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The map label for this gene is pncA [H]

Identifier: 28210422

GI number: 28210422

Start: 722086

End: 722661

Strand: Direct

Name: pncA [H]

Synonym: CTC00699

Alternate gene names: 28210422

Gene position: 722086-722661 (Clockwise)

Preceding gene: 28210421

Following gene: 28210425

Centisome position: 25.8

GC content: 27.95

Gene sequence:

>576_bases
ATGGAGGTTGATAATTTGAGTAAGGCTATAATAGTGGTAGATTGTCAAAATGATTTTATAAGTGGAAGCTTAGCTTGTCA
TATGGGTAAAGAATCTGTAGATAATATAATAAGTTTAATTAATAGCAATAAAGATTTTAAGGTGTTTTATTCCTGTGATT
GGCATAGTAAAAATAATAAATCATTTAAAGCAAATGGCGGTATATGGCCTATTCATTGTGTGGGATATGAGGAAGGGGCA
GAATTGCATCAAAACTTTTATAAAAATATAGAAAATGAAAAACAGAGTCCATTGAATAAAGAAAATATTTTTTATAAGGG
ACAGGATGATGAAATAGAAGAGTATTCTGCTTTTTATGCTAAAAATTCTAAAGGAGAAATCCTCAACAACAAAATTCCCA
AGGAAGTAATAGTTTGTGGAATAGCATCTGAATACTGCGTAAGAGAGACTATAAAGGAATTTTTAGAATATGATTTCACG
GTTAGTTTATTTTTAGATGGATTGGGATATGTAAATAAAGAAGAGCATAATAAAAATATAGAAGAGTTAAAAAAATTAGA
TGTAAATATAATTTAG

Upstream 100 bases:

>100_bases
TTCCTATGTACACATTTACAGATTTTATTAGTGAGTAGATTCATTTAAAATGTATATACAGATGTATATAGGAAGTGTTA
TAATATATTAAAATGTAGGG

Downstream 100 bases:

>100_bases
ATATTTAAATTATACAATAAAATGCACAGTTTAAAATTTTTAAACTGTGCATTTTTTATATTAAGAAAACCCCCAGCTAA
GCTAAGGTCTGTTTATAACA

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEVDNLSKAIIVVDCQNDFISGSLACHMGKESVDNIISLINSNKDFKVFYSCDWHSKNNKSFKANGGIWPIHCVGYEEGA
ELHQNFYKNIENEKQSPLNKENIFYKGQDDEIEEYSAFYAKNSKGEILNNKIPKEVIVCGIASEYCVRETIKEFLEYDFT
VSLFLDGLGYVNKEEHNKNIEELKKLDVNII

Sequences:

>Translated_191_residues
MEVDNLSKAIIVVDCQNDFISGSLACHMGKESVDNIISLINSNKDFKVFYSCDWHSKNNKSFKANGGIWPIHCVGYEEGA
ELHQNFYKNIENEKQSPLNKENIFYKGQDDEIEEYSAFYAKNSKGEILNNKIPKEVIVCGIASEYCVRETIKEFLEYDFT
VSLFLDGLGYVNKEEHNKNIEELKKLDVNII
>Mature_191_residues
MEVDNLSKAIIVVDCQNDFISGSLACHMGKESVDNIISLINSNKDFKVFYSCDWHSKNNKSFKANGGIWPIHCVGYEEGA
ELHQNFYKNIENEKQSPLNKENIFYKGQDDEIEEYSAFYAKNSKGEILNNKIPKEVIVCGIASEYCVRETIKEFLEYDFT
VSLFLDGLGYVNKEEHNKNIEELKKLDVNII

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=30.2564102564103, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=219, Percent_Identity=28.310502283105, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21925; Mature: 21925

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVDNLSKAIIVVDCQNDFISGSLACHMGKESVDNIISLINSNKDFKVFYSCDWHSKNNK
CCCCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
SFKANGGIWPIHCVGYEEGAELHQNFYKNIENEKQSPLNKENIFYKGQDDEIEEYSAFYA
CEEECCCEEEEEEECCCCHHHHHHHHHHHHCCHHCCCCCCCCEEECCCCCCHHHHHHHHH
KNSKGEILNNKIPKEVIVCGIASEYCVRETIKEFLEYDFTVSLFLDGLGYVNKEEHNKNI
CCCCCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHCCCHHHHEEHHCCCCCCHHCCCCH
EELKKLDVNII
HHHHHCCCCCC
>Mature Secondary Structure
MEVDNLSKAIIVVDCQNDFISGSLACHMGKESVDNIISLINSNKDFKVFYSCDWHSKNNK
CCCCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
SFKANGGIWPIHCVGYEEGAELHQNFYKNIENEKQSPLNKENIFYKGQDDEIEEYSAFYA
CEEECCCEEEEEEECCCCHHHHHHHHHHHHCCHHCCCCCCCCEEECCCCCCHHHHHHHHH
KNSKGEILNNKIPKEVIVCGIASEYCVRETIKEFLEYDFTVSLFLDGLGYVNKEEHNKNI
CCCCCCCCCCCCCHHHEEECCHHHHHHHHHHHHHHHCCCHHHHEEHHCCCCCCHHCCCCH
EELKKLDVNII
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]