Click here to switch to the map view.

The map label for this gene is metG

Identifier: 28210009

GI number: 28210009

Start: 211425

End: 213350

Strand: Direct

Name: metG

Synonym: CTC00243

Alternate gene names: 28210009

Gene position: 211425-213350 (Clockwise)

Preceding gene: 28210008

Following gene: 28210010

Centisome position: 7.55

GC content: 30.84

Gene sequence:

>1926_bases
ATGAATAAAAAAACTTTTTACATTACTACACCAATATACTATCCATCAGCTAAATTGCATATTGGGAATACTTATACTAC
AGTTGCAGCAGATGCATTAGCTAGGTTTAAACGATTAACTGGGCATGATGTATTATTCTTAACTGGAACAGATGAACATG
GGCAAAAGATTCAGAGGGTAGCGGAAGAAAAGGGATTAAAACCTAAAGAGTATTTAGATAATATGGTTGACAGCATAAAA
GAATTATGGAAATCAATGAATATAAGCTACGATAAATTTATAAGAACTACAGATGATTATCATATAGAATCTGTACAAAA
AATATTCAAAAAGTTATACGAACAAGGAGATATATACAAGGGTGAATATGAAGGATGGTATTGTACACCTTGTGAATCTT
TCTGGACAGAATCACAACTAGATGATCACAATTGTCCTGATTGTGGAAGGCCTGTAGAAAAGACAAAGGAAGAAGCATAT
TTCTTCAAGATGTCTAAATATGCAGATAGACTAATTAAATATATAGAAGAAAATCCCCATTTTATACAACCAGAATCAAG
AAAAAATGAAATGTTAAATAATTTTTTAAAACCAGGGTTGCAAGATTTATGTATTTCAAGAACCTCATTTGACTGGGGTA
TACCTGTAAGCTTTGATAATAAGCATGTTATATATGTTTGGATAGATGCTTTATCTAACTATATAACAGCATTAGGATAC
AATAGTGATAATCAAGAACTATTAGAAAAGTTTTGGCCAGCAAATGTACATTTAGTTGGAAAAGATATATTAAGATTCCA
TACAATATATTGGCCTATTATGCTTATGGCATTGGGAATTGAACTACCAAAGCAAGTATTTGGTCATGGATGGCTTTTAG
TTGATGGTGGAAAAATGTCTAAATCAAAAGGGAATGTTGTGGATCCAGTTGTTTTAGTAGATCATTTTGGTGAAGATACA
GTTAGATATTATCTTTTAAGAGAAATCCCATTTGGATCAGATGGATTATTTAATAACGAATTATTCATAAAGAAAATAAA
TTCTGATTTAGCAAATGACTTAGGAAACTTACTTTCAAGAACTGTAGCTATGGTACAAAAATATTTCAACGGGATAATGC
CAGCACCTATAGCCAAAGAACCTATTGACGATGAACTTATAAATTTAGCTTTAGATACTAGAGAAAAAGTTGAAAATAAT
ATGGAAAAATTAAAAATACCAGAAGTATTAGATGAGATTTGGACACTTATAGGAAGAGCAAATAAATATATAGATGAAAC
GACTCCTTGGATACTTGCTAAAGATGAAGATAAAAAGGACAGACTAGGAACTGTATTATATAATCTATCAGAAACTTTGA
GAATCATTTCAGTTCTTATATCTGCATTCATACCAAAGACTAGCGAAAGAATAAATGAACAATTAAATGTAGATTTAACT
ACTTGGGATAGTATAGCTTCCTTTGATGGCACTAAGGCTGGTACAAAAGTAGTAAAAGGTGATGCATTATTCCCAAGAAT
AGATGTAGAAGCTAAAATTGAAGAACTTAATAGTCTAAAAGAGAAGAAGGAAAAGAAAGAAATTAAACCTATAAAAGAAG
AAATAACTATAGACGATTTTGATAAAATTGACTTAAGAGTAGTAAAGGTAATTTCTTGTGAGCCAGTAAAAGGTGCAAAG
AAACTTTTAAAATTAAAAGTGGATTTAGGTGGAGAAGAAAGACAAGTAATTTCAGGAATTGCTCAATATTATAAACCAGA
AGAACTAGTAGGTAAGTCTGTGGTTTTAGTTGCAAATTTAAAGCCAGCAAAATTAAGAGGAGAATTATCCCAAGGAATGA
TATTAGCAGCTGCTACAGATGATGATAGTAAGTTATTTACTGTGAGTATTCCAGGTGAACTACCAACAGGAAGTCAAGTT
AGATAA

Upstream 100 bases:

>100_bases
CGCGTTTTGAGCAATACGCCTCTATACGGAATATTTCGTATAGAGGCTTTTTTGTTTATACAATTAAATTTTAGAAAATT
AATTATAGGAGGAGTTTAAT

Downstream 100 bases:

>100_bases
TATCATAAATAGCTTATAAGTAATAGCTTATAAGCTATTTTCTTCACGGAAATATATTTTTAATATAATATGAATAAAAA
ATAATTATATGGTAATATTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MNKKTFYITTPIYYPSAKLHIGNTYTTVAADALARFKRLTGHDVLFLTGTDEHGQKIQRVAEEKGLKPKEYLDNMVDSIK
ELWKSMNISYDKFIRTTDDYHIESVQKIFKKLYEQGDIYKGEYEGWYCTPCESFWTESQLDDHNCPDCGRPVEKTKEEAY
FFKMSKYADRLIKYIEENPHFIQPESRKNEMLNNFLKPGLQDLCISRTSFDWGIPVSFDNKHVIYVWIDALSNYITALGY
NSDNQELLEKFWPANVHLVGKDILRFHTIYWPIMLMALGIELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVDHFGEDT
VRYYLLREIPFGSDGLFNNELFIKKINSDLANDLGNLLSRTVAMVQKYFNGIMPAPIAKEPIDDELINLALDTREKVENN
MEKLKIPEVLDEIWTLIGRANKYIDETTPWILAKDEDKKDRLGTVLYNLSETLRIISVLISAFIPKTSERINEQLNVDLT
TWDSIASFDGTKAGTKVVKGDALFPRIDVEAKIEELNSLKEKKEKKEIKPIKEEITIDDFDKIDLRVVKVISCEPVKGAK
KLLKLKVDLGGEERQVISGIAQYYKPEELVGKSVVLVANLKPAKLRGELSQGMILAAATDDDSKLFTVSIPGELPTGSQV
R

Sequences:

>Translated_641_residues
MNKKTFYITTPIYYPSAKLHIGNTYTTVAADALARFKRLTGHDVLFLTGTDEHGQKIQRVAEEKGLKPKEYLDNMVDSIK
ELWKSMNISYDKFIRTTDDYHIESVQKIFKKLYEQGDIYKGEYEGWYCTPCESFWTESQLDDHNCPDCGRPVEKTKEEAY
FFKMSKYADRLIKYIEENPHFIQPESRKNEMLNNFLKPGLQDLCISRTSFDWGIPVSFDNKHVIYVWIDALSNYITALGY
NSDNQELLEKFWPANVHLVGKDILRFHTIYWPIMLMALGIELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVDHFGEDT
VRYYLLREIPFGSDGLFNNELFIKKINSDLANDLGNLLSRTVAMVQKYFNGIMPAPIAKEPIDDELINLALDTREKVENN
MEKLKIPEVLDEIWTLIGRANKYIDETTPWILAKDEDKKDRLGTVLYNLSETLRIISVLISAFIPKTSERINEQLNVDLT
TWDSIASFDGTKAGTKVVKGDALFPRIDVEAKIEELNSLKEKKEKKEIKPIKEEITIDDFDKIDLRVVKVISCEPVKGAK
KLLKLKVDLGGEERQVISGIAQYYKPEELVGKSVVLVANLKPAKLRGELSQGMILAAATDDDSKLFTVSIPGELPTGSQV
R
>Mature_641_residues
MNKKTFYITTPIYYPSAKLHIGNTYTTVAADALARFKRLTGHDVLFLTGTDEHGQKIQRVAEEKGLKPKEYLDNMVDSIK
ELWKSMNISYDKFIRTTDDYHIESVQKIFKKLYEQGDIYKGEYEGWYCTPCESFWTESQLDDHNCPDCGRPVEKTKEEAY
FFKMSKYADRLIKYIEENPHFIQPESRKNEMLNNFLKPGLQDLCISRTSFDWGIPVSFDNKHVIYVWIDALSNYITALGY
NSDNQELLEKFWPANVHLVGKDILRFHTIYWPIMLMALGIELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVDHFGEDT
VRYYLLREIPFGSDGLFNNELFIKKINSDLANDLGNLLSRTVAMVQKYFNGIMPAPIAKEPIDDELINLALDTREKVENN
MEKLKIPEVLDEIWTLIGRANKYIDETTPWILAKDEDKKDRLGTVLYNLSETLRIISVLISAFIPKTSERINEQLNVDLT
TWDSIASFDGTKAGTKVVKGDALFPRIDVEAKIEELNSLKEKKEKKEIKPIKEEITIDDFDKIDLRVVKVISCEPVKGAK
KLLKLKVDLGGEERQVISGIAQYYKPEELVGKSVVLVANLKPAKLRGELSQGMILAAATDDDSKLFTVSIPGELPTGSQV
R

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI24308436, Length=543, Percent_Identity=34.622467771639, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI14043022, Length=522, Percent_Identity=28.1609195402299, Blast_Score=177, Evalue=4e-44,
Organism=Homo sapiens, GI7661872, Length=183, Percent_Identity=26.2295081967213, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788432, Length=689, Percent_Identity=29.8984034833091, Blast_Score=303, Evalue=3e-83,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=37.037037037037, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786861, Length=194, Percent_Identity=25.7731958762887, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI212645096, Length=523, Percent_Identity=32.6959847036329, Blast_Score=275, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=411, Percent_Identity=31.3868613138686, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17540794, Length=565, Percent_Identity=26.0176991150442, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71997510, Length=168, Percent_Identity=31.547619047619, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71997517, Length=168, Percent_Identity=31.547619047619, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321610, Length=523, Percent_Identity=33.6520076481836, Blast_Score=289, Evalue=9e-79,
Organism=Saccharomyces cerevisiae, GI6321704, Length=562, Percent_Identity=26.6903914590747, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323414, Length=180, Percent_Identity=28.8888888888889, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21358483, Length=556, Percent_Identity=33.9928057553957, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24655311, Length=560, Percent_Identity=26.4285714285714, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355409, Length=175, Percent_Identity=26.2857142857143, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357223, Length=100, Percent_Identity=35, Blast_Score=66, Evalue=1e-10,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CLOTE (Q899D9)

Other databases:

- EMBL:   AE015927
- RefSeq:   NP_780953.1
- ProteinModelPortal:   Q899D9
- SMR:   Q899D9
- GeneID:   1059813
- GenomeReviews:   AE015927_GR
- KEGG:   ctc:CTC00243
- NMPDR:   fig|212717.1.peg.175
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- PhylomeDB:   Q899D9
- ProtClustDB:   PRK12267
- BioCyc:   CTET212717:CTC_00243-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 73212; Mature: 73212

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTFYITTPIYYPSAKLHIGNTYTTVAADALARFKRLTGHDVLFLTGTDEHGQKIQRV
CCCCEEEEECCEEECCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
AEEKGLKPKEYLDNMVDSIKELWKSMNISYDKFIRTTDDYHIESVQKIFKKLYEQGDIYK
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
GEYEGWYCTPCESFWTESQLDDHNCPDCGRPVEKTKEEAYFFKMSKYADRLIKYIEENPH
CCCCCEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIQPESRKNEMLNNFLKPGLQDLCISRTSFDWGIPVSFDNKHVIYVWIDALSNYITALGY
EECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHCC
NSDNQELLEKFWPANVHLVGKDILRFHTIYWPIMLMALGIELPKQVFGHGWLLVDGGKMS
CCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCC
KSKGNVVDPVVLVDHFGEDTVRYYLLREIPFGSDGLFNNELFIKKINSDLANDLGNLLSR
CCCCCCCCHHHEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHH
TVAMVQKYFNGIMPAPIAKEPIDDELINLALDTREKVENNMEKLKIPEVLDEIWTLIGRA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NKYIDETTPWILAKDEDKKDRLGTVLYNLSETLRIISVLISAFIPKTSERINEQLNVDLT
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEE
TWDSIASFDGTKAGTKVVKGDALFPRIDVEAKIEELNSLKEKKEKKEIKPIKEEITIDDF
ECHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
DKIDLRVVKVISCEPVKGAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKSVVLVANL
CCCCEEEEEEEECCCCHHHHHHHEEEECCCCCHHHHHHHHHHHCCCHHHCCCCEEEEECC
KPAKLRGELSQGMILAAATDDDSKLFTVSIPGELPTGSQVR
CCHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNKKTFYITTPIYYPSAKLHIGNTYTTVAADALARFKRLTGHDVLFLTGTDEHGQKIQRV
CCCCEEEEECCEEECCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
AEEKGLKPKEYLDNMVDSIKELWKSMNISYDKFIRTTDDYHIESVQKIFKKLYEQGDIYK
HHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
GEYEGWYCTPCESFWTESQLDDHNCPDCGRPVEKTKEEAYFFKMSKYADRLIKYIEENPH
CCCCCEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FIQPESRKNEMLNNFLKPGLQDLCISRTSFDWGIPVSFDNKHVIYVWIDALSNYITALGY
EECCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEECCCEEEEEEHHHHHHHHHHHCC
NSDNQELLEKFWPANVHLVGKDILRFHTIYWPIMLMALGIELPKQVFGHGWLLVDGGKMS
CCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCC
KSKGNVVDPVVLVDHFGEDTVRYYLLREIPFGSDGLFNNELFIKKINSDLANDLGNLLSR
CCCCCCCCHHHEEECCCCHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHH
TVAMVQKYFNGIMPAPIAKEPIDDELINLALDTREKVENNMEKLKIPEVLDEIWTLIGRA
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NKYIDETTPWILAKDEDKKDRLGTVLYNLSETLRIISVLISAFIPKTSERINEQLNVDLT
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCEEEE
TWDSIASFDGTKAGTKVVKGDALFPRIDVEAKIEELNSLKEKKEKKEIKPIKEEITIDDF
ECHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
DKIDLRVVKVISCEPVKGAKKLLKLKVDLGGEERQVISGIAQYYKPEELVGKSVVLVANL
CCCCEEEEEEEECCCCHHHHHHHEEEECCCCCHHHHHHHHHHHCCCHHHCCCCEEEEECC
KPAKLRGELSQGMILAAATDDDSKLFTVSIPGELPTGSQVR
CCHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129