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The map label for this gene is clpB

Identifier: 28210275

GI number: 28210275

Start: 549604

End: 552201

Strand: Direct

Name: clpB

Synonym: CTC00536

Alternate gene names: 28210275

Gene position: 549604-552201 (Clockwise)

Preceding gene: 28210274

Following gene: 28210276

Centisome position: 19.63

GC content: 28.98

Gene sequence:

>2598_bases
ATGGATATAGAAAAATTAACACTAAAAGTTCAGCAAACAATAAATGATTCTCAAAAAATTGCAGTAAAGTATAATCATCA
GCAATTAGAGCCATTTCATCTTTTTGCAGCTTTAGTTTTTCAAGAAGACGGATTAATCCCAAATATTTTAGGGAAAATGA
ATATAAATATAAAAGTTTTAAGAGATGAAATAAAAAGAGAATTAAATGAAATGCCTAAGGTATTAGGAGATGGAGCGCAA
AATAGTGGAGTATATGCTACTAGATCCTTTGAAGAAATTTTTATTAGAGCAGAAAGTATAGCTAAGGATTTTAAGGATTC
ATATATTAGCGTGGAACATATAATGCTTTCTCTAATGCAAGGAAGGTCTACTTCTATTAAAAAGATATTAGATAAATTTA
ATATAAGAAAAGATAAGTTTTTAAATGTATTACAGCAGGTGAGAGGAAACCAAAGAGTTGATACTCAGGATCCTGAAGGA
ACTTATGAGGCTTTAGTAAAATACGGAAGAAACTTAATAGAAGATGCTAAAAAACATAAATTAGATCCTGTTATAGGTAG
AGATGAAGAAATAAGAAGAATTGTAAGAATTCTTTCAAGAAGAACTAAAAATAATCCAGTATTAATAGGAGATCCGGGAG
TTGGTAAGACAGCTATTATTGAAGGACTTGCTGAAAGAATAGTAAGAGGAGATGTACCAGAAGGGCTAAAGAATAAAATT
ATATTTTCCTTAGATATGGGTGCTTTAATTGCAGGAGCTAAATTCAGAGGAGAATTTGAAGAGAGATTAAAAGCTGTACT
TAAGGAAGTCGAAAATAGCCAAGGAAAAATTATATTATTCATAGACGAAATACACAATATAGTTGGAGCAGGAAAAACTG
AAGGATCTATGGATGCAGGTAATTTAATAAAACCAATGTTAGCTAGAGGAGAGCTAAATTGTATTGGAGCTACTACCTTT
GATGAATATAGAAAATATATAGAAAAAGATAAGGCATTAGAAAGAAGATTTCAACCAGTAATTATAGATGAACCAACAGT
AGAGGATACTATATCAATAATAAGAGGTCTTAAGGAAAGATTTGAAATACATCATGGAATAAGAATTCATGATTCAGCTA
TCGTGGCAGCAGCAAAATTATCTCAAAGATATATAACAGACAGATATCTTCCGGATAAGGCTATTGACCTAATAGATGAA
GCAGGGGCTATGATAAGAACTGAAATAGATAGCTTACCAACAGAACTAGATTCTATAAAGAGGAAAATATTTCAAATGGA
GATAGAAAAAGAAGCTCTAGCTAAAGAAAAAGATAGTAGATCAAAAGAAAGATTGGAAGATTTAGAAAAAGAATTAAGCA
ATTTAAAAGAAAAAGATAAAGAGATGACTGCAAAATATGAAAAAGAAAAAGAACAAATAATTAATATGAGAAATTTAAAG
CAAAAGCTAGATGAAGTTAAGGGACAGTTAGAAAAAGCAGAAAGAGAGTATGATCTAAATAAAGTAGCAGAATTAAAATA
TGGTATAATACCAGGGATTAAAAGTCAAATAGAGGAAAAAGAAATTCTTATAAAAGAAAATAGTCAAGGGAATATGCTAA
AAGAAGAAGTAACAGAGAATGAGATATCTAAAATAATATCTCATTGGACAGGAATACCAGTAACAAAACTTATTGAAGGA
GAGAAAGACAAATTATTAAGATTAGAAGATGAACTTAAGAGTAGAGTTATAGGTCAAGATGAAGCAGTAGAAGCTGTTTC
AAATGCTGTATTAAGAGCAAGAGCTGGTATGAAAGATCCTCAAAAACCAATAGGATCATTTATATTTTTGGGACCAACTG
GAGTTGGTAAGACTGAATTAGCTAAAACTCTATGCAAAAATTTATTTGATAGTGAAGAAAATATAATAAGAATAGATATG
TCAGAGTATATGGAAAAGTATTCTGTATCCAGATTAATCGGAGCTCCTCCAGGATATGTAGGATATGAAGAAGGTGGTCA
ACTTACAGAGGCAGTTAGAAGAAAACCTTATTCTGTAATACTTTTCGATGAAATAGAAAAAGCTCATGATGATGTGTTTA
ATATATTCTTGCAAATATTAGATGATGGAAGATTAACAGATAATAAAGGAAAAACAGTTGACTTTAAGAATTGTATAATA
ATAATGACATCTAATATAGGTAGTTCCTATCTTTTAGAAAATAAAAAAGAAGACGGAATAGATGAAACAGTTAAAAATAA
AGTATCTAATGCCTTAAAGGATAGATTTAAACCAGAATTTTTAAATAGATTAGATGATATAATAATGTTTAAACCACTTA
CAAATAGAGAAATAACTAAAATAATAGATATATTCCTACAAGATATTGAAAATAGGCTAAAAGATAGAAATATAACTTTG
ATTGTAACAGAAAATGCGAAAGAACTTATGGCAAAAGAAGGATATGATGCAATTTATGGAGCTAGACCTTTAAAAAGATA
TATTGAAAATATATTAGAAACTAAAATAGCAAAACAAATAATAAAAGGAGATATATACGAAGGATGTAAAATAGGAGTAG
ACATAAAAGGTGAAGAAATAATAATAGGAAAAATATAA

Upstream 100 bases:

>100_bases
GACTATTGTTTTAATATAAATATAGGTAAATATAGGGACAGGTCGCAAAAATTAAGTACAAAACTTTTGTAACCTGTGCT
CAATAAGGAGGTATATATAT

Downstream 100 bases:

>100_bases
TAAATAAAAGCTACAGCTTTAACTTTATAAATTTTAAAAAGTTAAAGTTGTGGCTTTTTTTATTAAAAGTATAAAAACTT
ACCTAAAGAAGTTATATAGA

Product: clpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MDIEKLTLKVQQTINDSQKIAVKYNHQQLEPFHLFAALVFQEDGLIPNILGKMNINIKVLRDEIKRELNEMPKVLGDGAQ
NSGVYATRSFEEIFIRAESIAKDFKDSYISVEHIMLSLMQGRSTSIKKILDKFNIRKDKFLNVLQQVRGNQRVDTQDPEG
TYEALVKYGRNLIEDAKKHKLDPVIGRDEEIRRIVRILSRRTKNNPVLIGDPGVGKTAIIEGLAERIVRGDVPEGLKNKI
IFSLDMGALIAGAKFRGEFEERLKAVLKEVENSQGKIILFIDEIHNIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTF
DEYRKYIEKDKALERRFQPVIIDEPTVEDTISIIRGLKERFEIHHGIRIHDSAIVAAAKLSQRYITDRYLPDKAIDLIDE
AGAMIRTEIDSLPTELDSIKRKIFQMEIEKEALAKEKDSRSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLK
QKLDEVKGQLEKAEREYDLNKVAELKYGIIPGIKSQIEEKEILIKENSQGNMLKEEVTENEISKIISHWTGIPVTKLIEG
EKDKLLRLEDELKSRVIGQDEAVEAVSNAVLRARAGMKDPQKPIGSFIFLGPTGVGKTELAKTLCKNLFDSEENIIRIDM
SEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKTVDFKNCII
IMTSNIGSSYLLENKKEDGIDETVKNKVSNALKDRFKPEFLNRLDDIIMFKPLTNREITKIIDIFLQDIENRLKDRNITL
IVTENAKELMAKEGYDAIYGARPLKRYIENILETKIAKQIIKGDIYEGCKIGVDIKGEEIIIGKI

Sequences:

>Translated_865_residues
MDIEKLTLKVQQTINDSQKIAVKYNHQQLEPFHLFAALVFQEDGLIPNILGKMNINIKVLRDEIKRELNEMPKVLGDGAQ
NSGVYATRSFEEIFIRAESIAKDFKDSYISVEHIMLSLMQGRSTSIKKILDKFNIRKDKFLNVLQQVRGNQRVDTQDPEG
TYEALVKYGRNLIEDAKKHKLDPVIGRDEEIRRIVRILSRRTKNNPVLIGDPGVGKTAIIEGLAERIVRGDVPEGLKNKI
IFSLDMGALIAGAKFRGEFEERLKAVLKEVENSQGKIILFIDEIHNIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTF
DEYRKYIEKDKALERRFQPVIIDEPTVEDTISIIRGLKERFEIHHGIRIHDSAIVAAAKLSQRYITDRYLPDKAIDLIDE
AGAMIRTEIDSLPTELDSIKRKIFQMEIEKEALAKEKDSRSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLK
QKLDEVKGQLEKAEREYDLNKVAELKYGIIPGIKSQIEEKEILIKENSQGNMLKEEVTENEISKIISHWTGIPVTKLIEG
EKDKLLRLEDELKSRVIGQDEAVEAVSNAVLRARAGMKDPQKPIGSFIFLGPTGVGKTELAKTLCKNLFDSEENIIRIDM
SEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKTVDFKNCII
IMTSNIGSSYLLENKKEDGIDETVKNKVSNALKDRFKPEFLNRLDDIIMFKPLTNREITKIIDIFLQDIENRLKDRNITL
IVTENAKELMAKEGYDAIYGARPLKRYIENILETKIAKQIIKGDIYEGCKIGVDIKGEEIIIGKI
>Mature_865_residues
MDIEKLTLKVQQTINDSQKIAVKYNHQQLEPFHLFAALVFQEDGLIPNILGKMNINIKVLRDEIKRELNEMPKVLGDGAQ
NSGVYATRSFEEIFIRAESIAKDFKDSYISVEHIMLSLMQGRSTSIKKILDKFNIRKDKFLNVLQQVRGNQRVDTQDPEG
TYEALVKYGRNLIEDAKKHKLDPVIGRDEEIRRIVRILSRRTKNNPVLIGDPGVGKTAIIEGLAERIVRGDVPEGLKNKI
IFSLDMGALIAGAKFRGEFEERLKAVLKEVENSQGKIILFIDEIHNIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTF
DEYRKYIEKDKALERRFQPVIIDEPTVEDTISIIRGLKERFEIHHGIRIHDSAIVAAAKLSQRYITDRYLPDKAIDLIDE
AGAMIRTEIDSLPTELDSIKRKIFQMEIEKEALAKEKDSRSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLK
QKLDEVKGQLEKAEREYDLNKVAELKYGIIPGIKSQIEEKEILIKENSQGNMLKEEVTENEISKIISHWTGIPVTKLIEG
EKDKLLRLEDELKSRVIGQDEAVEAVSNAVLRARAGMKDPQKPIGSFIFLGPTGVGKTELAKTLCKNLFDSEENIIRIDM
SEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKTVDFKNCII
IMTSNIGSSYLLENKKEDGIDETVKNKVSNALKDRFKPEFLNRLDDIIMFKPLTNREITKIIDIFLQDIENRLKDRNITL
IVTENAKELMAKEGYDAIYGARPLKRYIENILETKIAKQIIKGDIYEGCKIGVDIKGEEIIIGKI

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=319, Percent_Identity=36.6771159874608, Blast_Score=210, Evalue=4e-54,
Organism=Escherichia coli, GI1788943, Length=862, Percent_Identity=51.2761020881671, Blast_Score=901, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=412, Percent_Identity=37.8640776699029, Blast_Score=284, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=714, Percent_Identity=52.9411764705882, Blast_Score=751, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=854, Percent_Identity=44.0281030444965, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_CLOTE (Q898C7)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_781219.1
- ProteinModelPortal:   Q898C7
- GeneID:   1060156
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00536
- NMPDR:   fig|212717.1.peg.441
- HOGENOM:   HBG413133
- OMA:   PNILGKM
- PhylomeDB:   Q898C7
- ProtClustDB:   CLSK882651
- BioCyc:   CTET212717:CTC_00536-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382
- TIGRFAMs:   TIGR03346

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 98581; Mature: 98581

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEKLTLKVQQTINDSQKIAVKYNHQQLEPFHLFAALVFQEDGLIPNILGKMNINIKVL
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHH
RDEIKRELNEMPKVLGDGAQNSGVYATRSFEEIFIRAESIAKDFKDSYISVEHIMLSLMQ
HHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSTSIKKILDKFNIRKDKFLNVLQQVRGNQRVDTQDPEGTYEALVKYGRNLIEDAKKHK
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LDPVIGRDEEIRRIVRILSRRTKNNPVLIGDPGVGKTAIIEGLAERIVRGDVPEGLKNKI
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCE
IFSLDMGALIAGAKFRGEFEERLKAVLKEVENSQGKIILFIDEIHNIVGAGKTEGSMDAG
EEEECHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCC
NLIKPMLARGELNCIGATTFDEYRKYIEKDKALERRFQPVIIDEPTVEDTISIIRGLKER
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHH
FEIHHGIRIHDSAIVAAAKLSQRYITDRYLPDKAIDLIDEAGAMIRTEIDSLPTELDSIK
HHHHCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RKIFQMEIEKEALAKEKDSRSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLDEVKGQLEKAEREYDLNKVAELKYGIIPGIKSQIEEKEILIKENSQGNMLKEEVTEN
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHCHHHEEEEECCCCCHHHHHHHHH
EISKIISHWTGIPVTKLIEGEKDKLLRLEDELKSRVIGQDEAVEAVSNAVLRARAGMKDP
HHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
QKPIGSFIFLGPTGVGKTELAKTLCKNLFDSEENIIRIDMSEYMEKYSVSRLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKTVDFKNCII
CCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEE
IMTSNIGSSYLLENKKEDGIDETVKNKVSNALKDRFKPEFLNRLDDIIMFKPLTNREITK
EEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHH
IIDIFLQDIENRLKDRNITLIVTENAKELMAKEGYDAIYGARPLKRYIENILETKIAKQI
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHH
IKGDIYEGCKIGVDIKGEEIIIGKI
HHCCCCCCCEECEEECCCEEEEECC
>Mature Secondary Structure
MDIEKLTLKVQQTINDSQKIAVKYNHQQLEPFHLFAALVFQEDGLIPNILGKMNINIKVL
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHH
RDEIKRELNEMPKVLGDGAQNSGVYATRSFEEIFIRAESIAKDFKDSYISVEHIMLSLMQ
HHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRSTSIKKILDKFNIRKDKFLNVLQQVRGNQRVDTQDPEGTYEALVKYGRNLIEDAKKHK
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
LDPVIGRDEEIRRIVRILSRRTKNNPVLIGDPGVGKTAIIEGLAERIVRGDVPEGLKNKI
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCE
IFSLDMGALIAGAKFRGEFEERLKAVLKEVENSQGKIILFIDEIHNIVGAGKTEGSMDAG
EEEECHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCC
NLIKPMLARGELNCIGATTFDEYRKYIEKDKALERRFQPVIIDEPTVEDTISIIRGLKER
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHH
FEIHHGIRIHDSAIVAAAKLSQRYITDRYLPDKAIDLIDEAGAMIRTEIDSLPTELDSIK
HHHHCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RKIFQMEIEKEALAKEKDSRSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLDEVKGQLEKAEREYDLNKVAELKYGIIPGIKSQIEEKEILIKENSQGNMLKEEVTEN
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHCHHHEEEEECCCCCHHHHHHHHH
EISKIISHWTGIPVTKLIEGEKDKLLRLEDELKSRVIGQDEAVEAVSNAVLRARAGMKDP
HHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
QKPIGSFIFLGPTGVGKTELAKTLCKNLFDSEENIIRIDMSEYMEKYSVSRLIGAPPGYV
CCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCC
GYEEGGQLTEAVRRKPYSVILFDEIEKAHDDVFNIFLQILDDGRLTDNKGKTVDFKNCII
CCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCEEE
IMTSNIGSSYLLENKKEDGIDETVKNKVSNALKDRFKPEFLNRLDDIIMFKPLTNREITK
EEECCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCHHHHH
IIDIFLQDIENRLKDRNITLIVTENAKELMAKEGYDAIYGARPLKRYIENILETKIAKQI
HHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHH
IKGDIYEGCKIGVDIKGEEIIIGKI
HHCCCCCCCEECEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129