAATATAATATATAGTGAAGATGAAAAGGAACAAAT
AGAGAATATTTTAAAAGAAGTACTTTATAATATAGAGAAAAAAGGGTATAATTTCAAAGACATAGCTATACTTTATAGAA
GAAATACAGAGGCACTTATAATTATAGATTATTTCCTAAGAAATAATGTGGAGTTTAATCTTAATCAGAAATATTTTAAT
ATATATAATCATTTCATATGTAATGATATACTAAGCTATATAAAACTTTCATTAGATAATAAAAATAGACATAGTTTTAC
TAGAATAATAAATAAACCATTTAGATATATAAGTAAAACACATATAGAAAAACTAAAAGGATTTAGAAGAAAGGATAATT
GCTTTGATATAATGTGCAATTTAAATATATCATTATTACAAATAAAGACTATTAAAGCTTTAAAAAGAAATATAAATAAA
ATAAAGAAACTTGATATAGATAAGATTGTAGATTACATAATGAAAGATATAGGATATGAGGAATATATACATAGCTACTC
AAAAAAACTAAAAATAGATGAAGAAGAGCTTTTGTATATAGTTTATGTTTTAAAGAAGGCATTAGAAGATTTTACTTCAT
TAGAGGATTTTATAAGATATATAGAGGAAAGTAAAAATAAAGCTTTAGAAAAAAAGGAAAATGCCATAACTTTGAGTAGT
ATTCATAGTTCTAAAGGAATGGAATTTAAAAGTGTATTTATAATAAACTGCAATGATGGAAATATACCACATAATAGTAA
TGTAAATGATTTAGAAGATAAATTTAAATTAGAGGAAGAAAGACGATTGTTTTATGTTGCTTTAACTCGAAGTATTTCTA
ATTTAAATTTATGCATATGTAGTAATATAAGAGGCAAATATTTACCTTATTCACGATTCTTAAAGGAATGTAATTTAAAT
TTGAAAGATAGTTATAACACAGATGAAGTAGTATTGCATAAATATTTAGGAGAAGGAAAAATTCGAAAAGTAAAAGAAAA
TAAGATAATCATAGATTTTAATGGAATATGTAGAATGCTTAAATTAGATGTAATAAATATAAATGGATTAATTCAAAAAG
TGACAAAAGATGCTTAA

Upstream 100 bases:

>100_bases
AGAAGTCTGCTAACATAGTTAAATTTCTATTACTTCTTATGTCGAAATTTTTTTTATACTCCATCTATATCCTCCATATT
AAGTTTTATTTTTTTTATTT

Downstream 100 bases:

>100_bases
AAGTATCTTTTATTGAATATGTTTAAATTATATTATATAATCTTATTAAGCTCATATTCTAGGAATATGAGCTTTTAAAT
TGTTTTAAAAGAGGTAGAGT

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MAIILMLIIIILPPYMLYYKFIILERRQKSLKEIRLDKYQMEAVKSNHRNLVVIAPPGSGKTFVIINRIFYLMKERGVCG
KNIFAMTFTKSAAKDMERRFLILKKANNYYSMHTPFFGTLHSLFYNILRTYCGVINIISEKESIFIIEKVLKKYTNNIRE
ELVKDVLNNISKYKSKQRIDNHIDEKLFYECYDEYERYKSLKNKLDFDDIQIKCRELLVNNSEILNECRNKFKYLLIDEF
QDCDHMQLSILKMLNKDNSIFAVGDEDQCIYSFRGAKPEGMVDFPRYFHQGEKIFLKINYRSGENIVELSKNLIKINKLR
SSKEIFTYNKFEGKINIIYSEDEKEQIENILKEVLYNIEKKGYNFKDIAILYRRNTEALIIIDYFLRNNVEFNLNQKYFN
IYNHFICNDILSYIKLSLDNKNRHSFTRIINKPFRYISKTHIEKLKGFRRKDNCFDIMCNLNISLLQIKTIKALKRNINK
IKKLDIDKIVDYIMKDIGYEEYIHSYSKKLKIDEEELLYIVYVLKKALEDFTSLEDFIRYIEESKNKALEKKENAITLSS
IHSSKGMEFKSVFIINCNDGNIPHNSNVNDLEDKFKLEEERRLFYVALTRSISNLNLCICSNIRGKYLPYSRFLKECNLN
LKDSYNTDEVVLHKYLGEGKIRKVKENKIIIDFNGICRMLKLDVININGLIQKVTKDA

