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The map label for this gene is ipk

Identifier: 28210046

GI number: 28210046

Start: 267405

End: 268247

Strand: Direct

Name: ipk

Synonym: CTC00283

Alternate gene names: 28210046

Gene position: 267405-268247 (Clockwise)

Preceding gene: 28210045

Following gene: 28210047

Centisome position: 9.55

GC content: 28.35

Gene sequence:

>843_bases
ATGAAAATAAAGGCTTATGCAAAAATCAATCTATCTCTAGATATAGTAGGAGTAAGAGAAGATGGATACCATCTTTTAGA
AATGGTTATGCAAACTATTGATTTATACGATGTAGTACATTTGAATATTAAAGATAAAGATATAGAAATTAATAGTAATA
ACTGTAATATACCTTTAGATAACAGAAACTTAGCATATAAAGCAGCAAAGTTGTTTAAAGAGACTTTTAATATAAAAGAA
GGGGTAGAAATATATATAGAAAAAAATATACCTATAGAAGCAGGGTTAGCAGGTGGAAGTAGTGATGCAGCAGCGGTATT
GAGAGGATTAAGAGATATATTTATGCCTAATTTAAATAATAAAGAACTTGCAAAAATAGGAGTGAGAATAGGTGCAGATG
TACCCTATTGTATTTATGGTGGTACTGCATTTTGTGAAGGAATAGGAGAAAAAATAACTAAATTAAATCCTTTTAAGGAT
CATTATTTGGTGTTAGTTAAACCTGACTTTGGCATATGTACTAAGGAGACTTATAGTAAGATAGACAAAAAAGAAATAAT
AAAACATCCAGATACAAAGGAAATTATAAAATCTATAGGTGAAAATAATTTGAAACTATTGTGTAAAAATATGGAAAATA
TTTTGGAGATAGTAACTTTAGAGGAAAGAAGCAACTTAAAAGATATCAAAAAAGAACTATTACAATATGGTTCTTTAGGA
GCGCAAATGACAGGTAGCGGACCTACTATATTTGGATTTTTCCCATATAGGGATTCGGCAGAAAATTGTTATAGGAAAAT
GAAGAGTAAATATAATGAGGTATATATAAGTAAAACAATATAA

Upstream 100 bases:

>100_bases
GAAGCATTAGCATAAGCCGATACTTGAGATAGAGTTATAAAAAGTATTATACTTAATAAAGAAATAGTAAATACTTTTTT
AGTAAATGTGGAGGTAAAGT

Downstream 100 bases:

>100_bases
TTATATTTTTAAAGCTATGTATATAAAACATGGCTTTTTTATTGTATAAAATACCTGTTAGTTTCAAAAGATATATGAGA
GTTTATATGCGTAAGGGGGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKIKAYAKINLSLDIVGVREDGYHLLEMVMQTIDLYDVVHLNIKDKDIEINSNNCNIPLDNRNLAYKAAKLFKETFNIKE
GVEIYIEKNIPIEAGLAGGSSDAAAVLRGLRDIFMPNLNNKELAKIGVRIGADVPYCIYGGTAFCEGIGEKITKLNPFKD
HYLVLVKPDFGICTKETYSKIDKKEIIKHPDTKEIIKSIGENNLKLLCKNMENILEIVTLEERSNLKDIKKELLQYGSLG
AQMTGSGPTIFGFFPYRDSAENCYRKMKSKYNEVYISKTI

Sequences:

>Translated_280_residues
MKIKAYAKINLSLDIVGVREDGYHLLEMVMQTIDLYDVVHLNIKDKDIEINSNNCNIPLDNRNLAYKAAKLFKETFNIKE
GVEIYIEKNIPIEAGLAGGSSDAAAVLRGLRDIFMPNLNNKELAKIGVRIGADVPYCIYGGTAFCEGIGEKITKLNPFKD
HYLVLVKPDFGICTKETYSKIDKKEIIKHPDTKEIIKSIGENNLKLLCKNMENILEIVTLEERSNLKDIKKELLQYGSLG
AQMTGSGPTIFGFFPYRDSAENCYRKMKSKYNEVYISKTI
>Mature_280_residues
MKIKAYAKINLSLDIVGVREDGYHLLEMVMQTIDLYDVVHLNIKDKDIEINSNNCNIPLDNRNLAYKAAKLFKETFNIKE
GVEIYIEKNIPIEAGLAGGSSDAAAVLRGLRDIFMPNLNNKELAKIGVRIGADVPYCIYGGTAFCEGIGEKITKLNPFKD
HYLVLVKPDFGICTKETYSKIDKKEIIKHPDTKEIIKSIGENNLKLLCKNMENILEIVTLEERSNLKDIKKELLQYGSLG
AQMTGSGPTIFGFFPYRDSAENCYRKMKSKYNEVYISKTI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=274, Percent_Identity=30.6569343065693, Blast_Score=107, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CLOTE (Q899A2)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_780990.1
- ProteinModelPortal:   Q899A2
- SMR:   Q899A2
- GeneID:   1059861
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00283
- NMPDR:   fig|212717.1.peg.212
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- PhylomeDB:   Q899A2
- ProtClustDB:   PRK00128
- BioCyc:   CTET212717:CTC_00283-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR000870
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31617; Mature: 31617

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAYAKINLSLDIVGVREDGYHLLEMVMQTIDLYDVVHLNIKDKDIEINSNNCNIPLD
CCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEEECCEEEECCCCCCCCCC
NRNLAYKAAKLFKETFNIKEGVEIYIEKNIPIEAGLAGGSSDAAAVLRGLRDIFMPNLNN
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCC
KELAKIGVRIGADVPYCIYGGTAFCEGIGEKITKLNPFKDHYLVLVKPDFGICTKETYSK
CHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHH
IDKKEIIKHPDTKEIIKSIGENNLKLLCKNMENILEIVTLEERSNLKDIKKELLQYGSLG
CCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
AQMTGSGPTIFGFFPYRDSAENCYRKMKSKYNEVYISKTI
CEEECCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MKIKAYAKINLSLDIVGVREDGYHLLEMVMQTIDLYDVVHLNIKDKDIEINSNNCNIPLD
CCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEEECCEEEECCCCCCCCCC
NRNLAYKAAKLFKETFNIKEGVEIYIEKNIPIEAGLAGGSSDAAAVLRGLRDIFMPNLNN
CCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCC
KELAKIGVRIGADVPYCIYGGTAFCEGIGEKITKLNPFKDHYLVLVKPDFGICTKETYSK
CHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHH
IDKKEIIKHPDTKEIIKSIGENNLKLLCKNMENILEIVTLEERSNLKDIKKELLQYGSLG
CCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
AQMTGSGPTIFGFFPYRDSAENCYRKMKSKYNEVYISKTI
CEEECCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129