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The map label for this gene is trmB

Identifier: 28210511

GI number: 28210511

Start: 840586

End: 841221

Strand: Direct

Name: trmB

Synonym: CTC00794

Alternate gene names: 28210511

Gene position: 840586-841221 (Clockwise)

Preceding gene: 28210510

Following gene: 28210512

Centisome position: 30.03

GC content: 25.79

Gene sequence:

>636_bases
ATGCGACTTAGAAAAAAGTGGTGGGCTAGACCAGAAATGGAGGCTTCATCTTTAGTAATAACAAATCCAAAAGAATATAA
AGGAAAATGGATGGAAGAATTTAAAAACAATAATAGTATTCATTTAGAATTAGGATGTGGAAGAGGAGGATTTATGCAAG
AAAAAGCTCTTAAAAATCCTAATATAAACTATGTGGCGGTAGACTTAAAAGATGAAATTTTAATTTATGTATTAAGAAAA
CTGAAAGAAAAAGAAATAGAAAATGTAAGGATTGTACCTTTAAATATAGCTTTTATAAGTGAAGTATTTGAAAAGGATGA
GATAGAAAAGATATATATAAATTTTTGTAACCCATGGCCTAAATTAAGACATAATAAAAGAAGACTTACTCATAATAAAT
TTTTAGATGAATATAAAAAATTTTTGAAGGGTCAAGGTGAAATATGGTTTAAAACAGATGACATAGGATTGTTTGAAGAT
TCCCAAGAATATTTTAAAGAAAGTGGATTTTCCATAGAATATATAACCTATGATTTACATAAATCAGATTTTAAAGACAA
TATAATGACAGAATATGAAACTAAGTTTACATCTATGGGAATGAAAACTATGTTTTTAATAGCAAAATTAAAATAA

Upstream 100 bases:

>100_bases
TGGAAACCTGCTAACGTGACCTATGGATTAATACTTAGACTATTCAATATTATCTAGTAGGAAATAGCTAATAGTCATTC
ACTAAGTAGAGGAGGATTTT

Downstream 100 bases:

>100_bases
CCAATAAATAAAGTTTTGAACCCCGAAATCCATATTATAGCTAAAGTGCAGCTTCCAGATATGGATTTTTGGGAGTAATA
TCAGCAAAATAGATATATAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MRLRKKWWARPEMEASSLVITNPKEYKGKWMEEFKNNNSIHLELGCGRGGFMQEKALKNPNINYVAVDLKDEILIYVLRK
LKEKEIENVRIVPLNIAFISEVFEKDEIEKIYINFCNPWPKLRHNKRRLTHNKFLDEYKKFLKGQGEIWFKTDDIGLFED
SQEYFKESGFSIEYITYDLHKSDFKDNIMTEYETKFTSMGMKTMFLIAKLK

Sequences:

>Translated_211_residues
MRLRKKWWARPEMEASSLVITNPKEYKGKWMEEFKNNNSIHLELGCGRGGFMQEKALKNPNINYVAVDLKDEILIYVLRK
LKEKEIENVRIVPLNIAFISEVFEKDEIEKIYINFCNPWPKLRHNKRRLTHNKFLDEYKKFLKGQGEIWFKTDDIGLFED
SQEYFKESGFSIEYITYDLHKSDFKDNIMTEYETKFTSMGMKTMFLIAKLK
>Mature_211_residues
MRLRKKWWARPEMEASSLVITNPKEYKGKWMEEFKNNNSIHLELGCGRGGFMQEKALKNPNINYVAVDLKDEILIYVLRK
LKEKEIENVRIVPLNIAFISEVFEKDEIEKIYINFCNPWPKLRHNKRRLTHNKFLDEYKKFLKGQGEIWFKTDDIGLFED
SQEYFKESGFSIEYITYDLHKSDFKDNIMTEYETKFTSMGMKTMFLIAKLK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CLOTE (Q897E6)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781455.1
- ProteinModelPortal:   Q897E6
- SMR:   Q897E6
- GeneID:   1059790
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00794
- NMPDR:   fig|212717.1.peg.677
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- BioCyc:   CTET212717:CTC_00794-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25320; Mature: 25320

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: BINDING 43-43 BINDING 68-68 BINDING 95-95 BINDING 117-117 BINDING 121-121 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRKKWWARPEMEASSLVITNPKEYKGKWMEEFKNNNSIHLELGCGRGGFMQEKALKNP
CCCCCHHCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCC
NINYVAVDLKDEILIYVLRKLKEKEIENVRIVPLNIAFISEVFEKDEIEKIYINFCNPWP
CCCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCEEEEEEECCCCH
KLRHNKRRLTHNKFLDEYKKFLKGQGEIWFKTDDIGLFEDSQEYFKESGFSIEYITYDLH
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEEEEEECC
KSDFKDNIMTEYETKFTSMGMKTMFLIAKLK
CCCCHHHHHHHHHHHHHHCCHHEEEEEEECC
>Mature Secondary Structure
MRLRKKWWARPEMEASSLVITNPKEYKGKWMEEFKNNNSIHLELGCGRGGFMQEKALKNP
CCCCCHHCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCC
NINYVAVDLKDEILIYVLRKLKEKEIENVRIVPLNIAFISEVFEKDEIEKIYINFCNPWP
CCCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCEEEEEEECCCCH
KLRHNKRRLTHNKFLDEYKKFLKGQGEIWFKTDDIGLFEDSQEYFKESGFSIEYITYDLH
HHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEEEEEECC
KSDFKDNIMTEYETKFTSMGMKTMFLIAKLK
CCCCHHHHHHHHHHHHHHCCHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129