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The map label for this gene is mesJ [H]

Identifier: 28199394

GI number: 28199394

Start: 1758944

End: 1760257

Strand: Reverse

Name: mesJ [H]

Synonym: PD1514

Alternate gene names: 28199394

Gene position: 1760257-1758944 (Counterclockwise)

Preceding gene: 77747701

Following gene: 28199393

Centisome position: 69.86

GC content: 62.48

Gene sequence:

>1314_bases
TTGACCGTGTCTTCTCCCTTTCCCCGCATTCCAGATCCTGGCGTTCCCGTCCTGGTTGCTTTCAGCGGCGGATTGGATTC
AACCGTCCTGCTGCATTGCTTAGCCAGCCAGCCAACCCAGCGCATCCACGGCCTGGAAGCCATCCACATCCATCATGGAC
TCAATGAAAACGCCGATGCTTGGACCGCCCACTGCGCCGCCTTTTGCACACAACACGACATCCGCCTACACATCGCGCGT
GTCCACGTTTCCCACAACAGCGGACAAGGCTTGGAAGCGGCCGCCCGCACCGCGCGCCGCGCCGCATTTGCCCACACCTT
GCAACATGGCCACTACCTGGCATTGGCGCATCATTGCGATGACCAAGCCGAGACATGGTTGTTACGCGCACTACGTGGCT
CCTGCGACGGACTCGCCGCCATGCGGCCATTGACCCCATTTGCCGCGGGCCACCTGTGGCGTCCCCTGCTGACCCACTCC
CGGGCGCAATTATTGGACTACGCACAACAGCAGCACCTGGATTGGATTGAAGATTCCAGTAACGCGGATCTGCGTCATGA
CCGCAACTTCCTGCGCATCCACGTATTGCCATTACTGCATCAGCGCTGGCCCCAGGCCACGGCTGTACTGGCCCGCAATG
CGGCACTGGCAGCGGCCAACGCCGACCTCCTCAATGCTGAAGATGCCGTACTGCTGCCTGACCTACTTGACCCTGACGGC
GCATTGGACATCAACGCCCTCACTGCCCACCCACCCGCACGGCGCGCCCGACTGCTGCGCGCCTGGTGTGCCCGCGCAGG
TGCTCCCCCACTCCCTGAGCGCGGTGTGAACATTATCGAGCGCGAACTACTGCCTGCCAGACACGATTCCGCCGCCTGCT
TCACCTGGTCTCATACCGAGATCCGGCGCTGGCGCCTGCGCCTATACCTGCATCGTCCACAACCGCCATGGCCCCCTGAC
TGGCAGCCCCTGTGGAGCGGCACCGCACCGCTGATCCTGCCTGATGGTGGCCAACTCCACCTAGAGAGCACCGATCACGA
GACCGTGCCCGGTTTTCCACACCCCCTGCGCGTGCGTGCCCGCCGTGGAGGAGAGCGCTTGATCTTGCCTGGGCGTACCC
ATTCCCACCCCCTCAAACACCTCTTGCAAGACGTCGGCATCCCCCCCTGGCGGCGTGCATCAATGCCCTTATTGTGCGAC
GGTGAACAGATCCTCGCCGTTGGCGATGCCCTACTGGCCGCACCACTGGTCACCTGGTTGCAGGCTCATCGCCTCAAACT
ACGCTGGCAATACCACAACAACACATGCATTTGA

Upstream 100 bases:

>100_bases
GTGCCTGTGTATTTGAATACACCAGGAGACACACTCCCTACCGCCGCTGGCGGCTCGCTGAGTCAGCAGAGATACTGCAT
CCCCATTTCACCCCCCTGCA

Downstream 100 bases:

>100_bases
CCATCCCGATGACACCCCGCACACTTGCCCGATGCCAAAACGCTCCCCGCCAGACACCTCCCCCGTTGCCCGCTTTGAAC
AGTCTCTTCAAGAATTGGAG

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTVSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWTAHCAAFCTQHDIRLHIAR
VHVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHS
RAQLLDYAQQQHLDWIEDSSNADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDG
ALDINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIRRWRLRLYLHRPQPPWPPD
WQPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCD
GEQILAVGDALLAAPLVTWLQAHRLKLRWQYHNNTCI

Sequences:

>Translated_437_residues
MTVSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWTAHCAAFCTQHDIRLHIAR
VHVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHS
RAQLLDYAQQQHLDWIEDSSNADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDG
ALDINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIRRWRLRLYLHRPQPPWPPD
WQPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCD
GEQILAVGDALLAAPLVTWLQAHRLKLRWQYHNNTCI
>Mature_436_residues
TVSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADAWTAHCAAFCTQHDIRLHIARV
HVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCDDQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSR
AQLLDYAQQQHLDWIEDSSNADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDGA
LDINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTEIRRWRLRLYLHRPQPPWPPDW
QPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRARRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDG
EQILAVGDALLAAPLVTWLQAHRLKLRWQYHNNTCI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=400, Percent_Identity=41.75, Blast_Score=253, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 48764; Mature: 48633

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADA
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCHH
WTAHCAAFCTQHDIRLHIARVHVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCD
HHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRAQLLDYAQQQHLDWIEDSS
CHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
NADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDG
CCCCHHCCCEEEEEEEHHHHHCCCHHHHHHHHCHHHEECCCCCCCCCCCEECCCCCCCCC
ALDINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTE
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHCCCCCCCCEEEEECHHH
IRRWRLRLYLHRPQPPWPPDWQPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRA
HHEEEEEEEEECCCCCCCCCCCHHHCCCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHC
RRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGEQILAVGDALLAAPLVTWL
CCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
QAHRLKLRWQYHNNTCI
HHHCEEEEEEECCCCCC
>Mature Secondary Structure 
TVSSPFPRIPDPGVPVLVAFSGGLDSTVLLHCLASQPTQRIHGLEAIHIHHGLNENADA
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHCCCEEEEEECCCCCCCHH
WTAHCAAFCTQHDIRLHIARVHVSHNSGQGLEAAARTARRAAFAHTLQHGHYLALAHHCD
HHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
DQAETWLLRALRGSCDGLAAMRPLTPFAAGHLWRPLLTHSRAQLLDYAQQQHLDWIEDSS
CHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
NADLRHDRNFLRIHVLPLLHQRWPQATAVLARNAALAAANADLLNAEDAVLLPDLLDPDG
CCCCHHCCCEEEEEEEHHHHHCCCHHHHHHHHCHHHEECCCCCCCCCCCEECCCCCCCCC
ALDINALTAHPPARRARLLRAWCARAGAPPLPERGVNIIERELLPARHDSAACFTWSHTE
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHCCCCCCCCEEEEECHHH
IRRWRLRLYLHRPQPPWPPDWQPLWSGTAPLILPDGGQLHLESTDHETVPGFPHPLRVRA
HHEEEEEEEEECCCCCCCCCCCHHHCCCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHC
RRGGERLILPGRTHSHPLKHLLQDVGIPPWRRASMPLLCDGEQILAVGDALLAAPLVTWL
CCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHH
QAHRLKLRWQYHNNTCI
HHHCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]