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The map label for this gene is rumA [H]

Identifier: 28199269

GI number: 28199269

Start: 1607330

End: 1608661

Strand: Reverse

Name: rumA [H]

Synonym: PD1385

Alternate gene names: 28199269

Gene position: 1608661-1607330 (Counterclockwise)

Preceding gene: 28199271

Following gene: 28199268

Centisome position: 63.84

GC content: 52.55

Gene sequence:

>1332_bases
GTGGCTCGCCAAAATCGTTTCGATCGTACGTCATTTCAAACGCAGATTATTGATCTCAGTCATGATGGGCGTGGGGTTGC
ACGACCTCATGGCGAGGGAGGTAAGGTGACTTTCGTCACTGGCGCGCTTCCAGGTGAGGTGGTGATTGTTGAGCCAGTTG
CGCGTAATCGTCATTTTGATGAGGCACGTGTCGTTGAGGTGTTGCAGGCATCGCCGCAACGGGTGATACCGCGTTGCTCG
CATTTTGGTGTCTGTTCTGGTTGTGTATTGCAGCATTTGGCTGAAGATGCGCAGGTTGTGTCGAAGCAACGGGTGTTGTT
GGAAAGTTTGGAACGTATTGGTTGTGTCAGTCCGGAGCGTGTGTTGCCGGCGTTGGCCGCGGAGAGCTGGGGGTATCGGC
GTAAGGGGCGTTTTTCTGTACGTTGGGTTGAGAAAAAGGGGAGGACGCTGGTTGGTTTCCGTGAGCATGATCCGCGTTTT
GTTGCCGATGTGTCGGTCTGTCATACAGTGGTTCCGCAGGTCGGTGAAAAAATTGCGCTTTTGGCAACGTTGCTTGACAG
TTTGGACGGTAGGTGTGACGTACCGCAGATTGAGTTTATTGCCGGTGATGCTGTTGTGGCATTAACGGTGCGTCATTTGC
AGCCGTTGAGTGAGGCGGATCGTTTGGCGCTGATTGAGTTTGGCAAGGAGCATGGTATTGCAATTTTTTTGCAGTCGGGT
GGGGTGGAGAGTGTGCGTTTGTTGTGGCCTGATGAGGTGTTGTTGGCATTTCGCTTGAAGCCTTGGGATGTGGAGTTTGT
GTTCCGTCCGCTGGATTTCATTCAGATCAATGGTGGGCTGAATGAGAAGATGATTGCGCATGCTTTGGATCTGCTCGGTG
CTGGATTTGGGGAGCGTGTGCTCGATCTGTTTTGTGGTTTGGGTAACTTCACGTTGCCGTTGGCGCGTACTGTGGGTGAG
GTGGTTGGAGTGGAGGGTGATATTGGGCTGGTGGAGCGGGCCAGGGAGAATGCACGGCGTAATGGTTTGGGGAATGCTGA
ATTTTTTGTTGCTGATTTGACCCGTGATCAACGCGATGCGCCGTGGATGCGTCAGGGCTTTGATAAGTTGCTGTTGGATC
CGCCGCGTTCCGGAGCGATTGAGGTACTCAAGCAGTTGCCGTTAAAGATGTTCGAACGCATCGTTTATGTCAGTTGTCAT
CCTGGATCTCTGGCGCGTGATGCTGATTTCCTTGTCAATGAACAGGGCTTTGTGTTGCGTGCGGTTGGGGCGATGGACAT
GTTTCCGCATACTGCGCATGTGGAGAGTATTGCTGTATTTGATCGTTGCTGA

Upstream 100 bases:

>100_bases
CTTTATTTTTTGTTTGGATGGATATGTGGATGGTGGGCTGGGTTCAGTATGGAACGCAATCTGCGACAATGTTTCGAATC
TTATTTGCAGTGTTTCTTTT

Downstream 100 bases:

>100_bases
GTGATCCGAAGAGTGTTGCACGGTCTGATTCGCTCTGACTTTTTCTGAATTTTATAAAATTATGGCTATCGAAATAGAAC
GTAAATTCCTGGTTGTTGGG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVARNRHFDEARVVEVLQASPQRVIPRCS
HFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFREHDPRF
VADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQPLSEADRLALIEFGKEHGIAIFLQSG
GVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAGFGERVLDLFCGLGNFTLPLARTVGE
VVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIVYVSCH
PGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC

Sequences:

>Translated_443_residues
MARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVARNRHFDEARVVEVLQASPQRVIPRCS
HFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFREHDPRF
VADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQPLSEADRLALIEFGKEHGIAIFLQSG
GVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAGFGERVLDLFCGLGNFTLPLARTVGE
VVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIVYVSCH
PGSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC
>Mature_442_residues
ARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVARNRHFDEARVVEVLQASPQRVIPRCSH
FGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPERVLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFREHDPRFV
ADVSVCHTVVPQVGEKIALLATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQPLSEADRLALIEFGKEHGIAIFLQSGG
VESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAGFGERVLDLFCGLGNFTLPLARTVGEV
VGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDAPWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIVYVSCHP
GSLARDADFLVNEQGFVLRAVGAMDMFPHTAHVESIAVFDRC

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=194, Percent_Identity=34.5360824742268, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI51173878, Length=194, Percent_Identity=34.5360824742268, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789148, Length=445, Percent_Identity=38.4269662921348, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=29.1891891891892, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787083, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=32.3232323232323, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 49101; Mature: 48970

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVARNRHFD
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHCCCCCC
EARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPER
HHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFREHDPRFVADVSVCHTVVPQVGEKIAL
HHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHH
LATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQPLSEADRLALIEFGKEHGIAIFLQSG
HHHHHHHCCCCCCCCCEEEEECCEEEEHHHHHHCCCCCCCCEEEEHCCCCCCEEEEEECC
GVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAGFGERV
CCCEEEEECCCCEEEEEECCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHHH
LDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDA
HHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
PWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIVYVSCHPGSLARDADFLVNEQGFVLR
CHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHEECCCCCEEE
AVGAMDMFPHTAHVESIAVFDRC
EECHHHCCCCHHHHHHEECCCCC
>Mature Secondary Structure 
ARQNRFDRTSFQTQIIDLSHDGRGVARPHGEGGKVTFVTGALPGEVVIVEPVARNRHFD
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHCCCCCC
EARVVEVLQASPQRVIPRCSHFGVCSGCVLQHLAEDAQVVSKQRVLLESLERIGCVSPER
HHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VLPALAAESWGYRRKGRFSVRWVEKKGRTLVGFREHDPRFVADVSVCHTVVPQVGEKIAL
HHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHH
LATLLDSLDGRCDVPQIEFIAGDAVVALTVRHLQPLSEADRLALIEFGKEHGIAIFLQSG
HHHHHHHCCCCCCCCCEEEEECCEEEEHHHHHHCCCCCCCCEEEEHCCCCCCEEEEEECC
GVESVRLLWPDEVLLAFRLKPWDVEFVFRPLDFIQINGGLNEKMIAHALDLLGAGFGERV
CCCEEEEECCCCEEEEEECCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHHH
LDLFCGLGNFTLPLARTVGEVVGVEGDIGLVERARENARRNGLGNAEFFVADLTRDQRDA
HHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
PWMRQGFDKLLLDPPRSGAIEVLKQLPLKMFERIVYVSCHPGSLARDADFLVNEQGFVLR
CHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHEECCCCCEEE
AVGAMDMFPHTAHVESIAVFDRC
EECHHHCCCCHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]