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The map label for this gene is purR [H]

Identifier: 28210615

GI number: 28210615

Start: 957857

End: 958888

Strand: Direct

Name: purR [H]

Synonym: CTC00904

Alternate gene names: 28210615

Gene position: 957857-958888 (Clockwise)

Preceding gene: 28210614

Following gene: 28210616

Centisome position: 34.22

GC content: 30.62

Gene sequence:

>1032_bases
ATGGGGTATATGGAGGGAATTATGAGTAAAATAACAATAAAAGAAGTAGCGAAAAGAGCAAATGTATCTACGGCAACTGT
ATCTAGAGTTTTGAATAATAACTATCCCGTAAGTGATGAAGCAAGAAAGGAAGTTTTGAAGGCTGCCTCAGAACTGAATT
ATAAGCCAAATGGAGTAGCAAGAAGTCTTAAGAAGAATAAGACAAACTTAATAGGTATTATCGTTGCAGATATATCAAAT
CCATTTTTCATGAGTGTGGCAAGAGGAGTAGAAAGTGTAGTTTCAGAAGAATACAATCTTATTTTATCTAGTACAGATGA
ATCACCAGATAAGGAAGTAAAATTATTAAATATGATGCTTGAAAAAAAAGTAGACGGAATAGTAATAGCATCATCCAATA
TTGATTCATCAGCAATAAAAAGCATAATAGCAAGTGGCATTAAATTAGTATTAATTGATAGAAAAGTAGATGATGTTACT
TTAGATTATATTGCTAATGATAATTTCAATGGTTCATTTTTACTTACAGAGAGCTTAATAAAGAAAGGGCACAAAGATAT
CGCAATAGTAAACGGTTGCTTGAACGTAACCACTGCAATAGAAAGATTTAATGGATATAGAGCTGCTATGAATAAGTATG
GTCTTAAAATAGATAAAGACTTTATATTGCAAGGAAATTACAGTGCTAGTATTGCATTTGTTGAAACTAGAAATTTGATA
AGGAAAGGAAAGCTTCCAACTGCTTTATTATCAGCCAATAATAGTATGACAGAGGGAATCATGAAAGCACTTAAAAGTGA
AAAAGTAAGAATTCCTGAAGACATCTCATTGGTATCATTTGGAGAAATAACCAATCAAGAATTTATAGAACCAAAAATCA
CATGTATAAAACAGAATCCTTTTCTTATGGGACAAAAAGCAGGAGAAGTGATTTTAGATAAGCTAAATGGTAAAAGTAAA
GAGGATAATTTTAAAGAAGTAGTATTGATTAACGAATTCTGTGAAGGGAATTCTATTAAATCTTTAATATAG

Upstream 100 bases:

>100_bases
AGAGAATAATATAAGATTAGCAATAGCAACCGGTGAGAAAAGAATATTTGCTAATATATTACTTACAGTTGGAGTTGTGT
AGTTAAAATTTTTAGATCGT

Downstream 100 bases:

>100_bases
ACTAATTTATTAAAATCATTAAGATATAATTTAATTCCTTTTATAAGGATAAGTATTTTATTTATAGTAAAGGATTACTA
TATTTTAAAGAGGTGAGTTT

Product: ribose operon repressor

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MGYMEGIMSKITIKEVAKRANVSTATVSRVLNNNYPVSDEARKEVLKAASELNYKPNGVARSLKKNKTNLIGIIVADISN
PFFMSVARGVESVVSEEYNLILSSTDESPDKEVKLLNMMLEKKVDGIVIASSNIDSSAIKSIIASGIKLVLIDRKVDDVT
LDYIANDNFNGSFLLTESLIKKGHKDIAIVNGCLNVTTAIERFNGYRAAMNKYGLKIDKDFILQGNYSASIAFVETRNLI
RKGKLPTALLSANNSMTEGIMKALKSEKVRIPEDISLVSFGEITNQEFIEPKITCIKQNPFLMGQKAGEVILDKLNGKSK
EDNFKEVVLINEFCEGNSIKSLI

Sequences:

>Translated_343_residues
MGYMEGIMSKITIKEVAKRANVSTATVSRVLNNNYPVSDEARKEVLKAASELNYKPNGVARSLKKNKTNLIGIIVADISN
PFFMSVARGVESVVSEEYNLILSSTDESPDKEVKLLNMMLEKKVDGIVIASSNIDSSAIKSIIASGIKLVLIDRKVDDVT
LDYIANDNFNGSFLLTESLIKKGHKDIAIVNGCLNVTTAIERFNGYRAAMNKYGLKIDKDFILQGNYSASIAFVETRNLI
RKGKLPTALLSANNSMTEGIMKALKSEKVRIPEDISLVSFGEITNQEFIEPKITCIKQNPFLMGQKAGEVILDKLNGKSK
EDNFKEVVLINEFCEGNSIKSLI
>Mature_342_residues
GYMEGIMSKITIKEVAKRANVSTATVSRVLNNNYPVSDEARKEVLKAASELNYKPNGVARSLKKNKTNLIGIIVADISNP
FFMSVARGVESVVSEEYNLILSSTDESPDKEVKLLNMMLEKKVDGIVIASSNIDSSAIKSIIASGIKLVLIDRKVDDVTL
DYIANDNFNGSFLLTESLIKKGHKDIAIVNGCLNVTTAIERFNGYRAAMNKYGLKIDKDFILQGNYSASIAFVETRNLIR
KGKLPTALLSANNSMTEGIMKALKSEKVRIPEDISLVSFGEITNQEFIEPKITCIKQNPFLMGQKAGEVILDKLNGKSKE
DNFKEVVLINEFCEGNSIKSLI

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=308, Percent_Identity=32.7922077922078, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787948, Length=320, Percent_Identity=31.25, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=28.4789644012945, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=28.8401253918495, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789202, Length=287, Percent_Identity=29.616724738676, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789068, Length=290, Percent_Identity=30, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788474, Length=309, Percent_Identity=28.1553398058252, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=28.5266457680251, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=22.2222222222222, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=26.984126984127, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787906, Length=259, Percent_Identity=30.5019305019305, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790715, Length=336, Percent_Identity=22.0238095238095, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789456, Length=321, Percent_Identity=26.1682242990654, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790689, Length=322, Percent_Identity=23.6024844720497, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37772; Mature: 37641

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYMEGIMSKITIKEVAKRANVSTATVSRVLNNNYPVSDEARKEVLKAASELNYKPNGVA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
RSLKKNKTNLIGIIVADISNPFFMSVARGVESVVSEEYNLILSSTDESPDKEVKLLNMML
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHH
EKKVDGIVIASSNIDSSAIKSIIASGIKLVLIDRKVDDVTLDYIANDNFNGSFLLTESLI
HHCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCEEEHHHHH
KKGHKDIAIVNGCLNVTTAIERFNGYRAAMNKYGLKIDKDFILQGNYSASIAFVETRNLI
HCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHCCCEECCEEEEECCCCEEEEEEEHHHHH
RKGKLPTALLSANNSMTEGIMKALKSEKVRIPEDISLVSFGEITNQEFIEPKITCIKQNP
HHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHCCCEEEEEECCC
FLMGQKAGEVILDKLNGKSKEDNFKEVVLINEFCEGNSIKSLI
EEECCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHCC
>Mature Secondary Structure 
GYMEGIMSKITIKEVAKRANVSTATVSRVLNNNYPVSDEARKEVLKAASELNYKPNGVA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
RSLKKNKTNLIGIIVADISNPFFMSVARGVESVVSEEYNLILSSTDESPDKEVKLLNMML
HHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHH
EKKVDGIVIASSNIDSSAIKSIIASGIKLVLIDRKVDDVTLDYIANDNFNGSFLLTESLI
HHCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCEEEHHHHH
KKGHKDIAIVNGCLNVTTAIERFNGYRAAMNKYGLKIDKDFILQGNYSASIAFVETRNLI
HCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHCCCEECCEEEEECCCCEEEEEEEHHHHH
RKGKLPTALLSANNSMTEGIMKALKSEKVRIPEDISLVSFGEITNQEFIEPKITCIKQNP
HHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHCCCEEEEEECCC
FLMGQKAGEVILDKLNGKSKEDNFKEVVLINEFCEGNSIKSLI
EEECCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA