Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 28199739

GI number: 28199739

Start: 2198000

End: 2199487

Strand: Reverse

Name: murE [H]

Synonym: PD1870

Alternate gene names: 28199739

Gene position: 2199487-2198000 (Counterclockwise)

Preceding gene: 28199740

Following gene: 28199738

Centisome position: 87.29

GC content: 56.25

Gene sequence:

>1488_bases
ATGAGGCGTTCTATGCCATTGGCGCAGCTATTGCCCGATATTCCGCAAGCGCGTGATGTCGTGATCTCTGGTTTGGTGAT
GGATAGCCGTGAGGTGCAGCCGGGTGATGCGTTTGTCGCGGTTGCAGGGTTCGGCGTTCACGGGTTGTGTTTTATTGAGG
ATGCGTGTGCCAGAGGTGCTGTTGCAATTTTGTTTGAGCCGCCCGCACCGCAGGGGGTGTCAGTGCCGGATGGTGCGATT
GCAGTGCATGGTTTACGTGCGCGTTTGGGTGCGATGGCGGATCGTTTCCATGGCCATCCGTCGCAGGCGATGACCATGGT
GGGGGTCACTGGCACCAATGGTAAGACGTCCACTGTGCAGTTGTTGGCCCAGGCTTGGCATCGTCTGGGTGTGCGCAGTG
CGACCTGTGGAACGCTCGGTGTGGGTTTGTACGATCAGCTGGTACCGACGGGTTTCACGACGCCGCTGGTATTGCAATTG
CATCAGTGTCTGGGACAACTGCGTGATGATGGTGCGCAGGCGGTGGCGATGGAAGTCAGTTCCCACGCTCTTGACCAGGG
ACGCGTGGATGGCGTGCATTATGACGTTGCGGTGTTCACCAATCTGACGCGCGATCATCTTGATTATCACGGTGACATGG
AGCACTACGGTGAGGCTAAGGCTCGCTTGTTCGCGCATCAGGATGTGCAGGCGGCGGTGATCAATGTGGATGATCTTTTC
GGTTTGCGTCTGTTACATGGTTTGGCCAATGGGGTGCGGCGGGTTGGAGTCAGCGTGTGTGGTCACACGGATGCAGATGT
GATGGCGCAGCATCTGAGTCTGAATCTTCAGGGTATCGGGTTTGATCTTGTCATCGGTGCGGATCGTGCGCCGGTGCGTT
CACCTCTGATGGGACGTTTCAACGTTGATAATTTGCTTACGGTAGCAGGTGTTCTTTATGCGCTGGATTACGCGCTGTCT
GAGATTGCTGCGGTGCTGTCGACGTTGCGGCCGATTCATGGCCGGATGAATCGTTTGGGTGGTCAGGATGGGCAGCCTGT
GGTGGTGGTTGATTACGCGCATACCCCTGATGCGCTGGGGCAGGTGTTGTCCAGTTTGAGTAGCCACGTATGCGGTCGTT
TGATTTGTGTGTTTGGCTGTGGCGGTGAGCGCGATAGGGGTAAACGTTCACAGATGGCTGTTATTGCCGAGAGTAACGCC
GATGTTGTGTTGGTGACCGATGATAATCCGCGTGGTGAGGATGGTGATGGCATTGTTGCTGACATTCTGGCCGGTTTTGT
GCGTCCCAACCTCGTAAAAGTGCAGCGTGACCGTTCTGCTGCGATTGCGGCAGCCATTGGTATAGCCAGTGCAGGGGATG
TTGTGTTGATTGCTGGCAAAGGTCACGAGCGATATCAGGAGGTTGCTGGGGTGCGTCATCCGTTCGATGACACTGAGGTT
GCTATGCGTGTGCTGGCAGCAATGTCGGCCCAGGAGACTGTCCGATGA

Upstream 100 bases:

>100_bases
ATCCCGATCCGGCATTAGTACCCGATGCTGCCGATGAGGTCGCCGCTGGTATTCCTACGACGGTGACACCATCGCCGTCT
GCGATTCATAGAGATCGCCA

Downstream 100 bases:

>100_bases
GATCTATGCCACTTTCGTTAATTGCGTACTGGACCGGTGGTTATCTGCACGGTGAGGATGTCAGGGTGTGTGCAATTGGT
CATGACACCCGTGCGCTGGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRRSMPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAILFEPPAPQGVSVPDGAI
AVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQL
HQCLGQLRDDGAQAVAMEVSSHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLF
GLRLLHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDNLLTVAGVLYALDYALS
EIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNA
DVVLVTDDNPRGEDGDGIVADILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEV
AMRVLAAMSAQETVR

Sequences:

>Translated_495_residues
MRRSMPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAILFEPPAPQGVSVPDGAI
AVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQL
HQCLGQLRDDGAQAVAMEVSSHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLF
GLRLLHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDNLLTVAGVLYALDYALS
EIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNA
DVVLVTDDNPRGEDGDGIVADILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEV
AMRVLAAMSAQETVR
>Mature_495_residues
MRRSMPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGAVAILFEPPAPQGVSVPDGAI
AVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQLLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQL
HQCLGQLRDDGAQAVAMEVSSHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLF
GLRLLHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRFNVDNLLTVAGVLYALDYALS
EIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALGQVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNA
DVVLVTDDNPRGEDGDGIVADILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEV
AMRVLAAMSAQETVR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=494, Percent_Identity=42.914979757085, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52396; Mature: 52396

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSMPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGA
CCCCCCHHHHCCCCCCHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCE
VAILFEPPAPQGVSVPDGAIAVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQ
EEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
LLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCLGQLRDDGAQAVAMEVS
HHHHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHHH
SHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLF
HHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHH
GLRLLHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRF
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCEEEEECCCCCCCCCCCCCCC
NVDNLLTVAGVLYALDYALSEIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
QVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVLVTDDNPRGEDGDGIVA
HHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHH
DILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEV
HHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHH
AMRVLAAMSAQETVR
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRSMPLAQLLPDIPQARDVVISGLVMDSREVQPGDAFVAVAGFGVHGLCFIEDACARGA
CCCCCCHHHHCCCCCCHHHHHHHHEEECCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCE
VAILFEPPAPQGVSVPDGAIAVHGLRARLGAMADRFHGHPSQAMTMVGVTGTNGKTSTVQ
EEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHH
LLAQAWHRLGVRSATCGTLGVGLYDQLVPTGFTTPLVLQLHQCLGQLRDDGAQAVAMEVS
HHHHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHEEEHHHH
SHALDQGRVDGVHYDVAVFTNLTRDHLDYHGDMEHYGEAKARLFAHQDVQAAVINVDDLF
HHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHH
GLRLLHGLANGVRRVGVSVCGHTDADVMAQHLSLNLQGIGFDLVIGADRAPVRSPLMGRF
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCEEEEECCCCCCCCCCCCCCC
NVDNLLTVAGVLYALDYALSEIAAVLSTLRPIHGRMNRLGGQDGQPVVVVDYAHTPDALG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
QVLSSLSSHVCGRLICVFGCGGERDRGKRSQMAVIAESNADVVLVTDDNPRGEDGDGIVA
HHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCHHH
DILAGFVRPNLVKVQRDRSAAIAAAIGIASAGDVVLIAGKGHERYQEVAGVRHPFDDTEV
HHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHH
AMRVLAAMSAQETVR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]