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The map label for this gene is yflA [H]

Identifier: 28210301

GI number: 28210301

Start: 583153

End: 584580

Strand: Direct

Name: yflA [H]

Synonym: CTC00564

Alternate gene names: 28210301

Gene position: 583153-584580 (Clockwise)

Preceding gene: 28210300

Following gene: 28210302

Centisome position: 20.83

GC content: 32.21

Gene sequence:

>1428_bases
ATGGATTTATCTAATCAAGTTACAGAGACATATGCAATCCTATATTTAAATAAAAAGTTCAGGAGGAATGTTTTAATGCA
AACGATTCAAATGATAACAGACTTTTTATGGGGATGGCCATTAATAATATTAATTGTTGCAGTATCCCTAATGTACAGTA
TCGATCTTAAGTTCTTACAATTCAGAAAATTTGGATTTATAATGAAGAATACTTTTGGAAAAATGTTTGAAAAAGGCACT
TCAGGAGAAGGAACAGTAACACCATTCCAAGCAGTATCAACAGCTTTGTCTGCAACTCTAGGAGTTGGTAGTATAGCAGG
AGTAGGTGTAGCCCTTGCAATAGGTGGTGCAGGAGCTATATTTTGGATGTGGATAGTTGCCTTTGTAGCAATGATCACAA
AATATGCTGAAATAACTTTAGCAGTAAAATACCGCGAGAAGAATCCAGAAACTGGAGAATTCCAAGGTGGACCTATGTAT
TACATAAAAAATGGTTTAGGCGAAAAATATAATTGGTTATCTGTATTTTGGTCTATAATATTTATGATAATGATGTTCAT
AGGAGGATTTGTACAATCTAACTCAGCAGCAGAGGTTGTATATGAAACATTTAAAGTAGATAGAGTAGTAGTTGGAATTA
TATTTGCTGTAATAACAGCAATAGTAATTTTAGGTGGAATAAAAACTATAGGAAAATTTGCAGAAAAAGTAATACCAACA
ATGACACTTTTATATGTTGTAGCAGGATTAATAGTTATATTTTTAAATGCAAATCAAATAATACCATCTTTTCAGAAAAT
ATTTACAGATGCATTTAAAGGTAGTGCAGCGGTAGGAGGATTTGCAGGAGCAACTATACTTGCAGCTATGAGAAATGGAT
TTGCAAGAGGAGTTTATTCAAATGAATCAGGTATGGGAACAGCACCAGTAGCCCATTCATCAGCTACAACTTCCTATGCA
GCAAAGCAAGGATTATGGGGTATATTTGAAGTTTTTGTAACATCAATTGTAGTTTGTTCAATGACAGCCCTTATTATATT
AACTAGTGGAGTGCTAGAAACTGGTAAAGAAGGTGCAGTTTTAACTGCTACAGCTTTTGGAAGTTCACTAGGATTTACTG
GTAAGTTAATTGTAACTCTTTGCGTAGTTTTATTTGCATTCACCACTGTATTAGTAGATGGATACTATTCAGAAACAGGA
CTTTTATATGCTACAAAAACTAACAAGTTATTAAAAGTATTTAGAATATTATATTGTTTAGGTTTAATATACGGATCTAT
AGGTGGATTAAAGGTTGTCTGGGGATTATTTGATTTCTTCATGGCATTTGAAGTAATAGTAAACCTAATAGTTCTTGTAA
AATTAAGAAAAGTAGTAGTAGAAGAAACAGAATTATACTTTAATAGTGATGAAGTAAAAAAAATGTAG

Upstream 100 bases:

>100_bases
TATTGCTGAAATTAGTATAATAGTGATACTTTCAATATGTGTACACATGGATATTTAAATAAATACGTGATAAGTATGTT
AAAAATTTAATAATATAAAG

Downstream 100 bases:

>100_bases
GTTTATACTAAGAGAATTAAAAAAACAAAAATAGAATGAAAAATGAATTTTAAATTAATAAAGTGGTCATTATCTAAAAT
ATTAGATAGTGACTATTTTG

Product: Na+-linked D-alanine/glycine permease

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MDLSNQVTETYAILYLNKKFRRNVLMQTIQMITDFLWGWPLIILIVAVSLMYSIDLKFLQFRKFGFIMKNTFGKMFEKGT
SGEGTVTPFQAVSTALSATLGVGSIAGVGVALAIGGAGAIFWMWIVAFVAMITKYAEITLAVKYREKNPETGEFQGGPMY
YIKNGLGEKYNWLSVFWSIIFMIMMFIGGFVQSNSAAEVVYETFKVDRVVVGIIFAVITAIVILGGIKTIGKFAEKVIPT
MTLLYVVAGLIVIFLNANQIIPSFQKIFTDAFKGSAAVGGFAGATILAAMRNGFARGVYSNESGMGTAPVAHSSATTSYA
AKQGLWGIFEVFVTSIVVCSMTALIILTSGVLETGKEGAVLTATAFGSSLGFTGKLIVTLCVVLFAFTTVLVDGYYSETG
LLYATKTNKLLKVFRILYCLGLIYGSIGGLKVVWGLFDFFMAFEVIVNLIVLVKLRKVVVEETELYFNSDEVKKM

Sequences:

>Translated_475_residues
MDLSNQVTETYAILYLNKKFRRNVLMQTIQMITDFLWGWPLIILIVAVSLMYSIDLKFLQFRKFGFIMKNTFGKMFEKGT
SGEGTVTPFQAVSTALSATLGVGSIAGVGVALAIGGAGAIFWMWIVAFVAMITKYAEITLAVKYREKNPETGEFQGGPMY
YIKNGLGEKYNWLSVFWSIIFMIMMFIGGFVQSNSAAEVVYETFKVDRVVVGIIFAVITAIVILGGIKTIGKFAEKVIPT
MTLLYVVAGLIVIFLNANQIIPSFQKIFTDAFKGSAAVGGFAGATILAAMRNGFARGVYSNESGMGTAPVAHSSATTSYA
AKQGLWGIFEVFVTSIVVCSMTALIILTSGVLETGKEGAVLTATAFGSSLGFTGKLIVTLCVVLFAFTTVLVDGYYSETG
LLYATKTNKLLKVFRILYCLGLIYGSIGGLKVVWGLFDFFMAFEVIVNLIVLVKLRKVVVEETELYFNSDEVKKM
>Mature_475_residues
MDLSNQVTETYAILYLNKKFRRNVLMQTIQMITDFLWGWPLIILIVAVSLMYSIDLKFLQFRKFGFIMKNTFGKMFEKGT
SGEGTVTPFQAVSTALSATLGVGSIAGVGVALAIGGAGAIFWMWIVAFVAMITKYAEITLAVKYREKNPETGEFQGGPMY
YIKNGLGEKYNWLSVFWSIIFMIMMFIGGFVQSNSAAEVVYETFKVDRVVVGIIFAVITAIVILGGIKTIGKFAEKVIPT
MTLLYVVAGLIVIFLNANQIIPSFQKIFTDAFKGSAAVGGFAGATILAAMRNGFARGVYSNESGMGTAPVAHSSATTSYA
AKQGLWGIFEVFVTSIVVCSMTALIILTSGVLETGKEGAVLTATAFGSSLGFTGKLIVTLCVVLFAFTTVLVDGYYSETG
LLYATKTNKLLKVFRILYCLGLIYGSIGGLKVVWGLFDFFMAFEVIVNLIVLVKLRKVVVEETELYFNSDEVKKM

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=452, Percent_Identity=32.7433628318584, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51798; Mature: 51798

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSNQVTETYAILYLNKKFRRNVLMQTIQMITDFLWGWPLIILIVAVSLMYSIDLKFLQ
CCCCHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRKFGFIMKNTFGKMFEKGTSGEGTVTPFQAVSTALSATLGVGSIAGVGVALAIGGAGAI
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHH
FWMWIVAFVAMITKYAEITLAVKYREKNPETGEFQGGPMYYIKNGLGEKYNWLSVFWSII
HHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
FMIMMFIGGFVQSNSAAEVVYETFKVDRVVVGIIFAVITAIVILGGIKTIGKFAEKVIPT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTLLYVVAGLIVIFLNANQIIPSFQKIFTDAFKGSAAVGGFAGATILAAMRNGFARGVYS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
NESGMGTAPVAHSSATTSYAAKQGLWGIFEVFVTSIVVCSMTALIILTSGVLETGKEGAV
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LTATAFGSSLGFTGKLIVTLCVVLFAFTTVLVDGYYSETGLLYATKTNKLLKVFRILYCL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHH
GLIYGSIGGLKVVWGLFDFFMAFEVIVNLIVLVKLRKVVVEETELYFNSDEVKKM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCC
>Mature Secondary Structure
MDLSNQVTETYAILYLNKKFRRNVLMQTIQMITDFLWGWPLIILIVAVSLMYSIDLKFLQ
CCCCHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRKFGFIMKNTFGKMFEKGTSGEGTVTPFQAVSTALSATLGVGSIAGVGVALAIGGAGAI
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHH
FWMWIVAFVAMITKYAEITLAVKYREKNPETGEFQGGPMYYIKNGLGEKYNWLSVFWSII
HHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
FMIMMFIGGFVQSNSAAEVVYETFKVDRVVVGIIFAVITAIVILGGIKTIGKFAEKVIPT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTLLYVVAGLIVIFLNANQIIPSFQKIFTDAFKGSAAVGGFAGATILAAMRNGFARGVYS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
NESGMGTAPVAHSSATTSYAAKQGLWGIFEVFVTSIVVCSMTALIILTSGVLETGKEGAV
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LTATAFGSSLGFTGKLIVTLCVVLFAFTTVLVDGYYSETGLLYATKTNKLLKVFRILYCL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHH
GLIYGSIGGLKVVWGLFDFFMAFEVIVNLIVLVKLRKVVVEETELYFNSDEVKKM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]