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The map label for this gene is rpoN [H]

Identifier: 28210132

GI number: 28210132

Start: 364944

End: 366329

Strand: Direct

Name: rpoN [H]

Synonym: CTC00376

Alternate gene names: 28210132

Gene position: 364944-366329 (Clockwise)

Preceding gene: 28210128

Following gene: 28210133

Centisome position: 13.04

GC content: 26.19

Gene sequence:

>1386_bases
ATGAAAAATGTGGATTTAGGGTTTGAGTTATCTATATCTCAAGAACAAAAATTAATAATGACTCATCAAATGCAGCTATC
AATAAAAATATTGCAACTTTCTTCTTATGAACTTACAAAACTTGTGGAGAAACAATTACAAGAAAATCCTCTTTTAGAAA
TAAATTATGAAGAAATAAAAAAAGAAGAGAGTTTGGAAGAGAATTTAGATGAAGTGTTAAAGTATAAAAATATTATAAAA
AATCTTCAAGATGATAATTATATAAACAATCAACATAGTGAAGAAGAAGTGAGTCCTTTTAATTTTATATCTAGTAAAAA
ATCTTTAAAAGAATATTTATTAGAGCAAATAATGTATTTAGATGAAAAGGAATACATTAAGTGTATATGTAATTACATAA
TTGAGGGTATAGATGAAAGAGGATATTTGGCTGAAGACATAGAATCTATTTCAAAAGAATTGAAAGTATCTATTAATTTA
GTAATTAAAGCATTAAATATAGTTCAAGATTTAGAGCCAGATGGTATTGGAGCTAGAGATTTAATTGAATGTTTAAGGAT
ACAATGTAATAAGCGGGAAATAAAGGATGAAAAAATATATAATATAATAGATAATCATTTAGAAGACATTGCAGAAAATA
AGTATTCTAACATAGGAAAGAAACTTGATATATCACCTAAAAAAGCACAGATGTATGGAGATTTTATAAAATCCTTACAG
CCTAAGCCATCTAGTGGATTTTATACAGGAGAAGAAGTAAGATACATAGTACCAGATGCATATATTAAAAAAATAGGACG
AGAATACGTTATATTAATGAATGAAAGTATAACACCTAAACTTACCATAAACAATTTGTACAAGAACATAATAAATAATA
ATGAAGATGAGGCCGCAGTGGAATATGTAAAGGAAAAGTTAAATAGTGCATCTTATCTAATAAAAAGTATAGAACAAAGG
AAAAGTACTATATATAGGGTTTTAGAAAAAATAATTGAAATACAAAGAGAATATTTTGATAAAGATAAAAAGCACTTAAA
ACCAATGACATTAAAAGAAATAGCAGAAGAATTAAAGGTGCATGAATCAACGGTTAGTAGAGCTATAAGAGAAAAATATA
TACATACAGATAAAGGAATTATAAAAATAAGAAATTTATTTACCACAGCTATTGAAAGTAAAGGTAACGAGGATAGCTTA
TCCAATATTGCAGTTAAAAACATGATAGAAAAGTTTATAGAAGAAGAGGATAAGAAAAAGCCTCTATCAGACCAAAATAT
AGCAGACAGATTAAAGAAAAAAGATGTGAATATTTCCAGAAGAACTGTGGCAAAATACAGAGAAGAAATGGGAATAAAAT
CTTCTATGGGTAGAAAGAGATATTAA

Upstream 100 bases:

>100_bases
CAATTTTATAAAAATTCTCGTAATTACCAGTATAATTTATATCATGATAGATTTTTATACATTACATTTTGGATAAATGT
TATAATAAAGTAGAAAGGGG

Downstream 100 bases:

>100_bases
AGTTTGAAGCGAGATTTTTCATAATTAAATTAATTTTTCATAAAAAATTTGATTTAACTATTGGAATTTATTGTGCTTTG
TTCTATAATAGAAGTGGGAT

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKNVDLGFELSISQEQKLIMTHQMQLSIKILQLSSYELTKLVEKQLQENPLLEINYEEIKKEESLEENLDEVLKYKNIIK
NLQDDNYINNQHSEEEVSPFNFISSKKSLKEYLLEQIMYLDEKEYIKCICNYIIEGIDERGYLAEDIESISKELKVSINL
VIKALNIVQDLEPDGIGARDLIECLRIQCNKREIKDEKIYNIIDNHLEDIAENKYSNIGKKLDISPKKAQMYGDFIKSLQ
PKPSSGFYTGEEVRYIVPDAYIKKIGREYVILMNESITPKLTINNLYKNIINNNEDEAAVEYVKEKLNSASYLIKSIEQR
KSTIYRVLEKIIEIQREYFDKDKKHLKPMTLKEIAEELKVHESTVSRAIREKYIHTDKGIIKIRNLFTTAIESKGNEDSL
SNIAVKNMIEKFIEEEDKKKPLSDQNIADRLKKKDVNISRRTVAKYREEMGIKSSMGRKRY

Sequences:

>Translated_461_residues
MKNVDLGFELSISQEQKLIMTHQMQLSIKILQLSSYELTKLVEKQLQENPLLEINYEEIKKEESLEENLDEVLKYKNIIK
NLQDDNYINNQHSEEEVSPFNFISSKKSLKEYLLEQIMYLDEKEYIKCICNYIIEGIDERGYLAEDIESISKELKVSINL
VIKALNIVQDLEPDGIGARDLIECLRIQCNKREIKDEKIYNIIDNHLEDIAENKYSNIGKKLDISPKKAQMYGDFIKSLQ
PKPSSGFYTGEEVRYIVPDAYIKKIGREYVILMNESITPKLTINNLYKNIINNNEDEAAVEYVKEKLNSASYLIKSIEQR
KSTIYRVLEKIIEIQREYFDKDKKHLKPMTLKEIAEELKVHESTVSRAIREKYIHTDKGIIKIRNLFTTAIESKGNEDSL
SNIAVKNMIEKFIEEEDKKKPLSDQNIADRLKKKDVNISRRTVAKYREEMGIKSSMGRKRY
>Mature_461_residues
MKNVDLGFELSISQEQKLIMTHQMQLSIKILQLSSYELTKLVEKQLQENPLLEINYEEIKKEESLEENLDEVLKYKNIIK
NLQDDNYINNQHSEEEVSPFNFISSKKSLKEYLLEQIMYLDEKEYIKCICNYIIEGIDERGYLAEDIESISKELKVSINL
VIKALNIVQDLEPDGIGARDLIECLRIQCNKREIKDEKIYNIIDNHLEDIAENKYSNIGKKLDISPKKAQMYGDFIKSLQ
PKPSSGFYTGEEVRYIVPDAYIKKIGREYVILMNESITPKLTINNLYKNIINNNEDEAAVEYVKEKLNSASYLIKSIEQR
KSTIYRVLEKIIEIQREYFDKDKKHLKPMTLKEIAEELKVHESTVSRAIREKYIHTDKGIIKIRNLFTTAIESKGNEDSL
SNIAVKNMIEKFIEEEDKKKPLSDQNIADRLKKKDVNISRRTVAKYREEMGIKSSMGRKRY

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=31.6561844863732, Blast_Score=248, Evalue=7e-67,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53979; Mature: 53979

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVDLGFELSISQEQKLIMTHQMQLSIKILQLSSYELTKLVEKQLQENPLLEINYEEIK
CCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCEEECHHHHH
KEESLEENLDEVLKYKNIIKNLQDDNYINNQHSEEEVSPFNFISSKKSLKEYLLEQIMYL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHC
DEKEYIKCICNYIIEGIDERGYLAEDIESISKELKVSINLVIKALNIVQDLEPDGIGARD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LIECLRIQCNKREIKDEKIYNIIDNHLEDIAENKYSNIGKKLDISPKKAQMYGDFIKSLQ
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
PKPSSGFYTGEEVRYIVPDAYIKKIGREYVILMNESITPKLTINNLYKNIINNNEDEAAV
CCCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHCCCCHHHHH
EYVKEKLNSASYLIKSIEQRKSTIYRVLEKIIEIQREYFDKDKKHLKPMTLKEIAEELKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
HESTVSRAIREKYIHTDKGIIKIRNLFTTAIESKGNEDSLSNIAVKNMIEKFIEEEDKKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PLSDQNIADRLKKKDVNISRRTVAKYREEMGIKSSMGRKRY
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCCC
>Mature Secondary Structure
MKNVDLGFELSISQEQKLIMTHQMQLSIKILQLSSYELTKLVEKQLQENPLLEINYEEIK
CCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCEEECHHHHH
KEESLEENLDEVLKYKNIIKNLQDDNYINNQHSEEEVSPFNFISSKKSLKEYLLEQIMYL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHC
DEKEYIKCICNYIIEGIDERGYLAEDIESISKELKVSINLVIKALNIVQDLEPDGIGARD
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LIECLRIQCNKREIKDEKIYNIIDNHLEDIAENKYSNIGKKLDISPKKAQMYGDFIKSLQ
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
PKPSSGFYTGEEVRYIVPDAYIKKIGREYVILMNESITPKLTINNLYKNIINNNEDEAAV
CCCCCCCCCCCCCEEECCHHHHHHCCCCEEEEECCCCCCEEEHHHHHHHHHCCCCHHHHH
EYVKEKLNSASYLIKSIEQRKSTIYRVLEKIIEIQREYFDKDKKHLKPMTLKEIAEELKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
HESTVSRAIREKYIHTDKGIIKIRNLFTTAIESKGNEDSLSNIAVKNMIEKFIEEEDKKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PLSDQNIADRLKKKDVNISRRTVAKYREEMGIKSSMGRKRY
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550525 [H]