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The map label for this gene is pflD [H]

Identifier: 28210647

GI number: 28210647

Start: 996725

End: 999136

Strand: Direct

Name: pflD [H]

Synonym: CTC00936

Alternate gene names: 28210647

Gene position: 996725-999136 (Clockwise)

Preceding gene: 28210646

Following gene: 28210648

Centisome position: 35.61

GC content: 32.5

Gene sequence:

>2412_bases
ATGAGTTTTTTAGAAAAAGGATTTAGTGAATCAACAGACAGAGTGAAAAGGTTAAAAGAGCAGATATTTTCTGCAGTTCC
TTGTGTTGAAGCAGACAGAGCTATACTGTTAACGGAATCATTTAAAGAAACTGAAGATCAACCTATTATTATTCGTAAAG
CTAAAGCATTGGAAAAGATTTTAAATGAAATTCCTATTGTAATTAGAGATGGGGAACTTATAGTTGGAAGCTTAACTAAA
AATCCAAGATCAGCACAAGTTTTCCCTGAGTTTTCTAATAAGTGGTTAGTAGAAGAATTTGATAAACTTAATAAGAGAAC
ATCCGATGCTTTTGAGATTTCAGATGAAGCAAAGTCACAACTTATAGAAGTATTTAAATATTGGGAAGGAAAAACTGTAA
GTGAGCTTGCTACATCATATATGACCGATGATACTATTGATGCAATGAATGCAAAAGTATTTACAGTTGCAAACTATCAT
TTTAATGGATTAGGACATATTTCTGTAGATTATGAAAAAGTTCTTAAAAAAGGGTTTAGCGGAATTATTAAAGAAGTTAA
TGAAACCATTAATAATGCTGATAAAAATAATCCTGAATATATTAAGCAAAGAAGGTTTTGGGACGCTATAATTATTTCCT
GTGAGGCAGCAATAAGTTATGCAAAAAGATATTCACAACTTGCAAAAGAATTAGCACAAAAAGAAACAAATGCTACTCGA
AAAGATGAATTGTTAAATATATCTAAAATATGTGAAAAAGTTCCGGAAAAGCCAGCAACAACATTTCAAGAAGCATGTCA
ATCTTTTTGGTTTGTTCAAGCAATTATAAGCTTAGAATCAAACGGACATGCTATATCTCCAGCTCGTTTTGACCAATATA
TGTACCCATATTTTAAACATGATATAGATTTACACCCTAAAAATAGAGAATCAGCTAGAGAAATTCTTCATTGCTTATGG
GTTAAATTTAATGATTTAACTAAAGTTCGTGACGAAACTACAACCAAAGCTTTTAGTGGATATTCAATGTTTCAAAATCT
AATTGTAGGAGGACAGACTCCAGAAGGTAAGGATGCTACTAATGAATTATCATATATGTGTATGGATGCCACTGGAACTT
TGATGTTACCTCAACCTTCACTTTCAGTTAGAATATGGAGTAAAGCTCCTGAAGAATTTTTAATAAGAAGCTGTGAGTTA
ACACGTTTAGGCACAGGACTTCCTGCTTTCTATAATGATGAAGTTATTATACCTATGTTGGTTAATCATGGATTAACACT
AGAAGATGCTAGAGATTATGCAATTGTAGGATGTGTTGAACCTCAAAAACCTGGTAAAACAGATGGATGGTACGATGCAG
CATTTTTTAATATGGCGAAAGTCCTTGAAATGACTATAAATAATGGTAAAGTTGATGGAAAACAAATAGGACCTAAAACA
GGTGAATTTACTTCATTTAGTAATATAGACGAATTTATAGAAGCTTACAAAAAGCAAACAGAATATTTTGTGTCTTACAT
GGTTTCAGCAGATAATTGTGTTGATATTGCACATGCTGAAAGAGCACCACTTCCGTTTTTATCATGTATGCTTGATGATT
GTATATCAAAAGGAAAATCAATACAAGAAGGTGGAGGACACTATCGTTTCTCAGGACCTTTAGGTGTTGGTATTGCTAAT
ATAGGAGATTCATTTATGGCTATTAAAAAGCTTGTGTTTGATGAGAAAAAGCTTACTTTAAATGAGATAAAAGAAGCTAT
AGATACCAATTATGGTGAGTTTGAAGAGAACCCAGAGAAAAAGATAAAATATAAGGATATTCAACAAATGCTTTTAAATA
GAGCTCCTAAGTTTGGAAATGATATAGATGAAGTTGATGAATATACAAGATTAGGTGCACTAATTTATTGTAAAGAAGTA
GAAAAGCATACAAATCAACGTGGAGGACAATTTATACCAGGCTTATACCCAGTTTCAAATAATGTACATCTAGGAACTTT
AGTATCAGCAACACCTGATGGAAGAGGAGCCAAGAAGCCTTTAGCAGATGGAGTATCTCCAACTCGTGGAGTTGATGTTA
ATGGACCAACTGCTGCTGCAAATTCTGTAGCAAAATTAGAGCATTTTTGTGCACCAAGTGGAACTTTATTTAATCAAAAG
TTTAGTCCAAGTTCCCTTCAAGGGGATAATGGATTGAAGAATCTTGCTTCTTTAATACGTAGCTATTTTGACCAAAAAGG
TATGCACATGCAATTCAATGTAGTTGATAAAAATGTTCTTATTGATGCACAAAAACGTCCGGAACAGTATAGAGATTTAA
TAGTTCGTGTTGCAGGATATAGTGCTCAATTTATTTGCCTAGATAATGCAATTCAGGACGATATAATTAAAAGAACAGAA
CAAAGTTTTTAA

