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The map label for this gene is vanS [H]

Identifier: 28210523

GI number: 28210523

Start: 850345

End: 851910

Strand: Direct

Name: vanS [H]

Synonym: CTC00806

Alternate gene names: 28210523

Gene position: 850345-851910 (Clockwise)

Preceding gene: 28210522

Following gene: 28210524

Centisome position: 30.38

GC content: 25.67

Gene sequence:

>1566_bases
ATGATAAGAAAATTGATAAAATTTATATGGAGAGTTATTACCAAACCTTTTAGATATATATTTGGTAAAATAGGTATTAG
ATTAAAAAGATTATATAATAAATTATATATAATCTATGAAAATTTAATGGAAAAAATAAATAAAAATATAAGATTTCAAT
TAATAGTAACTTTTGGAGTGTGTCTATTTCTTTCATTTATACTTTTTACTATGTTAAGTTCATATTTTTCTAGTAAAAAT
GTTCAAACTACTATAGATTATAATGAGGATATAAAAAGAATATCTGAAAATGCTTTCAGAATTGGAGAAACTTTAGATAA
AGGTAAATTGTCTTCAAAAGATGATGAAAAAATAAATCAATTAATAGATCAAAATTATGGAGAAAGATATAAAATATTAA
TAACAGACTTAAAAGGAAATATTATTTATAAAACCAAGAATACTTATGAAACCCAAGTTGATATATATACAGTTATAAAA
AATGCTATGGCAATTGGAGAAAGAAATAGATATGAAGAATTAAATACCAACAAAGAAGTTGTGACTTTTTATCCTATAGA
ATTTACAGATGTAAGAGGATATGTAATAGTCAAAGCTTACCCAGAAGGAATAGTAAAAACCCATAGATATACTGATGGTG
GAAAACCTTTTTTATCATTGGTAATATCAATAATGTTCTTTTTAGGGCTATTTTTTTATTTAACTGGTAAGAAGGTTCAC
TATATACAAGAAATTGCTGATGCCGTTAAAGAAGTTGCTAATGGGAATTTGAAATATAGGGTACAAAGAAAGGGAAATGA
TGAGATTTCCAATTTAGCAGACAATATAAATAATATGACAAATGAATTAAACAAGAGAATAGAAAAGGAAAGAGAAATAG
AAAAGACGAAAAGTGAACTTATTACTAATGTGTCTCATGATCTAAGAACACCTTTAACATCAATACTTGGGTATTTAAAC
TTAATAAAGGATGGAAAATATAGTGGCGAAGAAGAAATGTTAGAGTTTATACAAATAGCTTACAATAAATCTGAAAAACT
TAGAGTGTTGATTGATGAATTATTTGAATATACAAGACTATCTAGTGATGGGATTAAAATAAGTAAAGTAGAAATAGCAC
TAGATGAACTTATAGAACAACTTGTGGAAGAATTTATACCTGTATTTTTAGAAAATAAAATAGAGATTAAAAGAAATATA
GAATCTAACATTAAGATAGATGCAGATGGAGATAAATTGGTAAGGGTTTTTGAGAATTTATTTATGAATGCAGTAAGGTA
TAGTTATAAACCAGGATTAATAAAAATAAACTTGATTAATGAATATGGATATGCTAAATTTTCTATAACCAATAAGGGGA
AAAGTTTAAGAAAAGATGAGGTAGACAAATTATTTGATAGATTTTATAGAGTAGAAAAATCTAGATCTGAAACCACAGGA
GGAACTGGCTTAGGTCTTGCCATATCTAAAAGTATAGTTTCACTTCATCATGGAGAAATATGGGGCAGTTGCCAAGGGGA
AGAAATAACATTTTATGTTAAATTAAAAATAAGTGAAGGCAATTAA

Upstream 100 bases:

