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The map label for this gene is iolD [H]

Identifier: 28210252

GI number: 28210252

Start: 516777

End: 518744

Strand: Direct

Name: iolD [H]

Synonym: CTC00510

Alternate gene names: 28210252

Gene position: 516777-518744 (Clockwise)

Preceding gene: 28210251

Following gene: 28210253

Centisome position: 18.46

GC content: 33.33

Gene sequence:

>1968_bases
TTGGCCTCAACAATAGTAAAAGGAGGAATTATGGTGGAAACTATCAAGATAACAACTGCTCAAGCACTTATAAAATTTTT
AAATCAACAATATGTAGAATTAGATGGACAGGAGTATAGATTTGTACAAGGAATTTTCACAATATTCGGACACGGCAATG
TTTTAGGAATAGGACAAGCACTAGAAGAAGATCCAGGTCATTTAGAAGTTTATCAAGGACATAACGAACAGGGAATGGCT
CAATCAGCTATAGCCTTTGCAAAACAAAGTAATAGAAAACAAATATATGCGTGCACATCATCTGTTGGACCAGGTGCTGC
TAATATGGTAACAGCAGCGGCTACAGCTACAGCAAATAATATACCTGTATTGTTGTTACCAGGGGATACTTTTTCAACAA
GACAACCAGATCCAGTGCTCCAACAGGTAGAGCAAACATATAACTTATCTATTACTACCAATGATGCCTTTAAGGCAGTA
AGCAAATATTGGGACAGAGTAAATAGACCAGAACAGCTTATGACAGCTATGATAAATGCTATGAGAGTATTAACAGATCC
TGCAAATACAGGAGCAGTAACCATAGCATTACCTCAAGATGTGCAAGGAGAAATTTATGATTTCCCAGAATATTTTTTTA
AAAAACGTGTTCATAGAATAGAAAGAACACCTCCTTCAAAACAAGCCATAGAAGATGCTGTAGAGCTTATAAAAAGAAAA
AAGAAGCCATTAATAATATGTGGTGGTGGTGTTAGGTATTCAGAAGCAGCAGAATCACTTAAAAGATTTTCAGAAAAGTT
TAATATTCCTTTTGGAGAGACGCAGGCGGGTAAAAGTGCAATTGAGTGGAGTTATGGATTAAATCTAGGAGGAATAGGGG
TAACTGGAAATTCTGCAGCTAATAGTATAGCAAAAGATGCAGACTTGATAATAGGAGTAGGAACTAGATTTACTGATTTT
ACAACTTGTTCAAAGTTTTTATTTCAAAATGATGATGTAGAGTTTTTAACTATAAATATATCTAGTTTCCATGCTAATAA
ATTAGATGCGCTAAAAGTTATTTCAGATGCAAAAGTAGGACTAGATACAATAGCAAATGAGCTAGAAAGACAAGGATATT
CTTCAGATTATGAAGATGAAATAAAAAAAGCTAAAAATGAATGGGAAAAAGAACTTAATAGGCTTTTTAATATAGAGTAT
ACAGAAAAAGAGTTTGTACCTGAAATAGCAGGTCATTGTGATAAGGTAGTAGAGGAGTTTTATAAAGAATTTGATTCCTG
TTTAACACAGACAAAGGTTTTGGGAGAACTAAATGAGCTTTTAGATGATGATGCAATTGTAATAGGAGCTTCGGGAAGCT
TACCAGGAGACCTACATAAGGTATGGTGTCCTAAAAGGAGTAATACTTATCATATGGAGTATGGATATTCTTGTATGGGG
TATGAAGTTTCTGCTGCATTAGGAGTTAAATTGGCAGAAGAAGATAAGGAGGTTTATTCCTTGGTAGGAGATGGAGCGTA
TTTAATGCTTCACTCGGAGTTAATTACTAGTATAAAAGAAGGAAAGAAAATAAATATATTGTTGTTTGACAATGCAGGTT
TTGGATGTATAAATAATCTTCAAATGTCTAATGGTATGGGAAGCTTTGCTACTGAATTTAGACATAGGAATAGTGAAACA
GGAAAACTTGATGGAAAGCTTTTAAAAATTGATTTTGCAAAATCTGCTGAAGGATATGGGGTTAAAACTTATAAAGTAAA
TACAATAGATAAGCTTAGATATGCAATAGAAGATTCTAAAAAGCAAAAAATATCTACATTAATTGATATAAAAATACTAC
CTAAAACTATGACAAGGGGATATGAATCCTGGTGGCATGTTGGGGTATCAGAAAAATCAAAAAATCCTAAAATAAATAAA
GCGTATGAAAGTAAAATAAACCATTTAGCCAAAGCTAGAAAATATTAA

Upstream 100 bases:

>100_bases
TGTATTATTGTTGGATGATAAGACATTTAGAAAACAATCCTTGGACAGATAGGATTAATGAAAAAGAGCATGAGTGGCTT
TTAGAATCAGATGTTAAGAT

Downstream 100 bases:

>100_bases
ATCAATAATAATTTAATTCAAAGATAAAAAAGGGGATGAAGGTAATGATTAATTTAGGAGTTATTGGATTTGGAAGAATA
GGTAAGGTACATGCAGAAAG

Product: myo-inositol catabolism protein iolD

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MASTIVKGGIMVETIKITTAQALIKFLNQQYVELDGQEYRFVQGIFTIFGHGNVLGIGQALEEDPGHLEVYQGHNEQGMA
QSAIAFAKQSNRKQIYACTSSVGPGAANMVTAAATATANNIPVLLLPGDTFSTRQPDPVLQQVEQTYNLSITTNDAFKAV
SKYWDRVNRPEQLMTAMINAMRVLTDPANTGAVTIALPQDVQGEIYDFPEYFFKKRVHRIERTPPSKQAIEDAVELIKRK
KKPLIICGGGVRYSEAAESLKRFSEKFNIPFGETQAGKSAIEWSYGLNLGGIGVTGNSAANSIAKDADLIIGVGTRFTDF
TTCSKFLFQNDDVEFLTINISSFHANKLDALKVISDAKVGLDTIANELERQGYSSDYEDEIKKAKNEWEKELNRLFNIEY
TEKEFVPEIAGHCDKVVEEFYKEFDSCLTQTKVLGELNELLDDDAIVIGASGSLPGDLHKVWCPKRSNTYHMEYGYSCMG
YEVSAALGVKLAEEDKEVYSLVGDGAYLMLHSELITSIKEGKKINILLFDNAGFGCINNLQMSNGMGSFATEFRHRNSET
GKLDGKLLKIDFAKSAEGYGVKTYKVNTIDKLRYAIEDSKKQKISTLIDIKILPKTMTRGYESWWHVGVSEKSKNPKINK
AYESKINHLAKARKY

Sequences:

>Translated_655_residues
MASTIVKGGIMVETIKITTAQALIKFLNQQYVELDGQEYRFVQGIFTIFGHGNVLGIGQALEEDPGHLEVYQGHNEQGMA
QSAIAFAKQSNRKQIYACTSSVGPGAANMVTAAATATANNIPVLLLPGDTFSTRQPDPVLQQVEQTYNLSITTNDAFKAV
SKYWDRVNRPEQLMTAMINAMRVLTDPANTGAVTIALPQDVQGEIYDFPEYFFKKRVHRIERTPPSKQAIEDAVELIKRK
KKPLIICGGGVRYSEAAESLKRFSEKFNIPFGETQAGKSAIEWSYGLNLGGIGVTGNSAANSIAKDADLIIGVGTRFTDF
TTCSKFLFQNDDVEFLTINISSFHANKLDALKVISDAKVGLDTIANELERQGYSSDYEDEIKKAKNEWEKELNRLFNIEY
TEKEFVPEIAGHCDKVVEEFYKEFDSCLTQTKVLGELNELLDDDAIVIGASGSLPGDLHKVWCPKRSNTYHMEYGYSCMG
YEVSAALGVKLAEEDKEVYSLVGDGAYLMLHSELITSIKEGKKINILLFDNAGFGCINNLQMSNGMGSFATEFRHRNSET
GKLDGKLLKIDFAKSAEGYGVKTYKVNTIDKLRYAIEDSKKQKISTLIDIKILPKTMTRGYESWWHVGVSEKSKNPKINK
AYESKINHLAKARKY
>Mature_654_residues
ASTIVKGGIMVETIKITTAQALIKFLNQQYVELDGQEYRFVQGIFTIFGHGNVLGIGQALEEDPGHLEVYQGHNEQGMAQ
SAIAFAKQSNRKQIYACTSSVGPGAANMVTAAATATANNIPVLLLPGDTFSTRQPDPVLQQVEQTYNLSITTNDAFKAVS
KYWDRVNRPEQLMTAMINAMRVLTDPANTGAVTIALPQDVQGEIYDFPEYFFKKRVHRIERTPPSKQAIEDAVELIKRKK
KPLIICGGGVRYSEAAESLKRFSEKFNIPFGETQAGKSAIEWSYGLNLGGIGVTGNSAANSIAKDADLIIGVGTRFTDFT
TCSKFLFQNDDVEFLTINISSFHANKLDALKVISDAKVGLDTIANELERQGYSSDYEDEIKKAKNEWEKELNRLFNIEYT
EKEFVPEIAGHCDKVVEEFYKEFDSCLTQTKVLGELNELLDDDAIVIGASGSLPGDLHKVWCPKRSNTYHMEYGYSCMGY
EVSAALGVKLAEEDKEVYSLVGDGAYLMLHSELITSIKEGKKINILLFDNAGFGCINNLQMSNGMGSFATEFRHRNSETG
KLDGKLLKIDFAKSAEGYGVKTYKVNTIDKLRYAIEDSKKQKISTLIDIKILPKTMTRGYESWWHVGVSEKSKNPKINKA
YESKINHLAKARKY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=19.8230088495575, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790104, Length=613, Percent_Identity=23.8172920065253, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786717, Length=525, Percent_Identity=25.3333333333333, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=24.8287671232877, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788716, Length=530, Percent_Identity=21.6981132075472, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787096, Length=527, Percent_Identity=20.6831119544592, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=619, Percent_Identity=24.232633279483, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922626, Length=595, Percent_Identity=20, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 72818; Mature: 72686

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTIVKGGIMVETIKITTAQALIKFLNQQYVELDGQEYRFVQGIFTIFGHGNVLGIGQA
CCCCEEECCEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCHH
LEEDPGHLEVYQGHNEQGMAQSAIAFAKQSNRKQIYACTSSVGPGAANMVTAAATATANN
HHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
IPVLLLPGDTFSTRQPDPVLQQVEQTYNLSITTNDAFKAVSKYWDRVNRPEQLMTAMINA
CEEEEECCCCCCCCCCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHH
MRVLTDPANTGAVTIALPQDVQGEIYDFPEYFFKKRVHRIERTPPSKQAIEDAVELIKRK
HHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
KKPLIICGGGVRYSEAAESLKRFSEKFNIPFGETQAGKSAIEWSYGLNLGGIGVTGNSAA
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCEECCCCHHH
NSIAKDADLIIGVGTRFTDFTTCSKFLFQNDDVEFLTINISSFHANKLDALKVISDAKVG
HHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCC
LDTIANELERQGYSSDYEDEIKKAKNEWEKELNRLFNIEYTEKEFVPEIAGHCDKVVEEF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
YKEFDSCLTQTKVLGELNELLDDDAIVIGASGSLPGDLHKVWCPKRSNTYHMEYGYSCMG
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEECCCEEEC
YEVSAALGVKLAEEDKEVYSLVGDGAYLMLHSELITSIKEGKKINILLFDNAGFGCINNL
EEEHHHHCCEEHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
QMSNGMGSFATEFRHRNSETGKLDGKLLKIDFAKSAEGYGVKTYKVNTIDKLRYAIEDSK
CCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHH
KQKISTLIDIKILPKTMTRGYESWWHVGVSEKSKNPKINKAYESKINHLAKARKY
HHHHHHHEEEEEECHHHHCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASTIVKGGIMVETIKITTAQALIKFLNQQYVELDGQEYRFVQGIFTIFGHGNVLGIGQA
CCCEEECCEEEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCHH
LEEDPGHLEVYQGHNEQGMAQSAIAFAKQSNRKQIYACTSSVGPGAANMVTAAATATANN
HHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
IPVLLLPGDTFSTRQPDPVLQQVEQTYNLSITTNDAFKAVSKYWDRVNRPEQLMTAMINA
CEEEEECCCCCCCCCCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHH
MRVLTDPANTGAVTIALPQDVQGEIYDFPEYFFKKRVHRIERTPPSKQAIEDAVELIKRK
HHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
KKPLIICGGGVRYSEAAESLKRFSEKFNIPFGETQAGKSAIEWSYGLNLGGIGVTGNSAA
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCEECCCCHHH
NSIAKDADLIIGVGTRFTDFTTCSKFLFQNDDVEFLTINISSFHANKLDALKVISDAKVG
HHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCC
LDTIANELERQGYSSDYEDEIKKAKNEWEKELNRLFNIEYTEKEFVPEIAGHCDKVVEEF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
YKEFDSCLTQTKVLGELNELLDDDAIVIGASGSLPGDLHKVWCPKRSNTYHMEYGYSCMG
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEECCCEEEC
YEVSAALGVKLAEEDKEVYSLVGDGAYLMLHSELITSIKEGKKINILLFDNAGFGCINNL
EEEHHHHCCEEHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
QMSNGMGSFATEFRHRNSETGKLDGKLLKIDFAKSAEGYGVKTYKVNTIDKLRYAIEDSK
CCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHH
KQKISTLIDIKILPKTMTRGYESWWHVGVSEKSKNPKINKAYESKINHLAKARKY
HHHHHHHEEEEEECHHHHCCCHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]