Upstream 100 bases:

>100_bases
TGGTACTATATAAGTATGGTATTATCAAGCTATTTTAATTTTATTAATTGTGATTAATTCTTAAAAAGGATTAAATTATT
ATAAAAATAGGGGGATAATT

Downstream 100 bases:

>100_bases
TATATAAGTACTAATTTAAGGCTTGATTATATTCAAGCCTTAAATTTAGTCATTTTATAGAGGAGGGTACTCTATGGTAA
AAAAGCAAGTGGATTATAAT

Product: formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MSFLEKGFSESTDRVKRLKEQIFSAVPCVEADRAILLTESFKETEDQPIIIRKAKALEKILNEIPIVIRDGELIVGSLTK
NPRSAQVFPEFSNKWLVEEFDKLNKRTSDAFEISDEAKSQLIEVFKYWEGKTVSELATSYMTDDTIDAMNAKVFTVANYH
FNGLGHISVDYEKVLKKGFSGIIKEVNETINNADKNNPEYIKQRRFWDAIIISCEAAISYAKRYSQLAKELAQKETNATR
KDELLNISKICEKVPEKPATTFQEACQSFWFVQAIISLESNGHAISPARFDQYMYPYFKHDIDLHPKNRESAREILHCLW
VKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPEGKDATNELSYMCMDATGTLMLPQPSLSVRIWSKAPEEFLIRSCEL
TRLGTGLPAFYNDEVIIPMLVNHGLTLEDARDYAIVGCVEPQKPGKTDGWYDAAFFNMAKVLEMTINNGKVDGKQIGPKT
GEFTSFSNIDEFIEAYKKQTEYFVSYMVSADNCVDIAHAERAPLPFLSCMLDDCISKGKSIQEGGGHYRFSGPLGVGIAN
IGDSFMAIKKLVFDEKKLTLNEIKEAIDTNYGEFEENPEKKIKYKDIQQMLLNRAPKFGNDIDEVDEYTRLGALIYCKEV
EKHTNQRGGQFIPGLYPVSNNVHLGTLVSATPDGRGAKKPLADGVSPTRGVDVNGPTAAANSVAKLEHFCAPSGTLFNQK
FSPSSLQGDNGLKNLASLIRSYFDQKGMHMQFNVVDKNVLIDAQKRPEQYRDLIVRVAGYSAQFICLDNAIQDDIIKRTE
QSF

Sequences:

>Translated_803_residues
MSFLEKGFSESTDRVKRLKEQIFSAVPCVEADRAILLTESFKETEDQPIIIRKAKALEKILNEIPIVIRDGELIVGSLTK
NPRSAQVFPEFSNKWLVEEFDKLNKRTSDAFEISDEAKSQLIEVFKYWEGKTVSELATSYMTDDTIDAMNAKVFTVANYH
FNGLGHISVDYEKVLKKGFSGIIKEVNETINNADKNNPEYIKQRRFWDAIIISCEAAISYAKRYSQLAKELAQKETNATR
KDELLNISKICEKVPEKPATTFQEACQSFWFVQAIISLESNGHAISPARFDQYMYPYFKHDIDLHPKNRESAREILHCLW
VKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPEGKDATNELSYMCMDATGTLMLPQPSLSVRIWSKAPEEFLIRSCEL
TRLGTGLPAFYNDEVIIPMLVNHGLTLEDARDYAIVGCVEPQKPGKTDGWYDAAFFNMAKVLEMTINNGKVDGKQIGPKT
GEFTSFSNIDEFIEAYKKQTEYFVSYMVSADNCVDIAHAERAPLPFLSCMLDDCISKGKSIQEGGGHYRFSGPLGVGIAN
IGDSFMAIKKLVFDEKKLTLNEIKEAIDTNYGEFEENPEKKIKYKDIQQMLLNRAPKFGNDIDEVDEYTRLGALIYCKEV
EKHTNQRGGQFIPGLYPVSNNVHLGTLVSATPDGRGAKKPLADGVSPTRGVDVNGPTAAANSVAKLEHFCAPSGTLFNQK
FSPSSLQGDNGLKNLASLIRSYFDQKGMHMQFNVVDKNVLIDAQKRPEQYRDLIVRVAGYSAQFICLDNAIQDDIIKRTE
QSF
>Mature_802_residues
SFLEKGFSESTDRVKRLKEQIFSAVPCVEADRAILLTESFKETEDQPIIIRKAKALEKILNEIPIVIRDGELIVGSLTKN
PRSAQVFPEFSNKWLVEEFDKLNKRTSDAFEISDEAKSQLIEVFKYWEGKTVSELATSYMTDDTIDAMNAKVFTVANYHF
NGLGHISVDYEKVLKKGFSGIIKEVNETINNADKNNPEYIKQRRFWDAIIISCEAAISYAKRYSQLAKELAQKETNATRK
DELLNISKICEKVPEKPATTFQEACQSFWFVQAIISLESNGHAISPARFDQYMYPYFKHDIDLHPKNRESAREILHCLWV
KFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPEGKDATNELSYMCMDATGTLMLPQPSLSVRIWSKAPEEFLIRSCELT
RLGTGLPAFYNDEVIIPMLVNHGLTLEDARDYAIVGCVEPQKPGKTDGWYDAAFFNMAKVLEMTINNGKVDGKQIGPKTG
EFTSFSNIDEFIEAYKKQTEYFVSYMVSADNCVDIAHAERAPLPFLSCMLDDCISKGKSIQEGGGHYRFSGPLGVGIANI
GDSFMAIKKLVFDEKKLTLNEIKEAIDTNYGEFEENPEKKIKYKDIQQMLLNRAPKFGNDIDEVDEYTRLGALIYCKEVE
KHTNQRGGQFIPGLYPVSNNVHLGTLVSATPDGRGAKKPLADGVSPTRGVDVNGPTAAANSVAKLEHFCAPSGTLFNQKF
SPSSLQGDNGLKNLASLIRSYFDQKGMHMQFNVVDKNVLIDAQKRPEQYRDLIVRVAGYSAQFICLDNAIQDDIIKRTEQ
SF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=794, Percent_Identity=39.168765743073, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1787044, Length=810, Percent_Identity=36.0493827160494, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI48994926, Length=657, Percent_Identity=24.9619482496195, Blast_Score=161, Evalue=2e-40,
Organism=Escherichia coli, GI1787131, Length=560, Percent_Identity=26.9642857142857, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90345; Mature: 90213

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFLEKGFSESTDRVKRLKEQIFSAVPCVEADRAILLTESFKETEDQPIIIRKAKALEKI
CCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCCCCCEEEHHHHHHHHH
LNEIPIVIRDGELIVGSLTKNPRSAQVFPEFSNKWLVEEFDKLNKRTSDAFEISDEAKSQ
HHHCCEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCHHHHHH
LIEVFKYWEGKTVSELATSYMTDDTIDAMNAKVFTVANYHFNGLGHISVDYEKVLKKGFS
HHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCEEEEEEEEEECCCEEEECCHHHHHHHHHH
GIIKEVNETINNADKNNPEYIKQRRFWDAIIISCEAAISYAKRYSQLAKELAQKETNATR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KDELLNISKICEKVPEKPATTFQEACQSFWFVQAIISLESNGHAISPARFDQYMYPYFKH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHC
DIDLHPKNRESAREILHCLWVKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPEGKDAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
NELSYMCMDATGTLMLPQPSLSVRIWSKAPEEFLIRSCELTRLGTGLPAFYNDEVIIPML
HHHHEEEECCCCEEEECCCCCEEEEECCCCHHHHHHHCCHHHCCCCCCCCCCCCEEEEHH
VNHGLTLEDARDYAIVGCVEPQKPGKTDGWYDAAFFNMAKVLEMTINNGKVDGKQIGPKT
HCCCCEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCC
GEFTSFSNIDEFIEAYKKQTEYFVSYMVSADNCVDIAHAERAPLPFLSCMLDDCISKGKS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCHHHHHHHHHHHHCCCC
IQEGGGHYRFSGPLGVGIANIGDSFMAIKKLVFDEKKLTLNEIKEAIDTNYGEFEENPEK
CCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHH
KIKYKDIQQMLLNRAPKFGNDIDEVDEYTRLGALIYCKEVEKHTNQRGGQFIPGLYPVSN
HCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC
NVHLGTLVSATPDGRGAKKPLADGVSPTRGVDVNGPTAAANSVAKLEHFCAPSGTLFNQK
CEEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FSPSSLQGDNGLKNLASLIRSYFDQKGMHMQFNVVDKNVLIDAQKRPEQYRDLIVRVAGY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHCCC
SAQFICLDNAIQDDIIKRTEQSF
CEEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFLEKGFSESTDRVKRLKEQIFSAVPCVEADRAILLTESFKETEDQPIIIRKAKALEKI
CHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCCCCCEEEHHHHHHHHH
LNEIPIVIRDGELIVGSLTKNPRSAQVFPEFSNKWLVEEFDKLNKRTSDAFEISDEAKSQ
HHHCCEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCHHHHHH
LIEVFKYWEGKTVSELATSYMTDDTIDAMNAKVFTVANYHFNGLGHISVDYEKVLKKGFS
HHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCEEEEEEEEEECCCEEEECCHHHHHHHHHH
GIIKEVNETINNADKNNPEYIKQRRFWDAIIISCEAAISYAKRYSQLAKELAQKETNATR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KDELLNISKICEKVPEKPATTFQEACQSFWFVQAIISLESNGHAISPARFDQYMYPYFKH
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHC
DIDLHPKNRESAREILHCLWVKFNDLTKVRDETTTKAFSGYSMFQNLIVGGQTPEGKDAT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
NELSYMCMDATGTLMLPQPSLSVRIWSKAPEEFLIRSCELTRLGTGLPAFYNDEVIIPML
HHHHEEEECCCCEEEECCCCCEEEEECCCCHHHHHHHCCHHHCCCCCCCCCCCCEEEEHH
VNHGLTLEDARDYAIVGCVEPQKPGKTDGWYDAAFFNMAKVLEMTINNGKVDGKQIGPKT
HCCCCEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCC
GEFTSFSNIDEFIEAYKKQTEYFVSYMVSADNCVDIAHAERAPLPFLSCMLDDCISKGKS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHCCCCCCHHHHHHHHHHHHCCCC
IQEGGGHYRFSGPLGVGIANIGDSFMAIKKLVFDEKKLTLNEIKEAIDTNYGEFEENPEK
CCCCCCEEEECCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHH
KIKYKDIQQMLLNRAPKFGNDIDEVDEYTRLGALIYCKEVEKHTNQRGGQFIPGLYPVSN
HCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCC
NVHLGTLVSATPDGRGAKKPLADGVSPTRGVDVNGPTAAANSVAKLEHFCAPSGTLFNQK
CEEEEEEEECCCCCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FSPSSLQGDNGLKNLASLIRSYFDQKGMHMQFNVVDKNVLIDAQKRPEQYRDLIVRVAGY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHCCC
SAQFICLDNAIQDDIIKRTEQSF
CEEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]