>100_bases
AGAGAAAAGATAGAGGAAAATCCTAGAAAACCTAAGTATGTAAAGACTGTATGGGGTGTAGGCTATAAGGTAGAAAAGTT
ATAAAATTAAAGAGGTAAAT

Downstream 100 bases:

>100_bases
AATAAAGTAATGTAGGGGGATGATTAAAATAGAACAAATTAATTTAGAAAAAAGTAAAGGAAGAGCAGTGGCATTTATGC
TATTATCCTCTTTTTGTTTT

Product: sensory transduction protein kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MIRKLIKFIWRVITKPFRYIFGKIGIRLKRLYNKLYIIYENLMEKINKNIRFQLIVTFGVCLFLSFILFTMLSSYFSSKN
VQTTIDYNEDIKRISENAFRIGETLDKGKLSSKDDEKINQLIDQNYGERYKILITDLKGNIIYKTKNTYETQVDIYTVIK
NAMAIGERNRYEELNTNKEVVTFYPIEFTDVRGYVIVKAYPEGIVKTHRYTDGGKPFLSLVISIMFFLGLFFYLTGKKVH
YIQEIADAVKEVANGNLKYRVQRKGNDEISNLADNINNMTNELNKRIEKEREIEKTKSELITNVSHDLRTPLTSILGYLN
LIKDGKYSGEEEMLEFIQIAYNKSEKLRVLIDELFEYTRLSSDGIKISKVEIALDELIEQLVEEFIPVFLENKIEIKRNI
ESNIKIDADGDKLVRVFENLFMNAVRYSYKPGLIKINLINEYGYAKFSITNKGKSLRKDEVDKLFDRFYRVEKSRSETTG
GTGLGLAISKSIVSLHHGEIWGSCQGEEITFYVKLKISEGN

Sequences:

>Translated_521_residues
MIRKLIKFIWRVITKPFRYIFGKIGIRLKRLYNKLYIIYENLMEKINKNIRFQLIVTFGVCLFLSFILFTMLSSYFSSKN
VQTTIDYNEDIKRISENAFRIGETLDKGKLSSKDDEKINQLIDQNYGERYKILITDLKGNIIYKTKNTYETQVDIYTVIK
NAMAIGERNRYEELNTNKEVVTFYPIEFTDVRGYVIVKAYPEGIVKTHRYTDGGKPFLSLVISIMFFLGLFFYLTGKKVH
YIQEIADAVKEVANGNLKYRVQRKGNDEISNLADNINNMTNELNKRIEKEREIEKTKSELITNVSHDLRTPLTSILGYLN
LIKDGKYSGEEEMLEFIQIAYNKSEKLRVLIDELFEYTRLSSDGIKISKVEIALDELIEQLVEEFIPVFLENKIEIKRNI
ESNIKIDADGDKLVRVFENLFMNAVRYSYKPGLIKINLINEYGYAKFSITNKGKSLRKDEVDKLFDRFYRVEKSRSETTG
GTGLGLAISKSIVSLHHGEIWGSCQGEEITFYVKLKISEGN
>Mature_521_residues
MIRKLIKFIWRVITKPFRYIFGKIGIRLKRLYNKLYIIYENLMEKINKNIRFQLIVTFGVCLFLSFILFTMLSSYFSSKN
VQTTIDYNEDIKRISENAFRIGETLDKGKLSSKDDEKINQLIDQNYGERYKILITDLKGNIIYKTKNTYETQVDIYTVIK
NAMAIGERNRYEELNTNKEVVTFYPIEFTDVRGYVIVKAYPEGIVKTHRYTDGGKPFLSLVISIMFFLGLFFYLTGKKVH
YIQEIADAVKEVANGNLKYRVQRKGNDEISNLADNINNMTNELNKRIEKEREIEKTKSELITNVSHDLRTPLTSILGYLN
LIKDGKYSGEEEMLEFIQIAYNKSEKLRVLIDELFEYTRLSSDGIKISKVEIALDELIEQLVEEFIPVFLENKIEIKRNI
ESNIKIDADGDKLVRVFENLFMNAVRYSYKPGLIKINLINEYGYAKFSITNKGKSLRKDEVDKLFDRFYRVEKSRSETTG
GTGLGLAISKSIVSLHHGEIWGSCQGEEITFYVKLKISEGN