Sequences:

>Translated_698_residues
MAIILMLIIIILPPYMLYYKFIILERRQKSLKEIRLDKYQMEAVKSNHRNLVVIAPPGSGKTFVIINRIFYLMKERGVCG
KNIFAMTFTKSAAKDMERRFLILKKANNYYSMHTPFFGTLHSLFYNILRTYCGVINIISEKESIFIIEKVLKKYTNNIRE
ELVKDVLNNISKYKSKQRIDNHIDEKLFYECYDEYERYKSLKNKLDFDDIQIKCRELLVNNSEILNECRNKFKYLLIDEF
QDCDHMQLSILKMLNKDNSIFAVGDEDQCIYSFRGAKPEGMVDFPRYFHQGEKIFLKINYRSGENIVELSKNLIKINKLR
SSKEIFTYNKFEGKINIIYSEDEKEQIENILKEVLYNIEKKGYNFKDIAILYRRNTEALIIIDYFLRNNVEFNLNQKYFN
IYNHFICNDILSYIKLSLDNKNRHSFTRIINKPFRYISKTHIEKLKGFRRKDNCFDIMCNLNISLLQIKTIKALKRNINK
IKKLDIDKIVDYIMKDIGYEEYIHSYSKKLKIDEEELLYIVYVLKKALEDFTSLEDFIRYIEESKNKALEKKENAITLSS
IHSSKGMEFKSVFIINCNDGNIPHNSNVNDLEDKFKLEEERRLFYVALTRSISNLNLCICSNIRGKYLPYSRFLKECNLN
LKDSYNTDEVVLHKYLGEGKIRKVKENKIIIDFNGICRMLKLDVININGLIQKVTKDA
>Mature_697_residues
AIILMLIIIILPPYMLYYKFIILERRQKSLKEIRLDKYQMEAVKSNHRNLVVIAPPGSGKTFVIINRIFYLMKERGVCGK
NIFAMTFTKSAAKDMERRFLILKKANNYYSMHTPFFGTLHSLFYNILRTYCGVINIISEKESIFIIEKVLKKYTNNIREE
LVKDVLNNISKYKSKQRIDNHIDEKLFYECYDEYERYKSLKNKLDFDDIQIKCRELLVNNSEILNECRNKFKYLLIDEFQ
DCDHMQLSILKMLNKDNSIFAVGDEDQCIYSFRGAKPEGMVDFPRYFHQGEKIFLKINYRSGENIVELSKNLIKINKLRS
SKEIFTYNKFEGKINIIYSEDEKEQIENILKEVLYNIEKKGYNFKDIAILYRRNTEALIIIDYFLRNNVEFNLNQKYFNI
YNHFICNDILSYIKLSLDNKNRHSFTRIINKPFRYISKTHIEKLKGFRRKDNCFDIMCNLNISLLQIKTIKALKRNINKI
KKLDIDKIVDYIMKDIGYEEYIHSYSKKLKIDEEELLYIVYVLKKALEDFTSLEDFIRYIEESKNKALEKKENAITLSSI
HSSKGMEFKSVFIINCNDGNIPHNSNVNDLEDKFKLEEERRLFYVALTRSISNLNLCICSNIRGKYLPYSRFLKECNLNL
KDSYNTDEVVLHKYLGEGKIRKVKENKIIIDFNGICRMLKLDVININGLIQKVTKDA

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=666, Percent_Identity=27.027027027027, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI48994965, Length=631, Percent_Identity=26.7828843106181, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322369, Length=468, Percent_Identity=26.7094017094017, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324477, Length=358, Percent_Identity=26.536312849162, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82983; Mature: 82852

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIILMLIIIILPPYMLYYKFIILERRQKSLKEIRLDKYQMEAVKSNHRNLVVIAPPGSG
CHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
KTFVIINRIFYLMKERGVCGKNIFAMTFTKSAAKDMERRFLILKKANNYYSMHTPFFGTL
CEEEHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHEEEEEECCCEEEECCCHHHHH
HSLFYNILRTYCGVINIISEKESIFIIEKVLKKYTNNIREELVKDVLNNISKYKSKQRID
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHIDEKLFYECYDEYERYKSLKNKLDFDDIQIKCRELLVNNSEILNECRNKFKYLLIDEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECC
QDCDHMQLSILKMLNKDNSIFAVGDEDQCIYSFRGAKPEGMVDFPRYFHQGEKIFLKINY
CCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEEE
RSGENIVELSKNLIKINKLRSSKEIFTYNKFEGKINIIYSEDEKEQIENILKEVLYNIEK
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHH
KGYNFKDIAILYRRNTEALIIIDYFLRNNVEFNLNQKYFNIYNHFICNDILSYIKLSLDN
CCCCEEEEEEEEECCCCEEEEEEEHHHCCCEEECCHHHHHHHHHHHHHHHHHHHEECCCC
KNRHSFTRIINKPFRYISKTHIEKLKGFRRKDNCFDIMCNLNISLLQIKTIKALKRNINK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEHHHHHHHHHHHHH
IKKLDIDKIVDYIMKDIGYEEYIHSYSKKLKIDEEELLYIVYVLKKALEDFTSLEDFIRY
HHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEESKNKALEKKENAITLSSIHSSKGMEFKSVFIINCNDGNIPHNSNVNDLEDKFKLEEE
HHHHHHHHHHHHCCEEEEEHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHCHHH
RRLFYVALTRSISNLNLCICSNIRGKYLPYSRFLKECNLNLKDSYNTDEVVLHKYLGEGK
HEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
IRKVKENKIIIDFNGICRMLKLDVININGLIQKVTKDA
EEEEECCEEEEEEHHHHHHHHHEEEEHHHHHHHHHCCC
>Mature Secondary Structure 
AIILMLIIIILPPYMLYYKFIILERRQKSLKEIRLDKYQMEAVKSNHRNLVVIAPPGSG
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
KTFVIINRIFYLMKERGVCGKNIFAMTFTKSAAKDMERRFLILKKANNYYSMHTPFFGTL
CEEEHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHEEEEEECCCEEEECCCHHHHH
HSLFYNILRTYCGVINIISEKESIFIIEKVLKKYTNNIREELVKDVLNNISKYKSKQRID
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NHIDEKLFYECYDEYERYKSLKNKLDFDDIQIKCRELLVNNSEILNECRNKFKYLLIDEF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHEEEEEECC
QDCDHMQLSILKMLNKDNSIFAVGDEDQCIYSFRGAKPEGMVDFPRYFHQGEKIFLKINY
CCCCHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEEE
RSGENIVELSKNLIKINKLRSSKEIFTYNKFEGKINIIYSEDEKEQIENILKEVLYNIEK
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHH
KGYNFKDIAILYRRNTEALIIIDYFLRNNVEFNLNQKYFNIYNHFICNDILSYIKLSLDN
CCCCEEEEEEEEECCCCEEEEEEEHHHCCCEEECCHHHHHHHHHHHHHHHHHHHEECCCC
KNRHSFTRIINKPFRYISKTHIEKLKGFRRKDNCFDIMCNLNISLLQIKTIKALKRNINK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEHHHHHHHHHHHHH
IKKLDIDKIVDYIMKDIGYEEYIHSYSKKLKIDEEELLYIVYVLKKALEDFTSLEDFIRY
HHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEESKNKALEKKENAITLSSIHSSKGMEFKSVFIINCNDGNIPHNSNVNDLEDKFKLEEE
HHHHHHHHHHHHCCEEEEEHHHCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHCHHH
RRLFYVALTRSISNLNLCICSNIRGKYLPYSRFLKECNLNLKDSYNTDEVVLHKYLGEGK
HEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
IRKVKENKIIIDFNGICRMLKLDVININGLIQKVTKDA
EEEEECCEEEEEEHHHHHHHHHEEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA