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The map label for this gene is topB [H]

Identifier: 28210156

GI number: 28210156

Start: 396524

End: 398716

Strand: Direct

Name: topB [H]

Synonym: CTC00403

Alternate gene names: 28210156

Gene position: 396524-398716 (Clockwise)

Preceding gene: 28210155

Following gene: 28210158

Centisome position: 14.17

GC content: 30.37

Gene sequence:

>2193_bases
ATGGGAAAAACATTAGTTTTGGCGGAAAAGCCAAGTGTAGGTAGAGAGATAGCTAGAGTATTAAAATGTAATAAAAACTC
TAGAAGTTATATAGAAAGTAATAAATATGTTGTTACTTGGGCATTAGGACACTTAGTGACTCTTGCTGATCCAGAAGTAT
ATGATAATAAGTATAAAAAATGGGAAATGGAGACGCTTCCAATGCTTCCAAAATACATGAAACTTTGTGTTATAGGTAAA
ACTTCAAAACAATTTAATGAAGTTAAAAAGCTTATGAACAGAAATGATATAGATGAAATTGTTATAGCTACAGATGCTGG
AAGAGAAGGGGAACTAGTAGCTAGGTGGATAATAGAAAAGGCAAGAGTTAATAAGCCAATTAAGAGACTTTGGATATCTT
CCCAAACTGATAAAGCCATAATAGATGGTTTTAATAATTTAAAACCAGGCAAAGATTATGAAAATCTATATAAATCAGCT
GTATGCAGATCAGAGGCAGACTGGTTAGTTGGTCTTAATGTAACAAGAGCATTGACCTGTAAATATAATGCTCAGCTTTC
TGCAGGAAGAGTTCAAACACCAACTCTTGCATTAATAGTAGAAAGAGAAGAGGAAATTAAAAACTTCAAACCACGAGATT
ATTATAATATAGTTGCTAAGACTAAGGGAATTCAATTTAATTGGCAAGATAAAAAAGGACAAATGGCTACATTTAACGAA
GATTTTGCAAATAATATCTTAGCTAAGGTTAGTGGGGAAATTGGTAATGTTACGGAAGTTATAGAGAAGGATAAAAAGCA
ATATGCTCCAGCTCTTTATGACTTAACAGAACTTCAAAGAGATGCTAATAGAATATTTGGATACTCTGCAAAGGAAACTC
TTTCTATAATGCAAAGATTATATGAGAACCATAAAGTACTAACCTACCCAAGAACTGATTCAAGATATATATCAAAAGAT
ATAGTAGCGACTATACCTCAAAGATTAAAGAGTATATCTATAGGAATTTACAGTAAATTTGCAGAAGAAATACTAAGAGG
AAAAATCGTAGCACATAAAGGATTTGTAGATGATAGTAAGGTTTCAGACCATCATGCTATAATTCCAACAGAAGAAAGGG
TTAAATTATCAAATCTAAGTGGTGATGAAAGAAAAATATATGATTTAGTTATAAAGAGATTTTTAAGTGTAATGCTTCCA
CCTTTTGAATACATCCAAACAACTATTAAAGTAGAAGTTAATGGAGAAGTCTTTATAGCAAAGGGAAAAAATATAAAACA
TAATGGATGGAAAGCTGTATATAATAAAGAAGATTTAAACGAAGATAATAATGATGGAGATGGGCTTAAAGAGCAGGTTT
TGCCTCAAATTATTAAAGGAGATGCTATAAAATTTAACTTAGTTAATATAAATAAAGGTCAAACAAAACCGCCAGCTAGA
TTTAACGAAGGAACATTATTATCTGCAATGGAAAATCCTCAAAAATATATAAAATTAAATAAAGAATATGCAAAGACTTT
AGGGGAAACTGGTGGACTTGGTACAGTAGCTACAAGAGCAGATATAATAGAAAAGTTATATAATTCTTTTTATATAGAGA
AAAAGGGTAAAGAGATAATACCTACATCAAAAGGAAAACAAATTATAGATTTAGTGCCACAGGACTTAAAATCTCCTATA
TTAACAGCGGAATGGGAGAAAAAATTAGAATTAATAAGTAAAGGTAAAATTAGTGGTAAAGAATTCATAAACAACATGAG
AAATTACTCAACAAAACTTGTACAGGATGTAAAAAATAGTAATGAAAAATATATACACAATAATTTGACAGGTACAAAAT
GCCCTAATTGTGAAAAGTATATGCTTGAAGTTAATGGGAAACGAGGTAAAATACTTGTATGCCAAGATAGAGAATGTGGA
TACAGAGAAAATATTAGTAGATTCACTAATGTAAGATGTCCTGAGTGCCATAAAAAACTTGAATTAAGGGGAGAAGGGGA
AGGACAAATTTATGTGTGTTCAAAATGTACATTTAGAGAAAAACTTTCATCCTTTAATAAGAGATTTAAAGAAAAAGGAA
ATAATGCAGATAAAAGAGAAGTATCAAGATATATGAAAAAGATGAAAGAACAGGATAAAGAACCTATGAATTCAGCCCTT
GCAGATGCATTAGCAAAACTAAACTTAAAATAA

Upstream 100 bases:

>100_bases
TCAAGGAAACATGTAAGCTATATAATAGTATGAGAAAATAAGCAGGTTTAATTTAACTATCCTTATTTTATGTAAATTAA
AGTATGAGAGGAAGTAAATA

Downstream 100 bases:

>100_bases
TAAAATATAATTTACTACAGTAAAGAGTAAAAGTGACCAAGGAATTCAAAAAATAATGAACTTCTTAGTCACTTTTACGA
AATATTATAAAAATTTAATA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 730; Mature: 729

Protein sequence:

>730_residues
MGKTLVLAEKPSVGREIARVLKCNKNSRSYIESNKYVVTWALGHLVTLADPEVYDNKYKKWEMETLPMLPKYMKLCVIGK
TSKQFNEVKKLMNRNDIDEIVIATDAGREGELVARWIIEKARVNKPIKRLWISSQTDKAIIDGFNNLKPGKDYENLYKSA
VCRSEADWLVGLNVTRALTCKYNAQLSAGRVQTPTLALIVEREEEIKNFKPRDYYNIVAKTKGIQFNWQDKKGQMATFNE
DFANNILAKVSGEIGNVTEVIEKDKKQYAPALYDLTELQRDANRIFGYSAKETLSIMQRLYENHKVLTYPRTDSRYISKD
IVATIPQRLKSISIGIYSKFAEEILRGKIVAHKGFVDDSKVSDHHAIIPTEERVKLSNLSGDERKIYDLVIKRFLSVMLP
PFEYIQTTIKVEVNGEVFIAKGKNIKHNGWKAVYNKEDLNEDNNDGDGLKEQVLPQIIKGDAIKFNLVNINKGQTKPPAR
FNEGTLLSAMENPQKYIKLNKEYAKTLGETGGLGTVATRADIIEKLYNSFYIEKKGKEIIPTSKGKQIIDLVPQDLKSPI
LTAEWEKKLELISKGKISGKEFINNMRNYSTKLVQDVKNSNEKYIHNNLTGTKCPNCEKYMLEVNGKRGKILVCQDRECG
YRENISRFTNVRCPECHKKLELRGEGEGQIYVCSKCTFREKLSSFNKRFKEKGNNADKREVSRYMKKMKEQDKEPMNSAL
ADALAKLNLK

Sequences:

>Translated_730_residues
MGKTLVLAEKPSVGREIARVLKCNKNSRSYIESNKYVVTWALGHLVTLADPEVYDNKYKKWEMETLPMLPKYMKLCVIGK
TSKQFNEVKKLMNRNDIDEIVIATDAGREGELVARWIIEKARVNKPIKRLWISSQTDKAIIDGFNNLKPGKDYENLYKSA
VCRSEADWLVGLNVTRALTCKYNAQLSAGRVQTPTLALIVEREEEIKNFKPRDYYNIVAKTKGIQFNWQDKKGQMATFNE
DFANNILAKVSGEIGNVTEVIEKDKKQYAPALYDLTELQRDANRIFGYSAKETLSIMQRLYENHKVLTYPRTDSRYISKD
IVATIPQRLKSISIGIYSKFAEEILRGKIVAHKGFVDDSKVSDHHAIIPTEERVKLSNLSGDERKIYDLVIKRFLSVMLP
PFEYIQTTIKVEVNGEVFIAKGKNIKHNGWKAVYNKEDLNEDNNDGDGLKEQVLPQIIKGDAIKFNLVNINKGQTKPPAR
FNEGTLLSAMENPQKYIKLNKEYAKTLGETGGLGTVATRADIIEKLYNSFYIEKKGKEIIPTSKGKQIIDLVPQDLKSPI
LTAEWEKKLELISKGKISGKEFINNMRNYSTKLVQDVKNSNEKYIHNNLTGTKCPNCEKYMLEVNGKRGKILVCQDRECG
YRENISRFTNVRCPECHKKLELRGEGEGQIYVCSKCTFREKLSSFNKRFKEKGNNADKREVSRYMKKMKEQDKEPMNSAL
ADALAKLNLK
>Mature_729_residues
GKTLVLAEKPSVGREIARVLKCNKNSRSYIESNKYVVTWALGHLVTLADPEVYDNKYKKWEMETLPMLPKYMKLCVIGKT
SKQFNEVKKLMNRNDIDEIVIATDAGREGELVARWIIEKARVNKPIKRLWISSQTDKAIIDGFNNLKPGKDYENLYKSAV
CRSEADWLVGLNVTRALTCKYNAQLSAGRVQTPTLALIVEREEEIKNFKPRDYYNIVAKTKGIQFNWQDKKGQMATFNED
FANNILAKVSGEIGNVTEVIEKDKKQYAPALYDLTELQRDANRIFGYSAKETLSIMQRLYENHKVLTYPRTDSRYISKDI
VATIPQRLKSISIGIYSKFAEEILRGKIVAHKGFVDDSKVSDHHAIIPTEERVKLSNLSGDERKIYDLVIKRFLSVMLPP
FEYIQTTIKVEVNGEVFIAKGKNIKHNGWKAVYNKEDLNEDNNDGDGLKEQVLPQIIKGDAIKFNLVNINKGQTKPPARF
NEGTLLSAMENPQKYIKLNKEYAKTLGETGGLGTVATRADIIEKLYNSFYIEKKGKEIIPTSKGKQIIDLVPQDLKSPIL
TAEWEKKLELISKGKISGKEFINNMRNYSTKLVQDVKNSNEKYIHNNLTGTKCPNCEKYMLEVNGKRGKILVCQDRECGY
RENISRFTNVRCPECHKKLELRGEGEGQIYVCSKCTFREKLSSFNKRFKEKGNNADKREVSRYMKKMKEQDKEPMNSALA
DALAKLNLK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=691, Percent_Identity=25.0361794500724, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI4507635, Length=751, Percent_Identity=24.3675099866844, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=32.5281803542673, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1787529, Length=677, Percent_Identity=26.2924667651403, Blast_Score=158, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32563869, Length=696, Percent_Identity=24.2816091954023, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17555378, Length=627, Percent_Identity=24.7208931419458, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=589, Percent_Identity=23.0899830220713, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24640096, Length=713, Percent_Identity=22.8611500701262, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24585251, Length=572, Percent_Identity=25, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83507; Mature: 83376

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTLVLAEKPSVGREIARVLKCNKNSRSYIESNKYVVTWALGHLVTLADPEVYDNKYKK
CCCEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCEEEECCCHHHCCCCCE
WEMETLPMLPKYMKLCVIGKTSKQFNEVKKLMNRNDIDEIVIATDAGREGELVARWIIEK
ECCHHCCCCHHHHHHEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
ARVNKPIKRLWISSQTDKAIIDGFNNLKPGKDYENLYKSAVCRSEADWLVGLNVTRALTC
HHCCCHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCEEEEEEE
KYNAQLSAGRVQTPTLALIVEREEEIKNFKPRDYYNIVAKTKGIQFNWQDKKGQMATFNE
EECCCCCCCCCCCCEEEEEEECHHHHCCCCCCCHHHHEEECCCCEEEECCCCCCEEECCH
DFANNILAKVSGEIGNVTEVIEKDKKQYAPALYDLTELQRDANRIFGYSAKETLSIMQRL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKVLTYPRTDSRYISKDIVATIPQRLKSISIGIYSKFAEEILRGKIVAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
VSDHHAIIPTEERVKLSNLSGDERKIYDLVIKRFLSVMLPPFEYIQTTIKVEVNGEVFIA
CCCCCEECCCHHCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCEEEEE
KGKNIKHNGWKAVYNKEDLNEDNNDGDGLKEQVLPQIIKGDAIKFNLVNINKGQTKPPAR
ECCCCCCCCCEEECCHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
FNEGTLLSAMENPQKYIKLNKEYAKTLGETGGLGTVATRADIIEKLYNSFYIEKKGKEII
CCCCCEEHHHHCCHHHHEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCC
PTSKGKQIIDLVPQDLKSPILTAEWEKKLELISKGKISGKEFINNMRNYSTKLVQDVKNS
CCCCCCHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
NEKYIHNNLTGTKCPNCEKYMLEVNGKRGKILVCQDRECGYRENISRFTNVRCPECHKKL
CCCEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHC
ELRGEGEGQIYVCSKCTFREKLSSFNKRFKEKGNNADKREVSRYMKKMKEQDKEPMNSAL
EECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ADALAKLNLK
HHHHHHCCCC
>Mature Secondary Structure 
GKTLVLAEKPSVGREIARVLKCNKNSRSYIESNKYVVTWALGHLVTLADPEVYDNKYKK
CCEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCEEEECCCHHHCCCCCE
WEMETLPMLPKYMKLCVIGKTSKQFNEVKKLMNRNDIDEIVIATDAGREGELVARWIIEK
ECCHHCCCCHHHHHHEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
ARVNKPIKRLWISSQTDKAIIDGFNNLKPGKDYENLYKSAVCRSEADWLVGLNVTRALTC
HHCCCHHHHHHCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCEEEEEEE
KYNAQLSAGRVQTPTLALIVEREEEIKNFKPRDYYNIVAKTKGIQFNWQDKKGQMATFNE
EECCCCCCCCCCCCEEEEEEECHHHHCCCCCCCHHHHEEECCCCEEEECCCCCCEEECCH
DFANNILAKVSGEIGNVTEVIEKDKKQYAPALYDLTELQRDANRIFGYSAKETLSIMQRL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKVLTYPRTDSRYISKDIVATIPQRLKSISIGIYSKFAEEILRGKIVAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCC
VSDHHAIIPTEERVKLSNLSGDERKIYDLVIKRFLSVMLPPFEYIQTTIKVEVNGEVFIA
CCCCCEECCCHHCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEEEEEEECCEEEEE
KGKNIKHNGWKAVYNKEDLNEDNNDGDGLKEQVLPQIIKGDAIKFNLVNINKGQTKPPAR
ECCCCCCCCCEEECCHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
FNEGTLLSAMENPQKYIKLNKEYAKTLGETGGLGTVATRADIIEKLYNSFYIEKKGKEII
CCCCCEEHHHHCCHHHHEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCC
PTSKGKQIIDLVPQDLKSPILTAEWEKKLELISKGKISGKEFINNMRNYSTKLVQDVKNS
CCCCCCHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
NEKYIHNNLTGTKCPNCEKYMLEVNGKRGKILVCQDRECGYRENISRFTNVRCPECHKKL
CCCEEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHC
ELRGEGEGQIYVCSKCTFREKLSSFNKRFKEKGNNADKREVSRYMKKMKEQDKEPMNSAL
EECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHH
ADALAKLNLK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA