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The map label for this gene is rpoH [H]

Identifier: 28199909

GI number: 28199909

Start: 2414998

End: 2415879

Strand: Reverse

Name: rpoH [H]

Synonym: PD2048

Alternate gene names: 28199909

Gene position: 2415879-2414998 (Counterclockwise)

Preceding gene: 28199910

Following gene: 28199907

Centisome position: 95.88

GC content: 47.17

Gene sequence:

>882_bases
TTGGGATACTTGAGTATGACTCGAAATACATCAATGGTGCTTGTGGGAAACAACTTTCCTGTTCCAAATGCCCTCGGTTC
GTTGGAGGCATACATCAGTGCCGTGCACCAGATATCGGTACTTTCGGTTGATGAGGAGCAGCTATTGGCTCGCCGTTATC
GTGATCAACAGGATTTAGATGCTGCACGTGAGTTGGTGCATTCGCACTTGCGTTTTGTTGTTCGGGTAGCGCGCGGTTAT
AGCGGTTACGGCCTGTCTTTAGGCGACTTAATACAAGAGGGTAATATCGGTCTGATGAAAGCTGTAAAGCGCTTTGATCC
GGATATGGGTGTCCGTTTGGTTTCTTTTGCAATGCATTGGATTCGCGCTGAAATGCATGAGTTTATTTTGAAGAATTGGC
GCATCGTTAAAGTTGCTACCACCAAAGCGCAGCGAAAGCTGTTTTTTAACCTTCGGAAATCTAGAACCCGTCTGGGCTGG
ATGAATGCCGAGGAAGTTAGTGCAGTTGCTAGGGATCTTAACGTCTCCGAACGTGAGGTATTGGAGATGGAGTCACGTTT
GTCAGGGCGCGATATTGGTTTCGACGCGCCGGAGGAAGACAACGCTCCGCCATCGCCTGTTGGCTACTTGATTGCTAATG
ATGGAGATCCTTCTCAAGCTTACGAACGTGGTAATAGTGAGGAAACCCAATTACGCCTATTGCAGGAAGGTTTGTCTGTC
CTTGATACCCGTTCGCGCGATATTATTCAGCGTCGCTGGCTGGAAACAGATAAGAAAATCACGTTGCAAGAATTAGCTGA
AGAATATGGAGTTTCAGCAGAACGTATTCGGCAGATTGAAGCTAATGCGCTTAAAAAAATGAAGGTGCTGTTCGCTGCTT
GA

Upstream 100 bases:

>100_bases
CCCATGACAATGTTAGGATGCCATTTTAATTTAAATGGAGTTTTTCTAAAGATTTGTTCATTTTAAACGTATAGCTGTCA
CTGTGTGGTTACTTTAAGAT

Downstream 100 bases:

>100_bases
ATGCTCTGTTTCAAAACAAAGGCTACAAAGTGGCTAGTGGTTGTTGCTTGTATGTATTTTTCTCGTTTTTGAAGATGCTT
GGATCTTCCTGATGCTTAGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MGYLSMTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAARELVHSHLRFVVRVARGY
SGYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGW
MNAEEVSAVARDLNVSEREVLEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSV
LDTRSRDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA

Sequences:

>Translated_293_residues
MGYLSMTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAARELVHSHLRFVVRVARGY
SGYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGW
MNAEEVSAVARDLNVSEREVLEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSV
LDTRSRDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA
>Mature_292_residues
GYLSMTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAARELVHSHLRFVVRVARGYS
GYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWM
NAEEVSAVARDLNVSEREVLEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSVL
DTRSRDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=55.5147058823529, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1789098, Length=270, Percent_Identity=27.7777777777778, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=30.7692307692308, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33311; Mature: 33179

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYLSMTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLD
CCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
AARELVHSHLRFVVRVARGYSGYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHW
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEEVSAVARDLNVSEREV
HHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
LEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSV
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHH
LDTRSRDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA
HHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GYLSMTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLD
CCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
AARELVHSHLRFVVRVARGYSGYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHW
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEEVSAVARDLNVSEREV
HHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHH
LEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSV
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHH
LDTRSRDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA
HHCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]