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=27.7992277992278, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=26.3392857142857, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=28.2511210762332, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788713, Length=263, Percent_Identity=25.0950570342205, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=27.5109170305677, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=291, Percent_Identity=24.7422680412371, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788549, Length=253, Percent_Identity=25.6916996047431, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=270, Percent_Identity=25.1851851851852, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=339, Percent_Identity=23.598820058997, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082128, Length=236, Percent_Identity=25, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=306, Percent_Identity=24.8366013071895, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=232, Percent_Identity=28.0172413793103, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=23.6607142857143, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789808, Length=289, Percent_Identity=24.2214532871972, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790861, Length=384, Percent_Identity=18.75, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1789403, Length=285, Percent_Identity=21.7543859649123, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790551, Length=292, Percent_Identity=23.972602739726, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60648; Mature: 60648

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKLIKFIWRVITKPFRYIFGKIGIRLKRLYNKLYIIYENLMEKINKNIRFQLIVTFGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
CLFLSFILFTMLSSYFSSKNVQTTIDYNEDIKRISENAFRIGETLDKGKLSSKDDEKINQ
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCHHCCCCCCCCCHHHHHH
LIDQNYGERYKILITDLKGNIIYKTKNTYETQVDIYTVIKNAMAIGERNRYEELNTNKEV
HHHCCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCE
VTFYPIEFTDVRGYVIVKAYPEGIVKTHRYTDGGKPFLSLVISIMFFLGLFFYLTGKKVH
EEEEEEEEECCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
YIQEIADAVKEVANGNLKYRVQRKGNDEISNLADNINNMTNELNKRIEKEREIEKTKSEL
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITNVSHDLRTPLTSILGYLNLIKDGKYSGEEEMLEFIQIAYNKSEKLRVLIDELFEYTRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
SSDGIKISKVEIALDELIEQLVEEFIPVFLENKIEIKRNIESNIKIDADGDKLVRVFENL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEEECCHHHHHHHHHHH
FMNAVRYSYKPGLIKINLINEYGYAKFSITNKGKSLRKDEVDKLFDRFYRVEKSRSETTG
HHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GTGLGLAISKSIVSLHHGEIWGSCQGEEITFYVKLKISEGN
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCC
>Mature Secondary Structure
MIRKLIKFIWRVITKPFRYIFGKIGIRLKRLYNKLYIIYENLMEKINKNIRFQLIVTFGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
CLFLSFILFTMLSSYFSSKNVQTTIDYNEDIKRISENAFRIGETLDKGKLSSKDDEKINQ
HHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCHHCCCCCCCCCHHHHHH
LIDQNYGERYKILITDLKGNIIYKTKNTYETQVDIYTVIKNAMAIGERNRYEELNTNKEV
HHHCCCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCE
VTFYPIEFTDVRGYVIVKAYPEGIVKTHRYTDGGKPFLSLVISIMFFLGLFFYLTGKKVH
EEEEEEEEECCCCEEEEEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
YIQEIADAVKEVANGNLKYRVQRKGNDEISNLADNINNMTNELNKRIEKEREIEKTKSEL
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITNVSHDLRTPLTSILGYLNLIKDGKYSGEEEMLEFIQIAYNKSEKLRVLIDELFEYTRL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
SSDGIKISKVEIALDELIEQLVEEFIPVFLENKIEIKRNIESNIKIDADGDKLVRVFENL
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCEEEECCHHHHHHHHHHH
FMNAVRYSYKPGLIKINLINEYGYAKFSITNKGKSLRKDEVDKLFDRFYRVEKSRSETTG
HHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GTGLGLAISKSIVSLHHGEIWGSCQGEEITFYVKLKISEGN
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]