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The map label for this gene is hsdS [H]

Identifier: 28199935

GI number: 28199935

Start: 2460649

End: 2461866

Strand: Reverse

Name: hsdS [H]

Synonym: PD2075

Alternate gene names: 28199935

Gene position: 2461866-2460649 (Counterclockwise)

Preceding gene: 28199936

Following gene: 77747723

Centisome position: 97.7

GC content: 49.01

Gene sequence:

>1218_bases
ATGAGTCGGATTGATGAGTTGATTGCAGCGCTTTGTCCGGAGGGGGTGGGGTTCATGAGAGTTGGTGAGTTGCTTGAAAG
AACCTCAAATATCCGATGGCAGGATACTCAGGGTGAAGAATTCCAATATATCGATCTGTCTTCGGTTGATCGCAATACCC
ACATTATTAGAGGCACAAAAACCATTAATAGTGGAACCGCGCCGAGTCGGGCGCAGCAAATCGTGCGCGAAAACGATGTA
ATTTTTGGTACAACAAGGCCGATGCTCAAACGCTACTGCCTCATTCCTTCTGAATACGATGGGCAAATATCTAGCACTGG
TTACTGTGTCTTTCGACCAAAAAATGAATTGCTTCTACCAAATTTTCTGTTTCATCTACTAGGCACAAAAGCCTTCTACA
GCTATGTAGAAGCAAATCAGAATGGTGCAAGCTATCCTGTCATCACGGACGAGGCGGTAAAAGCATTTCGCATTCCTAGA
CTGCCGGTAGAAGTTCAGGCTGAAATTGCAAAAGTACTTGATACCTTCACCACGCTAGAGGCGGAGCTGGAGGCGGAGCT
GGAGACTCGTCGGCGGCAGTATCAGTACTACCGGGATGCGCTTTTGACGTTCGGGGAGGGTACGGATGCTGCAACAAGGG
TCAGGTGGGTGACTTTGGGGGAGATTGCAACTTACGCTAATACGCGAATTCAGTCCGTCGGATTAGATGCATCTTCGTAT
GTTGGGGTTGACAATCTGCTTCCTGACACACGAGGTAAGGTTCGCTCAAATTTTGTCCCGACATCTGGAACTGTCATCGG
TTATCAGGCCAATGACATCCTGATAGGAAATATTCGCCCCTACCTCAAGAAGATATGGCTTGCGCATTCAACAGGTGGTA
CCAATCAAGATGTGCTTGTTATTCGTATCAAGGATGAAGCCAAGGCCATGCTCAAGCCTCGCTATCTTTACTATTTGCTG
GCTTCGGATGATTTCTTCACATATGACTCGCAACATGCAAAGGGCGCCAAGATGCCTCGTGGTGATAAGACGATGATCAT
GAAATATAAGATCCCCATCCCCCCCCTCGAAGTCCAAGCCCGCATCGTTGCGGTTCTCGATCAATTCGACACACTGGTCA
ATGACATCACGGCCGGTTTGCCTGCCGAGATTGCGGCACGCCGCCAGCAGTACGCGTACTACCGCGACCGGTTGCTCACG
TTCAAGGAGGCTGTATGA

Upstream 100 bases:

>100_bases
AAGACGCTACGCAACGCTGCGGCCAGCAGCTTGATGTGGTAGCAGTCTCTCTAACAATGGTGAAAGTAATGCAGCAAAAA
AATAGCGGAAAAAAACGGGG

Downstream 100 bases:

>100_bases
GTGAACCTATCCCACCCTTTCGTTATGCACCTATTGTGTGCTCTGAGCAGAGCACCATTGTTGCCGAGTTTGTACCCGAG
GCCTCGGGTGTGCGTGAGGC

Product: type I restriction-modification system specificity determinant

Products: NA

Alternate protein names: S.EcoR124II; Type I restriction enzyme EcoR124II specificity protein; S protein [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSRIDELIAALCPEGVGFMRVGELLERTSNIRWQDTQGEEFQYIDLSSVDRNTHIIRGTKTINSGTAPSRAQQIVRENDV
IFGTTRPMLKRYCLIPSEYDGQISSTGYCVFRPKNELLLPNFLFHLLGTKAFYSYVEANQNGASYPVITDEAVKAFRIPR
LPVEVQAEIAKVLDTFTTLEAELEAELETRRRQYQYYRDALLTFGEGTDAATRVRWVTLGEIATYANTRIQSVGLDASSY
VGVDNLLPDTRGKVRSNFVPTSGTVIGYQANDILIGNIRPYLKKIWLAHSTGGTNQDVLVIRIKDEAKAMLKPRYLYYLL
ASDDFFTYDSQHAKGAKMPRGDKTMIMKYKIPIPPLEVQARIVAVLDQFDTLVNDITAGLPAEIAARRQQYAYYRDRLLT
FKEAV

Sequences:

>Translated_405_residues
MSRIDELIAALCPEGVGFMRVGELLERTSNIRWQDTQGEEFQYIDLSSVDRNTHIIRGTKTINSGTAPSRAQQIVRENDV
IFGTTRPMLKRYCLIPSEYDGQISSTGYCVFRPKNELLLPNFLFHLLGTKAFYSYVEANQNGASYPVITDEAVKAFRIPR
LPVEVQAEIAKVLDTFTTLEAELEAELETRRRQYQYYRDALLTFGEGTDAATRVRWVTLGEIATYANTRIQSVGLDASSY
VGVDNLLPDTRGKVRSNFVPTSGTVIGYQANDILIGNIRPYLKKIWLAHSTGGTNQDVLVIRIKDEAKAMLKPRYLYYLL
ASDDFFTYDSQHAKGAKMPRGDKTMIMKYKIPIPPLEVQARIVAVLDQFDTLVNDITAGLPAEIAARRQQYAYYRDRLLT
FKEAV
>Mature_404_residues
SRIDELIAALCPEGVGFMRVGELLERTSNIRWQDTQGEEFQYIDLSSVDRNTHIIRGTKTINSGTAPSRAQQIVRENDVI
FGTTRPMLKRYCLIPSEYDGQISSTGYCVFRPKNELLLPNFLFHLLGTKAFYSYVEANQNGASYPVITDEAVKAFRIPRL
PVEVQAEIAKVLDTFTTLEAELEAELETRRRQYQYYRDALLTFGEGTDAATRVRWVTLGEIATYANTRIQSVGLDASSYV
GVDNLLPDTRGKVRSNFVPTSGTVIGYQANDILIGNIRPYLKKIWLAHSTGGTNQDVLVIRIKDEAKAMLKPRYLYYLLA
SDDFFTYDSQHAKGAKMPRGDKTMIMKYKIPIPPLEVQARIVAVLDQFDTLVNDITAGLPAEIAARRQQYAYYRDRLLTF
KEAV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 45817; Mature: 45686

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIDELIAALCPEGVGFMRVGELLERTSNIRWQDTQGEEFQYIDLSSVDRNTHIIRGTK
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCEEEEECE
TINSGTAPSRAQQIVRENDVIFGTTRPMLKRYCLIPSEYDGQISSTGYCVFRPKNELLLP
ECCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEHH
NFLFHLLGTKAFYSYVEANQNGASYPVITDEAVKAFRIPRLPVEVQAEIAKVLDTFTTLE
HHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AELEAELETRRRQYQYYRDALLTFGEGTDAATRVRWVTLGEIATYANTRIQSVGLDASSY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEHHHHHHHHCCEEEEECCCCHHC
VGVDNLLPDTRGKVRSNFVPTSGTVIGYQANDILIGNIRPYLKKIWLAHSTGGTNQDVLV
CCHHHCCCCCCCCHHCCCCCCCCEEEEEECCCEEEECCHHHHHHHHHEECCCCCCCCEEE
IRIKDEAKAMLKPRYLYYLLASDDFFTYDSQHAKGAKMPRGDKTMIMKYKIPIPPLEVQA
EEECCCHHHHCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
RIVAVLDQFDTLVNDITAGLPAEIAARRQQYAYYRDRLLTFKEAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIDELIAALCPEGVGFMRVGELLERTSNIRWQDTQGEEFQYIDLSSVDRNTHIIRGTK
CHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCEEEEECE
TINSGTAPSRAQQIVRENDVIFGTTRPMLKRYCLIPSEYDGQISSTGYCVFRPKNELLLP
ECCCCCCHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEHH
NFLFHLLGTKAFYSYVEANQNGASYPVITDEAVKAFRIPRLPVEVQAEIAKVLDTFTTLE
HHHHHHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
AELEAELETRRRQYQYYRDALLTFGEGTDAATRVRWVTLGEIATYANTRIQSVGLDASSY
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEEEHHHHHHHHCCEEEEECCCCHHC
VGVDNLLPDTRGKVRSNFVPTSGTVIGYQANDILIGNIRPYLKKIWLAHSTGGTNQDVLV
CCHHHCCCCCCCCHHCCCCCCCCEEEEEECCCEEEECCHHHHHHHHHEECCCCCCCCEEE
IRIKDEAKAMLKPRYLYYLLASDDFFTYDSQHAKGAKMPRGDKTMIMKYKIPIPPLEVQA
EEECCCHHHHCCCEEEEEEEECCCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHH
RIVAVLDQFDTLVNDITAGLPAEIAARRQQYAYYRDRLLTFKEAV
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]